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  1. Book ; Online: Algal Biomass, Biofuels and Bioproducts

    D'Adamo, Sarah

    2023  

    Abstract: Invited speaker: The 10th International Conference on Algal Biomass, Biofuels and Bioproducts (ABBB)’’ 12–14 June 2023 | Waikoloa Beach, Hawaii, ... ...

    Abstract Invited speaker: The 10th International Conference on Algal Biomass, Biofuels and Bioproducts (ABBB)’’ 12–14 June 2023 | Waikoloa Beach, Hawaii, USA
    Keywords Life Science
    Publishing country nl
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Expression of glycerol-3-phosphate acyltransferase increases non-polar lipid accumulation in Nannochloropsis oceanica.

    Südfeld, Christian / Kiyani, Aamna / Wefelmeier, Katrin / Wijffels, René H / Barbosa, Maria J / D'Adamo, Sarah

    Microbial cell factories

    2023  Volume 22, Issue 1, Page(s) 12

    Abstract: Microalgae are considered a suitable production platform for high-value lipids and oleochemicals. Several species including Nannochloropsis oceanica produce large amounts of essential [Formula: see text]-3 polyunsaturated fatty acids (PUFAs) which are ... ...

    Abstract Microalgae are considered a suitable production platform for high-value lipids and oleochemicals. Several species including Nannochloropsis oceanica produce large amounts of essential [Formula: see text]-3 polyunsaturated fatty acids (PUFAs) which are integral components of food and feed and have been associated with health-promoting effects. N. oceanica can further accumulate high contents of non-polar lipids with chemical properties that render them a potential replacement for plant oils such as palm oil. However, biomass and lipid productivities obtained with microalgae need to be improved to reach commercial feasibility. Genetic engineering can improve biomass and lipid productivities, for instance by increasing carbon flux to lipids. Here, we report the overexpression of glycerol-3-phosphate acyltransferase (GPAT) in N. oceanica during favorable growth conditions as a strategy to increase non-polar lipid content. Transformants overproducing either an endogenous (NoGPAT) or a heterologous (Acutodesmus obliquus GPAT) GPAT enzyme targeted to the endoplasmic reticulum had up to 42% and 51% increased non-polar lipid contents, respectively, compared to the wild type. Biomass productivities of transformant strains were not substantially impaired, resulting in lipid productivities that were increased by up to 37% and 42% for NoGPAT and AoGPAT transformants, respectively. When exposed to nutrient stress, transformants and wild type had similar lipid contents, suggesting that GPAT enzyme exerts strong flux control on lipid synthesis in N. oceanica under favorable growth conditions. NoGPAT transformants further accumulated PUFAs in non-polar lipids, reaching a total of 6.8% PUFAs per biomass, an increase of 24% relative to the wild type. Overall, our results indicate that GPAT is an interesting target for engineering of lipid metabolism in microalgae, in order to improve non-polar lipid and PUFAs accumulation in microalgae.
    MeSH term(s) Glycerol/metabolism ; Oils/metabolism ; Genetic Engineering ; Glycerol-3-Phosphate O-Acyltransferase/genetics ; Glycerol-3-Phosphate O-Acyltransferase/metabolism ; Stramenopiles/genetics ; Microalgae/genetics ; Microalgae/metabolism ; Biomass ; Phosphates/metabolism
    Chemical Substances Glycerol (PDC6A3C0OX) ; Oils ; Glycerol-3-Phosphate O-Acyltransferase (EC 2.3.1.15) ; Phosphates
    Language English
    Publishing date 2023-01-16
    Publishing country England
    Document type Journal Article
    ZDB-ID 2091377-1
    ISSN 1475-2859 ; 1475-2859
    ISSN (online) 1475-2859
    ISSN 1475-2859
    DOI 10.1186/s12934-022-01987-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Hypes, hopes, and the way forward for microalgal biotechnology.

    Barbosa, Maria J / Janssen, Marcel / Südfeld, Christian / D'Adamo, Sarah / Wijffels, Rene H

    Trends in biotechnology

    2023  Volume 41, Issue 3, Page(s) 452–471

    Abstract: The urge for food security and sustainability has advanced the field of microalgal biotechnology. Microalgae are microorganisms able to grow using (sun)light, fertilizers, sugars, ... ...

    Abstract The urge for food security and sustainability has advanced the field of microalgal biotechnology. Microalgae are microorganisms able to grow using (sun)light, fertilizers, sugars, CO
    MeSH term(s) Microalgae/genetics ; Microalgae/metabolism ; Fertilizers ; Biotechnology ; Crops, Agricultural ; Biomass
    Chemical Substances Fertilizers
    Language English
    Publishing date 2023-01-25
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 47474-5
    ISSN 1879-3096 ; 0167-7799
    ISSN (online) 1879-3096
    ISSN 0167-7799
    DOI 10.1016/j.tibtech.2022.12.017
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2750: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  5. Book ; Online: 'Following Phaeodactylum tricornutum cell fate during nitrogen limitation and light/dark cycles by orchestrated “omics” analysis

    D'Adamo, Sarah

    2019  

    Keywords Life Science
    Language English
    Publishing country nl
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article: Prospects for viruses infecting eukaryotic microalgae in biotechnology

    D'Adamo, Sarah / Kormelink, Richard / Martens, Dirk / Barbosa, Maria J. / Wijffels, Rene H.

    Biotechnology advances. 2022 Jan., Feb., v. 54

    2022  

    Abstract: Besides being considered pathogens, viruses are important drivers of evolution and they can shape large ecological and biogeochemical processes, by influencing host fitness, population dynamics, and community structures. Moreover, they are simple systems ...

    Abstract Besides being considered pathogens, viruses are important drivers of evolution and they can shape large ecological and biogeochemical processes, by influencing host fitness, population dynamics, and community structures. Moreover, they are simple systems that can be used and manipulated to be beneficial and useful for biotechnological applications. In this context, microalgae biotechnology is a growing field of research, which investigated the usage of photosynthetic microorganisms for the sustainable production of food, fuel, chemical, and pharmaceutical sectors. Viruses infecting microalgae have become important subject of ecological studies related to marine and aquatic environments only four decades ago when virus-like-particles associated with bloom-forming algae were discovered. These first findings have opened new questions on evolution and identity. To date, 63 viruses that infect eukaryotic microalgae have been isolated and cultured. In this short review we briefly summarize what is known about viruses infecting eukaryotic microalgae, and how acknowledging their importance can shape future research focussed not only on marine ecology and evolutionary biology but also on biotechnological applications related to microalgae cell factories.
    Keywords biotechnology ; evolution ; marine science ; microalgae ; photosynthesis ; population dynamics
    Language English
    Dates of publication 2022-01
    Publishing place Elsevier Inc.
    Document type Article
    ZDB-ID 47165-3
    ISSN 0734-9750
    ISSN 0734-9750
    DOI 10.1016/j.biotechadv.2021.107790
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3730: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  7. Book ; Online: Expanding the tools for microalgal synbio research

    D'Adamo, Sarah

    2018  

    Keywords Life Science
    Language English
    Publishing country nl
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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    Inter-library loan at ZB MED

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  8. Article ; Online: Hypes, hopes, and the way forward for microalgal biotechnology

    Barbosa, Maria J. / Janssen, Marcel / Südfeld, Christian / D'Adamo, Sarah / Wijffels, Rene H.

    Trends in Biotechnology

    2023  Volume 41, Issue 3

    Abstract: The urge for food security and sustainability has advanced the field of microalgal biotechnology. Microalgae are microorganisms able to grow using (sun)light, fertilizers, sugars, CO2, and seawater. They have high potential as a feedstock for food, feed, ...

    Abstract The urge for food security and sustainability has advanced the field of microalgal biotechnology. Microalgae are microorganisms able to grow using (sun)light, fertilizers, sugars, CO2, and seawater. They have high potential as a feedstock for food, feed, energy, and chemicals. Microalgae grow faster and have higher areal productivity than plant crops, without competing for agricultural land and with 100% efficiency uptake of fertilizers. In comparison with bacterial, fungal, and yeast single-cell protein production, based on hydrogen or sugar, microalgae show higher land-use efficiency. New insights are provided regarding the potential of microalgae replacing soy protein, fish oil, and palm oil and being used as cell factories in modern industrial biotechnology to produce designer feed, recombinant proteins, biopharmaceuticals, and vaccines.
    Keywords biofactories ; lipids ; microalgae ; mixotrophy ; photosynthesis ; single-cell protein
    Language English
    Publishing country nl
    Document type Article ; Online
    ZDB-ID 47474-5
    ISSN 1879-3096 ; 0167-7799
    ISSN (online) 1879-3096
    ISSN 0167-7799
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 2750: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

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  9. Article ; Online: Expression of glycerol-3-phosphate acyltransferase increases non-polar lipid accumulation in Nannochloropsis oceanica

    Südfeld, Christian / Kiyani, Aamna / Wefelmeier, Katrin / Wijffels, René H. / Barbosa, Maria J. / D’Adamo, Sarah

    Microbial Cell Factories

    2023  Volume 22

    Abstract: Microalgae are considered a suitable production platform for high-value lipids and oleochemicals. Several species including Nannochloropsis oceanica produce large amounts of essential ω-3 polyunsaturated fatty acids (PUFAs) which are integral components ... ...

    Abstract Microalgae are considered a suitable production platform for high-value lipids and oleochemicals. Several species including Nannochloropsis oceanica produce large amounts of essential ω-3 polyunsaturated fatty acids (PUFAs) which are integral components of food and feed and have been associated with health-promoting effects. N. oceanica can further accumulate high contents of non-polar lipids with chemical properties that render them a potential replacement for plant oils such as palm oil. However, biomass and lipid productivities obtained with microalgae need to be improved to reach commercial feasibility. Genetic engineering can improve biomass and lipid productivities, for instance by increasing carbon flux to lipids. Here, we report the overexpression of glycerol-3-phosphate acyltransferase (GPAT) in N. oceanica during favorable growth conditions as a strategy to increase non-polar lipid content. Transformants overproducing either an endogenous (NoGPAT) or a heterologous (Acutodesmus obliquus GPAT) GPAT enzyme targeted to the endoplasmic reticulum had up to 42% and 51% increased non-polar lipid contents, respectively, compared to the wild type. Biomass productivities of transformant strains were not substantially impaired, resulting in lipid productivities that were increased by up to 37% and 42% for NoGPAT and AoGPAT transformants, respectively. When exposed to nutrient stress, transformants and wild type had similar lipid contents, suggesting that GPAT enzyme exerts strong flux control on lipid synthesis in N. oceanica under favorable growth conditions. NoGPAT transformants further accumulated PUFAs in non-polar lipids, reaching a total of 6.8% PUFAs per biomass, an increase of 24% relative to the wild type. Overall, our results indicate that GPAT is an interesting target for engineering of lipid metabolism in microalgae, in order to improve non-polar lipid and PUFAs accumulation in microalgae.
    Keywords Life Science
    Subject code 571
    Language English
    Publishing country nl
    Document type Article ; Online
    ZDB-ID 2091377-1
    ISSN 1475-2859
    ISSN 1475-2859
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article: Accumulation of medium chain fatty acids in Nannochloropsis oceanica by heterologous expression of Cuphea palustris thioesterase FatB1

    Südfeld, Christian / Kiyani, Aamna / Buckens, Hortense / Hubáček, Michal / Wijffels, René H. / Barbosa, Maria J. / D'Adamo, Sarah

    Algal research. 2022 May, v. 64

    2022  

    Abstract: Medium chain fatty acids (MCFAs) are compounds of considerable commercial interest that have applications in food, feed, and the production of industrially relevant chemicals. Due to their antipathogenic and health-promoting effects, they are further ... ...

    Abstract Medium chain fatty acids (MCFAs) are compounds of considerable commercial interest that have applications in food, feed, and the production of industrially relevant chemicals. Due to their antipathogenic and health-promoting effects, they are further discussed as potential therapeutic agents for disease treatment and infection prevention and control. Domesticated agricultural crops that can grow in temperate climates lack MCFAs, and increased cultivation of tropical MCFA-rich species such as oil palm is associated with deforestation and a decrease in biodiversity. Alternative sources for more sustainably produced MCFAs are non-domesticated plants such as Cuphea spp., as well as oil crops and oleaginous microalgae that can be genetically engineered to accumulate MCFAs by expression of acyl-acyl carrier protein thioesterases (TEs) from MCFA-producing plants. Here, we report the heterologous expression of a TE from Cuphea palustris in the industrially relevant microalga Nannochloropsis oceanica. Using a recently developed gene expression system, we engineered transformant strains that accumulated up to 2.84 and 4.15% of C8:0 and C10:0 in storage lipids, respectively, and we observed no effect on growth. We further show that MCFA accumulation was negatively correlated with total neutral lipid content, suggesting the presence of regulatory mechanisms that limit MCFA accumulation in Nannochloropsis.
    Keywords Cuphea ; Elaeis guineensis ; Nannochloropsis ; biodiversity ; deforestation ; gene expression ; health promotion ; heterologous gene expression ; lipid content ; microalgae ; oils ; research ; therapeutics
    Language English
    Dates of publication 2022-05
    Publishing place Elsevier B.V.
    Document type Article
    ISSN 2211-9264
    DOI 10.1016/j.algal.2022.102665
    Database NAL-Catalogue (AGRICOLA)

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