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  1. Article ; Online: Using Extract From the Stems and Leaves of Yizhi (Alpiniae oxyphyllae) as Feed Additive Increases Meat Quality and Intestinal Health in Ducks

    Fengjie Ji / Lihong Gu / Guang Rong / Chengjun Hu / Weiping Sun / Dingfa Wang / Weiqi Peng / Dajie Lin / Quanwei Liu / Hongzhi Wu / Haofu Dai / Hanlin Zhou / Tieshan Xu

    Frontiers in Veterinary Science, Vol

    2022  Volume 8

    Abstract: Yizhi (Alpiniae Oxyphyllae, A. oxyphylla) has been widely used as an important traditional Chinese medicinal herb for centuries. Existing studies have shown that A. oxyphylla has numerous benefits in human and animal health. We hypothesized that extract ... ...

    Abstract Yizhi (Alpiniae Oxyphyllae, A. oxyphylla) has been widely used as an important traditional Chinese medicinal herb for centuries. Existing studies have shown that A. oxyphylla has numerous benefits in human and animal health. We hypothesized that extract from the stems and leaves of A. oxyphylla (AOE) as a feed additive may have positive effects on animal health and products. Thus, this study was conducted to evaluate the effects of AOE as a feed additive on growth performance, serum biochemical parameters, intestinal morphology, microbial composition, and meat quality in Jiaji ducks. A total of 240 Jiaji ducks of 42 days old (1675.8 ± 44.2 g, male: female ratio = 1:1) were blocked based on body weight and randomly allocated into four dietary treatments with three replicates that each had 20 duck individuals. The dietary treatments included: basal diet, control group (CK); basal diet supplementation with 30 mg/kg (Y1), 80 mg/kg (Y2), and 130 mg/kg (Y3) AOE, respectively, and lasted for 49 days. The results showed that average daily feed intake from day 42 to day 60 was decreased with the increasing level of AOE (P < 0.05). Compared with the CK group, the groups with AOE supplementation decreased serum LDL-C level (P < 0.05), the addition of 30 mg/kg AOE increased total amino acids, essential amino acids, branched-chain amino acids, nonessential amino acids, and umami taste amino acids (P < 0.05), but decreased selenium and zinc concentrations in breast muscle (P < 0.05). In addition, the supplementation of 30 or 130 mg/kg AOE significantly increased jejunal villus height (P < 0.05) and tended to increase the ratio of villus height to crypt depth in the jejunum (P = 0.092) compared to the CK group. Moreover, the addition of 30 mg/kg AOE showed a higher abundance of genus unclassified Bacteroidales and genus unclassified Ruminococcaceae than the CK group (P < 0.05). Therefore, dietary supplementation with 30 mg/kg AOE increased meat nutrition profile and flavor through promoting amino acid ...
    Keywords Yizhi (Alpinia oxyphylla) ; ducks ; intestinal health ; antibiotic alternatives ; meat quality ; Veterinary medicine ; SF600-1100
    Subject code 630
    Language English
    Publishing date 2022-01-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Dietary Supplementation With Didancao (Elephantopus scaber L.) Improves Meat Quality and Intestinal Development in Jiaji Ducks

    Chengjun Hu / Lihong Gu / Mao Li / Fengjie Ji / Weiping Sun / Dingfa Wang / Weiqi Peng / Dajie Lin / Quanwei Liu / Haofu Dai / Hanlin Zhou / Tieshan Xu

    Frontiers in Veterinary Science, Vol

    2021  Volume 8

    Abstract: Didancao (Elephantopus scaber L.) has been used as a traditional herbal medicine and has exhibited a beneficial role in animal health. This study aimed to investigate the effects of dietary supplementation with E. scaber on growth performance, meat ... ...

    Abstract Didancao (Elephantopus scaber L.) has been used as a traditional herbal medicine and has exhibited a beneficial role in animal health. This study aimed to investigate the effects of dietary supplementation with E. scaber on growth performance, meat quality, intestinal morphology, and microbiota composition in ducks. A total of 480 Jiaji ducks (42 days old, male:female ratio = 1:1) were randomly assigned to one of four treatments. There were six replicates per treatment, with 20 ducks per replicate. The ducks in the control group (Con) were fed a basal diet; the three experimental groups were fed a basal diet supplementation with 30 (T1), 80 (T2), and 130 mg/kg (T3) of E. scaber. After a 48-day period of supplementation, growth performance, meat quality, intestinal morphology, and microbiota composition were evaluated. The results showed that no differences were observed in the final body weight, average daily feed intake, and average daily gain among the four groups. Compared with that in the Con group, the feed conversion in the T1 and T2 groups was increased significantly; the T2 group was shown to decrease the concentration of alanine aminotransferase in serum; the T3 group was lower than the Con group in the concentration of aspartate aminotransferase and was higher than the Con group in the concentration of high-density lipoprotein-cholesterol. The highest concentration of creatinine was observed in the T1 group. The T2 group was higher than the Con group in the contents of Phe, Ala, Gly, Glu, Arg, Lys, Tyr, Leu, Ser, Thr, Asp, and total amino acids in the breast muscle. Moreover, the T2 group was higher than the Con group in the contents of meat C18:2n−6 and polyunsaturated fatty acid. The concentration of inosinic acid in the T1, T2, and T3 groups was significantly higher than that in the Con group. However, the Con group was higher than the T2 or T3 group in the Zn content. The T2 group was lower than the Con group in the jejunal crypt depth. The T3 group was higher than the Con group in the ileal villus ...
    Keywords Elephantopus scaber L ; ducks ; feed conversion ratio ; growth performance ; meat quality ; Veterinary medicine ; SF600-1100
    Subject code 796 ; 630
    Language English
    Publishing date 2021-10-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Genetic characteristics of Jiaji Duck by whole genome re-sequencing.

    Lihong Gu / Feng Wang / Zhemin Lin / Tieshan Xu / Dajie Lin / Manping Xing / Shaoxiong Yang / Zhe Chao / Baoguo Ye / Peng Lin / Chunhui Hui / Lizhi Lu / Shuisheng Hou

    PLoS ONE, Vol 15, Iss 2, p e

    2020  Volume 0228964

    Abstract: Jiaji Duck (JJ) is a Muscovy duck species that possesses many superior characteristics, and it has become an important genetic resource in China. However, to date, its genetic characteristics and genetic relationship with other duck breeds have not been ... ...

    Abstract Jiaji Duck (JJ) is a Muscovy duck species that possesses many superior characteristics, and it has become an important genetic resource in China. However, to date, its genetic characteristics and genetic relationship with other duck breeds have not been explored yet, which greatly limits the utilization of JJ. In the present study, we investigated the genome sequences of 15 individual ducks representing five different duck populations, including JJ, French Muscovy duck (FF), mallard (YD), hong duck (HD) and Beijing duck (BD). Moreover, we investigated the characteristics of JJ-specific single nucleotide polymorphisms (SNPs) and compared the genome sequences of JJ vs. YD and JJ vs. BD using integrated strategies, including mutation detection, selective screening, and Gene Ontology (GO) analysis. More than 40 Gb of clean data were obtained for each population (mean coverage of 13.46 Gb per individual). A total number of 22,481,367 SNPs and 4,156,829 small insertion-deletions (Indels) were identified for the five duck populations, which could be used as molecular markers in breeding and utilization of JJ. Moreover, we identified 1,447,932 JJ-specific SNPs, and found that genes covering at least one JJ-specific SNP mainly involved in protein phosphorylation and dephosphorylation, as well as DNA modification. Phylogenetic tree and principal components analysis (PCA) revealed that the genetic relationship of JJ was closest to FF, while it was farthest to BD. A total of 120 and 111 genes were identified as positive selection genes for JJ vs. BD and JJ vs. YD, respectively. GO and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that the positive selection genes for JJ vs. BD ducks mainly involved in pigmentation, muscle contraction and stretch, gland secretion, and immunology, while the positive selection genes obtained from JJ vs. YD ducks mainly involved in embryo development, muscle contraction and stretch, and gland secretion. Taken together, our findings enabled us to better understand the ...
    Keywords Medicine ; R ; Science ; Q
    Subject code 590
    Language English
    Publishing date 2020-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Cellular delivery of quantum dot-bound hybridization probe for detection of intracellular pre-microRNA using chitosan/poly(γ-glutamic acid) complex as a carrier.

    Yao Geng / Dajie Lin / Lijia Shao / Feng Yan / Huangxian Ju

    PLoS ONE, Vol 8, Iss 6, p e

    2013  Volume 65540

    Abstract: A quantum dot (QD)-bound hybridization probe was designed for detection of intracellular pre-miRNA using chitosan (CS)/poly(γ-glutamic acid) (γ-PGA) complex as a gene vector. The probe was prepared by assembling thiolated RNA to gold nanoparticle (Au NP) ...

    Abstract A quantum dot (QD)-bound hybridization probe was designed for detection of intracellular pre-miRNA using chitosan (CS)/poly(γ-glutamic acid) (γ-PGA) complex as a gene vector. The probe was prepared by assembling thiolated RNA to gold nanoparticle (Au NP) via Au-S bond and then binding 3'-end amine of the RNA to the carboxy group capped on quantum dot surface. The QD-RNA-Au NP probe was assembled on the vector by mixing with aqueous γ-PGA solution and then CS solution to construct a gene delivery system for highly effective cellular uptake and delivery. After the probe was released from CS/γ-PGA complex to the cytoplasm by electrostatic repulsion at intracellular pH, it hybridized with pre-miRNA precursor as target. The formed product was then cleaved by RNase III Dicer, leading to the separation of QDs from Au NPs and fluorescence emission of QDs, which could be detected by confocal microscopic imaging to monitor the amount of the intracellular pre-miRNA precursor. The in vitro assays revealed that the QD-RNA-Au NP was a robust, sensitive and selective probe for quantitative detection of target pre-miRNA. Using MDA-MB231 and MCF-7 breast cancer cells as models, the relative amount of pre-miRNA let-7a could be successfully compared. Since the amount of miRNA is related to the progress and prognosis of cancer, this strategy could be expected to hold promising application potential in medical research and clinical diagnostics.
    Keywords Medicine ; R ; Science ; Q
    Subject code 500
    Language English
    Publishing date 2013-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article: Highly sensitive and selective electrochemical detection of Hg2+ through surface-initiated enzymatic polymerization

    Mei, Chenyang / Aili Liu / Chengchao Fan / Dajie Lin / Jichang Wang / Shun Wang

    Biosensors & bioelectronics. 2016 June 15, v. 80

    2016  

    Abstract: A Hg2+ electrochemical biosensor is developed by integrating thymine–Hg2+–thymine (T–Hg2+–T) base pairs for the high selectivity with surface-initiated enzymatic polymerization (SIEP) for signal amplification. The fabrication begins with the covalent ... ...

    Abstract A Hg2+ electrochemical biosensor is developed by integrating thymine–Hg2+–thymine (T–Hg2+–T) base pairs for the high selectivity with surface-initiated enzymatic polymerization (SIEP) for signal amplification. The fabrication begins with the covalent conjugation of capture DNA probe labeled with thiol at its 3′terminal onto the gold electrode. The presence of Hg2+ leads to DNA hybridization, in which complementary DNA was captured onto the biosensor surface, which subsequently catalyzed the addition of deoxynucleotides (dNTP) containing biotinlated 2′-deoxyadenosine 5′-triphosphate (biotin-dATP) by terminal deoxynucleotidyl transferase (TdT). The binding between biotin and strepavidin leads to the attachment of a large number of strepavidin functionalized silver nanoparticles (strepavidin-AgNPs), which could generate electrochemical stripping signal of silver to monitor the concentration of Hg2+ in KCl solution. Through utilizing the T–Hg2+–T selectivity and SIEP amplification, this assay method can detect aqueous Hg2+ with a wide linear range from 0.05nM to 100nM and a detection limit of 0.024nM. The application of this sensor in the analysis of drinking water demonstrates that the proposed method works well for real samples.
    Keywords biosensors ; biotin ; complementary DNA ; detection limit ; drinking water ; electrochemistry ; electrodes ; gold ; mercury ; nanosilver ; nucleic acid hybridization ; polymerization ; potassium chloride ; silver ; thiols
    Language English
    Dates of publication 2016-0615
    Size p. 105-110.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 1011023-9
    ISSN 1873-4235 ; 0956-5663
    ISSN (online) 1873-4235
    ISSN 0956-5663
    DOI 10.1016/j.bios.2016.01.009
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    Zs.A 2275: Show issues Location:
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  6. Article: Water-soluble benzoselenadiazole-based conjugated polymer fluorescent sensor with high selectivity for ferric ions and mercury ions and possible applications as integrated molecular logic gates

    Lei, Yunxiang / Dajie Lin / Huayue Wu / Hui Li / Jinchang Ding / Jiuxi Chen / Miaochang Liu / Wenxia Gao / Xiaobo Huang

    Tetrahedron. 2015 May 27, v. 71

    2015  

    Abstract: A conjugated polymer P-1 based on benzoselenadiazole was synthesized by the polymerization of 1,4-bis[3′-(N,N-diethylamino)-1′-oxapropyl]-2,5-diiodobenzene (M-1) and 4,7-diethynylbenzoselenadiazole (M-2) via Pd-catalyzed Sonogashira–Hagihara ... ...

    Abstract A conjugated polymer P-1 based on benzoselenadiazole was synthesized by the polymerization of 1,4-bis[3′-(N,N-diethylamino)-1′-oxapropyl]-2,5-diiodobenzene (M-1) and 4,7-diethynylbenzoselenadiazole (M-2) via Pd-catalyzed Sonogashira–Hagihara coupling reaction. The water-soluble conjugated polyelectrolyte P-2 was obtained by the reaction of P-1 with ethyl bromide. The responsive optical properties of P-1 and P-2 on various metal ions were investigated by fluorescence spectroscopy in real-time detection. P-1 showed highly sensitive and selective detection of Fe3+ by ‘turn-on’ fluorescence enhancement in DMSO/H2O. P-2 exhibited outstanding sense of Hg2+ using BSe as receptor by ‘turn-off’ fluorescence quench type and Fe3+ using unionized triethylamino group as receptor by ‘turn-on’ fluorescence enhancement type in MeOH/H2O, respectively. Additionally, the addition of EDTA to the P-2–metal complexes could result in the fluorescence recovery of the P-2 solution. Fluorescence spectral responses as output signals and two independent molecular input signals based on P-2 could be used to demonstrate important logic operation like INHIBIT and IMPLICATION gates.
    Keywords chemical structure ; EDTA (chelating agent) ; electrolytes ; fluorescence ; fluorescence emission spectroscopy ; ions ; iron ; mercury ; metal ions ; optical properties ; polymerization ; polymers
    Language English
    Dates of publication 2015-0527
    Size p. 3453-3462.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 204285-x
    ISSN 1464-5416 ; 0040-4020 ; 0563-2064
    ISSN (online) 1464-5416
    ISSN 0040-4020 ; 0563-2064
    DOI 10.1016/j.tet.2015.03.068
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    Zs.B 1076: Show issues Location:
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