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Article ; Online: Metal Imbalance in Neurodegenerative Diseases with a Specific Concern to the Brain of Multiple Sclerosis Patients.

Dales, Jean-Philippe / Desplat-Jégo, Sophie

International journal of molecular sciences

2020 Volume 21, Issue 23

Abstract: There is increasing evidence that deregulation of metals contributes to a vast range of neurodegenerative diseases including multiple sclerosis (MS). MS is a chronic inflammatory disease of the central nervous system (CNS) manifesting disability and ... ...

Abstract	There is increasing evidence that deregulation of metals contributes to a vast range of neurodegenerative diseases including multiple sclerosis (MS). MS is a chronic inflammatory disease of the central nervous system (CNS) manifesting disability and neurological symptoms. The precise origin of MS is unknown, but the disease is characterized by focal inflammatory lesions in the CNS associated with an autoimmune reaction against myelin. The treatment of this disease has mainly been based on the prescription of immunosuppressive and immune-modulating agents. However, the rate of progressive disability and early mortality is still worrisome. Metals may represent new diagnostic and predictive markers of severity and disability as well as innovative candidate drug targets for future therapies. In this review, we describe the recent advances in our understanding on the role of metals in brain disorders of neurodegenerative diseases and MS patients.
MeSH term(s)	Animals ; Brain/pathology ; Homeostasis ; Humans ; Inflammation/pathology ; Metals/metabolism ; Multiple Sclerosis/metabolism ; Neurodegenerative Diseases/metabolism
Chemical Substances	Metals
Language	English
Publishing date	2020-11-30
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms21239105
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Article ; Online: Transformation from lung adenocarcinoma to combined small cell carcinoma in pleural effusion after treatment with osimertinib.

Frankel, Diane / Kaspi, Elise / Liprandi, Agnès / Dales, Jean-Philippe / Roll, Patrice

Cytopathology : official journal of the British Society for Clinical Cytology

2022 Volume 33, Issue 5, Page(s) 633–635

MeSH term(s)	Acrylamides ; Adenocarcinoma of Lung/drug therapy ; Aniline Compounds ; Carcinoma, Small Cell ; Humans ; Indoles ; Lung Neoplasms/drug therapy ; Lung Neoplasms/pathology ; Pleural Effusion ; Pleural Effusion, Malignant/drug therapy ; Pleural Effusion, Malignant/pathology ; Pyrimidines
Chemical Substances	Acrylamides ; Aniline Compounds ; Indoles ; Pyrimidines ; osimertinib (3C06JJ0Z2O)
Language	English
Publishing date	2022-06-24
Publishing country	England
Document type	Case Reports
ZDB-ID	1034190-0
ISSN	1365-2303 ; 0956-5507 ; 1350-4037
ISSN (online)	1365-2303
ISSN	0956-5507 ; 1350-4037
DOI	10.1111/cyt.13156
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Article ; Online: TWEAK and TNFα, Both TNF Ligand Family Members and Multiple Sclerosis-Related Cytokines, Induce Distinct Gene Response in Human Brain Microvascular Endothelial Cells.

Stephan, Delphine / Roger, Anais / Aghzadi, Jehanne / Carmona, Sylvie / Picard, Christophe / Dales, Jean-Philippe / Desplat-Jégo, Sophie

Genes

2022 Volume 13, Issue 10

Abstract: Tumor necrosis factor-like weak inducer of apoptosis (TWEAK) is a member of the TNF ligand family involved in various diseases including brain inflammatory pathologies such as multiple sclerosis. It has been demonstrated that TWEAK can induce ... ...

Abstract	Tumor necrosis factor-like weak inducer of apoptosis (TWEAK) is a member of the TNF ligand family involved in various diseases including brain inflammatory pathologies such as multiple sclerosis. It has been demonstrated that TWEAK can induce cerebrovascular permeability in an in vitro model of the blood-brain barrier. The molecular mechanisms playing a role in TWEAK versus TNFα signaling on cerebral microvascular endothelial cells are not well defined. Therefore, we aimed to identify gene expression changes in cultures of human brain microvascular endothelial cells (hCMEC/D3) to address changes initiated by TWEAK exposure. Taken together, our studies highlighted that gene involved in leukocyte extravasation, notably claudin-5, were differentially modulated by TWEAK and TNFα. We identified differential gene expression of hCMEC/D3 cells at three timepoints following TWEAK versus TNFα stimulation and also found distinct modulations of several canonical pathways including the actin cytoskeleton, vascular endothelial growth factor (VEGF), Rho family GTPases, and phosphatase and tensin homolog (PTEN) pathways. To our knowledge, this is the first study to interrogate and compare the effects of TWEAK versus TNFα on gene expression in brain microvascular endothelial cells.
MeSH term(s)	Humans ; Brain ; Claudin-5 ; Cytokines ; Endothelial Cells ; Family ; GTP Phosphohydrolases ; Ligands ; Multiple Sclerosis/genetics ; Phosphoric Monoester Hydrolases ; Tensins ; Tumor Necrosis Factor-alpha/genetics ; Tumor Necrosis Factor-alpha/pharmacology ; Vascular Endothelial Growth Factor A ; Vascular Endothelial Growth Factors
Chemical Substances	Claudin-5 ; Cytokines ; GTP Phosphohydrolases (EC 3.6.1.-) ; Ligands ; Phosphoric Monoester Hydrolases (EC 3.1.3.2) ; Tensins ; Tumor Necrosis Factor-alpha ; Vascular Endothelial Growth Factor A ; Vascular Endothelial Growth Factors ; TNFSF12 protein, human
Language	English
Publishing date	2022-09-24
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2527218-4
ISSN	2073-4425 ; 2073-4425
ISSN (online)	2073-4425
ISSN	2073-4425
DOI	10.3390/genes13101714
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Article ; Online: Hypoxia-inducible factor-1α as prognostic marker.

Ben Lassoued, Amin / Beaufils, Nathalie / Dales, Jean-Philippe / Gabert, Jean

Expert opinion on medical diagnostics

2013 Volume 7, Issue 1, Page(s) 53–70

Abstract: Introduction: Hypoxia-inducible factor-1α (HIF-1α) is a key player in the signaling pathway that mediates a complex and pleiotropic range of adaptive responses to hypoxia. It serves as cellular hypoxia sensor and plays a critical role in physiologic ... ...

Abstract	Introduction: Hypoxia-inducible factor-1α (HIF-1α) is a key player in the signaling pathway that mediates a complex and pleiotropic range of adaptive responses to hypoxia. It serves as cellular hypoxia sensor and plays a critical role in physiologic processes including glucose metabolism, iron metabolism, erythropoiesis, angiogenesis, cell survival and apoptosis, but also, pathologic processes such as carcinogenesis, progression and metastasis of many cancers. With the recent advent of new molecular targeted therapies, there is a growing need of molecular understanding of physiology and physiopathology and increased demand of diagnosis, prognosis and follow-up markers. Areas covered: This paper reviews the biology of regulation of HIF-1α, its physiological and physiopathological effects. Expert opinion: The authors discuss the potential diagnosis and the prognosis significance of HIF-1α that was evaluated in recent studies.
MeSH term(s)	Biomarkers/analysis ; Heart Diseases/diagnosis ; Humans ; Hypoxia-Inducible Factor 1, alpha Subunit/analysis ; Inflammation/diagnosis ; Neoplasms/chemistry ; Neoplasms/diagnosis ; Prognosis
Chemical Substances	Biomarkers ; Hypoxia-Inducible Factor 1, alpha Subunit
Language	English
Publishing date	2013-01
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	2393880-8
ISSN	1753-0067 ; 1753-0059
ISSN (online)	1753-0067
ISSN	1753-0059
DOI	10.1517/17530059.2012.719022
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Article ; Online: Positive FDG-PET/CT of the pleura twenty years after talc pleurodesis: three cases of benign talcoma.

Vandemoortele, Thomas / Laroumagne, Sophie / Roca, Elisa / Bylicki, Olivier / Dales, Jean-Philippe / Dutau, Hervé / Astoul, Philippe

Respiration; international review of thoracic diseases

2014 Volume 87, Issue 3, Page(s) 243–248

Abstract: The FDG-PET (fluorine-18 fluorodeoxyglucose positron emission tomography) scan is used with increasing frequency to investigate pleural abnormalities and to determine the possibility of neoplastic invasion. However, false-positive findings are not ... ...

Abstract	The FDG-PET (fluorine-18 fluorodeoxyglucose positron emission tomography) scan is used with increasing frequency to investigate pleural abnormalities and to determine the possibility of neoplastic invasion. However, false-positive findings are not uncommon and talc pleurodesis has been reported to cause hypermetabolic pleural thickenings up to 5 years after the procedure. We report the cases of 3 patients (2 of whom had a history of asbestos exposure) requiring talc pleurodesis for recurrent pneumothoraces between 1988 and 1990, who were investigated in 2011 for pleural abnormalities. Avid pleural thickening on FDG-PET scan mimicking pleural cancer was found, but this was deemed secondary to the pleurodesis. Talc pleurodesis generates inflammation which promotes pleural adhesions. This inflammatory reaction could decrease with time, as in other inflammatory processes. Since talc is not metabolized by the body, the FDG-PET scan can remain positive, most likely because of a foreign-body granulomatous reaction, even 20 years later. It is important to be aware of this possibility and to question patients with pleural abnormalities about past procedures and mention such procedures to the colleagues who are responsible for interpreting metabolic imaging. Follow-up of hypermetabolic pleural lesions attributed to talc pleurodesis is important for the detection of new pleural lesions or neoplastic evolution.
MeSH term(s)	Adult ; Diagnostic Errors ; False Positive Reactions ; Fluorodeoxyglucose F18 ; Granuloma, Foreign-Body/diagnostic imaging ; Granuloma, Foreign-Body/etiology ; Humans ; Male ; Middle Aged ; Multimodal Imaging ; Pleural Diseases/diagnostic imaging ; Pleural Diseases/etiology ; Pleural Neoplasms/diagnostic imaging ; Pleurodesis ; Positron-Emission Tomography ; Radiopharmaceuticals ; Talc/adverse effects ; Tomography, X-Ray Computed
Chemical Substances	Radiopharmaceuticals ; Fluorodeoxyglucose F18 (0Z5B2CJX4D) ; Talc (14807-96-6)
Language	English
Publishing date	2014
Publishing country	Switzerland
Document type	Case Reports ; Journal Article
ZDB-ID	206674-9
ISSN	1423-0356 ; 0025-7931
ISSN (online)	1423-0356
ISSN	0025-7931
DOI	10.1159/000356752
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Article ; Online: Subcutaneous infection with Dirofilaria immitis nematode in human, France.

Foissac, Maud / Million, Matthieu / Mary, Charles / Dales, Jean-Philippe / Souraud, Jean-Baptiste / Piarroux, Renaud / Parola, Philippe

Emerging infectious diseases

2012 Volume 19, Issue 1, Page(s) 171–172

MeSH term(s)	Animals ; Dirofilaria immitis/genetics ; Dirofilaria immitis/isolation & purification ; Dirofilariasis/diagnosis ; Dirofilariasis/parasitology ; Female ; France ; Humans ; Middle Aged ; Polymerase Chain Reaction ; RNA, Ribosomal/genetics
Chemical Substances	RNA, Ribosomal ; RNA, ribosomal, 12S
Language	English
Publishing date	2012-12-18
Publishing country	United States
Document type	Case Reports ; Letter
ZDB-ID	1380686-5
ISSN	1080-6059 ; 1080-6040
ISSN (online)	1080-6059
ISSN	1080-6040
DOI	10.3201/eid1901.120281
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Zs.A 4778: Show issues

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Article ; Online: Analysis of T-cell receptor-γ gene rearrangements using heteroduplex analysis by high-resolution microcapillary electrophoresis.

Beaufils, Nathalie / Ben Lassoued, Amin / Essaydi, Arnaud / Dales, Jean-Philippe / Formisano-Tréziny, Christine / Bonnet, Nathalie / Grob, Jean-Jacques / Gabert, Jean

Leukemia research

2012 Volume 36, Issue 9, Page(s) 1119–1123

Abstract: Determination of T-cell clonality has an important additional value for diagnosis of T-cell lymphomas. Various molecular methods have been developed, including polymerase chain reaction (PCR) of T-cell receptor γ (TCRγ). The detection of PCR products ... ...

Abstract	Determination of T-cell clonality has an important additional value for diagnosis of T-cell lymphomas. Various molecular methods have been developed, including polymerase chain reaction (PCR) of T-cell receptor γ (TCRγ). The detection of PCR products usually relies commonly on either GeneScan (GS) analysis or heteroduplex (HD) analysis by polyacrylamide gel electrophoresis (PAGE). These techniques have their disadvantages, being relatively time-consuming and laborious or requiring expensive equipment. Here, we propose an alternative method that combines multiplex PCR and HD analysis by microcapillary electrophoresis (ME) on the Agilent 2100 Bioanalyzer. The sensitivity of the method was determined with clonal PEER T-cell line DNA dilution in polyclonal DNA and was evaluated as 1-5%. Fifty-three samples from patients with T-cell lymphoproliferative disorders were analyzed by HD analysis using ME and GS analyses. Comparison of the two techniques showed them to be highly concordant (93% similarity). The rate of clonality detection by GS analysis was higher than HD analysis by ME, but none of the discordant patients (n=5) has yet developed lymphoma. HD analysis by ME to reveal TCRγ gene rearrangements in clinical specimens was consistent with clinical data and the outcome of patients. Detection of T-cell clonality by HD analysis with ME is sensitive, practical, safe and represents a potential alternative to PAGE and GS analysis.
MeSH term(s)	Clone Cells/metabolism ; Efficiency ; Electrophoresis, Capillary/methods ; Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor ; Genes, T-Cell Receptor gamma/genetics ; Heteroduplex Analysis/methods ; Humans ; Lymphoproliferative Disorders/diagnosis ; Lymphoproliferative Disorders/genetics ; Microchemistry/methods ; Retrospective Studies ; Sensitivity and Specificity
Language	English
Publishing date	2012-09
Publishing country	England
Document type	Evaluation Studies ; Journal Article ; Validation Studies
ZDB-ID	752396-8
ISSN	1873-5835 ; 0145-2126
ISSN (online)	1873-5835
ISSN	0145-2126
DOI	10.1016/j.leukres.2012.06.003
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Article: Positive FDG-PET/CT der Pleura 20 Jahre nach Talkumpleurodese: Drei Fälle von benignem Talkom

Vandemoortele, Thomas / Laroumagne, Sophie / Roca, Elisa / Bylicki, Olivier / Dales, Jean-Philippe / Dutau, Hervé / Astoul, Philippe

Karger Kompass Pneumologie

2014 Volume 2, Issue 2, Page(s) 105–110

Abstract: Immer häufiger wird der Positronen-Emissions-Tomographie-Scan mit 18F-Fluordesoxyglucose (FDG-PET-Scan) genutzt, um Anomalien der Pleura zu untersuchen und die Möglichkeit eines neoplastischen Befalls zu prüfen. Falsch-positive Befunde sind jedoch nicht ... ...

Institution	Department of Thoracic Oncology, Pleural Diseases and Interventional Pulmonology, Hôpital Nord Aix-Marseille University Department of Pathology, Hôpital de la Timone, Marseille, France Department of Pulmonology, Centre hospitalier de l'Université de Montréal, Hôpital Notre-Dame, Montréal, QC, Canada
Abstract	Immer häufiger wird der Positronen-Emissions-Tomographie-Scan mit 18F-Fluordesoxyglucose (FDG-PET-Scan) genutzt, um Anomalien der Pleura zu untersuchen und die Möglichkeit eines neoplastischen Befalls zu prüfen. Falsch-positive Befunde sind jedoch nicht selten, und die Talkumpleurodese verursacht Berichten zufolge noch bis zu 5 Jahre nach der Durchführung hypermetabolische Pleuraverdickungen. Wir berichten über die Fälle von 3 Patienten (2 davon mit einer Vorgeschichte von Asbestexposition), die zwischen 1988 und 1990 wegen wiederkehrendem Pneumothorax mit einer Talkumpleurodese behandelt und 2011 erneut auf pleurale Veränderungen untersucht wurden. Zwar wurden ausgeprägte Pleuraverdickungen, die dem Bild eines Pleuratumors ähnelten, im FDG-PET-Scan gefunden, jedoch als Folge der Pleurodese eingestuft. Die Talkumpleurodese löst eine entzündliche Reaktion aus, die pleurale Adhäsionen fördert. Diese Entzündungsreaktion könnte mit der Zeit schwächer werden, wie es auch bei anderen entzündlichen Prozessen der Fall ist. Da Talkum vom Körper nicht abgebaut wird, kann der FDG-PET-Scan jedoch auch 20 Jahre später noch positiv sein - vermutlich infolge einer granulomatösen Fremdkörperreaktion. Es ist wichtig, sich dieser Möglichkeit bewusst zu sein und Patienten mit pleuralen Veränderungen zu früheren Eingriffen zu befragen bzw. Kollegen, die für die Interpretation der stoffwechselbezogenen bildgebenden Untersuchungen zuständig sind, über solche Eingriffe zu informieren. Die Nachbeobachtung von hypermetabolischen pleuralen Läsionen, die einer Talkumpleurodese zugeschrieben werden, ist wichtig, um neue pleurale Veränderungen oder neoplastische Entwicklungen zu erkennen.
Keywords	FDG-PET/CT ; Talkumpleurodese ; Thorakoskopie ; Pneumothorax ; Pleurale Entzündungsreaktion
Language	German
Publishing date	2014-08-30
Publisher	S. Karger GmbH
Publishing place	Freiburg, Germany
Document type	Article
Note	Erfahrung aus der Praxis
ZDB-ID	2724124-5
ISSN	2296-0317 ; 2296-0368
ISSN (online)	2296-0317
ISSN	2296-0368
DOI	10.1159/000363476
Database	Karger publisher's database

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Article: Positive FDG-PET/CT of the Pleura Twenty Years after Talc Pleurodesis: Three Cases of Benign Talcoma

Vandemoortele, Thomas / Laroumagne, Sophie / Roca, Elisa / Bylicki, Olivier / Dales, Jean-Philippe / Dutau, Hervé / Astoul, Philippe

Respiration

2014 Volume 87, Issue 3, Page(s) 243–248

Institution	Department of Thoracic Oncology, Pleural Diseases and Interventional Pulmonology, Hôpital Nord Aix-Marseille University and Department of Pathology, Hôpital de la Timone, Marseille, France Department of Pulmonology, Centre hospitalier de l'Université de Montréal, Hôpital Notre-Dame, Montréal, Qué., Canada
Abstract	The FDG-PET (fluorine-18 fluorodeoxyglucose positron emission tomography) scan is used with increasing frequency to investigate pleural abnormalities and to determine the possibility of neoplastic invasion. However, false-positive findings are not uncommon and talc pleurodesis has been reported to cause hypermetabolic pleural thickenings up to 5 years after the procedure. We report the cases of 3 patients (2 of whom had a history of asbestos exposure) requiring talc pleurodesis for recurrent pneumothoraces between 1988 and 1990, who were investigated in 2011 for pleural abnormalities. Avid pleural thickening on FDG-PET scan mimicking pleural cancer was found, but this was deemed secondary to the pleurodesis. Talc pleurodesis generates inflammation which promotes pleural adhesions. This inflammatory reaction could decrease with time, as in other inflammatory processes. Since talc is not metabolized by the body, the FDG-PET scan can remain positive, most likely because of a foreign-body granulomatous reaction, even 20 years later. It is important to be aware of this possibility and to question patients with pleural abnormalities about past procedures and mention such procedures to the colleagues who are responsible for interpreting metabolic imaging. Follow-up of hypermetabolic pleural lesions attributed to talc pleurodesis is important for the detection of new pleural lesions or neoplastic evolution.
Keywords	Pleural inflammation ; FDG-PET/CT ; Talc pleurodesis ; Thoracoscopy ; Pneumothorax
Language	English
Publishing date	2014-01-18
Publisher	S. Karger AG
Publishing place	Basel, Switzerland
Document type	Article
Note	Novel Insights from Clinical Practice
ZDB-ID	206674-9
ISSN	1423-0356 ; 0025-7931
ISSN (online)	1423-0356
ISSN	0025-7931
DOI	10.1159/000356752
Database	Karger publisher's database

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Article ; Online: Hypoxia inducible factor 1α gene ( HIF-1α ) splice variants

Bonnier Pascal / Formisano-Tréziny Christine / Pradel Vincent / Pauly Vanessa / Picard Christophe / Silvy Monique / Beaufils Nathalie / Dales Jean-Philippe / Giusiano Sophie / Charpin Colette / Gabert Jean

BMC Medicine, Vol 8, Iss 1, p

potential prognostic biomarkers in breast cancer

2010 Volume 44

Abstract: Abstract Background Hypoxia-inducible factor 1 (HIF-1) is a master transcriptional regulator of genes regulating oxygen homeostasis. The HIF-1 protein is composed of two HIF-1α and HIF-1β/aryl hydrocarbon receptor nuclear translocator (ARNT) subunits. ... ...

Abstract	Abstract Background Hypoxia-inducible factor 1 (HIF-1) is a master transcriptional regulator of genes regulating oxygen homeostasis. The HIF-1 protein is composed of two HIF-1α and HIF-1β/aryl hydrocarbon receptor nuclear translocator (ARNT) subunits. The prognostic relevance of HIF-1α protein overexpression has been shown in breast cancer. The impact of HIF-1α alternative splice variant expression on breast cancer prognosis in terms of metastasis risk is not well known. Methods Using real-time quantitative reverse transcription PCR assays, we measured mRNA concentrations of total HIF-1α and 4 variants in breast tissue specimens in a series of 29 normal tissues or benign lesions (normal/benign) and 53 primary carcinomas. In breast cancers HIF-1α splice variant levels were compared to clinicopathological parameters including tumour microvessel density and metastasis-free survival. Results HIF-1α isoforms containing a three base pairs TAG insertion between exon 1 and exon 2 (designated HIF-1α TAG ) and HIF-1α 736 mRNAs were found expressed at higher levels in oestrogen receptor (OR)-negative carcinomas compared to normal/benign tissues ( P = 0.009 and P = 0.004 respectively). In breast carcinoma specimens, lymph node status was significantly associated with HIF-1α TAG mRNA levels ( P = 0.037). Significant statistical association was found between tumour grade and HIF-1α TAG ( P = 0.048), and total HIF-1α ( P = 0.048) mRNA levels. HIF-1α TAG mRNA levels were also inversely correlated with both oestrogen and progesterone receptor status ( P = 0.005 and P = 0.033 respectively). Univariate analysis showed that high HIF-1α TAG mRNA levels correlated with shortened metastasis free survival ( P = 0.01). Conclusions Our results show for the first time that mRNA expression of a HIF-1α TAG splice variant reflects a stage of breast cancer progression and is associated with a worse prognosis. See commentary: http://www.biomedcentral.com/1741-7015/8/45
Keywords	Medicine ; R
Language	English
Publishing date	2010-07-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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