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Article: SARS-CoV-2: theoretical analysis of the proposed algorithms to the enhancement and segmentation of high-resolution microscopy images-Part II.

Rodríguez, Roberto / Mondeja, Brian A / Valdes, Odalys / Resik, Sonia / Vizcaino, Ananayla / Acosta, Emilio F / González, Yorexis / Kourí, Vivian / Díaz, Angelina / Guzmán, María G

Signal, image and video processing

2022 Volume 16, Issue 3, Page(s) 595–604

Abstract: Today is a reality that the novel coronavirus SARS-Cov-2 has become a global pandemic. For this reason, the study of real microscopic images of this coronavirus is of great importance, as it allows us to carry out a more precise research on it. However, ... ...

Abstract	Today is a reality that the novel coronavirus SARS-Cov-2 has become a global pandemic. For this reason, the study of real microscopic images of this coronavirus is of great importance, as it allows us to carry out a more precise research on it. However, as we pointed out in a former paper as reported by Roberto Rodríguez (SARS-CoV-2: Enhancement and Segmentation of High-Resolution Microscopy Images. Part I", Sent to Signal, Image and Video Processing Video Processing, Springer, New York, 2020), many times these microscopic images present some blurring problems, which are always susceptible to be improved. The aim of this work is to carry out a theoretical analysis of the proposed algorithms to enhancement and segmentation of these microscopic images, which is important for the design and development of future algorithms before new epidemics.
Language	English
Publishing date	2022-01-12
Publishing country	England
Document type	Journal Article
ZDB-ID	2391619-9
ISSN	1863-1711 ; 1863-1703
ISSN (online)	1863-1711
ISSN	1863-1703
DOI	10.1007/s11760-021-02045-7
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Article: SARS-CoV-2: enhancement and segmentation of high-resolution microscopy images-Part I.

Rodríguez, Roberto / Mondeja, Brian A / Valdés, Odalys / Resik, Sonia / Vizcaino, Ananayla / Acosta, Emilio F / González, Yorexis / Kourí, Vivian / Díaz, Angelina / Guzmán, María G

Signal, image and video processing

2021 Volume 15, Issue 8, Page(s) 1713–1721

Abstract: Possibly, and due to poor eating habits and unhealthy lifestyle, many viruses are transmitted to human people. Such is the case, of the novel coronavirus SARS-Cov-2, which has expanded of exponential way, practically, to whole world population. For this ... ...

Abstract	Possibly, and due to poor eating habits and unhealthy lifestyle, many viruses are transmitted to human people. Such is the case, of the novel coronavirus SARS-Cov-2, which has expanded of exponential way, practically, to whole world population. For this reason, the enhancement of real microscopic images of this coronavirus is of great importance. Of this way, one can highlight the
Language	English
Publishing date	2021-04-23
Publishing country	England
Document type	Journal Article
ZDB-ID	2391619-9
ISSN	1863-1711 ; 1863-1703
ISSN (online)	1863-1711
ISSN	1863-1703
DOI	10.1007/s11760-021-01912-7
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Article: SARS-CoV-2: preliminary study of infected human nasopharyngeal tissue by high resolution microscopy

Mondeja, Brian / Valdes, Odalys / Resik, Sonia / Vizcaino, Ananayla / Acosta, Emilio / Montalván, Adelmo / Paez, Amira / Mune, Mayra / Rodríguez, Roberto / Valdés, Juan / Gonzalez, Guelsys / Sanchez, Daisy / Falcón, Viviana / González, Yorexis / Kourí, Vivian / Díaz, Angelina / Guzmán, María

Virology journal. 2021 Dec., v. 18, no. 1

2021

Abstract: BACKGROUND: The novel coronavirus SARS-CoV-2 is the etiological agent of COVID-19. This virus has become one of the most dangerous in recent times with a very high rate of transmission. At present, several publications show the typical crown-shape of the ...

Institution	The IPK Virology Research Group
Abstract	BACKGROUND: The novel coronavirus SARS-CoV-2 is the etiological agent of COVID-19. This virus has become one of the most dangerous in recent times with a very high rate of transmission. At present, several publications show the typical crown-shape of the novel coronavirus grown in cell cultures. However, an integral ultramicroscopy study done directly from clinical specimens has not been published. METHODS: Nasopharyngeal swabs were collected from 12 Cuban individuals, six asymptomatic and RT-PCR negative (negative control) and six others from a COVID-19 symptomatic and RT-PCR positive for SARS CoV-2. Samples were treated with an aldehyde solution and processed by scanning electron microscopy (SEM), confocal microscopy (CM) and, atomic force microscopy. Improvement and segmentation of coronavirus images were performed by a novel mathematical image enhancement algorithm. RESULTS: The images of the negative control sample showed the characteristic healthy microvilli morphology at the apical region of the nasal epithelial cells. As expected, they do not display virus-like structures. The images of the positive sample showed characteristic coronavirus-like particles and evident destruction of microvilli. In some regions, virions budding through the cell membrane were observed. Microvilli destruction could explain the anosmia reported by some patients. Virus-particles emerging from the cell-surface with a variable size ranging from 80 to 400 nm were observed by SEM. Viral antigen was identified in the apical cells zone by CM. CONCLUSIONS: The integral microscopy study showed that SARS-CoV-2 has a similar image to SARS-CoV. The application of several high-resolution microscopy techniques to nasopharyngeal samples awaits future use.
Keywords	COVID-19 infection ; Severe acute respiratory syndrome coronavirus 2 ; aldehydes ; algorithms ; atomic force microscopy ; confocal microscopy ; epithelium ; etiological agents ; humans ; microvilli ; nose ; viral antigens ; virology ; viruses
Language	English
Dates of publication	2021-12
Size	p. 149.
Publishing place	BioMed Central
Document type	Article
ZDB-ID	2160640-7
ISSN	1743-422X
ISSN	1743-422X
DOI	10.1186/s12985-021-01620-1
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Article: Morphological and molecular characterization supporting Amblyomma mixtum presence in Cuba

Encinosa, Pedro E / García, Yuselys / Lleonart, Ricardo / Aliaga, Daniela / Fernández, Yilian / Bello, Yamil / la Guardia, Carolina de / González, Yorexis / Díaz, Angelina / Estrada, Mario Pablo / Rodríguez-Mallon, Alina

Ticks and tick-borne diseases. 2021 Jan., v. 12, no. 1

2021

Abstract: Amblyomma cajennense Fabricius, 1787 (Acari: Ixodidae) is a widely distributed tick taxon. Recent studies have reassessed this taxon as a complex of six species. Amblyomma mixtum Koch, 1844 has been suggested by some authors as the only species of this ... ...

Abstract	Amblyomma cajennense Fabricius, 1787 (Acari: Ixodidae) is a widely distributed tick taxon. Recent studies have reassessed this taxon as a complex of six species. Amblyomma mixtum Koch, 1844 has been suggested by some authors as the only species of this complex that is present in Cuba. Other authors have pointed a niche overlapping for A. mixtum and A. cajennense s.s. in the country. Detailed taxonomic studies on the Cuban species belonging to this complex are needed in order to evaluate their current distribution according to the recent classification. This study aimed to characterize Cuban populations from the A. cajennense complex by using tick samples obtained from 3 occidental provinces and 1 central province of the country. Morphological identification and measurements of the main relevant taxonomic structures were conducted by using Scanning Electron Microscopy. Phylogenetic analyzes were carried out with 16S ribosomal RNA, internal transcribed spacer 2 and the subunit I of mitochondrial cytochrome c oxidase gene sequences. The results of these studies demonstrated that all samples belonged to the species A. mixtum (Koch, 1844). This study constitutes the first molecular characterization of this Amblyomma species in Cuba. Further studies will be necessary in order to corroborate if A. cajennense s.s. is also present in the island.
Keywords	Amblyomma cajennense ; Amblyomma mixtum ; cytochrome-c oxidase ; internal transcribed spacers ; mitochondria ; phylogeny ; ribosomal DNA ; ribosomal RNA ; scanning electron microscopy ; tick-borne diseases ; ticks ; Cuba
Language	English
Dates of publication	2021-01
Publishing place	Elsevier GmbH
Document type	Article
ZDB-ID	2541872-5
ISSN	1877-9603 ; 1877-959X
ISSN (online)	1877-9603
ISSN	1877-959X
DOI	10.1016/j.ttbdis.2020.101602
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Article ; Online: Morphological and molecular characterization supporting Amblyomma mixtum presence in Cuba.

Ticks and tick-borne diseases

2020 Volume 12, Issue 1, Page(s) 101602

Abstract	Amblyomma cajennense Fabricius, 1787 (Acari: Ixodidae) is a widely distributed tick taxon. Recent studies have reassessed this taxon as a complex of six species. Amblyomma mixtum Koch, 1844 has been suggested by some authors as the only species of this complex that is present in Cuba. Other authors have pointed a niche overlapping for A. mixtum and A. cajennense s.s. in the country. Detailed taxonomic studies on the Cuban species belonging to this complex are needed in order to evaluate their current distribution according to the recent classification. This study aimed to characterize Cuban populations from the A. cajennense complex by using tick samples obtained from 3 occidental provinces and 1 central province of the country. Morphological identification and measurements of the main relevant taxonomic structures were conducted by using Scanning Electron Microscopy. Phylogenetic analyzes were carried out with 16S ribosomal RNA, internal transcribed spacer 2 and the subunit I of mitochondrial cytochrome c oxidase gene sequences. The results of these studies demonstrated that all samples belonged to the species A. mixtum (Koch, 1844). This study constitutes the first molecular characterization of this Amblyomma species in Cuba. Further studies will be necessary in order to corroborate if A. cajennense s.s. is also present in the island.
MeSH term(s)	Amblyomma/anatomy & histology ; Amblyomma/genetics ; Amblyomma/growth & development ; Animal Distribution ; Animals ; Cuba ; DNA, Ribosomal Spacer/analysis ; Dogs/parasitology ; Electron Transport Complex IV/analysis ; Female ; Horses/parasitology ; Larva/anatomy & histology ; Larva/genetics ; Larva/growth & development ; Male ; Nymph/anatomy & histology ; Nymph/genetics ; Nymph/growth & development ; Phylogeny ; RNA, Ribosomal, 16S/analysis ; Sheep, Domestic/parasitology
Chemical Substances	DNA, Ribosomal Spacer ; RNA, Ribosomal, 16S ; Electron Transport Complex IV (EC 1.9.3.1)
Language	English
Publishing date	2020-10-27
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2541872-5
ISSN	1877-9603 ; 1877-959X
ISSN (online)	1877-9603
ISSN	1877-959X
DOI	10.1016/j.ttbdis.2020.101602
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Article ; Online: SARS-CoV-2: preliminary study of infected human nasopharyngeal tissue by high resolution microscopy.

Virology journal

2021 Volume 18, Issue 1, Page(s) 149

Abstract: Background: The novel coronavirus SARS-CoV-2 is the etiological agent of COVID-19. This virus has become one of the most dangerous in recent times with a very high rate of transmission. At present, several publications show the typical crown-shape of ... ...

Abstract	Background: The novel coronavirus SARS-CoV-2 is the etiological agent of COVID-19. This virus has become one of the most dangerous in recent times with a very high rate of transmission. At present, several publications show the typical crown-shape of the novel coronavirus grown in cell cultures. However, an integral ultramicroscopy study done directly from clinical specimens has not been published. Methods: Nasopharyngeal swabs were collected from 12 Cuban individuals, six asymptomatic and RT-PCR negative (negative control) and six others from a COVID-19 symptomatic and RT-PCR positive for SARS CoV-2. Samples were treated with an aldehyde solution and processed by scanning electron microscopy (SEM), confocal microscopy (CM) and, atomic force microscopy. Improvement and segmentation of coronavirus images were performed by a novel mathematical image enhancement algorithm. Results: The images of the negative control sample showed the characteristic healthy microvilli morphology at the apical region of the nasal epithelial cells. As expected, they do not display virus-like structures. The images of the positive sample showed characteristic coronavirus-like particles and evident destruction of microvilli. In some regions, virions budding through the cell membrane were observed. Microvilli destruction could explain the anosmia reported by some patients. Virus-particles emerging from the cell-surface with a variable size ranging from 80 to 400 nm were observed by SEM. Viral antigen was identified in the apical cells zone by CM. Conclusions: The integral microscopy study showed that SARS-CoV-2 has a similar image to SARS-CoV. The application of several high-resolution microscopy techniques to nasopharyngeal samples awaits future use.
MeSH term(s)	Antigens, Viral/metabolism ; COVID-19/diagnosis ; COVID-19/pathology ; COVID-19/virology ; Epithelial Cells/ultrastructure ; Epithelial Cells/virology ; Humans ; Image Enhancement ; Microscopy ; Microvilli/ultrastructure ; Nasal Mucosa/ultrastructure ; Nasal Mucosa/virology ; Nasopharynx/ultrastructure ; Nasopharynx/virology ; SARS-CoV-2/isolation & purification ; SARS-CoV-2/ultrastructure ; Virion/ultrastructure
Chemical Substances	Antigens, Viral
Language	English
Publishing date	2021-07-18
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1743-422X
ISSN (online)	1743-422X
DOI	10.1186/s12985-021-01620-1
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Article ; Online: The microglial NLRP3 inflammasome is involved in human SARS-CoV-2 cerebral pathogenicity: A report of three post-mortem cases.

Cama, Viviana Falcón / Marín-Prida, Javier / Acosta-Rivero, Nelson / Acosta, Emilio F / Díaz, Leonardo Oramas / Casadesús, Ana V / Fernández-Marrero, Briandy / Gilva-Rodríguez, Nathalie / Cremata-García, Daina / Cervantes-Llanos, Majel / Piniella-Matamoros, Beatriz / Sánchez, Daisy / Del Rosario-Cruz, Leticia / Borrajero, Israel / Díaz, Angelina / González, Yorexis / Pentón-Arias, Eduardo / Montero-González, Teresita / Guillen-Nieto, Gerardo /

Pentón-Rol, Giselle

Journal of neuroimmunology

2021 Volume 361, Page(s) 577728

Abstract: We herein report, by using confocal immunofluorescence, the colocalization of the SARS-CoV-2 nucleocapsid within neurons, astrocytes, oligodendrocytes and microglia in three deceased COVID-19 cases, of between 78 and 85 years of age at death. The viral ... ...

Abstract	We herein report, by using confocal immunofluorescence, the colocalization of the SARS-CoV-2 nucleocapsid within neurons, astrocytes, oligodendrocytes and microglia in three deceased COVID-19 cases, of between 78 and 85 years of age at death. The viral nucleocapsid was detected together with its ACE2 cell entry receptor, as well as the NLRP3 inflammasome in cerebral cortical tissues. It is noteworthy that NLRP3 was colocalized with CD68 + macrophages in the brain and lung of the deceased, suggesting the critical role of this type of inflammasome in SARS-CoV-2 lesions of the nervous system/lungs and supporting its potential role as a therapeutic target.
MeSH term(s)	Aged ; Aged, 80 and over ; Astrocytes/virology ; Autopsy ; Brain/immunology ; Brain/pathology ; Brain/virology ; COVID-19/immunology ; COVID-19/pathology ; COVID-19/virology ; Female ; Humans ; Inflammasomes/immunology ; Male ; Microglia/immunology ; Microglia/virology ; NLR Family, Pyrin Domain-Containing 3 Protein/immunology ; Neurons/virology ; Nucleocapsid ; Oligodendroglia/virology ; SARS-CoV-2/pathogenicity
Chemical Substances	Inflammasomes ; NLR Family, Pyrin Domain-Containing 3 Protein ; NLRP3 protein, human
Language	English
Publishing date	2021-09-29
Publishing country	Netherlands
Document type	Case Reports ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	8335-5
ISSN	1872-8421 ; 0165-5728
ISSN (online)	1872-8421
ISSN	0165-5728
DOI	10.1016/j.jneuroim.2021.577728
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Article ; Online: Evidence of SARS-CoV-2 infection in postmortem lung, kidney, and liver samples, revealing cellular targets involved in COVID-19 pathogenesis.

Falcón-Cama, Viviana / Montero-González, Teresita / Acosta-Medina, Emilio F / Guillen-Nieto, Gerardo / Berlanga-Acosta, Jorge / Fernández-Ortega, Celia / Alfonso-Falcón, Anabel / Gilva-Rodríguez, Nathalie / López-Nocedo, Lilianne / Cremata-García, Daina / Matos-Terrero, Mariuska / Pentón-Rol, Giselle / Valdés, Iris / Oramas-Díaz, Leonardo / Suarez-Batista, Anamarys / Noa-Romero, Enrique / Cruz-Sui, Otto / Sánchez, Daisy / Borrego-Díaz, Amanda I /

Valdés-Carreras, Juan E / Vizcaino, Ananayla / Suárez-Alba, José / Valdés-Véliz, Rodolfo / Bergado, Gretchen / González, Miguel A / Hernandez, Tays / Alvarez-Arzola, Rydell / Ramírez-Suárez, Anna C / Casillas-Casanova, Dionne / Lemos-Pérez, Gilda / Blanco-Águila, Omar R / Díaz, Angelina / González, Yorexis / Bequet-Romero, Mónica / Marín-Prida, Javier / Hernández-Perera, Julio C / Del Rosario-Cruz, Leticia / Marin-Díaz, Alina P / González-Bravo, Maritza / Borrajero, Israel / Acosta-Rivero, Nelson

Archives of virology

2023 Volume 168, Issue 3, Page(s) 96

Abstract: There is an urgent need to understand severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-host interactions involved in virus spread and pathogenesis, which might contribute to the identification of new therapeutic targets. In this study, we ... ...

Abstract	There is an urgent need to understand severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-host interactions involved in virus spread and pathogenesis, which might contribute to the identification of new therapeutic targets. In this study, we investigated the presence of SARS-CoV-2 in postmortem lung, kidney, and liver samples of patients who died with coronavirus disease (COVID-19) and its relationship with host factors involved in virus spread and pathogenesis, using microscopy-based methods. The cases analyzed showed advanced stages of diffuse acute alveolar damage and fibrosis. We identified the SARS-CoV-2 nucleocapsid (NC) in a variety of cells, colocalizing with mitochondrial proteins, lipid droplets (LDs), and key host proteins that have been implicated in inflammation, tissue repair, and the SARS-CoV-2 life cycle (vimentin, NLRP3, fibronectin, LC3B, DDX3X, and PPARγ), pointing to vimentin and LDs as platforms involved not only in the viral life cycle but also in inflammation and pathogenesis. SARS-CoV-2 isolated from a patient´s nasal swab was grown in cell culture and used to infect hamsters. Target cells identified in human tissue samples included lung epithelial and endothelial cells; lipogenic fibroblast-like cells (FLCs) showing features of lipofibroblasts such as activated PPARγ signaling and LDs; lung FLCs expressing fibronectin and vimentin and macrophages, both with evidence of NLRP3- and IL1β-induced responses; regulatory cells expressing immune-checkpoint proteins involved in lung repair responses and contributing to inflammatory responses in the lung; CD34
MeSH term(s)	Humans ; COVID-19/pathology ; Fibronectins ; Vimentin ; SARS-CoV-2 ; Endothelial Cells ; NLR Family, Pyrin Domain-Containing 3 Protein ; PPAR gamma ; Lung ; Inflammation/pathology ; Kidney ; Liver
Chemical Substances	Fibronectins ; Vimentin ; NLR Family, Pyrin Domain-Containing 3 Protein ; PPAR gamma
Language	English
Publishing date	2023-02-26
Publishing country	Austria
Document type	Journal Article
ZDB-ID	7491-3
ISSN	1432-8798 ; 0304-8608
ISSN (online)	1432-8798
ISSN	0304-8608
DOI	10.1007/s00705-023-05711-y
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Article ; Online: Evidence of SARS-CoV-2 infection in postmortem lung, kidney, and liver samples, revealing cellular targets involved in COVID-19 pathogenesis

Falcón-Cama, Viviana / Montero-González, Teresita / Acosta-Medina, Emilio F. / Guillen-Nieto, Gerardo / Berlanga-Acosta, Jorge / Fernández-Ortega, Celia / Alfonso-Falcón, Anabel / Gilva-Rodríguez, Nathalie / López-Nocedo, Lilianne / Cremata-García, Daina / Matos-Terrero, Mariuska / Penton-Rol, Giselle / Valdés, Iris / Oramas-Díaz, Leonardo / Suarez-Batista, Anamarys / Noa-Romero, Enrique / Cruz-Sui, Otto / Sánchez, Daisy / Borrego-Díaz, Amanda I. /

Valdés-Carreras, Juan E. / Vizcaino, Ananayla / Suárez-Alba, José / Valdés-Véliz, Rodolfo / Bergado, Gretchen / González, Miguel A. / Hernandez, Tays / Alvarez-Arzola, Rydell / Ramírez-Suárez, Anna C. / Casillas-Casanova, Dionne / Lemos-Pérez, Gilda / Blanco-Águila, Omar R. / Díaz, Angelina / González, Yorexis / Bequet-Romero, Mónica / Marín-Prida, Javier / Hernández-Perera, Julio C. / del Rosario-Cruz, Leticia / Marin-Díaz, Alina P. / González-Bravo, Maritza / Borrajero, Israel / Acosta-Rivero, Nelson

Arch Virol. 2023 Mar., v. 168, no. 3 p.96-96

2023

Abstract	There is an urgent need to understand severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-host interactions involved in virus spread and pathogenesis, which might contribute to the identification of new therapeutic targets. In this study, we investigated the presence of SARS-CoV-2 in postmortem lung, kidney, and liver samples of patients who died with coronavirus disease (COVID-19) and its relationship with host factors involved in virus spread and pathogenesis, using microscopy-based methods. The cases analyzed showed advanced stages of diffuse acute alveolar damage and fibrosis. We identified the SARS-CoV-2 nucleocapsid (NC) in a variety of cells, colocalizing with mitochondrial proteins, lipid droplets (LDs), and key host proteins that have been implicated in inflammation, tissue repair, and the SARS-CoV-2 life cycle (vimentin, NLRP3, fibronectin, LC3B, DDX3X, and PPARγ), pointing to vimentin and LDs as platforms involved not only in the viral life cycle but also in inflammation and pathogenesis. SARS-CoV-2 isolated from a patient´s nasal swab was grown in cell culture and used to infect hamsters. Target cells identified in human tissue samples included lung epithelial and endothelial cells; lipogenic fibroblast-like cells (FLCs) showing features of lipofibroblasts such as activated PPARγ signaling and LDs; lung FLCs expressing fibronectin and vimentin and macrophages, both with evidence of NLRP3- and IL1β-induced responses; regulatory cells expressing immune-checkpoint proteins involved in lung repair responses and contributing to inflammatory responses in the lung; CD34⁺ liver endothelial cells and hepatocytes expressing vimentin; renal interstitial cells; and the juxtaglomerular apparatus. This suggests that SARS-CoV-2 may directly interfere with critical lung, renal, and liver functions involved in COVID-19-pathogenesis.
Keywords	COVID-19 infection ; Severe acute respiratory syndrome coronavirus 2 ; cell culture ; epithelium ; fibronectins ; fibrosis ; hepatocytes ; humans ; inflammation ; kidneys ; lipids ; liver ; lungs ; macrophages ; mitochondria ; nose ; nucleocapsid ; pathogenesis ; patients ; therapeutics ; tissue repair ; vimentin ; viruses
Language	English
Dates of publication	2023-03
Size	p. 96.
Publishing place	Springer Vienna
Document type	Article ; Online
ZDB-ID	7491-3
ISSN	1432-8798 ; 0304-8608
ISSN (online)	1432-8798
ISSN	0304-8608
DOI	10.1007/s00705-023-05711-y
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Article: Treatment of rectovaginal fistula: a 5-year review.

Casadesus, Damian / Villasana, Luis / Sanchez, Ines M / Diaz, Hector / Chavez, Mariano / Diaz, Angelina

The Australian & New Zealand journal of obstetrics & gynaecology

2006 Volume 46, Issue 1, Page(s) 49–51

Abstract: This paper presents a chart review of 17 patients who had been treated for rectovaginal fistula (RVF) from 1996 to 2000. In most cases (13; 76.5%), the fistula was the result of post-surgical complications. Following vaginal mucosa advancement flap ... ...

Abstract	This paper presents a chart review of 17 patients who had been treated for rectovaginal fistula (RVF) from 1996 to 2000. In most cases (13; 76.5%), the fistula was the result of post-surgical complications. Following vaginal mucosa advancement flap repair or repair after conversion to a fourth-degree perineal laceration, 16 (94%) of the rectovaginal fistulae (during the first attempted repair or after failed treatment) were successfully treated. In all patients but one, faecal diversion was avoided. In two patients, fistulography was both a diagnostic procedure and the method of treatment.
MeSH term(s)	Adult ; Aged ; Female ; Humans ; Medical Records ; Middle Aged ; Perineum/surgery ; Postoperative Complications ; Rectovaginal Fistula/diagnosis ; Rectovaginal Fistula/etiology ; Rectovaginal Fistula/surgery ; Rectum/surgery ; Reoperation ; Retrospective Studies ; Surgical Flaps ; Treatment Outcome ; Wound Healing
Language	English
Publishing date	2006-02
Publishing country	Australia
Document type	Journal Article
ZDB-ID	390815-x
ISSN	1479-828X ; 0004-8666
ISSN (online)	1479-828X
ISSN	0004-8666
DOI	10.1111/j.1479-828X.2006.00514.x
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