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  1. Article: Heterologous production of chondroitin.

    Couto, Márcia R / Rodrigues, Joana L / Rodrigues, Lígia R

    Biotechnology reports (Amsterdam, Netherlands)

    2022  Volume 33, Page(s) e00710

    Abstract: Chondroitin sulfate (CS) is a glycosaminoglycan with a broad range of applications being a popular dietary supplement for osteoarthritis. Usually, CS is extracted from animal sources. However, the known risks of animal products use have been driving the ... ...

    Abstract Chondroitin sulfate (CS) is a glycosaminoglycan with a broad range of applications being a popular dietary supplement for osteoarthritis. Usually, CS is extracted from animal sources. However, the known risks of animal products use have been driving the search for alternative methods and sources to obtain this compound. Several pathogenic bacteria naturally produce chondroitin-like polysaccharides through well-known pathways and, therefore, have been the basis for numerous studies that aim to produce chondroitin using non-pathogenic hosts. However, the yields obtained are not enough to meet the high demand for this glycosaminoglycan. Metabolic engineering strategies have been used to construct improved heterologous hosts. The identification of metabolic bottlenecks and regulation points, and the screening for efficient enzymes are key points for constructing microbial cell factories with improved chondroitin yields to achieve industrial CS production. The recent advances on enzymatic and microbial strategies to produce non-animal chondroitin are herein reviewed. Challenges and prospects for future research are also discussed.
    Language English
    Publishing date 2022-02-10
    Publishing country Netherlands
    Document type Journal Article ; Review
    ZDB-ID 2801018-8
    ISSN 2215-017X
    ISSN 2215-017X
    DOI 10.1016/j.btre.2022.e00710
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Cloning, Expression and Characterization of UDP-Glucose Dehydrogenases.

    Couto, Márcia R / Rodrigues, Joana L / Rodrigues, Lígia R

    Life (Basel, Switzerland)

    2021  Volume 11, Issue 11

    Abstract: Uridine diphosphate-glucose dehydrogenase (UGD) is an enzyme that produces uridine diphosphate-glucuronic acid (UDP-GlcA), which is an intermediate in glycosaminoglycans (GAGs) production pathways. GAGs are generally extracted from animal tissues. ... ...

    Abstract Uridine diphosphate-glucose dehydrogenase (UGD) is an enzyme that produces uridine diphosphate-glucuronic acid (UDP-GlcA), which is an intermediate in glycosaminoglycans (GAGs) production pathways. GAGs are generally extracted from animal tissues. Efforts to produce GAGs in a safer way have been conducted by constructing artificial biosynthetic pathways in heterologous microbial hosts. This work characterizes novel enzymes with potential for UDP-GlcA biotechnological production. The UGD enzymes from
    Language English
    Publishing date 2021-11-07
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662250-6
    ISSN 2075-1729
    ISSN 2075-1729
    DOI 10.3390/life11111201
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Heterologous production of chondroitin

    Couto, Márcia R. / Rodrigues, Joana L. / Rodrigues, Lígia R.

    Biotechnology reports. 2022 Mar., v. 33

    2022  

    Abstract: Chondroitin sulfate (CS) is a glycosaminoglycan with a broad range of applications being a popular dietary supplement for osteoarthritis. Usually, CS is extracted from animal sources. However, the known risks of animal products use have been driving the ... ...

    Abstract Chondroitin sulfate (CS) is a glycosaminoglycan with a broad range of applications being a popular dietary supplement for osteoarthritis. Usually, CS is extracted from animal sources. However, the known risks of animal products use have been driving the search for alternative methods and sources to obtain this compound. Several pathogenic bacteria naturally produce chondroitin-like polysaccharides through well-known pathways and, therefore, have been the basis for numerous studies that aim to produce chondroitin using non-pathogenic hosts. However, the yields obtained are not enough to meet the high demand for this glycosaminoglycan. Metabolic engineering strategies have been used to construct improved heterologous hosts. The identification of metabolic bottlenecks and regulation points, and the screening for efficient enzymes are key points for constructing microbial cell factories with improved chondroitin yields to achieve industrial CS production. The recent advances on enzymatic and microbial strategies to produce non-animal chondroitin are herein reviewed. Challenges and prospects for future research are also discussed.
    Keywords animals ; biotechnology ; chondroitin sulfate ; dietary supplements ; osteoarthritis
    Language English
    Dates of publication 2022-03
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 2801018-8
    ISSN 2215-017X
    ISSN 2215-017X
    DOI 10.1016/j.btre.2022.e00710
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: Optimization of fermentation conditions for the production of curcumin by engineered

    Couto, Márcia R / Rodrigues, Joana L / Rodrigues, Lígia R

    Journal of the Royal Society, Interface

    2017  Volume 14, Issue 133

    Abstract: Curcumin is a plant secondary metabolite with outstanding therapeutic effects. Therefore, there is a great interest in developing new strategies to produce this high-value compound in a cheaper and environmentally friendly way. Curcumin heterologous ... ...

    Abstract Curcumin is a plant secondary metabolite with outstanding therapeutic effects. Therefore, there is a great interest in developing new strategies to produce this high-value compound in a cheaper and environmentally friendly way. Curcumin heterologous production in
    MeSH term(s) Curcumin/metabolism ; Escherichia coli K12/genetics ; Escherichia coli K12/growth & development ; Metabolic Engineering
    Chemical Substances Curcumin (IT942ZTH98)
    Language English
    Publishing date 2017-08-20
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2156283-0
    ISSN 1742-5662 ; 1742-5689
    ISSN (online) 1742-5662
    ISSN 1742-5689
    DOI 10.1098/rsif.2017.0470
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: The biopolymer produced by Rhizobium viscosum CECT 908 is a promising agent for application in microbial enhanced oil recovery

    Couto, Márcia R / Débora Ferreira / Eduardo J. Gudiña / José A. Teixeira / Lígia R. Rodrigues

    New biotechnology. 2019 Mar. 25, v. 49

    2019  

    Abstract: Polymer flooding is one of the most promising techniques used to increase the productivity of mature oil reservoirs. Polymers reduce the mobility ratio of the injected water relative to the crude oil, improving the displacement of the entrapped oil and ... ...

    Abstract Polymer flooding is one of the most promising techniques used to increase the productivity of mature oil reservoirs. Polymers reduce the mobility ratio of the injected water relative to the crude oil, improving the displacement of the entrapped oil and consequently, increasing oil recovery. Biopolymers such as xanthan gum have emerged as environmentally friendly alternatives to the chemical polymers commonly employed by the oil industry. However, in order to seek more efficient biomolecules, alternative biopolymers must be studied. Here, the applicability of a biopolymer produced by Rhizobium viscosum CECT 908 in Microbial Enhanced Oil Recovery (MEOR) was evaluated. This biopolymer exhibited better rheological properties (including higher viscosity) when compared with xanthan gum. Its stability at high shear rates (up to 300 s−1), temperatures (up to 80 °C) and salinities (up to 200 g/L of NaCl) was also demonstrated. The biopolymer exhibited better performance than xanthan gum in oil recovery assays performed with a heavy crude oil, achieving 25.7 ± 0.5% of additional recovery. Thus the R. viscosum CECT 908 biopolymer is a promising candidate for application in MEOR.
    Keywords biopolymers ; industry ; oil fields ; oils ; petroleum ; Rhizobium ; salinity ; sodium chloride ; temperature ; viscosity ; xanthan gum
    Language English
    Dates of publication 2019-0325
    Size p. 144-150.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 2400836-9
    ISSN 1876-4347 ; 1871-6784
    ISSN (online) 1876-4347
    ISSN 1871-6784
    DOI 10.1016/j.nbt.2018.11.002
    Database NAL-Catalogue (AGRICOLA)

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  6. Article ; Online: The biopolymer produced by Rhizobium viscosum CECT 908 is a promising agent for application in microbial enhanced oil recovery.

    Couto, Márcia R / Gudiña, Eduardo J / Ferreira, Débora / Teixeira, José A / Rodrigues, Lígia R

    New biotechnology

    2018  Volume 49, Page(s) 144–150

    Abstract: Polymer flooding is one of the most promising techniques used to increase the productivity of mature oil reservoirs. Polymers reduce the mobility ratio of the injected water relative to the crude oil, improving the displacement of the entrapped oil and ... ...

    Abstract Polymer flooding is one of the most promising techniques used to increase the productivity of mature oil reservoirs. Polymers reduce the mobility ratio of the injected water relative to the crude oil, improving the displacement of the entrapped oil and consequently, increasing oil recovery. Biopolymers such as xanthan gum have emerged as environmentally friendly alternatives to the chemical polymers commonly employed by the oil industry. However, in order to seek more efficient biomolecules, alternative biopolymers must be studied. Here, the applicability of a biopolymer produced by Rhizobium viscosum CECT 908 in Microbial Enhanced Oil Recovery (MEOR) was evaluated. This biopolymer exhibited better rheological properties (including higher viscosity) when compared with xanthan gum. Its stability at high shear rates (up to 300 s
    MeSH term(s) Biopolymers/biosynthesis ; Oils/isolation & purification ; Rheology ; Rhizobium/metabolism ; Shear Strength ; Temperature ; Time Factors ; Viscosity
    Chemical Substances Biopolymers ; Oils
    Language English
    Publishing date 2018-11-13
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2400836-9
    ISSN 1876-4347 ; 1871-6784
    ISSN (online) 1876-4347
    ISSN 1871-6784
    DOI 10.1016/j.nbt.2018.11.002
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Kategorien

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