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Article ; Online: Performance evaluation of UF-4000 body fluid mode for automated body fluid cell counting.

Siatkowski, Marie / Dahyot, Sandrine / Pestel-Caron, Martine / Boyer, Sophie

Clinica chimica acta; international journal of clinical chemistry

2022 Volume 531, Page(s) 152–156

Abstract: Background: Cytological analysis of body fluids (BF) provides important information for diagnosis in various medical conditions. We evaluated the analytical performance of the UF-4000 BF mode for ascitic, cerebrospinal, pleural, synovial and continuous ... ...

Abstract	Background: Cytological analysis of body fluids (BF) provides important information for diagnosis in various medical conditions. We evaluated the analytical performance of the UF-4000 BF mode for ascitic, cerebrospinal, pleural, synovial and continuous ambulatory peritoneal dialysis fluids compared to light microscopy counting (LM). Materials and methods: 223 consecutive BF were analyzed by UF-4000 and results were compared using Pearson's correlation, Bland-Altman analysis, and contingence tests at relevant cut-off values. This study also included the evaluation of precision, linearity, and carryover. Results: For white and red blood cells (WBC, RBC) counts in all BF, correlation was excellent with Pearson's coefficients R Conclusion: UF-4000 BF mode is a good alternative to manual LM for BF cell counting. This automated method gives rapid and accurate results which is important for therapeutic decisions.
MeSH term(s)	Body Fluids ; Erythrocyte Count/methods ; Erythrocytes ; Humans ; Leukocyte Count ; Microscopy/methods ; Reproducibility of Results
Language	English
Publishing date	2022-04-06
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	80228-1
ISSN	1873-3492 ; 0009-8981
ISSN (online)	1873-3492
ISSN	0009-8981
DOI	10.1016/j.cca.2022.04.002
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Article ; Online: Performance evaluation of UF-4000 body fluid mode for detection of bacteria in body fluids.

Siatkowski, Marie / Dahyot, Sandrine / Pestel-Caron, Martine / Boyer, Sophie

Clinica chimica acta; international journal of clinical chemistry

2022 Volume 538, Page(s) 9–14

Abstract: Background: Microbiological analysis of body fluids (BF) provides important information for diagnosis of infection. We evaluated the analytical performance of bacterial count by UF-4000 BF mode for ascitic, cerebrospinal, pleural, synovial and ... ...

Abstract	Background: Microbiological analysis of body fluids (BF) provides important information for diagnosis of infection. We evaluated the analytical performance of bacterial count by UF-4000 BF mode for ascitic, cerebrospinal, pleural, synovial and continuous ambulatory peritoneal dialysis fluids compared to classical microbiological procedure (direct Gram staining and culture). Materials and methods: For the 1,734 BF analyzed, distribution of UF-4000 bacterial count was analyzed according to the level of growth culture and results were compared using Mann-Whitney test. ROC curves analysis allowed to define the best cut-off value to predict or exclude positive culture for each type of BF. Results: UF-4000 bacterial counts were significantly lower in sterile than in infected BFs (p < 0.00001) and correlated with the level of growth on culture. The ROC curves of bacteria/μL and culture positivity yielded area under the curve >0.80 for each type of BF. Optimal cut-offs were chosen with excellent statistical parameters (sensitivity ranging from 0.70 to 0.86, specificity from 0.78 to 0.98, negative predictive value >0.95 and Youden index >0.55). Conclusion: For BF, UF-4000 bacterial count correlate with culture results and is a discriminative method enhancing detection of microbiological etiology. It could be used as a screening method based on the cut-off values proposed in this study.
Language	English
Publishing date	2022-10-29
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	80228-1
ISSN	1873-3492 ; 0009-8981
ISSN (online)	1873-3492
ISSN	0009-8981
DOI	10.1016/j.cca.2022.10.020
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Article ; Online: Impact of clonal lineages on susceptibility of Staphylococcus lugdunensis to chlorhexidine digluconate and chloride benzalkonium.

Destruel, Laurie / Lecomte, Marine / Grand, Maxime / Leoz, Marie / Pestel-Caron, Martine / Dahyot, Sandrine

BMC microbiology

2023 Volume 23, Issue 1, Page(s) 337

Abstract: Background: Little is known about susceptibility of Staphylococcus lugdunensis to antiseptics. The objective of this study was to evaluate, at the molecular and phenotypic level, the susceptibility of 49 clinical S. lugdunensis strains (belonging to the ...

Abstract	Background: Little is known about susceptibility of Staphylococcus lugdunensis to antiseptics. The objective of this study was to evaluate, at the molecular and phenotypic level, the susceptibility of 49 clinical S. lugdunensis strains (belonging to the seven clonal complexes [CCs] defined by multilocus sequence typing) to two antiseptics frequently used in healthcare settings (chlorhexidine digluconate [CHX] and chloride benzalkonium [BAC]). Results: The minimum inhibitory concentrations (MICs), by broth microdilution method, varied for BAC from 0.25 mg/L to 8 mg/L (MIC Conclusion: The norA gene was detected in all the strains, suggesting that it could belong to the core genome of S. lugdunensis. S. lugdunensis is highly susceptible to both antiseptics tested. Reduced susceptibility to BAC and CHX was a rare phenomenon. Of note, a tendency to higher MICs of BAC was detected for CC3 isolates. These results should be confirmed on a larger collection of strains.
MeSH term(s)	Benzalkonium Compounds/pharmacology ; Staphylococcus lugdunensis/genetics ; Chlorides ; Bacterial Proteins/genetics ; Chlorhexidine/pharmacology ; Anti-Infective Agents, Local/pharmacology ; Microbial Sensitivity Tests ; Disinfectants/pharmacology
Chemical Substances	chlorhexidine gluconate (MOR84MUD8E) ; Benzalkonium Compounds ; Chlorides ; Bacterial Proteins ; Chlorhexidine (R4KO0DY52L) ; Anti-Infective Agents, Local ; Disinfectants
Language	English
Publishing date	2023-11-13
Publishing country	England
Document type	Journal Article
ZDB-ID	2041505-9
ISSN	1471-2180 ; 1471-2180
ISSN (online)	1471-2180
ISSN	1471-2180
DOI	10.1186/s12866-023-03088-1
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Article ; Online: Contribution of Antibiotic Susceptibility Testing and CH Typing Compared to Next-Generation Sequencing for the Diagnosis of Recurrent Urinary Tract Infections Due to Genetically Identical Escherichia coli Isolates: a Prospective Cohort Study of Cystitis in Women.

Vautrin, Nicolas / Alexandre, Kévin / Pestel-Caron, Martine / Bernard, Estelle / Fabre, Roland / Leoz, Marie / Dahyot, Sandrine / Caron, François

Microbiology spectrum

2023 Volume 11, Issue 4, Page(s) e0278522

Abstract: Recurrent cystitis is a common disease in women, mainly due to uropathogenic Escherichia coli (UPEC). For decades, typing methods now considered obsolete suggested that relapse by the same clone is dominant over reinfection, most UPEC strains being ... ...

Abstract	Recurrent cystitis is a common disease in women, mainly due to uropathogenic Escherichia coli (UPEC). For decades, typing methods now considered obsolete suggested that relapse by the same clone is dominant over reinfection, most UPEC strains being otherwise fully susceptible to antibiotics. We aimed to update these data. Thanks to a prospective study over 17 months, we recruited 323 women with cystitis. Of these, 251 of them had sporadic infection and 72 had recurrence, with 2 to 9 episodes per patient for a total of 131 UPEC isolates and 145 UPEC pairs at patient level. Phylogroups B2 (52.4%) and D (14.1%) were overall dominant, as expected due to their particular urovirulence. CH typing identified 119 distinct profiles with no CH type particularly associated with recurrence. Relapse was attested by CH typing for only 30.6% (22 out of 72), with very diverse situations ranging from all episodes due to the same clone to alternating reinfections and relapses. Next-generation sequencing confirmed the clonality for all but two of the 145 UPEC pairs. Antibiotic resistance was common for recurrent cystitis isolates (only 25 [17.2%] out of 145 UPEC pairs were fully susceptible), allowing us to predict UPEC clonality. Indeed, antibiotic susceptibility profile matched CH typing for 104 (71.7%) pairs. Finally, we demonstrated a large genetic diversity among UPEC isolates responsible for cystitis in women, even in cases of recurrence for which reinfection appeared dominant over relapse. Recurrent cystitis appears to be a heterogeneous disease requiring tailored treatment and prevention.
MeSH term(s)	Humans ; Female ; Prospective Studies ; Reinfection ; Escherichia coli Infections/drug therapy ; Escherichia coli Infections/diagnosis ; Urinary Tract Infections/diagnosis ; Cystitis/diagnosis ; Disease Susceptibility ; Recurrence ; Uropathogenic Escherichia coli/genetics ; Anti-Bacterial Agents/pharmacology ; Virulence Factors/genetics ; High-Throughput Nucleotide Sequencing
Chemical Substances	Anti-Bacterial Agents ; Virulence Factors
Language	English
Publishing date	2023-07-11
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2807133-5
ISSN	2165-0497 ; 2165-0497
ISSN (online)	2165-0497
ISSN	2165-0497
DOI	10.1128/spectrum.02785-22
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Article ; Online: Phenotypic and genotypic within-host diversity of Pseudomonas aeruginosa urinary isolates.

Cottalorda, Agnès / Dahyot, Sandrine / Soares, Anaïs / Alexandre, Kevin / Zorgniotti, Isabelle / Etienne, Manuel / Jumas-Bilak, Estelle / Pestel-Caron, Martine

Scientific reports

2022 Volume 12, Issue 1, Page(s) 5421

Abstract: This study aimed to assess phenotypic and molecular inter-patient and within-host diversity of Pseudomonas aeruginosa isolates responsible for urinary tract infection (UTI) or asymptomatic bacteriuria (AB). Clinical data of 120 consecutive P. aeruginosa ... ...

Abstract	This study aimed to assess phenotypic and molecular inter-patient and within-host diversity of Pseudomonas aeruginosa isolates responsible for urinary tract infection (UTI) or asymptomatic bacteriuria (AB). Clinical data of 120 consecutive P. aeruginosa UTI (n = 40) and AB (n = 80) were prospectively analyzed. Up to five P. aeruginosa isolates per sample were collected. Antimicrobial susceptibility testing (AST) was determined for all isolates (n = 591); a subset of 358 was characterized by multilocus sequence typing. 444 isolates (75%) were non-multidrug resistant (MDR), 113 (19%) were MDR, and 34 (6%) were extensively drug resistant. A genetically highly diverse population was observed (64 sequence types [STs]), without strict correlation between genotypes and clinical settings. 35 patients (28%; 12 UTIs and 23 ABs) presented distinct antimicrobial resistance (AMR) profiles within a given urine sample, significantly associated with previous carbapenem and fluroquinolones exposure; five of them also exhibited polyclonal UTI or AB (with isolates belonging to two STs). P. aeruginosa urinary isolates of these 120 patients were highly diverse, in terms of AMR as well as genetic background. Both within-host AMR and molecular diversity can complicate AST, treatment and control of P. aeruginosa UTI.
MeSH term(s)	Drug Resistance, Multiple, Bacterial/genetics ; Genotype ; Humans ; Microbial Sensitivity Tests ; Pseudomonas Infections/drug therapy ; Pseudomonas Infections/epidemiology ; Pseudomonas aeruginosa/genetics
Language	English
Publishing date	2022-03-30
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-022-09234-5
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Article ; Online: Cellulitis and bacteremia after a snake bite despite antibiotic preemptive treatment: A diagnosis challenge.

Réveillon-Istin, Mathilde / Resiere, Dabor / Dahyot, Sandrine / Alexandre, Kévin / Mehdaoui, Hossein / Caron, François

Travel medicine and infectious disease

2020 Volume 35, Page(s) 101555

MeSH term(s)	Adult ; Animals ; Anti-Bacterial Agents/therapeutic use ; Antivenins/administration & dosage ; Bacteremia/drug therapy ; Bothrops ; Cellulitis/drug therapy ; Enterobacteriaceae Infections/drug therapy ; Humans ; Male ; Morganella morganii/isolation & purification ; Snake Bites/complications
Chemical Substances	Anti-Bacterial Agents ; Antivenins
Language	English
Publishing date	2020-01-11
Publishing country	Netherlands
Document type	Case Reports ; Journal Article
ZDB-ID	2170891-5
ISSN	1873-0442 ; 1477-8939
ISSN (online)	1873-0442
ISSN	1477-8939
DOI	10.1016/j.tmaid.2020.101555
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Article: Are

Vautrin, Nicolas / Dahyot, Sandrine / Leoz, Marie / Caron, François / Grand, Maxime / Feldmann, Audrey / Gravey, François / Legris, Stéphanie / Ribet, David / Alexandre, Kévin / Pestel-Caron, Martine

bioRxiv : the preprint server for biology

2023

Abstract: Specific determinants associated with ... ...

Abstract	Specific determinants associated with Uropathogenic
Language	English
Publishing date	2023-11-09
Publishing country	United States
Document type	Preprint
DOI	10.1101/2023.11.08.566351
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Article ; Online: Hydrolysis spectrum extension of CMY-2-like β-lactamases resulting from structural alteration in the Y-X-N loop.

Dahyot, Sandrine / Mammeri, Hedi

Antimicrobial agents and chemotherapy

2012 Volume 56, Issue 3, Page(s) 1151–1156

Abstract: The Citrobacter freundii isolate CHA, which was responsible for postoperative peritonitis after 10 days of cefepime therapy, displayed a phenotype of resistance consistent with extended-spectrum AmpC (ESAC) β-lactamase. The chromosome-borne bla(AmpC-CHA) ...

Abstract	The Citrobacter freundii isolate CHA, which was responsible for postoperative peritonitis after 10 days of cefepime therapy, displayed a phenotype of resistance consistent with extended-spectrum AmpC (ESAC) β-lactamase. The chromosome-borne bla(AmpC-CHA) gene was amplified and sequenced, revealing five amino acid substitutions, I125V, R148H, Q196H, V305A, and V348A, in the product compared to the sequence of native AmpC. A cloning experiment yielded the Escherichia coli TOP10(pAmpC-CHA) strain, which was resistant to all extended-spectrum cephalosporins (ESCs), including cefepime. To ascertain whether the R148H substitution accounted for the hydrolysis spectrum extension, it was reverted by site-directed mutagenesis. The resulting E. coli TOP10(pAmpC-CHA-H148R) strain was fully susceptible to cefepime, thus confirming that the Arg-148 replacement was mandatory for substrate profile enlargement. To further characterize the phenotypical and biochemical effects induced by the R148H change, it was introduced by site-directed mutagenesis into the CMY-2 β-lactamase, which is structurally related to the chromosome-borne cephalosporinase of C. freundii. The CMY-2-R148H variant conferred increased MICs of ESCs, whereas those of carbapenems were unchanged even in a porin-deficient E. coli strain. Moreover, it exhibited increased catalytic efficiency (k(cat)/K(m)) toward ceftazidime (100-fold) due to an enhanced hydrolysis rate (k(cat)), whereas the enzymatic parameters toward imipenem were unchanged. The structural analysis of the AmpC variant showed that the R148H replacement occurred in the loop containing the Y-X-N motif, which is the counterpart of the SDN loop in class A β-lactamases. This study shows that the Y-X-N loop is a novel hot spot for mutations accounting for hydrolysis spectrum extension in CMY-2-type enzymes.
MeSH term(s)	Adult ; Amino Acid Substitution ; Anti-Bacterial Agents/administration & dosage ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Carbapenems/administration & dosage ; Cephalosporin Resistance/genetics ; Cephalosporins/administration & dosage ; Citrobacter freundii/enzymology ; Citrobacter freundii/genetics ; Citrobacter freundii/pathogenicity ; Cloning, Molecular ; Enterobacteriaceae Infections/drug therapy ; Enterobacteriaceae Infections/microbiology ; Escherichia coli/enzymology ; Escherichia coli/genetics ; Humans ; Hydrolysis ; Kinetics ; Male ; Microbial Sensitivity Tests ; Models, Molecular ; Mutagenesis, Site-Directed ; Peritonitis/drug therapy ; Peritonitis/microbiology ; Postoperative Complications/drug therapy ; Postoperative Complications/microbiology ; Protein Structure, Tertiary ; beta-Lactamases/genetics ; beta-Lactamases/metabolism
Chemical Substances	Anti-Bacterial Agents ; Bacterial Proteins ; Carbapenems ; Cephalosporins ; beta-lactamase CMY-2 (EC 3.5.2.-) ; beta-Lactamases (EC 3.5.2.6)
Language	English
Publishing date	2012-01-09
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	217602-6
ISSN	1098-6596 ; 0066-4804
ISSN (online)	1098-6596
ISSN	0066-4804
DOI	10.1128/AAC.05630-11
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Zs.A 809: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Efficacy of temocillin against MDR Enterobacterales: a retrospective cohort study.

Alexandre, Kévin / Leysour de Rohello, François / Dahyot, Sandrine / Etienne, Manuel / Tiret, Isabelle / Gillibert, André / Pestel-Caron, Martine / Caron, François

The Journal of antimicrobial chemotherapy

2020 Volume 76, Issue 3, Page(s) 784–788

Abstract: Objectives: EUCAST recently advised against temocillin use, except for non-serious urinary tract infections (UTI) caused by Escherichia coli, Klebsiella spp. (except Klebsiella aerogenes) and Proteus mirabilis (EKP) treated with a dose of 2 g q8h. We ... ...

Abstract	Objectives: EUCAST recently advised against temocillin use, except for non-serious urinary tract infections (UTI) caused by Escherichia coli, Klebsiella spp. (except Klebsiella aerogenes) and Proteus mirabilis (EKP) treated with a dose of 2 g q8h. We aimed to analyse our practice in the context of a larger temocillin use in France. Patients and methods: All ≥3 day temocillin prescriptions from 2016 to 2019 were reviewed, with reference to French recommendations and a susceptibility breakpoint of 8 mg/L. The primary outcome was early clinical failure (antibiotic switch, relapse or death within 10 days after the completion of antibiotic treatment). Results: Overall, 153 cases were analysed: 123 cases of UTI (80.4%) and 133 cases of monomicrobial infection with Enterobacterales (86.9%). A total of 160 Enterobacterales were isolated, comprising 108 (67.5%) ESBL producers and 30 (20.7%) non-EKP species. The rate of early clinical failure was 9.2% and was significantly lower for UTI compared with non-UTI (4.9% versus 26.7%, P = 0.001) and for sepsis compared with severe sepsis or septic shock (6.2% versus 25%, P = 0.011). It was not different between 2 g q12h and 2 g q8h doses (10% versus 7.4%, P = 0.81) and between EKP and other Enterobacterales (8.7% versus 14.3%, P = 0.41). Conclusions: EUCAST recommendations on urinary isolates seem to be too restrictive. Our data support the efficacy of temocillin at a dose of 2 g q12h to treat patients with non-severe complicated UTI caused by MDR Enterobacterales with an MIC of ≤8 mg/L, whatever the species.
MeSH term(s)	Anti-Bacterial Agents/therapeutic use ; France ; Humans ; Microbial Sensitivity Tests ; Penicillins ; Retrospective Studies ; Urinary Tract Infections/drug therapy
Chemical Substances	Anti-Bacterial Agents ; Penicillins ; temocillin (03QB156W6I)
Language	English
Publishing date	2020-11-27
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	191709-2
ISSN	1460-2091 ; 0305-7453
ISSN (online)	1460-2091
ISSN	0305-7453
DOI	10.1093/jac/dkaa486
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Zs.A 1197: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article: Within-Host Microevolution of

Cottalorda, Agnès / Leoz, Marie / Dahyot, Sandrine / Gravey, François / Grand, Maxime / Froidure, Thomas / Aujoulat, Fabien / Le Hello, Simon / Jumas-Bilak, Estelle / Pestel-Caron, Martine

Frontiers in microbiology

2021 Volume 11, Page(s) 611246

Abstract: Background: Pseudomonas aeruginosa: Results: Whole genome sequencing was performed on 108 : Conclusion: This study provides the first insight ... ...

Abstract	Background: Pseudomonas aeruginosa Results: Whole genome sequencing was performed on 108 Conclusion: This study provides the first insight into
Language	English
Publishing date	2021-01-14
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2020.611246
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