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  1. Article ; Online: Enhancement of Mitochondrial Function by the Neurogenic Molecule NSI-189 Accompanies Reversal of Peripheral Neuropathy and Memory Impairment in a Rat Model of Type 2 Diabetes.

    Jolivalt, C G / Aghanoori, M R / Navarro-Diaz, M C / Han, M M / Sanchez, G / Guernsey, L / Quach, D / Johe, K / Fernyhough, P / Calcutt, N A

    Journal of diabetes research

    2022  Volume 2022, Page(s) 8566970

    Abstract: Aims: Mitochondrial dysfunction contributes to many forms of peripheral and central nervous system degeneration. Therapies that protect mitochondrial number and function have the potential to impact the progression of conditions such as diabetic ... ...

    Abstract Aims: Mitochondrial dysfunction contributes to many forms of peripheral and central nervous system degeneration. Therapies that protect mitochondrial number and function have the potential to impact the progression of conditions such as diabetic neuropathy. We therefore assessed indices of mitochondrial function in dorsal root ganglia (DRG) and brain cortex of the Zucker diabetic fatty (ZDF) rat model of type 2 diabetes and tested the therapeutic impact of a neurogenic compound, NSI-189, on both mitochondrial function and indices of peripheral and central neurological dysfunction.
    Materials and methods: ZDF rats were maintained for 16 weeks of untreated diabetes before the start of oral treatment with NSI-189 for an additional 16 weeks. Nerve conduction velocity, sensitivity to tactile and thermal stimuli, and behavioral assays of cognitive function were assessed monthly. AMP-activated protein kinase (AMPK) phosphorylation, mitochondrial protein levels, and respiratory complex activities were assessed in the DRG and brain cortex after 16 weeks of treatment with NSI-189.
    Results: Treatment with NSI-189 selectively elevated the expression of protein subunits of complexes III and V and activities of respiratory complexes I and IV in the brain cortex, and this was accompanied by amelioration of impaired memory function and plasticity. In the sensory ganglia of ZDF rats, loss of AMPK activity was ameliorated by NSI-189, and this was accompanied by reversal of multiple indices of peripheral neuropathy.
    Conclusions: Efficacy of NSI-189 against dysfunction of the CNS and PNS function in type 2 diabetic rats was accompanied by improvement of mitochondrial function. NSI-189 exhibited actions at different levels of mitochondrial regulation in central and peripheral tissues.
    MeSH term(s) AMP-Activated Protein Kinases/metabolism ; Aminopyridines ; Animals ; Diabetes Mellitus, Experimental/metabolism ; Diabetes Mellitus, Type 2/metabolism ; Diabetic Neuropathies ; Mitochondria/metabolism ; Piperazines ; Rats ; Rats, Zucker
    Chemical Substances Aminopyridines ; NSI-189 ; Piperazines ; AMP-Activated Protein Kinases (EC 2.7.11.31)
    Language English
    Publishing date 2022-07-04
    Publishing country England
    Document type Journal Article
    ZDB-ID 2711897-6
    ISSN 2314-6753 ; 2314-6753
    ISSN (online) 2314-6753
    ISSN 2314-6753
    DOI 10.1155/2022/8566970
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Lectin histochemistry for detecting cadmium-induced changes in the glycosylation pattern of rat placenta.

    Díaz, M C / González, N V / Zanuzzi, C N / Najle, R / Barbeito, C G

    Biotechnic & histochemistry : official publication of the Biological Stain Commission

    2017  Volume 92, Issue 1, Page(s) 36–45

    Abstract: Cadmium (Cd) is an industrial and environmental pollutant that produces toxic effects on gametogenesis, pre- and post-implantation embryos, and the placenta. Because the effects of acute Cd intoxication on the placenta are not well understood, we ... ...

    Abstract Cadmium (Cd) is an industrial and environmental pollutant that produces toxic effects on gametogenesis, pre- and post-implantation embryos, and the placenta. Because the effects of acute Cd intoxication on the placenta are not well understood, we investigated changes in its glycosylated components in Cd treated dams at days 4, 7, 10 and 15 of gestation using lectin histochemistry. CdCl
    MeSH term(s) Animals ; Cadmium/toxicity ; Female ; Glycosylation ; Lectins/chemistry ; Placenta/drug effects ; Placenta/metabolism ; Pregnancy ; Rats ; Rats, Wistar ; Trophoblasts/drug effects ; Trophoblasts/physiology
    Chemical Substances Lectins ; Cadmium (00BH33GNGH)
    Language English
    Publishing date 2017
    Publishing country England
    Document type Journal Article
    ZDB-ID 1069349-x
    ISSN 1473-7760 ; 1052-0295
    ISSN (online) 1473-7760
    ISSN 1052-0295
    DOI 10.1080/10520295.2016.1185668
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Patterns of proliferation and cell differentiation during hepatic ontogeny in the alpaca.

    Castro, A N C / Díaz, M C / Mendoza Torres, G J / Moreno Burgos, B / Zanuzzi, C / Illia, M Carrica / Lendez, P A / Carril, J / Ghezzi, M D / Bodiola Diez, J J / Barbeito, C G

    Tissue & cell

    2021  Volume 71, Page(s) 101589

    Abstract: The liver has multiple functions that change throughout ontogeny. South American camelids (SAC) have unique characteristics related to adaptation to extreme environments and metabolism. However, the process of hepatic cell differentiation has not been ... ...

    Abstract The liver has multiple functions that change throughout ontogeny. South American camelids (SAC) have unique characteristics related to adaptation to extreme environments and metabolism. However, the process of hepatic cell differentiation has not been studied in any SAC. We study the patterns of cell differentiation and proliferation in the liver of the alpaca at different times of the ontogeny, excluding the hematopoietic components. Immunohistochemical techniques were performed in 66 specimens, including embryos, fetuses, neonates and adults. Supplementary analyses were performed by lectinhistochemistry. The hepatocytic differentiation was performed by the identification of Hepatocyte (Clone: ​​OCH1ES Dako®). It began in the specimens of 1.8-2.5 cm of crown to rump length (CRL), from Days 25-29 (ovulation = Day 0), continued during gestation and intensified towards its end. The cholangiocytic differentiation was performed by the identification of cytokeratin 7 (CK7, Dako®). It was manifested at the final of gestation (specimens of 28.4 cm CRL, from Day 223 onwards). Parenchymal cells underwent a process of gradual differentiation (differentiation of hepatocytes preceded that of cholangiocytes). Cell proliferation was observed along gestation using the nuclear proliferation antigen (PCNA) and Ki-67. Hepatic organogenesis in the alpacas shares similar differentiation and proliferation mechanisms with other altricial, but phylogenetically distant, species.
    MeSH term(s) Animals ; Antigens, Differentiation/metabolism ; Camelids, New World/embryology ; Cell Differentiation ; Cell Proliferation ; Female ; Hepatocytes/metabolism ; Liver/embryology ; Male
    Chemical Substances Antigens, Differentiation
    Language English
    Publishing date 2021-07-01
    Publishing country Scotland
    Document type Journal Article
    ZDB-ID 204424-9
    ISSN 1532-3072 ; 0040-8166
    ISSN (online) 1532-3072
    ISSN 0040-8166
    DOI 10.1016/j.tice.2021.101589
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Morphology of alpaca (Vicugna pacos) embryos in the first third of pregnancy.

    Castro, Anc / Díaz, M C / Mendoza-Torres, G J / Llerena-Zavala, C A / Ghezzi, M D / Barbeito, C G

    Reproduction in domestic animals = Zuchthygiene

    2018  Volume 53, Issue 3, Page(s) 655–666

    Abstract: The breeding of South American camelids is the main economic activity of the high Andean region of South America and it, is potentially, the most profitable resource in of the Puna environmental conditions of the Puna. The duration of the gestation in ... ...

    Abstract The breeding of South American camelids is the main economic activity of the high Andean region of South America and it, is potentially, the most profitable resource in of the Puna environmental conditions of the Puna. The duration of the gestation in alpaca is 339.7 ± 12 days. The objective of the present work was to macroscopically and microscopically describe the ontogenic development of the splanchnic cavities of the alpaca and to determine the gestational time in which the post-cranial ossification centers are observed in the embryos/fetuses of this species, from day 21 to 107 of gestation. The documentation of normal ontogenic development, which is vacant for this period, is of the utmost importance to understand the consequences of the alterations at the different gestational times, as well as for the estimation of the gestational age in the case of abortions. Forty-seven alpaca specimens of both sexes, at different times of their gestational development, collected during slaughter at local slaughterhouses of the Department of Huancavelica, Peru, were evaluated. Specimens were assigned to seven groups according to their morphological characteristics. The embryogenesis in the alpaca was characterized by a series of changes comparable to those occurring in other mammals with similar gestational periods. Despite these similarities, species differences were found in some organs as stomach, which are observed too in adult individuals.
    MeSH term(s) Animals ; Camelids, New World/embryology ; Embryo, Mammalian/anatomy & histology ; Embryonic Development ; Female ; Gestational Age ; Male ; Osteogenesis ; Pregnancy ; Stomach/embryology
    Language English
    Publishing date 2018-03-22
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1015187-4
    ISSN 1439-0531 ; 0936-6768
    ISSN (online) 1439-0531
    ISSN 0936-6768
    DOI 10.1111/rda.13155
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Physician-nurse camaraderie.

    Diaz, M C

    AORN journal

    1994  Volume 59, Issue 5, Page(s) 964

    MeSH term(s) California ; Humans ; Interprofessional Relations ; Nurses ; Physicians
    Language English
    Publishing date 1994-05
    Publishing country United States
    Document type Comment ; Letter
    ZDB-ID 603972-8
    ISSN 0001-2092
    ISSN 0001-2092
    DOI 10.1016/s0001-2092(07)65503-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Laboratory exposure to Coccidioides: lessons learnt in a non-endemic country.

    Porte, L / Valdivieso, F / Wilmes, D / Gaete, P / Díaz, M C / Thompson, L / Munita, J M / Alliende, R / Varela, C / Rickerts, V / Weitzel, T

    The Journal of hospital infection

    2019  Volume 102, Issue 4, Page(s) 461–464

    Abstract: Coccidioides is a primary pathogenic fungus, which infects humans through highly infectious arthroconidia, causing substantial morbidity including life-threatening disseminated infections. Due to the low infectious dose, laboratory personnel might become ...

    Abstract Coccidioides is a primary pathogenic fungus, which infects humans through highly infectious arthroconidia, causing substantial morbidity including life-threatening disseminated infections. Due to the low infectious dose, laboratory personnel might become infected during diagnostic procedures. Accordingly, coccidioidomycosis is reported as the most frequent laboratory-acquired systemic mycosis worldwide. This risk is aggravated in non-endemic countries, where the diagnosis may not be suspected. We report on an inadvertent exposure of 44 persons to Coccidioides posadasii in a clinical microbiology laboratory in Chile, the measures of containment after rapid diagnosis with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and the lessons learnt in a non-endemic setting.
    MeSH term(s) Chile/epidemiology ; Coccidioides/isolation & purification ; Coccidioidomycosis/diagnosis ; Coccidioidomycosis/epidemiology ; Coccidioidomycosis/microbiology ; Humans ; Infection Control ; Laboratory Infection/diagnosis ; Laboratory Infection/epidemiology ; Laboratory Infection/microbiology ; Microbiological Techniques ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
    Language English
    Publishing date 2019-03-16
    Publishing country England
    Document type Journal Article
    ZDB-ID 779366-2
    ISSN 1532-2939 ; 0195-6701
    ISSN (online) 1532-2939
    ISSN 0195-6701
    DOI 10.1016/j.jhin.2019.03.006
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  7. Article ; Online: Nocardiosis abdominal seudotumoral: una presentación clínica infrecuente.

    Rojo-Valencia, V / Ferreiro-López, D / Montes-Rodríguez, I / Méndez-Díaz, M C

    Revista clinica espanola

    2014  Volume 214, Issue 4, Page(s) 230–231

    Title translation Pseudotumoral abdominal nocardiosis: an uncommon clinical presentation.
    MeSH term(s) Abdominal Pain/etiology ; Aged, 80 and over ; Humans ; Intraabdominal Infections/diagnosis ; Intraabdominal Infections/microbiology ; Intraabdominal Infections/physiopathology ; Male ; Nocardia Infections/diagnosis ; Nocardia Infections/physiopathology ; Peritoneal Neoplasms/diagnosis
    Language Spanish
    Publishing date 2014-05
    Publishing country Spain
    Document type Case Reports ; Letter
    ISSN 2254-8874
    ISSN (online) 2254-8874
    DOI 10.1016/j.rce.2014.01.020
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  8. Article ; Online: Identificación y sensibilidad antifúngica in vitro de Candida spp. de origen vaginal a fluconazol, clotrimazol y nistatina.

    Diaz, M C / Camponovo, R / Araya, I / Cerda, A / Santander, M P / Carrillo-Muñoz, A J

    Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia

    2016  Volume 29, Issue 3, Page(s) 151–154

    Abstract: Objective: The aim of this study was to identify and determine the in vitro antifungal susceptibility testing to clotrimazole, fluconazole, and nystatin of 145 clinical isolates of Candida spp.: Methods: M27-A3 microdilution method was used to ... ...

    Title translation Identification and in vitro antifungal susceptibility of vaginal Candida spp. isolates to fluconazole, clotrimazole and nystatin.
    Abstract Objective: The aim of this study was to identify and determine the in vitro antifungal susceptibility testing to clotrimazole, fluconazole, and nystatin of 145 clinical isolates of Candida spp.
    Methods: M27-A3 microdilution method was used to determine minimal inhibitory concentrations (MIC) and partial MICs (MIC50 and MIC90) of drugs. A total of 145 isolates were studied, 126 were C. albicans, 16 C. glabrata, 2 C. parapsilosis y 1 C. tropicalis.
    Results: MIC50 and MIC90 for FLZ against C. albicans were 0.25 mg/L and 1 mg/L respectively and for C. glabrata was achieved at 8 mg/L and 16 mg/L for fluconazole. Five isolates of C. albicans and one isolate of C. tropicalis were in vitro resistant to fluconazole (M27-S4). In C. albicans MIC50 and MIC90 for clotrimazole were of 0.03 mg/L and 0.06 mg/L, respectively. These values for C. glabrata were 0.25 mg/L and 1 mg/L, respectively. Five C. glabrata and 1 C. tropicalis were in vitro resistant to clotrimazole. MIC50 and MIC90 of nystatin were of 1 mg/L and 2 mg/L, respectively for C. albicans and C. glabrata.
    Conclusions: In this study, C. albicans is the most frequently isolated yeast, followed by C. glabrata. The antifungals tested were found to be in vitro active for the isolates, except for 6 isolates for fluconazole and 6 to clotrimazole.
    MeSH term(s) Antifungal Agents/pharmacology ; Candida/drug effects ; Candidiasis, Vulvovaginal/epidemiology ; Candidiasis, Vulvovaginal/microbiology ; Clotrimazole/pharmacology ; Drug Resistance, Fungal ; Female ; Fluconazole/pharmacology ; Humans ; Microbial Sensitivity Tests ; Nystatin/pharmacology
    Chemical Substances Antifungal Agents ; Nystatin (1400-61-9) ; Fluconazole (8VZV102JFY) ; Clotrimazole (G07GZ97H65)
    Language Spanish
    Publishing date 2016-06
    Publishing country Spain
    Document type Journal Article
    ZDB-ID 1018135-0
    ISSN 1988-9518 ; 0214-3429
    ISSN (online) 1988-9518
    ISSN 0214-3429
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  9. Article: Toxicity assessment of cobalt ferrite nanoparticles on wheat plants.

    López-Luna, J / Camacho-Martínez, M M / Solís-Domínguez, F A / González-Chávez, M C / Carrillo-González, R / Martinez-Vargas, S / Mijangos-Ricardez, O F / Cuevas-Díaz, M C

    Journal of toxicology and environmental health. Part A

    2018  Volume 81, Issue 14, Page(s) 604–619

    Abstract: Cobalt ferrite nanoparticles (NPs) have received increasing attention due to their widespread therapeutic and agricultural applicability. In the environmental field, dry powder- and ferrofluid-suspended cobalt ferrite NPs were found to be useful for ... ...

    Abstract Cobalt ferrite nanoparticles (NPs) have received increasing attention due to their widespread therapeutic and agricultural applicability. In the environmental field, dry powder- and ferrofluid-suspended cobalt ferrite NPs were found to be useful for removing heavy metals and metalloids from water, while diluted suspensions of cobalt ferrite NP have been promisingly applied in medicine. However, the potential toxicological implications of widespread exposure are still unknown. Since cobalt ferrite NPs are considered residual wastes of environmental or medical applications, plants may serve as a point-of-entry for engineered nanomaterials as a result of consumption of these plants. Thus, the aim of this study was to assess the effects of dry powder and fresh cobalt ferrite NP on wheat plants. Seven-day assays were conducted, using quartz sand as the plant growth substrate. The toxicity end points measured were seed germination, root and shoot lengths, total cobalt (Co) and iron (Fe) accumulation, photosynthetic pigment production, protein (PRT) production, and activities of catalase (CAT), ascorbate peroxidase (APX), and guaiacol peroxidase (GPX). Increasing total Co and Fe in plant tissues indicated that wheat plants were exposed to cobalt ferrite NP. Seed germination and shoot length were not sufficiently sensitive toxicity end points. The effective concentration (EC
    MeSH term(s) Antioxidants/metabolism ; Cobalt/chemistry ; Cobalt/toxicity ; Ferric Compounds/chemistry ; Ferric Compounds/toxicity ; Germination/drug effects ; Metal Nanoparticles ; Oxidative Stress ; Photosynthesis/drug effects ; Pigments, Biological/metabolism ; Plant Proteins/biosynthesis ; Powders/chemistry ; Powders/toxicity ; Seedlings/growth & development ; Seeds/drug effects ; Seeds/enzymology ; Seeds/growth & development ; Seeds/physiology ; Toxicity Tests ; Triticum/drug effects ; Triticum/enzymology ; Triticum/growth & development ; Triticum/physiology
    Chemical Substances Antioxidants ; Ferric Compounds ; Pigments, Biological ; Plant Proteins ; Powders ; cobalt ferrite ; Cobalt (3G0H8C9362)
    Language English
    Publishing date 2018-05-08
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1413345-3
    ISSN 1528-7394 ; 0098-4108 ; 1087-2620
    ISSN 1528-7394 ; 0098-4108 ; 1087-2620
    DOI 10.1080/15287394.2018.1469060
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  10. Article ; Online: Low rate of prenatal diagnosis among neonates with critical aortic stenosis: insight into the natural history in utero.

    Freud, L R / Moon-Grady, A / Escobar-Diaz, M C / Gotteiner, N L / Young, L T / McElhinney, D B / Tworetzky, W

    Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology

    2015  Volume 45, Issue 3, Page(s) 326–332

    Abstract: Objectives: To better understand the natural history and spectrum of fetal aortic stenosis (AS), we aimed to (1) determine the prenatal diagnosis rate of neonates with critical AS and a biventricular (BV) outcome, and (2) describe the findings at fetal ... ...

    Abstract Objectives: To better understand the natural history and spectrum of fetal aortic stenosis (AS), we aimed to (1) determine the prenatal diagnosis rate of neonates with critical AS and a biventricular (BV) outcome, and (2) describe the findings at fetal echocardiography in patients diagnosed prenatally.
    Methods: A multicenter, retrospective study was performed on neonates who presented with critical AS and who were discharged with a BV outcome from 2000 to 2013. The prenatal diagnosis rate was compared with that reported for hypoplastic left heart syndrome (HLHS). We reviewed fetal echocardiographic findings in patients who were diagnosed prenatally.
    Results: In only 10 (8.5%) of 117 neonates with critical AS and a BV outcome was the diagnosis made prenatally, a rate significantly lower than that for HLHS in the contemporary era (82%; P < 0.0001). Of the 10 patients diagnosed prenatally, all had developed left ventricular dysfunction by a median gestational age of 33 (range, 28-35) weeks. When present, Doppler abnormalities such as retrograde flow in the aortic arch (n = 2), monophasic mitral inflow (n = 3) and left-to-right flow across the foramen ovale (n = 8) developed late in gestation (median 33 weeks).
    Conclusion: The prenatal diagnosis rate of critical AS and a BV outcome among neonates is very low, probably owing to a relatively normal four-chamber view in mid-gestation with development of significant obstruction in the third trimester. The natural history contrasts with that of severe mid-gestation AS with evolving HLHS and suggests that the gestational timing of development of significant AS has an important impact on subsequent left-heart growth in utero.
    MeSH term(s) Aortic Valve Stenosis/diagnosis ; Aortic Valve Stenosis/embryology ; Electrocardiography ; Female ; Gestational Age ; Heart Rate, Fetal ; Humans ; Infant, Newborn ; Male ; Pregnancy ; Pregnancy Trimester, Third ; Retrospective Studies ; Ultrasonography, Prenatal ; United States
    Language English
    Publishing date 2015-01-28
    Publishing country England
    Document type Journal Article ; Multicenter Study ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 1073183-0
    ISSN 1469-0705 ; 0960-7692
    ISSN (online) 1469-0705
    ISSN 0960-7692
    DOI 10.1002/uog.14667
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