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Article ; Online: Spiders in space-orb-web-related behaviour in zero gravity.

Zschokke, Samuel / Countryman, Stefanie / Cushing, Paula E

2020 Volume 108, Issue 1, Page(s) 1

Abstract: Gravity is very important for many organisms, including web-building spiders. Probably the best approach to study the relevance of gravity on organisms is to bring them to the International Space Station. Here, we describe the results of such an ... ...

Abstract	Gravity is very important for many organisms, including web-building spiders. Probably the best approach to study the relevance of gravity on organisms is to bring them to the International Space Station. Here, we describe the results of such an experiment where two juvenile Trichonephila clavipes (L.) (Araneae, Nephilidae) spiders were observed over a 2-month period in zero gravity and two control spiders under otherwise identical conditions on Earth. During that time, the spiders and their webs were photographed every 5 min. Under natural conditions, Trichonephila spiders build asymmetric webs with the hub near the upper edge of the web, and they always orient themselves downwards when sitting on the hub whilst waiting for prey. As these asymmetries are considered to be linked to gravity, we expected the spiders experiencing no gravity to build symmetric webs and to show a random orientation when sitting on the hub. We found that most, but not all, webs built in zero gravity were indeed quite symmetric. Closer analysis revealed that webs built when the lights were on were more asymmetric (with the hub near the lights) than webs built when the lights were off. In addition, spiders showed a random orientation when the lights were off but faced away from the lights when they were on. We conclude that in the absence of gravity, the direction of light can serve as an orientation guide for spiders during web building and when waiting for prey on the hub.
MeSH term(s)	Animals ; Darkness ; Light ; Nesting Behavior/physiology ; Nesting Behavior/radiation effects ; Spiders/physiology ; Spiders/radiation effects ; Weightlessness
Language	English
Publishing date	2020-12-03
Publishing country	Germany
Document type	Journal Article
ZDB-ID	123257-5
ISSN	1432-1904 ; 0028-1042
ISSN (online)	1432-1904
ISSN	0028-1042
DOI	10.1007/s00114-020-01708-8
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Zs.A 664: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Ui VI 2855: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)

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Article ; Online: Providing Palliative Care to Patients Throughout the State of Indiana from a Centralized Virtual Palliative Care Hub.

Shepard, Eric N / Patel, Ami V / Countryman, Shannon / Kara, Areeba / Sinha, Shilpee

Joint Commission journal on quality and patient safety

2022 Volume 48, Issue 12, Page(s) 635–641

Abstract: Background: Access to palliative care clinicians is a limited resource. Expanding the reach of existing palliative care expertise by utilizing virtual care is one strategy to reach areas that lack access. We delivered virtual services through a ... ...

Abstract	Background: Access to palliative care clinicians is a limited resource. Expanding the reach of existing palliative care expertise by utilizing virtual care is one strategy to reach areas that lack access. We delivered virtual services through a centralized hub across multiple health settings and tracked outcomes. Methods: Through a centralized virtual palliative care hub based in an urban academic health center, access to specialty palliative care was offered across homes, critical access hospitals (CAHs), and extended care facilities (ECFs) in the state of Indiana. Webpage-based platforms were used, and hardware included a cart on wheels for rural hospital sites. Data specific to palliative care were monitored for each encounter. Results: Over one year, 372 patients were seen for virtual palliative care consultations, of whom 275 (73.9%) were seen in the outpatient setting (where the patient was at home or in an ECF) and 97 (26.1%) were inpatient visits performed in CAHs. Visits occurred with patients from almost all counties in Indiana. Advance directives were established for 286 (76.9%) patients seen, and 107 (28.8%) patients were referred to hospice. Conclusion: Specialty palliative care is a limited resource that has been further constrained by the COVID-19 pandemic. Our experience demonstrates that centralized virtual hub-based palliative care can be leveraged to provide effective, patient-centered, and compassionate care in regions without a specialist and has the potential to improve access to specialty palliative care.
MeSH term(s)	Humans ; Palliative Care ; Indiana ; Pandemics ; COVID-19/therapy ; Advance Directives
Language	English
Publishing date	2022-08-23
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1189890-2
ISSN	1938-131X ; 1549-425X ; 1553-7250 ; 1070-3241 ; 1549-3741
ISSN (online)	1938-131X ; 1549-425X
ISSN	1553-7250 ; 1070-3241 ; 1549-3741
DOI	10.1016/j.jcjq.2022.08.008
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Zs.B 2055: Show issues

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Article: Impact of microgravity on a three-dimensional microphysiologic culture of the human kidney proximal tubule epithelium: cell response to serum and vitamin D.

Kelly, Edward / Lindberg, Kevin / Jones-Isaac, Kendan / Yang, Jade / Bain, Jacelyn / Wang, Lu / MacDonald, James / Bammler, Theo / Calamia, Justina / Thummel, Kenneth / Yeung, Catherine / Countryman, Stefanie / Koenig, Paul / Himmelfarb, Jonathan

Research square

2023

Abstract: The microgravity environment aboard the International Space Station (ISS) provides a unique stressor that can help understand underlying cellular and molecular drivers of pathological changes observed in astronauts with the ultimate goals of developing ... ...

Abstract	The microgravity environment aboard the International Space Station (ISS) provides a unique stressor that can help understand underlying cellular and molecular drivers of pathological changes observed in astronauts with the ultimate goals of developing strategies to enable long-term spaceflight and better treatment of diseases on Earth. We used this unique environment to evaluate the effects of microgravity on kidney proximal tubule epithelial cell (PTEC) response to serum exposure and vitamin D biotransformation capacity. To test if microgravity alters the pathologic response of the proximal tubule to serum exposure, we treated PTECs cultured in a microphysiological system (PT-MPS) with human serum and measured biomarkers of toxicity and inflammation (KIM-1 and IL-6) and conducted global transcriptomics via RNAseq on cells undergoing flight (microgravity) and respective controls (ground). We also treated 3D cultured PTECs with 25(OH)D
Language	English
Publishing date	2023-12-21
Publishing country	United States
Document type	Preprint
DOI	10.21203/rs.3.rs-3778779/v1
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Article: Development of a Kidney Microphysiological System Hardware Platform for Microgravity Studies.

Yeung, Catherine / Jones-Isaac, Kendan / Lindberg, Kevin / Yang, Jade / Bain, Jacelyn / Ruiz, Micaela / Koenig, Greta / Koenig, Paul / Countryman, Stefanie / Himmelfarb, Jonathan / Kelly, Edward

Research square

2023

Abstract: Study of the physiological effects of microgravity on humans is limited to non-invasive testing of astronauts. Microphysiological models of human organs recapitulate many functions and disease states. Here we describe the development of an advanced, semi- ...

Abstract	Study of the physiological effects of microgravity on humans is limited to non-invasive testing of astronauts. Microphysiological models of human organs recapitulate many functions and disease states. Here we describe the development of an advanced, semi-autonomous hardware platform to support kidney microphysiological model experiments in microgravity.
Language	English
Publishing date	2023-12-22
Publishing country	United States
Document type	Preprint
DOI	10.21203/rs.3.rs-3750478/v1
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Article ; Online: CRISPR-based genetic diagnostics in microgravity.

Alon, Dan Mark / Mittelman, Karin / Stibbe, Eytan / Countryman, Stefanie / Stodieck, Louis / Doraisingam, Shankini / Leal Martin, Dylan Mikeala / Hamo, Eliran Raphael / Pines, Gur / Burstein, David

Biosensors & bioelectronics

2023 Volume 237, Page(s) 115479

Abstract: Monitoring astronauts' health during space missions poses many challenges, including rapid assessment of crew health conditions. Sensitive genetic diagnostics are crucial for examining crew members and the spacecraft environment. CRISPR-Cas12a, coupled ... ...

Abstract	Monitoring astronauts' health during space missions poses many challenges, including rapid assessment of crew health conditions. Sensitive genetic diagnostics are crucial for examining crew members and the spacecraft environment. CRISPR-Cas12a, coupled with isothermal amplification, has proven to be a promising biosensing system for rapid, on-site detection of genomic targets. However, the efficiency and sensitivity of CRISPR-based diagnostics have never been tested in microgravity. We tested the use of recombinase polymerase amplification (RPA) coupled with the collateral cleavage activity of Cas12a for genetic diagnostics onboard the International Space Station. We explored the detection sensitivity of amplified and unamplified target DNA. By coupling RPA with Cas12a, we identified targets in attomolar concentrations. We further assessed the reactions' stability following long-term storage. Our results demonstrate that CRISPR-based detection is a powerful tool for on-site genetic diagnostics in microgravity, and can be further utilized for long-term space endeavors to improve astronauts' health and well-being.
MeSH term(s)	Humans ; Weightlessness ; Biosensing Techniques ; Astronauts ; Genomics ; Recombinases ; CRISPR-Cas Systems/genetics ; Nucleic Acid Amplification Techniques
Chemical Substances	Recombinases
Language	English
Publishing date	2023-07-05
Publishing country	England
Document type	Journal Article
ZDB-ID	1011023-9
ISSN	1873-4235 ; 0956-5663
ISSN (online)	1873-4235
ISSN	0956-5663
DOI	10.1016/j.bios.2023.115479
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Zs.A 2275: Show issues

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Article ; Online: Tissue Chips in Space-Challenges and Opportunities.

Yeung, Catherine K / Koenig, Paul / Countryman, Stefanie / Thummel, Kenneth E / Himmelfarb, Jonathan / Kelly, Edward J

Clinical and translational science

2019 Volume 13, Issue 1, Page(s) 8–10

MeSH term(s)	Aging/physiology ; Ecological Systems, Closed ; Humans ; Kidney Tubules, Proximal/cytology ; Microfluidic Analytical Techniques/instrumentation ; Primary Cell Culture/instrumentation ; Primary Cell Culture/methods ; Spacecraft ; Translational Medical Research/instrumentation ; Translational Medical Research/methods ; Weightlessness/adverse effects
Language	English
Publishing date	2019-09-16
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2433157-0
ISSN	1752-8062 ; 1752-8054
ISSN (online)	1752-8062
ISSN	1752-8054
DOI	10.1111/cts.12689
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Article: Osteoprotegerin and bone loss associated with spaceflight.

Bateman, Ted A / Countryman, S

Drug discovery today

2002 Volume 7, Issue 8, Page(s) 456–457

MeSH term(s)	Apoptosis/physiology ; Bone Diseases/chemically induced ; Bone Diseases/etiology ; Bone and Bones/cytology ; Bone and Bones/physiology ; Cytokines/physiology ; Glycoproteins/physiology ; Glycoproteins/therapeutic use ; Humans ; Osteoprotegerin ; Receptors, Cytoplasmic and Nuclear/physiology ; Receptors, Cytoplasmic and Nuclear/therapeutic use ; Receptors, Tumor Necrosis Factor ; Space Flight ; Weightlessness/adverse effects
Chemical Substances	Cytokines ; Glycoproteins ; Osteoprotegerin ; Receptors, Cytoplasmic and Nuclear ; Receptors, Tumor Necrosis Factor ; TNFRSF11B protein, human
Language	English
Publishing date	2002-04-15
Publishing country	England
Document type	Letter
ZDB-ID	1324988-5
ISSN	1878-5832 ; 1359-6446
ISSN (online)	1878-5832
ISSN	1359-6446
DOI	10.1016/s1359-6446(02)02260-2
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Zs.A 4725: Show issues

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Article ; Online: Movantik™ and the Frequency of Positive Naloxone in Urine.

Wotring, Justin / Countryman, Sky / Wallace, Frank N / Strickland, Erin C / Cummings, Oneka T / McIntire, Gregory L

Journal of analytical toxicology

2018 Volume 42, Issue 3, Page(s) e38–e40

MeSH term(s)	Humans ; Morphinans/urine ; Naloxone/urine ; Narcotic Antagonists/urine ; Polyethylene Glycols ; Substance Abuse Detection/methods
Chemical Substances	Morphinans ; Narcotic Antagonists ; Naloxone (36B82AMQ7N) ; Polyethylene Glycols (3WJQ0SDW1A) ; naloxegol (44T7335BKE)
Language	English
Publishing date	2018-01-05
Publishing country	England
Document type	Journal Article
ZDB-ID	752391-9
ISSN	1945-2403 ; 0146-4760
ISSN (online)	1945-2403
ISSN	0146-4760
DOI	10.1093/jat/bkx104
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Zs.A 1333: Show issues

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Article: Feasibility, potency, and safety of growing human mesenchymal stem cells in space for clinical application.

Huang, Peng / Russell, Athena L / Lefavor, Rebecca / Durand, Nisha C / James, Elle / Harvey, Larry / Zhang, Cuiping / Countryman, Stefanie / Stodieck, Louis / Zubair, Abba C

NPJ microgravity

2020 Volume 6, Page(s) 16

Abstract: Growing stem cells on Earth is very challenging and limited to a few population doublings. The standard two-dimensional (2D) culture environment is an unnatural condition for cell growth. Therefore, culturing stem cells aboard the International Space ... ...

Abstract	Growing stem cells on Earth is very challenging and limited to a few population doublings. The standard two-dimensional (2D) culture environment is an unnatural condition for cell growth. Therefore, culturing stem cells aboard the International Space Station (ISS) under a microgravity environment may provide a more natural three-dimensional environment for stem cell expansion and organ development. In this study, human-derived mesenchymal stem cells (MSCs) grown in space were evaluated to determine their potential use for future clinical applications on Earth and during long-term spaceflight. MSCs were flown in Plate Habitats for transportation to the ISS. The MSCs were imaged every 24-48 h and harvested at 7 and 14 days. Conditioned media samples were frozen at -80 °C and cells were either cryopreserved in 5% dimethyl sulfoxide, RNAprotect, or paraformaldehyde. After return to Earth, MSCs were characterized to establish their identity and cell cycle status. In addition, cell proliferation, differentiation, cytokines, and growth factors' secretion were assessed. To evaluate the risk of malignant transformation, the space-grown MSCs were subjected to chromosomal, DNA damage, and tumorigenicity assays. We found that microgravity had significant impact on the MSC capacity to secrete cytokines and growth factors. They appeared to be more potent in terms of immunosuppressive capacity compared to their identical ground control. Chromosomal, DNA damage, and tumorigenicity assays showed no evidence of malignant transformation. Therefore, it is feasible and potentially safe to grow MSCs aboard the ISS for potential future clinical applications.
Language	English
Publishing date	2020-06-01
Publishing country	United States
Document type	Journal Article
ZDB-ID	2823626-9
ISSN	2373-8065
ISSN	2373-8065
DOI	10.1038/s41526-020-0106-z
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Article ; Online: Effects of Spaceflight on Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Structure and Function.

Wnorowski, Alexa / Sharma, Arun / Chen, Haodong / Wu, Haodi / Shao, Ning-Yi / Sayed, Nazish / Liu, Chun / Countryman, Stefanie / Stodieck, Louis S / Rubins, Kathleen H / Wu, Sean M / Lee, Peter H U / Wu, Joseph C

Stem cell reports

2019 Volume 13, Issue 6, Page(s) 960–969

Abstract: With extended stays aboard the International Space Station (ISS) becoming commonplace, there is a need to better understand the effects of microgravity on cardiac function. We utilized human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) ...

Abstract	With extended stays aboard the International Space Station (ISS) becoming commonplace, there is a need to better understand the effects of microgravity on cardiac function. We utilized human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) to study the effects of microgravity on cell-level cardiac function and gene expression. The hiPSC-CMs were cultured aboard the ISS for 5.5 weeks and their gene expression, structure, and functions were compared with ground control hiPSC-CMs. Exposure to microgravity on the ISS caused alterations in hiPSC-CM calcium handling. RNA-sequencing analysis demonstrated that 2,635 genes were differentially expressed among flight, post-flight, and ground control samples, including genes involved in mitochondrial metabolism. This study represents the first use of hiPSC technology to model the effects of spaceflight on human cardiomyocyte structure and function.
MeSH term(s)	Biomarkers ; Calcium/metabolism ; Cell Culture Techniques ; Cell Differentiation ; Cells, Cultured ; Computational Biology/methods ; Energy Metabolism ; Fluorescent Antibody Technique ; Gene Expression Profiling ; Humans ; Induced Pluripotent Stem Cells/cytology ; Induced Pluripotent Stem Cells/metabolism ; Molecular Sequence Annotation ; Myocytes, Cardiac/cytology ; Myocytes, Cardiac/metabolism ; Space Flight ; Weightlessness
Chemical Substances	Biomarkers ; Calcium (SY7Q814VUP)
Language	English
Publishing date	2019-11-07
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2720528-9
ISSN	2213-6711 ; 2213-6711
ISSN (online)	2213-6711
ISSN	2213-6711
DOI	10.1016/j.stemcr.2019.10.006
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