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  1. Article: [Influence of family with sequence similarity 134, member B-mediated reticulophagy on lipopolysaccharide-induced apoptosis of mouse dendritic cells].

    Duan, Y / Yao, R Q / Zheng, L Y / Dong, N / Wu, Y / Yao, Y M / Dai, X G

    Zhonghua shao shang yu chuang mian xiu fu za zhi

    2023  Volume 39, Issue 9, Page(s) 857–866

    Abstract: Objective: ...

    Abstract Objective:
    MeSH term(s) Animals ; Mice ; Apoptosis ; Autophagy ; bcl-2-Associated X Protein ; Caspase 3 ; Dendritic Cells/pathology ; Lipopolysaccharides/pharmacology ; RNA, Small Interfering
    Chemical Substances bcl-2-Associated X Protein ; Caspase 3 (EC 3.4.22.-) ; Lipopolysaccharides ; RNA, Small Interfering
    Language Chinese
    Publishing date 2023-10-05
    Publishing country China
    Document type English Abstract ; Journal Article
    ISSN 2097-1109
    ISSN 2097-1109
    DOI 10.3760/cma.j.cn501225-20230227-00063
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: High frequency plant regeneration from cotyledon and hypocotyl explants of ornamental kale

    Dai, X. G / Shi, X. P / Ye, Y. M / Fu, Q / Bao, M. Z

    Biologia plantarum. 2009 Dec., v. 53, no. 4

    2009  

    Abstract: A high frequency shoot regeneration system for ornamental kale [Brassica oleracea L. var. acephala (D.C.) Alef.] was firstly established from seedling cotyledon and hypocotyl explants. The ability of cotyledon and hypocotyl to produce adventitious shoots ...

    Abstract A high frequency shoot regeneration system for ornamental kale [Brassica oleracea L. var. acephala (D.C.) Alef.] was firstly established from seedling cotyledon and hypocotyl explants. The ability of cotyledon and hypocotyl to produce adventitious shoots varied depending upon genotype, seedling age and culture medium. The maximum shoot regeneration frequency was obtained when the explants from cv. Nagoya 4-d-old seedlings were cultured on Murashige and Skoog (MS) medium supplemented with 3 mg dm⁻³ 6-benzylaminopurine (BA) and 0.1 mg dm⁻³ naphthaleneacetic acid (NAA). The frequency of shoot regeneration was 65.0 % for cotyledons, 76.1 % for hypocotyls; and the number of shoots per explant was 4.3 for cotyledons, 8.2 for hypocotyls. Hypocotyl explants were found to be more responsive for regeneration when compared with cotyledons. Among the 4 cultivars tested, Nagoya showed the best shoot regeneration response. The addition of 3.0 mg dm⁻³ AgNO₃ was beneficial to shoot regeneration. Roots were formed on the base of the shoots when cultured on half-strength MS medium.
    Keywords genotype ; rooting
    Language English
    Dates of publication 2009-12
    Size p. 769-773.
    Publisher Springer Netherlands
    Publishing place Dordrecht
    Document type Article
    ZDB-ID 1496498-3
    ISSN 1573-8264 ; 0006-3134
    ISSN (online) 1573-8264
    ISSN 0006-3134
    DOI 10.1007/s10535-009-0141-9
    Database NAL-Catalogue (AGRICOLA)

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  3. Article: Improvement of isolated microspore culture of ornamental kale (Brassica oleracea var. acephala): effects of sucrose concentration, medium replacement, and cold pre-treatment

    Dai, X. G / M. Z. Bao / Q. Fu / X. P. Shi

    Journal of horticultural science & biotechnology. 2009 Sept., v. 84, no. 5

    2009  

    Abstract: By evaluating the effects of the culture medium and cold pre-treatment on microspore embryogenesis, we have developed an efficient and reliable protocol for microspore culture and doubled-haploid plant regeneration of ornamental kale (Brassica oleracea ...

    Abstract By evaluating the effects of the culture medium and cold pre-treatment on microspore embryogenesis, we have developed an efficient and reliable protocol for microspore culture and doubled-haploid plant regeneration of ornamental kale (Brassica oleracea var. acephala). Microspore embryogenesis at high frequency was obtained using a liquid NLN medium containing 16% (w/v) sucrose (NLN-16) without medium replacement. Of the genotypes tested, the cultivar ‘Peachy Dancing’ produced the highest yield of embryos (averaging 123.63 embryos per dish). Medium replacement was found to deplete embryo yield significantly in all three genotypes tested (namely, ‘Nagoya’, ‘Peachy Dancing’, and ‘P3’), when compared with continuous culture of microspores in the original incubation medium.A 48-h cold pre-treatment, delivered to the flower buds of ‘Nagoya’ and ‘P3’ before microspore isolation, significantly enhanced subsequent microspore embryogenesis; however, cold pre-treatment of flower buds had a negative effect on ‘Peachy Dancing’. Cotyledonary embryos were incubated on B5 basal medium and abnormal embryos on B5 medium containing 2% (w/v) sucrose, 1.5 mg l–1 6-benzylaminopurine, and 0.25 mg l–1 naphthaleneacetic acid. In each case, high quality embryos were regenerated that went on to produce healthy, vigorous plants.There were 38 – 50% spontaneous diploid, microspore-derived plants among the three genotypes tested.
    Keywords benzyladenine ; biotechnology ; Brassica oleracea var. sabellica ; buds ; cultivars ; culture media ; diploidy ; doubled haploids ; embryogenesis ; genotype ; horticulture ; kale ; liquids ; microspores ; naphthaleneacetic acid ; protocols ; sucrose
    Language English
    Dates of publication 2009-09
    Size p. 519-525.
    Publishing place Headley Brothers Ltd.
    Document type Article
    Note Includes references
    ZDB-ID 1416403-6
    ISSN 2380-4084 ; 1462-0316
    ISSN (online) 2380-4084
    ISSN 1462-0316
    DOI 10.1080/14620316.2009.11512559
    Database NAL-Catalogue (AGRICOLA)

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  4. Article: Effects of Lu-Duo-Wei capsules on superoxide dismutase activity and contents of malondialdehyde and lipofuscin in the brain of the housefly.

    Cui, X / Dai, X G / Li, W B / Zhang, B L / Fang, Y Z

    The American journal of Chinese medicine

    2000  Volume 28, Issue 2, Page(s) 259–262

    Abstract: The biochemical actions of Lu-Duo-Wei capsules and tea polyphenol in relation to their antioxidant capability have been compared in the male housefly, Musca domestica. It was found that tea polyphenol had the effect of increasing the activity of ... ...

    Abstract The biochemical actions of Lu-Duo-Wei capsules and tea polyphenol in relation to their antioxidant capability have been compared in the male housefly, Musca domestica. It was found that tea polyphenol had the effect of increasing the activity of superoxide dismutase (SOD) in the brain of the housefly only at the 60th day in the experimental period whereas Lu-Duo-Wei showed more obvious effects on SOD than tea polyphenol irrespective of experimental days. Moreover, the contents of malondialdehyde (MDA) and the rate of lipofuscin accumulation were decreased by the effect of Lu-Duo-Wei at the 20th, 40th, 50th and 60th days, while the similar effect of tea polyphenol on the content of MDA and lipofuscin was observed only at the 40th day. These results indicated that the biochemical actions of Lu-Duo-Wei in antiaging was much stronger than those of tea polyphenol, the mechanism of which was probably related to the synergistic effect of its involved antioxidants and others as well as the exclusive promotion of SOD biosynthesis/or a reduction of free radical-induced damage of the enzyme.
    MeSH term(s) Analysis of Variance ; Animals ; Brain/drug effects ; Brain/enzymology ; Brain/metabolism ; Capsules ; Drugs, Chinese Herbal/pharmacology ; Flavonoids ; Houseflies ; Lipofuscin/metabolism ; Male ; Malondialdehyde/metabolism ; Phenols/pharmacology ; Polymers/pharmacology ; Polyphenols ; Superoxide Dismutase/drug effects ; Tea
    Chemical Substances Capsules ; Drugs, Chinese Herbal ; Flavonoids ; Lipofuscin ; Phenols ; Polymers ; Polyphenols ; Tea ; lu-duo-wei ; Malondialdehyde (4Y8F71G49Q) ; Superoxide Dismutase (EC 1.15.1.1)
    Language English
    Publishing date 2000
    Publishing country Singapore
    Document type Journal Article
    ZDB-ID 193085-0
    ISSN 0192-415X ; 0090-2942
    ISSN 0192-415X ; 0090-2942
    DOI 10.1142/S0192415X00000301
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Effects of lu-duo-wei capsule on prolonging life span of housefly and Drosophila melanogaster.

    Cui, X / Dai, X G / Li, W B / Zhang, B L / Fang, Y Z

    The American journal of Chinese medicine

    1999  Volume 27, Issue 3-4, Page(s) 407–413

    Abstract: Lu-Duo-Wei capsule is a product of Chinese medicine having high efficacy in scavenging superoxide and hydroxyl radicals. It contains antioxidants, which may increase longevity, but whether Lu-Duo-Wei capsule has such an effect is unknown. In this study, ... ...

    Abstract Lu-Duo-Wei capsule is a product of Chinese medicine having high efficacy in scavenging superoxide and hydroxyl radicals. It contains antioxidants, which may increase longevity, but whether Lu-Duo-Wei capsule has such an effect is unknown. In this study, supplementing the basic diet with Lu-Duo-Wei resulted in prolonging the life span of houseflies and fruit flies. Moreover, the effect of prolonging the life span of houseflies by Lu-Duo-Wei was significantly higher than that of tea polyphenol. The result not only confirms our previous report but also supports the free radical theory of aging.
    MeSH term(s) Animals ; Drosophila melanogaster/physiology ; Drugs, Chinese Herbal/pharmacology ; Female ; Houseflies/physiology ; Life Expectancy ; Male ; Phenols/pharmacology ; Time Factors
    Chemical Substances Drugs, Chinese Herbal ; Phenols ; lu-duo-wei
    Language English
    Publishing date 1999
    Publishing country Singapore
    Document type Journal Article
    ZDB-ID 193085-0
    ISSN 0192-415X ; 0090-2942
    ISSN 0192-415X ; 0090-2942
    DOI 10.1142/S0192415X9900046X
    Database MEDical Literature Analysis and Retrieval System OnLINE

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