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  1. Article ; Online: A portable NIR fluorimeter directly quantifies singlet oxygen generated by nanostructures for Photodynamic Therapy.

    Orsi, Davide / Vaccari, Marco / Baraldi, Andrea / Cristofolini, Luigi

    Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

    2021  Volume 265, Page(s) 120357

    Abstract: This paper reports on the setting up and calibration of a portable NIR fluorimeter specifically developed for quantitative direct detection of the highly reactive singlet oxygen ( ...

    Abstract This paper reports on the setting up and calibration of a portable NIR fluorimeter specifically developed for quantitative direct detection of the highly reactive singlet oxygen (
    MeSH term(s) Infrared Rays ; Nanostructures ; Photochemotherapy ; Photosensitizing Agents ; Reactive Oxygen Species ; Singlet Oxygen
    Chemical Substances Photosensitizing Agents ; Reactive Oxygen Species ; Singlet Oxygen (17778-80-2)
    Language English
    Publishing date 2021-09-04
    Publishing country England
    Document type Journal Article
    ZDB-ID 210413-1
    ISSN 1873-3557 ; 0370-8322 ; 0584-8539 ; 1386-1425
    ISSN (online) 1873-3557
    ISSN 0370-8322 ; 0584-8539 ; 1386-1425
    DOI 10.1016/j.saa.2021.120357
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  2. Article: Synchrotron X-ray techniques for the investigation of structures and dynamics in interfacial systems

    Cristofolini, Luigi

    Current opinion in colloid & interface science. 2014 June, v. 19, no. 3

    2014  

    Abstract: This review focuses on recent results obtained by synchrotron X-ray techniques applied to the characterization of interfacial systems, with main emphasis on flat interfaces and on colloidal systems. The techniques covered are, for structural ... ...

    Abstract This review focuses on recent results obtained by synchrotron X-ray techniques applied to the characterization of interfacial systems, with main emphasis on flat interfaces and on colloidal systems. The techniques covered are, for structural determinations: X-ray reflectivity (XRR), grazing incidence X-ray diffraction (GIXRD) and grazing incidence X-ray excited fluorescence (GIXF), while dynamics are investigated by X-ray photon correlation spectroscopy (XPCS) mainly in the grazing-incidence geometry (GIXPCS).The systems reviewed are, in order of growing complexity, floating Langmuir monolayers, supported films of lipids and proteins, polymeric films, buried interfaces, colloidal systems and gels formed by colloids either in 3D or in the form of 2D interfacial layers. Recent results are critically discussed, and some interesting directions of development are outlined, having also in mind new technical developments such as X-ray free electron laser sources and micro-focused synchrotron beamlines.
    Keywords colloids ; fluorescence ; gels ; lipids ; photons ; proteins ; spectroscopy ; X-radiation ; X-ray diffraction ; X-ray reflectivity
    Language English
    Dates of publication 2014-06
    Size p. 228-241.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 2019683-0
    ISSN 1359-0294
    ISSN 1359-0294
    DOI 10.1016/j.cocis.2014.03.006
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: Chemico-Physical Properties of Some 1,1'-Bis-alkyl-2,2'-hexane-1,6-diyl-bispyridinium Chlorides Hydrogenated and Partially Fluorinated for Gene Delivery.

    Massa, Michele / Rivara, Mirko / Pertinhez, Thelma A / Compari, Carlotta / Donofrio, Gaetano / Cristofolini, Luigi / Orsi, Davide / Franceschi, Valentina / Fisicaro, Emilia

    Molecules (Basel, Switzerland)

    2023  Volume 28, Issue 8

    Abstract: The development of very efficient and safe non-viral vectors, constituted mainly by cationic lipids bearing multiple charges, is a landmark for in vivo gene-based medicine. To understand the effect of the hydrophobic chain's length, we here report the ... ...

    Abstract The development of very efficient and safe non-viral vectors, constituted mainly by cationic lipids bearing multiple charges, is a landmark for in vivo gene-based medicine. To understand the effect of the hydrophobic chain's length, we here report the synthesis, and the chemico-physical and biological characterization, of a new term of the homologous series of hydrogenated
    MeSH term(s) Chlorides ; Hexanes ; Gene Transfer Techniques ; Surface-Active Agents/chemistry
    Chemical Substances Chlorides ; n-hexane (2DDG612ED8) ; Hexanes ; Surface-Active Agents
    Language English
    Publishing date 2023-04-20
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1413402-0
    ISSN 1420-3049 ; 1431-5165 ; 1420-3049
    ISSN (online) 1420-3049
    ISSN 1431-5165 ; 1420-3049
    DOI 10.3390/molecules28083585
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    Zs.MO 81: Show issues

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  4. Article ; Online: Emulsification and emulsion stability: The role of the interfacial properties.

    Ravera, Francesca / Dziza, Katarzyna / Santini, Eva / Cristofolini, Luigi / Liggieri, Libero

    Advances in colloid and interface science

    2020  Volume 288, Page(s) 102344

    Abstract: In this review, we highlight and discuss the effects of interfacial properties on the major mechanisms governing the aging of emulsions: flocculation, coalescence and Ostwald ripening. The process of emulsification is also addressed, as it is well ... ...

    Abstract In this review, we highlight and discuss the effects of interfacial properties on the major mechanisms governing the aging of emulsions: flocculation, coalescence and Ostwald ripening. The process of emulsification is also addressed, as it is well recognized that the adsorption properties of emulsifiers play an important role on it. The consolidated background on these phenomena is briefly summarised based on selected literature, reporting relevant findings and results, and discussing some criticalities. The typical experimental approaches adopted to investigate the above effects are also summarised, underlining in particular the role of adsorption at the droplet interface. Attention is paid to different types of surface-active species involved with emulsion production, including solid particles. The latter being of increasing interest in a wide variety of emulsions-related products and technologies in various fields. The possibility to stop the long term aging caused by Ostwald ripening in emulsions is also discussed, quantifying under which conditions it may occur in practice.
    Language English
    Publishing date 2020-12-14
    Publishing country Netherlands
    Document type Journal Article ; Review
    ZDB-ID 210507-x
    ISSN 1873-3727 ; 0001-8686
    ISSN (online) 1873-3727
    ISSN 0001-8686
    DOI 10.1016/j.cis.2020.102344
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  5. Article: Characterization of the dynamics of interfaces and of interface-dominated systems via spectroscopy and microscopy techniques

    Cristofolini, Luigi / Isa, Lucio / Orsi, Davide

    Current opinion in colloid & interface science. 2018 Sept., v. 37

    2018  

    Abstract: Complex interfaces, such as vesicles and biological membranes, and interface-dominated systems, such as foams and emulsions, are central to a vast range of naturally occurring and synthetic materials. In order to characterize their dynamics, it is ... ...

    Abstract Complex interfaces, such as vesicles and biological membranes, and interface-dominated systems, such as foams and emulsions, are central to a vast range of naturally occurring and synthetic materials. In order to characterize their dynamics, it is necessary to cover a wide range of length and time scales. This can only be done by a combination of different techniques. We review here recent results and discuss new trends focusing on two class of techniques: correlation spectroscopies and microscopies, highlighting some examples of their combined use.
    Keywords emulsions ; foams ; microscopy ; spectroscopy ; synthetic products
    Language English
    Dates of publication 2018-09
    Size p. 13-32.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 2019683-0
    ISSN 1359-0294
    ISSN 1359-0294
    DOI 10.1016/j.cocis.2018.06.001
    Database NAL-Catalogue (AGRICOLA)

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  6. Article ; Online: Gene-Delivery Ability of New Hydrogenated and Partially Fluorinated

    Massa, Michele / Rivara, Mirko / Donofrio, Gaetano / Cristofolini, Luigi / Peracchia, Erica / Compari, Carlotta / Bacciottini, Franco / Orsi, Davide / Franceschi, Valentina / Fisicaro, Emilia

    International journal of molecular sciences

    2022  Volume 23, Issue 6

    Abstract: The pandemic emergency determined by the spreading worldwide of the SARS-CoV-2 virus has focused the scientific and economic efforts of the pharmaceutical industry and governments on the possibility to fight the virus by genetic immunization. The genetic ...

    Abstract The pandemic emergency determined by the spreading worldwide of the SARS-CoV-2 virus has focused the scientific and economic efforts of the pharmaceutical industry and governments on the possibility to fight the virus by genetic immunization. The genetic material must be delivered inside the cells by means of vectors. Due to the risk of adverse or immunogenic reaction or replication connected with the more efficient viral vectors, non-viral vectors are in many cases considered as a preferred strategy for gene delivery into eukaryotic cells. This paper is devoted to the evaluation of the gene delivery ability of new synthesized
    MeSH term(s) A549 Cells ; Cell Survival ; DNA/chemistry ; DNA/genetics ; DNA/metabolism ; Gene Transfer Techniques ; Genetic Therapy/methods ; Halogenation ; Humans ; Hydrogenation ; Methane/chemistry ; Methane/metabolism ; Microscopy, Atomic Force ; Molecular Structure ; Plasmids/chemistry ; Plasmids/genetics ; Plasmids/metabolism ; Pyridinium Compounds/chemistry ; Pyridinium Compounds/metabolism ; Reproducibility of Results ; Surface-Active Agents/chemistry ; Surface-Active Agents/metabolism ; Transfection/methods
    Chemical Substances Pyridinium Compounds ; Surface-Active Agents ; DNA (9007-49-2) ; Methane (OP0UW79H66)
    Language English
    Publishing date 2022-03-11
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms23063062
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  7. Article ; Online: Soft pinning of liquid domains on topographical hemispherical caps.

    Feriani, Luigi / Cristofolini, Luigi / Cicuta, Pietro

    Chemistry and physics of lipids

    2015  Volume 185, Page(s) 78–87

    Abstract: The role of lipid composition as a regulator or mediator of processes that take place in biological membranes is a very topical question, and important insights can be gained by studying in vitro model lipid mixture systems. A particular question is the ... ...

    Abstract The role of lipid composition as a regulator or mediator of processes that take place in biological membranes is a very topical question, and important insights can be gained by studying in vitro model lipid mixture systems. A particular question is the coupling of local curvature to the local phases in membranes of mixed composition. Working with an experimental system of giant unilamellar vesicles of ternary composition, the curvature is imposed by approaching the membrane to a topographically (on the micron scale) patterned surface. Performing experiments, we show that domains of the more disordered phase localise preferentially to regions of higher curvature. We characterise and discuss the strength of this "caging" behaviour. In future, the setup we discuss here could prove useful as a platform to localise domains rich in membrane proteins, or to promote the onset of biochemical processes at specific locations. Finally, we note that the methods developed here could have also applications in bio-sensing, as a similar but metal coated topography can sustain plasmonic resonances.
    MeSH term(s) Biomechanical Phenomena ; Cell Membrane/chemistry ; Dimethylpolysiloxanes/chemistry ; Lipid Bilayers/chemistry ; Models, Molecular ; Surface Properties
    Chemical Substances Dimethylpolysiloxanes ; Lipid Bilayers ; baysilon (63148-62-9)
    Language English
    Publishing date 2015-01
    Publishing country Ireland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 213869-4
    ISSN 1873-2941 ; 0009-3084
    ISSN (online) 1873-2941
    ISSN 0009-3084
    DOI 10.1016/j.chemphyslip.2014.07.012
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    Zs.A 804: Show issues Location:
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  8. Article ; Online: Recent developments in emulsion characterization: Diffusing Wave Spectroscopy beyond average values.

    Lorusso, Valentina / Orsi, Davide / Salerni, Fabrizia / Liggieri, Libero / Ravera, Francesca / McMillin, Robert / Ferri, James / Cristofolini, Luigi

    Advances in colloid and interface science

    2020  Volume 288, Page(s) 102341

    Abstract: We report here an overview of current trends and a selection of recent results regarding the characterization of emulsions by Diffusing Wave Spectroscopy (DWS). We provide a synopsis of the state of the art of the DWS technique, and a critical discussion ...

    Abstract We report here an overview of current trends and a selection of recent results regarding the characterization of emulsions by Diffusing Wave Spectroscopy (DWS). We provide a synopsis of the state of the art of the DWS technique, and a critical discussion of experiments performed on samples in which Brownian and ballistic dynamics coexist. A novel analysis scheme is introduced for DWS experiments on creaming or sedimenting emulsions, allowing to extract not only average values for drop size and drop dynamics - as usual in DWS - but also properties related to the width of the distributions governing these quantities. This analysis scheme starts from a realistic Monte Carlo simulation of light diffusing in the volume of the sample and reaching the detector. This simulation is more accurate than the analytical expressions available for the idealized geometries normally used in DWS interpretation. By disentangling Brownian and ballistic motions we directly access the variance of velocity distribution, σ
    Language English
    Publishing date 2020-12-13
    Publishing country Netherlands
    Document type Journal Article ; Review
    ZDB-ID 210507-x
    ISSN 1873-3727 ; 0001-8686
    ISSN (online) 1873-3727
    ISSN 0001-8686
    DOI 10.1016/j.cis.2020.102341
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  9. Article ; Online: Magnetically responsive polycaprolactone nanocarriers for application in the biomedical field: magnetic hyperthermia, magnetic resonance imaging, and magnetic drug delivery.

    Szczęch, Marta / Orsi, Davide / Łopuszyńska, Natalia / Cristofolini, Luigi / Jasiński, Krzysztof / Węglarz, Władysław P / Albertini, Franca / Kereïche, Sami / Szczepanowicz, Krzysztof

    RSC advances

    2020  Volume 10, Issue 71, Page(s) 43607–43618

    Abstract: There are huge demands on multifunctional nanocarriers to be used in nanomedicine. Herein, we present a simple and efficient method for the preparation of multifunctional magnetically responsive polymeric-based nanocarriers optimized for biomedical ... ...

    Abstract There are huge demands on multifunctional nanocarriers to be used in nanomedicine. Herein, we present a simple and efficient method for the preparation of multifunctional magnetically responsive polymeric-based nanocarriers optimized for biomedical applications. The hybrid delivery system is composed of drug-loaded polymer nanoparticles (poly(caprolactone), PCL) coated with a multilayer shell of polyglutamic acid (PGA) and superparamagnetic iron oxide nanoparticles (SPIONs), which are known as bio-acceptable components. The PCL nanocarriers with a model anticancer drug (Paclitaxel, PTX) were formed by the spontaneous emulsification solvent evaporation (SESE) method, while the magnetically responsive multilayer shell was formed
    Language English
    Publishing date 2020-12-08
    Publishing country England
    Document type Journal Article
    ISSN 2046-2069
    ISSN (online) 2046-2069
    DOI 10.1039/d0ra07507h
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  10. Article ; Online: Adsorption of Sodium Dodecyl Sulfate at Water-Dodecane Interface in Relation to the Oil in Water Emulsion Properties.

    Llamas, Sara / Santini, Eva / Liggieri, Libero / Salerni, Fabrizia / Orsi, Davide / Cristofolini, Luigi / Ravera, Francesca

    Langmuir : the ACS journal of surfaces and colloids

    2018  Volume 34, Issue 21, Page(s) 5978–5989

    Abstract: The control of the behavior of oil in water emulsions requires deeper investigations on the adsorption properties of the emulsion stabilizers at the interfaces, which are fundamental to explain the (de)stabilization mechanisms. In this work, we present ... ...

    Abstract The control of the behavior of oil in water emulsions requires deeper investigations on the adsorption properties of the emulsion stabilizers at the interfaces, which are fundamental to explain the (de)stabilization mechanisms. In this work, we present an extensive study on oil-in-water emulsions stabilized by sodium dodecyl sulfate (SDS) below its critical micellar concentration. Dynamic tensiometry, dilational rheology, and electrical conductivity measurements are used to investigate the adsorption properties at the droplet interface, whereas the aging of the respective emulsions was investigated by monitoring the macroscopic thickness of the emulsion layer, by microimaging and dynamic light scattering (DLS) analysis, to get information on the drop size distribution. In addition, the droplet coalescence is investigated by a microscopy setup. The results of this multitechnique study allow deriving a coherent scenario where the adsorption properties of this ionic surfactant relate to those of the emulsion, such as, for example, the prevention of droplet coalescence and the presence of other mechanisms, such as Ostwald ripening, responsible for the emulsion aging.
    Language English
    Publishing date 2018-05-14
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2005937-1
    ISSN 1520-5827 ; 0743-7463
    ISSN (online) 1520-5827
    ISSN 0743-7463
    DOI 10.1021/acs.langmuir.8b00358
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