Article ; Online: Detection of exogenous DNA uptake by murine dendritic cells
STAR Protocols, Vol 3, Iss 3, Pp 101464- (2022)
2022
Abstract: Summary: This protocol has been developed to measure exogenous DNA uptake by murine dendritic cells (DCs) using supernatant containing cellular debris, which allows for DNA uptake in the absence of transfection reagents. Inhibitors or antibodies that ... ...
| Abstract | Summary: This protocol has been developed to measure exogenous DNA uptake by murine dendritic cells (DCs) using supernatant containing cellular debris, which allows for DNA uptake in the absence of transfection reagents. Inhibitors or antibodies that alter the process can be added, and either flow cytometry or fluorescent microscopy can be used to measure DNA uptake. This is intended to mimic the exposure of DCs to dying cells in the tumor microenvironment or other pathological conditions of high cellular death.For complete details on the use and execution of this protocol, please refer to de Mingo Pulido et al. (2021). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
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| Keywords | Cancer ; Cell Biology ; Cell isolation ; Flow Cytometry/Mass Cytometry ; Immunology ; Microscopy ; Science (General) ; Q1-390 |
| Language | English |
| Publishing date | 2022-09-01T00:00:00Z |
| Publisher | Elsevier |
| Document type | Article ; Online |
| Database | BASE - Bielefeld Academic Search Engine (life sciences selection) |
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