Article ; Online: Triazole-derivatized near-infrared cyanine dyes enable local functional fluorescent imaging of ocular inflammation.
2022 Volume 216, Page(s) 114623
Abstract: Near-infrared (NIR) chemical fluorophores are promising tools for in-vivo imaging in real time but often succumb to rapid photodegradation. Indocyanine green (ICG) is the only NIR dye with regulatory approval for ocular imaging in humans; however, ICG, ... ...
| Abstract | Near-infrared (NIR) chemical fluorophores are promising tools for in-vivo imaging in real time but often succumb to rapid photodegradation. Indocyanine green (ICG) is the only NIR dye with regulatory approval for ocular imaging in humans; however, ICG, when employed for applications such as labelling immune cells, has limited sensitivity and does not allow precise detection of specific inflammatory events, for example leukocyte recruitment during uveitic flare-ups. We investigated the potential use of photostable novel triazole NIR cyanine (TNC) dyes for detecting and characterising activated T-cell activity within the eye. Three TNC dyes were evaluated for ocular cytotoxicity in-vitro using a MTT assay and optimised concentrations for intraocular detection within ex-vivo porcine eyes after topical application or intracameral injections of the dyes. TNC labelled T-cell tracking experiments and mechanistic studies were also performed in-vitro. TNC-1 and TNC-2 dyes exhibited greater fluorescence intensity than ICG at 10 μM, whereas TNC-3 was only detectable at 100 μM within the porcine eye. TNC dyes did not demonstrate any ocular cell toxicity at working concentrations of 10 μM. CD4 |
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| MeSH term(s) | Animals ; Biosensing Techniques ; Fluorescent Dyes/metabolism ; Humans ; Indocyanine Green/metabolism ; Inflammation/chemically induced ; Optical Imaging/methods ; Swine ; Triazoles |
| Chemical Substances | Fluorescent Dyes ; Triazoles ; Indocyanine Green (IX6J1063HV) |
| Language | English |
| Publishing date | 2022-08-13 |
| Publishing country | England |
| Document type | Journal Article |
| ZDB-ID | 1011023-9 |
| ISSN | 1873-4235 ; 0956-5663 |
| ISSN (online) | 1873-4235 |
| ISSN | 0956-5663 |
| DOI | 10.1016/j.bios.2022.114623 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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