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  1. Article: [Analysis of FBN1 genemutations in a pedigree with Marfan syndrome].

    Zheng, Q / Li, K L / Dai, G L / Xiong, D / Yao, M Y / Chen, X / Li, Y M / Zhang, Y Y / Li, H R / Cao, Y

    Zhonghua yi xue za zhi

    2022  Volume 102, Issue 34, Page(s) 2702–2706

    Abstract: Mutations in fibrillin-1 (FBN1) were detected in an autosomal dominant Marfan syndrome (MFS) pedigree. The related phenotypes and the significance of mutation screening were discussed. Complete medical and cardiovascular examinations for all pedigree ... ...

    Abstract Mutations in fibrillin-1 (FBN1) were detected in an autosomal dominant Marfan syndrome (MFS) pedigree. The related phenotypes and the significance of mutation screening were discussed. Complete medical and cardiovascular examinations for all pedigree members were performed. Whole exons sequencing (WES) was used to sequence the DNA of the patients and their relatives. The potential pathogenic mutation sites were screened by bioinformatics method. Sanger sequencing was used to verify the mutation sites in the pedigree. The results showed that FBN1 missense mutation was c.6806 T>C in exon 56, resulting in isoleucine being replaced by threonine (p. Ile2269Thr). This mutation has not been reported in Chinese Han population. The occurrence of the mutations strongly correlated with the phenotypes of the patients. The results expand the mutation spectrum of FBN1, and it is helpful to further explore the molecular pathogenesis of MFS and MFS related diseases.
    MeSH term(s) Exons ; Fibrillin-1/genetics ; Humans ; Marfan Syndrome/diagnosis ; Marfan Syndrome/genetics ; Marfan Syndrome/pathology ; Mutation, Missense ; Pedigree
    Chemical Substances FBN1 protein, human ; Fibrillin-1
    Language Chinese
    Publishing date 2022-09-12
    Publishing country China
    Document type Journal Article
    ZDB-ID 132513-9
    ISSN 0376-2491
    ISSN 0376-2491
    DOI 10.3760/cma.j.cn112137-20220531-01200
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Sensitive and selective LC-MS/MS assay for quantitation of flutrimazole in human plasma.

    Shen, L / Liu, S-J / Zhang, N-S / Dai, G-L / Zou, C / Li, C-Y / Chen, X-H / Ju, W-Z

    European review for medical and pharmacological sciences

    2017  Volume 21, Issue 12, Page(s) 2964–2969

    Abstract: Objective: A highly sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for the determination of flutrimazole in human plasma. This study was to investigate the application of sensitive and selective LC-MS/MS ... ...

    Abstract Objective: A highly sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for the determination of flutrimazole in human plasma. This study was to investigate the application of sensitive and selective LC-MS/MS method for quantitation of flutrimazole in human plasma.
    Materials and methods: The analysis and internal standard were extracted with ether and hexane (v:v, 1:1) followed by a rapid isocratic elution with a 0.1% formic acid/methanol (v:v, 20:80) on a C18 column (50 mm × 2.1 mm I.D.) and subsequent analysis by mass spectrometry in the multi-reaction-monitoring mode. The precursor to production transitions of m/z 279.0 → 183.1 and m/z 441.0 → 295.1 were used to measure the analyte and the internal standard.
    Results: The assay was linear over the concentration range of 0.996-99.6 ng•mL-1 for flutrimazole in human plasma. The lower limit of quantification was 0.996 ng•mL-1 and the extraction recovery was larger than 78.83% for flutrimazole. The inter- and intra-day precision of the method at three concentrations was less than 9.26%.
    Conclusions: The LC-MS/MS method was firstly applied to quantitation of flutrimazole in human plasma.
    MeSH term(s) Antifungal Agents/blood ; Biological Assay ; Chromatography, Liquid/methods ; Clotrimazole/analogs & derivatives ; Clotrimazole/blood ; Humans ; Reproducibility of Results ; Sensitivity and Specificity ; Tandem Mass Spectrometry/methods
    Chemical Substances Antifungal Agents ; flutrimazole (776S0UP252) ; Clotrimazole (G07GZ97H65)
    Language English
    Publishing date 2017-07-05
    Publishing country Italy
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 605550-3
    ISSN 2284-0729 ; 1128-3602 ; 0392-291X
    ISSN (online) 2284-0729
    ISSN 1128-3602 ; 0392-291X
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 1887: Show issues Location:
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  3. Article: [Factors influencing poor prognosis of mechanical thrombectomy in time window of acute ischemic stroke].

    Zhang, M / Cui, Q K / Lin, K / Hao, J H / Wang, Z D / Chen, W H / Wen, C M / Dai, G L / Wang, J Y / Liu, W D / Wang, S L / Zhang, L Y

    Zhonghua yi xue za zhi

    2019  Volume 99, Issue 25, Page(s) 1976–1980

    Abstract: Objective: ...

    Abstract Objective:
    MeSH term(s) Brain Ischemia ; Humans ; Prognosis ; Retrospective Studies ; Stroke ; Thrombectomy ; Treatment Outcome
    Language Chinese
    Publishing date 2019-07-03
    Publishing country China
    Document type Journal Article
    ZDB-ID 132513-9
    ISSN 0376-2491
    ISSN 0376-2491
    DOI 10.3760/cma.j.issn.0376-2491.2019.25.015
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Preliminary fluorimetric screening of fourteen palladium complexes as potential antitumor agents.

    Lin, H X / Li, Z L / Dai, G L / Bi, Q S / Yu, R Q

    Science in China. Series B, Chemistry, life sciences & earth sciences

    1993  Volume 36, Issue 10, Page(s) 1216–1223

    Abstract: Making use of the fact that the combination of a drug substance with DNA may inhibit the duplication, synthesis and proliferation of DNA and the consistency of the in vivo and in vitro interactions, the authors worked out a preliminary screening method ... ...

    Abstract Making use of the fact that the combination of a drug substance with DNA may inhibit the duplication, synthesis and proliferation of DNA and the consistency of the in vivo and in vitro interactions, the authors worked out a preliminary screening method for testing complex agents as potential antitumor drugs using ethidium bromide as a fluorescence probe. In this report, the method was applied for in vitro testing fourteen synthesized palladium(II)/phenanthroline/amino acid/chloride complexes as potential non-platinum antitumor agents. The fluorimetric screening method was compared with methylene blue tube test and trypan blue dye exclusion assay. All three methods gave agreeable results. Among the complexes tested, [Pd(phen)(lys)]Cl, [Pd(phen)(arg)]Cl and [Pd(phen)(pro)]Cl showed antineoplastic ratios for animal tumor S-180 56%, 50% and 48%, respectively, in accordance with the order of their binding constants with DNA, 7.96 x 10(6), 4.52 x 10(6) and 1.0 x 10(6), respectively. The test results show that fluorimetric method is simple, cheap and rapid, suitable for preliminary screening of antitumor complexes.
    MeSH term(s) Animals ; Antineoplastic Agents/pharmacology ; Antineoplastic Agents/therapeutic use ; Drug Screening Assays, Antitumor ; Female ; Male ; Mice ; Neoplasm Transplantation ; Organometallic Compounds/pharmacology ; Organometallic Compounds/therapeutic use ; Palladium/pharmacology ; Palladium/therapeutic use ; Sarcoma 180/drug therapy
    Chemical Substances Antineoplastic Agents ; Organometallic Compounds ; Palladium (5TWQ1V240M)
    Language English
    Publishing date 1993-10
    Publishing country China
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 406604-2
    ISSN 1001-652X ; 0253-5823
    ISSN 1001-652X ; 0253-5823
    Database MEDical Literature Analysis and Retrieval System OnLINE

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