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  1. Article ; Online: Genetic landscape clustering of a large DNA barcoding data set reveals shared patterns of genetic divergence among freshwater fishes of the Maroni Basin.

    Papa, Yvan / Le Bail, Pierre-Yves / Covain, Raphaël

    Molecular ecology resources

    2021  Volume 21, Issue 6, Page(s) 2109–2124

    Abstract: The Maroni is one of the most speciose basins of the Guianas and hosts a megadiverse freshwater fish community. Although taxonomic references based on morphological identification exist for both the Surinamese and Guianese parts of the basin, there are ... ...

    Abstract The Maroni is one of the most speciose basins of the Guianas and hosts a megadiverse freshwater fish community. Although taxonomic references based on morphological identification exist for both the Surinamese and Guianese parts of the basin, there are still taxonomic uncertainties concerning the status of several species. We used COI sequences of 1284 fish in conjunction with morphological and biogeographical evidence to assist with species delineation and discovery in order to validate and standardize the current taxonomy. This resulted in a final DNA barcode data set of 199 fish species (125 genera, 36 families and eight orders; 68.86% of strictly freshwater fishes from the basin), among which 25 are new putative candidate species flagged as requiring taxonomic update. DNA barcoding delineation through Barcode Index Numbers (BINs) revealed further cryptic diversity (230 BINs in total). To explore global genetic patterns across the basin, genetic divergence landscapes were computed for 128 species, showing a global trend of high genetic divergence between the Surinamese southwest (Tapanahony and Paloemeu), the Guianese southeast (Marouini, Litany, Tampok, etc.), and the river outlet in the north. This could be explained by lower levels of connectivity between these three main areas and/or the exchange of individuals between these areas and the neighbouring basins. A new method of ordination of genetic landscapes successfully assigned species into cluster groups based on their respective pattern of genetic divergence across the Maroni Basin: genetically homogeneous species were effectively discriminated from species showing high spatial genetic fragmentation and possible lower capacity for dispersal.
    MeSH term(s) Animals ; Biodiversity ; Cluster Analysis ; DNA Barcoding, Taxonomic ; Electron Transport Complex IV/genetics ; Fishes/classification ; Fishes/genetics ; French Guiana ; Fresh Water ; Genetic Variation ; Phylogeny ; Suriname
    Chemical Substances Electron Transport Complex IV (EC 1.9.3.1)
    Language English
    Publishing date 2021-05-09
    Publishing country England
    Document type Journal Article
    ZDB-ID 2406833-0
    ISSN 1755-0998 ; 1755-098X
    ISSN (online) 1755-0998
    ISSN 1755-098X
    DOI 10.1111/1755-0998.13402
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Genetic landscape clustering of a large DNA barcoding data set reveals shared patterns of genetic divergence among freshwater fishes of the Maroni Basin

    Papa, Yvan / Le Bail, Pierre‐Yves / Covain, Raphaël

    Molecular ecology resources. 2021 Aug., v. 21, no. 6

    2021  

    Abstract: The Maroni is one of the most speciose basins of the Guianas and hosts a megadiverse freshwater fish community. Although taxonomic references based on morphological identification exist for both the Surinamese and Guianese parts of the basin, there are ... ...

    Abstract The Maroni is one of the most speciose basins of the Guianas and hosts a megadiverse freshwater fish community. Although taxonomic references based on morphological identification exist for both the Surinamese and Guianese parts of the basin, there are still taxonomic uncertainties concerning the status of several species. We used COI sequences of 1284 fish in conjunction with morphological and biogeographical evidence to assist with species delineation and discovery in order to validate and standardize the current taxonomy. This resulted in a final DNA barcode data set of 199 fish species (125 genera, 36 families and eight orders; 68.86% of strictly freshwater fishes from the basin), among which 25 are new putative candidate species flagged as requiring taxonomic update. DNA barcoding delineation through Barcode Index Numbers (BINs) revealed further cryptic diversity (230 BINs in total). To explore global genetic patterns across the basin, genetic divergence landscapes were computed for 128 species, showing a global trend of high genetic divergence between the Surinamese southwest (Tapanahony and Paloemeu), the Guianese southeast (Marouini, Litany, Tampok, etc.), and the river outlet in the north. This could be explained by lower levels of connectivity between these three main areas and/or the exchange of individuals between these areas and the neighbouring basins. A new method of ordination of genetic landscapes successfully assigned species into cluster groups based on their respective pattern of genetic divergence across the Maroni Basin: genetically homogeneous species were effectively discriminated from species showing high spatial genetic fragmentation and possible lower capacity for dispersal.
    Keywords DNA barcoding ; basins ; data collection ; fish communities ; freshwater ; freshwater fish ; genetic similarity ; genetic variation ; geographical distribution ; landscape genetics ; rivers ; taxonomy
    Language English
    Dates of publication 2021-08
    Size p. 2109-2124.
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 2406816-0
    ISSN 1471-8286 ; 1755-098X
    ISSN (online) 1471-8286
    ISSN 1755-098X
    DOI 10.1111/1755-0998.13402
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  3. Article ; Online: Environmental DNA reveals a mismatch between diversity facets of Amazonian fishes in response to contrasting geographical, environmental and anthropogenic effects

    Coutant, Opale / Jézéquel, Céline / Mokany, Karel / Cantera, Isabel / Covain, Raphaël / Valentini, Alice / Dejean, Tony / Brosse, Sébastien / Murienne, Jérôme

    Global Change Biology. 2023 Apr., v. 29, no. 7 p.1741-1758

    2023  

    Abstract: Freshwater ecosystems are among the most endangered ecosystem in the world. Understanding how human activities affect these ecosystems requires disentangling and quantifying the contribution of the factors driving community assembly. While it has been ... ...

    Abstract Freshwater ecosystems are among the most endangered ecosystem in the world. Understanding how human activities affect these ecosystems requires disentangling and quantifying the contribution of the factors driving community assembly. While it has been largely studied in temperate freshwaters, tropical ecosystems remain challenging to study due to the high species richness and the lack of knowledge on species distribution. Here, the use of eDNA‐based fish inventories combined to a community‐level modelling approach allowed depicting of assembly rules and quantifying the relative contribution of geographic, environmental and anthropic factors to fish assembly. We then used the model predictions to map spatial biodiversity and assess the representativity of sites surveyed in French Guiana within the EU Water Framework Directive (WFD) and highlighted areas that should host unique freshwater fish assemblages. We demonstrated a mismatch between the taxonomic and functional diversity. Taxonomic assemblages between but also within basins were mainly the results of dispersal limitation resulting from basin isolation and natural river barriers. Contrastingly, functional assemblages were ruled by environmental and anthropic factors. The regional mapping of fish diversity indicated that the sites surveyed within the EU WFD had a better representativity of the regional functional diversity than taxonomic diversity. Importantly, we also showed that the assemblages expected to be the most altered by anthropic factors were the most poorly represented in terms of functional diversity in the surveyed sites. The predictions of unique functional and taxonomic assemblages could, therefore, guide the establishment of new survey sites to increase fish diversity representativity and improve this monitoring program.
    Keywords basins ; endangered ecosystems ; environmental DNA ; freshwater ; freshwater fish ; functional diversity ; geographical distribution ; global change ; humans ; rivers ; species richness ; surveys ; French Guiana
    Language English
    Dates of publication 2023-04
    Size p. 1741-1758.
    Publishing place John Wiley & Sons, Ltd
    Document type Article ; Online
    Note JOURNAL ARTICLE
    ZDB-ID 1281439-8
    ISSN 1365-2486 ; 1354-1013
    ISSN (online) 1365-2486
    ISSN 1354-1013
    DOI 10.1111/gcb.16533
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  4. Article ; Online: Biogeography of the freshwater fishes of the Guianas using a partitioned parsimony analysis of endemicity with reappraisal of ecoregional boundaries.

    Lemopoulos, Alexandre / Covain, Raphaël

    Cladistics : the international journal of the Willi Hennig Society

    2018  Volume 35, Issue 1, Page(s) 106–124

    Abstract: The Guianas are one of the most diverse regions of the Neotropics, hosting a particularly high rate of freshwater fish endemism. The present distributional patterns of freshwater fish species in the major catchments of the Guianas (comprising Guyana, ... ...

    Abstract The Guianas are one of the most diverse regions of the Neotropics, hosting a particularly high rate of freshwater fish endemism. The present distributional patterns of freshwater fish species in the major catchments of the Guianas (comprising Guyana, Suriname and French Guiana) were analysed to reveal the faunal relationships between rivers, evaluate different hypotheses concerning biogeographical units, and redefine the boundaries of the Guianese freshwater ecoregions. A parsimony analysis of endemicity was performed using a data partitioning strategy to alleviate some drawbacks inherent to the method (e.g. long branch attraction artefact, heterotachy), and take into account alternative parsimony models assigning different constraints on state changes for the different species. A strong spatial element was present in the data with a structuring of species along a west-east gradient. Two main biogeographical units were highlighted: one to the west, ranging from the Essequibo to the Commewijne rivers and including the Proto Berbice and Surinamese regions, and one to the east ranging from the Maroni to the Oyapock rivers and including the Western, Central and Eastern French Guiana regions. Each ecoregion possessed distinctive fish assemblages, and three to four potential zones of faunal exchanges between Amazonian and Guianese rivers have been confirmed.
    Language English
    Publishing date 2018-07-02
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1462608-1
    ISSN 1096-0031 ; 0748-3007
    ISSN (online) 1096-0031
    ISSN 0748-3007
    DOI 10.1111/cla.12341
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: A new species of mailed catfish of genus Rhadinoloricaria (Siluriformes: Loricariidae: Loricariinae) from Rio Negro basin, Brazil

    Crispim‐Rodrigues, Jefferson L. / Bernt, Maxwell J. / Waltz, Brandon T. / Silva, Gabriel S. C. / Benine, Ricardo C. / Oliveira, Cláudio / Covain, Raphaël / Roxo, Fábio F.

    Journal of Fish Biology. 2023 July, v. 103, no. 1 p.103-112

    2023  

    Abstract: During a recent collection expedition to the Rio Negro, in the state of Amazonas, Brazil, eight individuals of an unknown species were collected, with a combination of characteristics that placed the species in the genus Rhadinoloricaria. Furthermore, ... ...

    Abstract During a recent collection expedition to the Rio Negro, in the state of Amazonas, Brazil, eight individuals of an unknown species were collected, with a combination of characteristics that placed the species in the genus Rhadinoloricaria. Furthermore, the presence of two autapomorphic characteristics, including numerous elongated papillae on the lower lip and unbranched barbelets on the margin of lower lip, suggests that it is a new species. From morphological and phylogenetic analyses, including the sequencing of specific genes to calculate the maximum likelihood analyses, coupled with osteological computed tomography (CT) scan analyses, the authors corroborated that the specimens represent a new species of Rhadinoloricaria, described in the present study.
    Keywords Loricariidae ; basins ; catfish ; computed tomography ; new species ; phylogeny ; statistical analysis ; Brazil
    Language English
    Dates of publication 2023-07
    Size p. 103-112.
    Publishing place Blackwell Publishing Ltd
    Document type Article ; Online
    Note JOURNAL ARTICLE
    ZDB-ID 410564-3
    ISSN 1095-8649 ; 0022-1112
    ISSN (online) 1095-8649
    ISSN 0022-1112
    DOI 10.1111/jfb.15402
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  6. Article ; Online: An integrative framework to reevaluate the Neotropical catfish genus Guyanancistrus (Siluriformes: Loricariidae) with particular emphasis on the Guyanancistrus brevispinis complex.

    Fisch-Muller, Sonia / Mol, Jan H A / Covain, Raphaël

    PloS one

    2018  Volume 13, Issue 1, Page(s) e0189789

    Abstract: Characterizing and naming species becomes more and more challenging due to the increasing difficulty of accurately delineating specific bounderies. In this context, integrative taxonomy aims to delimit taxonomic units by leveraging the complementarity of ...

    Abstract Characterizing and naming species becomes more and more challenging due to the increasing difficulty of accurately delineating specific bounderies. In this context, integrative taxonomy aims to delimit taxonomic units by leveraging the complementarity of multiple data sources (geography, morphology, genetics, etc.). However, while the theoretical framework of integrative taxonomy has been explicitly stated, methods for the simultaneous analysis of multiple data sets are poorly developed and in many cases different information sources are still explored successively. Multi-table methods developed in the field of community ecology provide such an intregrative framework. In particular, multiple co-inertia analysis is flexible enough to allow the integration of morphological, distributional, and genetic data in the same analysis. We have applied this powerfull approach to delimit species boundaries in a group of poorly differentiated catfishes belonging to the genus Guyanancistrus from the Guianas region of northeastern South America. Because the species G. brevispinis has been claimed to be a species complex consisting of five species, particular attention was paid to taxon. Separate analyses indicated the presence of eight distinct species of Guyanancistrus, including five new species and one new genus. However, none of the preliminary analyses revealed different lineages within G. brevispinis, and the multi-table analysis revealed three intraspecific lineages. After taxonomic clarifications and description of the new genus, species and subspecies, a reappraisal of the biogeography of Guyanancistrus members was performed. This analysis revealed three distinct dispersals from the Upper reaches of Amazonian tributaries toward coastal rivers of the Eastern Guianas Ecoregion. The central role played by the Maroni River, as gateway from the Amazon basin, was confirmed. The Maroni River was also found to be a center of speciation for Guyanancistrus (with three species and two subspecies), as well as a source of dispersal of G. brevispinis toward the other main basins of the Eastern Guianas.
    MeSH term(s) Animals ; Catfishes/classification ; Phylogeography ; Rivers ; South America ; Tropical Climate
    Language English
    Publishing date 2018
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1932-6203
    ISSN (online) 1932-6203
    DOI 10.1371/journal.pone.0189789
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Environmental DNA reveals a mismatch between diversity facets of Amazonian fishes in response to contrasting geographical, environmental and anthropogenic effects.

    Coutant, Opale / Jézéquel, Céline / Mokany, Karel / Cantera, Isabel / Covain, Raphaël / Valentini, Alice / Dejean, Tony / Brosse, Sébastien / Murienne, Jérôme

    Global change biology

    2022  Volume 29, Issue 7, Page(s) 1741–1758

    Abstract: Freshwater ecosystems are among the most endangered ecosystem in the world. Understanding how human activities affect these ecosystems requires disentangling and quantifying the contribution of the factors driving community assembly. While it has been ... ...

    Abstract Freshwater ecosystems are among the most endangered ecosystem in the world. Understanding how human activities affect these ecosystems requires disentangling and quantifying the contribution of the factors driving community assembly. While it has been largely studied in temperate freshwaters, tropical ecosystems remain challenging to study due to the high species richness and the lack of knowledge on species distribution. Here, the use of eDNA-based fish inventories combined to a community-level modelling approach allowed depicting of assembly rules and quantifying the relative contribution of geographic, environmental and anthropic factors to fish assembly. We then used the model predictions to map spatial biodiversity and assess the representativity of sites surveyed in French Guiana within the EU Water Framework Directive (WFD) and highlighted areas that should host unique freshwater fish assemblages. We demonstrated a mismatch between the taxonomic and functional diversity. Taxonomic assemblages between but also within basins were mainly the results of dispersal limitation resulting from basin isolation and natural river barriers. Contrastingly, functional assemblages were ruled by environmental and anthropic factors. The regional mapping of fish diversity indicated that the sites surveyed within the EU WFD had a better representativity of the regional functional diversity than taxonomic diversity. Importantly, we also showed that the assemblages expected to be the most altered by anthropic factors were the most poorly represented in terms of functional diversity in the surveyed sites. The predictions of unique functional and taxonomic assemblages could, therefore, guide the establishment of new survey sites to increase fish diversity representativity and improve this monitoring program.
    MeSH term(s) Animals ; Humans ; Ecosystem ; DNA, Environmental ; Anthropogenic Effects ; Biodiversity ; Fishes/physiology ; Environmental Monitoring
    Chemical Substances DNA, Environmental
    Language English
    Publishing date 2022-11-27
    Publishing country England
    Document type Journal Article
    ZDB-ID 1281439-8
    ISSN 1365-2486 ; 1354-1013
    ISSN (online) 1365-2486
    ISSN 1354-1013
    DOI 10.1111/gcb.16533
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  8. Article: Fish communities critically depend on forest subsidies in small neotropical streams with high biodiversity value

    Roussel, Jean‐Marc / Covain, Raphael / Vigouroux, Regis / Allard, Luc / Treguier, Anne / Papa, Yvan / Le Bail, Pierre‐Yves

    Biotropica. 2021 July, v. 53, no. 4

    2021  

    Abstract: Trophic support of species‐rich tropical fish communities remains uncertain in small forest streams where dense and constant canopy cover strongly limits the solar radiation available to aquatic producers. We sampled >1300 fish from 80 species in 14 ... ...

    Abstract Trophic support of species‐rich tropical fish communities remains uncertain in small forest streams where dense and constant canopy cover strongly limits the solar radiation available to aquatic producers. We sampled >1300 fish from 80 species in 14 remote headwater streams during the dry season in French Guiana. Carbon and nitrogen stable isotope analyses and mixing models were run to estimate the trophic position (TP) of each species and its dependence on terrestrial organic carbon sources. Matching patterns were found between nitrogen stable isotope ratios and the literature on diets of species at the base and top of the food web. Nearly 60% of the species fed exclusively on animal prey (mean TP >3), including piscivorous and parasitic fish species. Phylogenetic analysis revealed that TP were distributed broadly along the phylogeny and changed gradually within lineages. Carbon stable isotope ratios differed strongly between herbivores that fed on aquatic primary producers and those that fed on terrestrial plants along banksides. Terrestrial carbon was dominant in nearly 90% of species, and most of them depended more or less exclusively on terrestrial carbon. Ancestral state reconstructions indicated that only a few lineages showed local changes toward the consumption of algal carbon. We conclude that fish in neotropical headwater streams shaded by a dense tree canopy depend strongly on terrestrial subsidies. Changes in this aquatic‐terrestrial linkage would have major consequences for the remarkable fish biodiversity in these streams. Abstract in French is available with online material.
    Keywords Neotropics ; algae ; biodiversity ; canopy ; dry season ; food webs ; forests ; nitrogen ; organic carbon ; phylogeny ; piscivores ; solar radiation ; stable isotopes ; trees ; tropical fish ; water ; French Guiana
    Language English
    Dates of publication 2021-07
    Size p. 1096-1108.
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 2052061-X
    ISSN 1744-7429 ; 0006-3606
    ISSN (online) 1744-7429
    ISSN 0006-3606
    DOI 10.1111/btp.12949
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  9. Article ; Online: A new species of mailed catfish of genus Rhadinoloricaria (Siluriformes: Loricariidae: Loricariinae) from Rio Negro basin, Brazil.

    Crispim-Rodrigues, Jefferson L / Bernt, Maxwell J / Waltz, Brandon T / Silva, Gabriel S C / Benine, Ricardo C / Oliveira, Claudio / Covain, Raphaël / Roxo, Fábio F

    Journal of fish biology

    2023  Volume 103, Issue 1, Page(s) 103–112

    Abstract: During a recent collection expedition to the Rio Negro, in the state of Amazonas, Brazil, eight individuals of an unknown species were collected, with a combination of characteristics that placed the species in the genus Rhadinoloricaria. Furthermore, ... ...

    Abstract During a recent collection expedition to the Rio Negro, in the state of Amazonas, Brazil, eight individuals of an unknown species were collected, with a combination of characteristics that placed the species in the genus Rhadinoloricaria. Furthermore, the presence of two autapomorphic characteristics, including numerous elongated papillae on the lower lip and unbranched barbelets on the margin of lower lip, suggests that it is a new species. From morphological and phylogenetic analyses, including the sequencing of specific genes to calculate the maximum likelihood analyses, coupled with osteological computed tomography (CT) scan analyses, the authors corroborated that the specimens represent a new species of Rhadinoloricaria, described in the present study.
    MeSH term(s) Animals ; Brazil ; Catfishes/anatomy & histology ; Catfishes/genetics ; Phylogeny ; Tomography, X-Ray Computed ; Skeleton/diagnostic imaging
    Language English
    Publishing date 2023-05-11
    Publishing country England
    Document type Journal Article
    ZDB-ID 410564-3
    ISSN 1095-8649 ; 0022-1112
    ISSN (online) 1095-8649
    ISSN 0022-1112
    DOI 10.1111/jfb.15402
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  10. Article ; Online: Complete mitochondrial genome of the river stingray

    Ory, David / Cuenot, Yves / Vigouroux, Régis / Covain, Raphaël / Brosse, Sébastien / Murienne, Jérôme

    Mitochondrial DNA. Part B, Resources

    2019  Volume 4, Issue 2, Page(s) 3153–3154

    Abstract: The river ... ...

    Abstract The river stingray
    Language English
    Publishing date 2019-09-20
    Publishing country England
    Document type Journal Article
    ISSN 2380-2359
    ISSN (online) 2380-2359
    DOI 10.1080/23802359.2019.1666683
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