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  1. Article ; Online: Antagonistic activity of autosimbionts А. viridans, B. subtilis and their probiotic association to conditionally microflora.

    D. A. Stepansky

    Medičnì Perspektivi, Vol 20, Iss 1, Pp 14-

    2015  Volume 21

    Abstract: In this research the data on examination of antagonist qualities of bioassotiantes A. viridans and strain B. subtilis 3 towards pathogenic and opportunistic pathogenic microflora isolated from oropharynx and nasopharynx of children who were in contact ... ...

    Abstract In this research the data on examination of antagonist qualities of bioassotiantes A. viridans and strain B. subtilis 3 towards pathogenic and opportunistic pathogenic microflora isolated from oropharynx and nasopharynx of children who were in contact with patients with pulmonary tuberculosis (MBT +) are submitted. The expressed antagonist activity of autosimbionts A. viridans towards pathogenic and opportunistic pathogenic microflora was shown. Common antagonist activity of A. viridans (k) N 1 and B. subtilis 3 towards diverse strains of test-cultures is 1,5-2 times higher, than separate antagonist activity of A. viridans (k) №1 and B. subtilis 3. Received research data showed the possibility of continuing work on development of probiotic associations, that contain representatives of normal microflora - bioassociants A. viridans and probiotic strains B. subtilis 3 with broadspectrum of antagonistic activity in relation to the various groups of bacterium.
    Keywords autosimbionts ; antagonist activity ; Aerococcus ; Medicine ; R
    Language English
    Publishing date 2015-03-01T00:00:00Z
    Publisher Dnipro State Medical University
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Homo- and heteroadhesion activity of autosymbionts of Aerococcus Viridans strains.

    D. A. Stepansky / G. N. Kremenchutsky / I. P. Koshevaya / Ye. A. Velychko

    Medičnì Perspektivi, Vol 21, Iss 4, Pp 67-

    2016  Volume 71

    Abstract: Adhesion is the major stage of interaction of microorganisms with macroorganism, important factor with the help of which microflora colonizes different biotypes of macroorganism, thus providing colonization resistance, preventing adhesion and pathogenic ... ...

    Abstract Adhesion is the major stage of interaction of microorganisms with macroorganism, important factor with the help of which microflora colonizes different biotypes of macroorganism, thus providing colonization resistance, preventing adhesion and pathogenic invasion. In our study data about adhesive features of Aerococcus viridans autophytes of rats, mice and human are presented. Aerococcus viridans autosymbionts have higher performances of adhesion in the condition of heteroadhesion. Among performance of homoadhesionthe highest microorganisms adhesive index (MAI) was in human autosymbionts – 5,78±0,04, in rats – 4,49±0,06 and mice – 4,29±0,04. On the account of greater adhesive features, using of autosymbionts of Aerococcus viridans theoretically can be justified for correction of disbiotic conditions of a human and animals, this requires further study.
    Keywords autosymbionts ; Aerococcus viridans ; adhesion ; Medicine ; R
    Language English
    Publishing date 2016-12-01T00:00:00Z
    Publisher Dnipro State Medical University
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Biological properties of aerococci and bacilli as a component of new associate-probiotic complex

    S. I. Valchuk / D. A. Stepansky / T. N. Shevchenko / I. P. Koshevaya / S. A. Ryzhenko / G. N. Kremenchutsky

    Vìsnik Dnìpropetrovsʹkogo Unìversitetu. Serìâ Bìologìâ, Medicina, Vol 6, Iss 1, Pp 57-

    2015  Volume 62

    Abstract: Dysbioses of the gastrointestinal tract are common among people of all ages and genders. Development of this pathology is associated with a number of complications, from indigestion to occurrence of malignant disease. Therefore, there is a need in ... ...

    Abstract Dysbioses of the gastrointestinal tract are common among people of all ages and genders. Development of this pathology is associated with a number of complications, from indigestion to occurrence of malignant disease. Therefore, there is a need in development of measures of their prevention and correction. Probiotics are used as drugs against dysbiosis. Most of the presently known probiotics contain bacterial cells of one species, although combination preparations feature higher efficiency. At the same time, there are difficulties in construction of these drugs, primarily due to incompatibility of physiological properties of microorganisms and mutually antagonistic action of their components. The aim was to examine the compatibility of Bacillus subtilis and Aerococcus viridans in a single preparation, their antagonistic activity against different strains of test-cultures and general antagonism directed on different groups of bacteria for subsequent formation of associative probiotic complex. Properties of aerococci strains were studied and A. viridans 167 strain was selected for inclusion into the probiotic preparation. The tested strain showed the highest indicators of production of hydrogen peroxide, which is one of the mechanisms of antagonistic effect against opportunistic pathogens. General study of biological properties of aerococci strains showed that producing of hydrogen peroxide and superoxide radical in them was conditioned by functioning of NAD-independent lactatoxidase. It has been determined that antioxidant defense of aerococci from the action of endogenous and active excretable forms of oxygen was provided by activity of superoxide-dismutase and GSH-peroxidase. The method of deferred antagonism found no depressing mutual action between probiotic strains of B. subtilis 3 and A. viridans 167 at their joint cultivation. Inhibition of growth at the joint application of A. viridans 167 and B. subtilis 3 strains was recorded for both museum and clinical strains of test-cultures Escherichia coli, Proteus vulgaris, Klebsiella ozaenae, Citrobacter freundii, Pseudomonas aeruginosa, Staphylococcus aureus, S. epidermidis, Candida albicans. Separate application of A. viridans 167 or B. subtilis 3 against strains of these opportunistic pathogens was characterized by relatively less antagonistic effect of each of strains under study. The results allow us to recommend the studied strains of B. subtilis 3 and A. viridans 167 for use as the components to construct a new associative probiotic preparation.
    Keywords probiotics ; Bacillus subtilis 3 ; Aerococcus viridans 167 ; antagonism ; dysbiosis ; Science ; Q
    Subject code 630
    Language English
    Publishing date 2015-03-01T00:00:00Z
    Publisher Oles Honchar Dnipropetrovsk National University
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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