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Article ; Online: Papel del Taller de Lengua en la Facultad de Educación

Darío Elías Quiróz

Lúdica Pedagógica, Vol 1, Iss

2015 Volume 7

Abstract: Con el deseo fervoroso de aportar al desarrollo del lema del congreso (Educación Física, conocimiento y construcción), someto a discusión algunas reflexiones sobre el Papel del taller de lengua en la Facultad de Educación Física. He tratado de ordenar mi ...

Abstract	Con el deseo fervoroso de aportar al desarrollo del lema del congreso (Educación Física, conocimiento y construcción), someto a discusión algunas reflexiones sobre el Papel del taller de lengua en la Facultad de Educación Física. He tratado de ordenar mi ensayo así: primero, la introducción que resume el mismo, ubica el por qué de él y sus objetivos; segundo, una síntesis de las concepciones básicas de las cuales partimos; tercero, presentación de algunas creaciones estudiantiles que posibilitó nuestro taller; finalmente, algunas conclusiones sin el criterio de agotar el tema.
Keywords	Geography. Anthropology. Recreation ; G ; Recreation. Leisure ; GV1-1860 ; Sports ; GV557-1198.995
Language	Spanish
Publishing date	2015-06-01T00:00:00Z
Publisher	Universidad Pedagógica Nacional
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Carbon fixation and rhodopsin systems in microbial mats from hypersaline lakes Brava and Tebenquiche, Salar de Atacama, Chile.

Daniel Kurth / Dario Elias / María Cecilia Rasuk / Manuel Contreras / María Eugenia Farías

PLoS ONE, Vol 16, Iss 2, p e

2021 Volume 0246656

Abstract: In this work, molecular diversity of two hypersaline microbial mats was compared by Whole Genome Shotgun (WGS) sequencing of environmental DNA from the mats. Brava and Tebenquiche are lakes in the Salar de Atacama, Chile, where microbial communities are ... ...

Abstract	In this work, molecular diversity of two hypersaline microbial mats was compared by Whole Genome Shotgun (WGS) sequencing of environmental DNA from the mats. Brava and Tebenquiche are lakes in the Salar de Atacama, Chile, where microbial communities are growing in extreme conditions, including high salinity, high solar irradiance, and high levels of toxic metals and metaloids. Evaporation creates hypersaline conditions in these lakes and mineral precipitation is a characteristic geomicrobiological feature of these benthic ecosystems. The mat from Brava was more rich and diverse, with a higher number of different taxa and with species more evenly distributed. At the phylum level, Proteobacteria, Cyanobacteria, Chloroflexi, Bacteroidetes and Firmicutes were the most abundant, including ~75% of total sequences. At the genus level, the most abundant sequences were affilitated to anoxygenic phototropic and cyanobacterial genera. In Tebenquiche mats, Proteobacteria and Bacteroidetes covered ~70% of the sequences, and 13% of the sequences were affiliated to Salinibacter genus, thus addressing the lower diversity. Regardless of the differences at the taxonomic level, functionally the two mats were similar. Thus, similar roles could be fulfilled by different organisms. Carbon fixation through the Wood-Ljungdahl pathway was well represented in these datasets, and also in other mats from Andean lakes. In spite of presenting less taxonomic diversity, Tebenquiche mats showed increased abundance and variety of rhodopsin genes. Comparison with other metagenomes allowed identifying xantorhodopsins as hallmark genes not only from Brava and Tebenquiche mats, but also for other mats developing at high altitudes in similar environmental conditions.
Keywords	Medicine ; R ; Science ; Q
Subject code	550
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Diseases during pregnancy in a large unselected South American sample

María Rita Santos / Hebe Campaña / Silvina Heisecke / Julia Ratowiecki / Darío Elías / Lucas Giménez / Fernando Adrián Poletta / Juan Gili / Rocío Uranga / Viviana Cosentino / Hugo Krupitzki / Mónica Rittler / Jorge López Camelo

Revista Brasileira de Epidemiologia, Vol

2022 Volume 25

Abstract: ABSTRACT Objective: Our aim was to describe the prevalence of diseases during pregnancy and the association between fetal exposure to the most frequent maternal diseases and the risk of preterm (PTB) and/or small for gestational age (SGA) newborns in an ... ...

Abstract	ABSTRACT Objective: Our aim was to describe the prevalence of diseases during pregnancy and the association between fetal exposure to the most frequent maternal diseases and the risk of preterm (PTB) and/or small for gestational age (SGA) newborns in an unselected sample of women who gave birth in South American countries. Methods: We conducted a descriptive, cross-sectional study including 56,232 mothers of non-malformed infants born between 2002 and 2016, using data from the Latin American Collaborative Study of Congenital Malformations (ECLAMC). Diseases with higher- than-expected PTB/SGA frequencies were identified. Odds ratios of confounding variables for diseases and birth outcomes were calculated with a multivariable logistic regression. Results: Of the 14 most reported diseases, hypertension, genitourinary infection, epilepsy, hypothyroidism, diabetes, and HIV/AIDS showed higher PTB and/or SGA frequencies. Advanced and low maternal age, previous fetal loss, low socioeconomic level, and African-American ancestry were associated with PTB, while advanced maternal age, primigravidity, previous fetal loss, low socioeconomic level, and African-American ancestry were associated with SGA. After adjusting for the associated variables, the identified illnesses maintained their association with PTB and all, except epilepsy, with SGA. Conclusion: The description of an unselected population of mothers allowed identifying the most frequent diseases occurring during gestation and their impact on pregnancy outcomes. Six diseases were associated with PTB and two with SGA newborns. To the best of our knowledge, there are no similar reports about women not intentionally selected by specific diseases during pregnancy in South American populations.
Keywords	Pregnancy ; Disease ; Pregnancy complications ; infectious ; Chronic disease ; Infant ; small for gestational age ; premature ; Public aspects of medicine ; RA1-1270
Subject code	610
Language	English
Publishing date	2022-12-01T00:00:00Z
Publisher	Associação Brasileira de Pós-Graduação em Saúde Coletiva
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Combination therapy using nitro compounds improves the efficacy of experimental Chagas disease treatment.

Mazzeti, Ana Lia / Gonçalves, Karolina R / Mota, Suianne L A / Pereira, Dário Elias / Diniz, Lívia de F / Bahia, Maria Terezinha

Parasitology

2021 Volume 148, Issue 11, Page(s) 1320–1327

Abstract: Drug combinations have been evaluated for Chagas disease in an attempt to improve efficacy and safety. In this line, the objective of this work is to assess the effects of treatment with nitro drugs combinations using benznidazole (BZ) or nifurtimox (NFX) ...

Abstract	Drug combinations have been evaluated for Chagas disease in an attempt to improve efficacy and safety. In this line, the objective of this work is to assess the effects of treatment with nitro drugs combinations using benznidazole (BZ) or nifurtimox (NFX) plus the sulfone metabolite of fexinidazole (fex-SFN) in vitro and in vivo on Trypanosoma cruzi infection. The in vitro interaction of fex-SFN and BZ or NFX against infected H9c2 cells by the Y strain was classified as an additive (0.5⩾ΣFIC<4), suggesting the possibility of a dose reduction in the in vivo T. cruzi infection. Next, the effect of combining suboptimal doses was assessed in an acute model of murine T. cruzi infection. Drug combinations led to a faster suppression of parasitemia than monotherapies. Also, the associations led to higher cure levels than those in the reference treatment BZ 100 mg day−1 (57.1%) (i.e. 83.3% with BZ/fex-SFN and 75% with NFX/fex-SFN). Importantly, toxic effects resulting from the associations were not observed, according to weight gain and hepatic enzyme levels in the serum of experimental animals. Taken together, this study is a starting point to explore the potential effects of nitro drugs combinations in preclinical models of kinetoplastid-related infections.
MeSH term(s)	Animals ; Chagas Disease/drug therapy ; Drug Therapy, Combination ; Female ; Humans ; Inhibitory Concentration 50 ; Mice ; Neglected Diseases/drug therapy ; Nifurtimox/adverse effects ; Nifurtimox/therapeutic use ; Nitro Compounds/adverse effects ; Nitro Compounds/therapeutic use ; Nitroimidazoles/adverse effects ; Nitroimidazoles/metabolism ; Nitroimidazoles/therapeutic use ; Real-Time Polymerase Chain Reaction ; Sulfones/adverse effects ; Sulfones/therapeutic use
Chemical Substances	Nitro Compounds ; Nitroimidazoles ; Sulfones ; fexinidazole (306ERL82IR) ; Nifurtimox (M84I3K7C2O) ; benzonidazole (YC42NRJ1ZD)
Language	English
Publishing date	2021-06-18
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	207627-5
ISSN	1469-8161 ; 0031-1820
ISSN (online)	1469-8161
ISSN	0031-1820
DOI	10.1017/S0031182021001001
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Article: Combination therapy using nitro compounds improves the efficacy of experimental Chagas disease treatment

Mazzeti, Ana Lia / Gonçalves, Karolina R. / Mota, Suianne L. A. / Pereira, Dário Elias / Diniz, Lívia de F. / Bahia, Maria Terezinha

Parasitology. 2021 Sept., v. 148, no. 11

2021

Abstract	Drug combinations have been evaluated for Chagas disease in an attempt to improve efficacy and safety. In this line, the objective of this work is to assess the effects of treatment with nitro drugs combinations using benznidazole (BZ) or nifurtimox (NFX) plus the sulfone metabolite of fexinidazole (fex-SFN) in vitro and in vivo on Trypanosoma cruzi infection. The in vitro interaction of fex-SFN and BZ or NFX against infected H9c2 cells by the Y strain was classified as an additive (0.5⩾ΣFIC<4), suggesting the possibility of a dose reduction in the in vivo T. cruzi infection. Next, the effect of combining suboptimal doses was assessed in an acute model of murine T. cruzi infection. Drug combinations led to a faster suppression of parasitemia than monotherapies. Also, the associations led to higher cure levels than those in the reference treatment BZ 100 mg day⁻¹ (57.1%) (i.e. 83.3% with BZ/fex-SFN and 75% with NFX/fex-SFN). Importantly, toxic effects resulting from the associations were not observed, according to weight gain and hepatic enzyme levels in the serum of experimental animals. Taken together, this study is a starting point to explore the potential effects of nitro drugs combinations in preclinical models of kinetoplastid-related infections.
Keywords	Chagas disease ; Trypanosoma cruzi ; benznidazole ; blood serum ; enzymes ; metabolites ; mice ; models ; parasitemia ; parasitology ; toxicity ; weight gain
Language	English
Dates of publication	2021-09
Size	p. 1320-1327.
Publishing place	Cambridge University Press
Document type	Article
ZDB-ID	207627-5
ISSN	1469-8161 ; 0031-1820
ISSN (online)	1469-8161
ISSN	0031-1820
DOI	10.1017/S0031182021001001
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Inequidades sociales en madres adolescentes y la relación con resultados perinatales adversos en poblaciones sudamericanas

Julia Ratowiecki / María Rita Santos / Fernando Poletta / Silvina Heisecke / Dario Elias / Juan Gili / Lucas Gimenez / Mariela Pawluk / Rocio Uranga / Viviana Cosentino / Hebe Campaña / Mónica Rittler / Jorge S. López Camelo

Cadernos de Saúde Pública, Vol 36, Iss

2021 Volume 12

Abstract: Resumen: El objetivo fue explicar las diferencias en la frecuencia de eventos perinatales adversos entre madres adolescentes con baja y alta escolaridad. La muestra poblacional se recogió en la base de datos del Estudio Colaborativo Latinoamericano de ... ...

Abstract	Resumen: El objetivo fue explicar las diferencias en la frecuencia de eventos perinatales adversos entre madres adolescentes con baja y alta escolaridad. La muestra poblacional se recogió en la base de datos del Estudio Colaborativo Latinoamericano de Malformaciones Congénitas (ECLAMC). Entre 2.443.747 nacimientos ocurridos en 93 hospitales, se reclutaron 66.755 recién nacidos vivos, sin defectos congénitos, durante el período 2000-2017. Las madres adolescentes se clasificaron según su escolaridad en: baja, media y alta. Se utilizó un modelo multivariado, que incluyó efectos reproductivos, acceso a servicios de salud, variables demográficas-socioeconómicas, así como de grupo étnico. El modelo de descomposición de Fairlie se aplicó para cuantificar la contribución de variables explicativas en las frecuencias de eventos perinatales adversos. De los 66.755 recién nacidos investigados, el 21,1% (n = 14.078) fue primigrávida de madres adolescentes. La distribución por escolaridad materna fue de 24,2%, 59,8% y 16% para baja, media y alta escolaridad, respectivamente. Las mayores frecuencias de eventos perinatales adversos se observaron en madres adolescentes con baja escolaridad. La variable “acceso a servicios de salud” explicó un 35%, 37% y 23% de las disparidades en el bajo peso al nacimiento, prematuridad y retardo de crecimiento intrauterino, respectivamente, entre madres adolescentes con baja y alta escolaridad. El bajo número de consultas prenatales fue el único factor de riesgo para los dos niveles de escolaridad y la variable que mejor explica las diferencias entre las frecuencias de eventos perinatales adversos. Desde el punto de vista de la salud pública, ellos representan una intervención de bajo coste, con posibilidad de que se incrementen mediante información adecuada para la población y medidas sistemáticas en los niveles de atención primaria.
Keywords	Nacimiento Prematuro ; Peso al Nacer ; Embarazo en Adolescencia ; Escolaridad ; Inequidad Social ; Medicine ; R ; Public aspects of medicine ; RA1-1270
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	Escola Nacional de Saúde Pública, Fundação Oswaldo Cruz
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: A graph theory approach to analyze birth defect associations.

Dario Elias / Hebe Campaña / Fernando Poletta / Silvina Heisecke / Juan Gili / Julia Ratowiecki / Lucas Gimenez / Mariela Pawluk / Maria Rita Santos / Viviana Cosentino / Rocio Uranga / Monica Rittler / Jorge Lopez Camelo

PLoS ONE, Vol 15, Iss 5, p e

2020 Volume 0233529

Abstract: Birth defects are prenatal morphological or functional anomalies. Associations among them are studied to identify their etiopathogenesis. The graph theory methods allow analyzing relationships among a complete set of anomalies. A graph consists of nodes ... ...

Abstract	Birth defects are prenatal morphological or functional anomalies. Associations among them are studied to identify their etiopathogenesis. The graph theory methods allow analyzing relationships among a complete set of anomalies. A graph consists of nodes which represent the entities (birth defects in the present work), and edges that join nodes indicating the relationships among them. The aim of the present study was to validate the graph theory methods to study birth defect associations. All birth defects monitoring records from the Estudio Colaborativo Latino Americano de Malformaciones Congénitas gathered between 1967 and 2017 were used. From around 5 million live and stillborn infants, 170,430 had one or more birth defects. Volume-adjusted Chi-Square was used to determine the association strength between two birth defects and to weight the graph edges. The complete birth defect graph showed a Log-Normal degree distribution and its characteristics differed from random, scale-free and small-world graphs. The graph comprised 118 nodes and 550 edges. Birth defects with the highest centrality values were nonspecific codes such as Other upper limb anomalies. After partition, the graph yielded 12 groups; most of them were recognizable and included conditions such as VATER and OEIS associations, and Patau syndrome. Our findings validate the graph theory methods to study birth defect associations. This method may contribute to identify underlying etiopathogeneses as well as to improve coding systems.
Keywords	Medicine ; R ; Science ; Q
Subject code	511
Language	English
Publishing date	2020-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Vasoactive intestinal peptide reduces the inflammatory profile in mice infected with Trypanosoma cruzi

Higyno, Pulchéria Maria Silva / Ana Paula Lucas Mota / Cristiane Alves da Silva Menezes / Dario Elias Pereira / Ivo Santana Caldas / Katiane de Oliveira Pinto Coelho Nogueira / Marina Barcelos de Miranda / Priscila Fagundes Mendes / Sandra Aparecida de Lima Moura

Experimental parasitology. 2015 Dec., v. 159

2015

Abstract: Vasoactive intestinal peptide (VIP) has gained great prominence because of its therapeutic potential, which is ascribed to its ability to regulate innate immunity, inhibit antigen-specific Th1 cell responses, and generate T regulatory cells. Additionally, ...

Abstract	Vasoactive intestinal peptide (VIP) has gained great prominence because of its therapeutic potential, which is ascribed to its ability to regulate innate immunity, inhibit antigen-specific Th1 cell responses, and generate T regulatory cells. Additionally, VIP may act as a natural antimicrobial peptide, killing bacteria, fungi, and infective forms of Trypanosoma brucei. Despite the possible relevance of VIP during the course of Chagas disease, studies regarding this in human and experimental Trypanosoma cruzi infections remain poorly characterized. In this work, we evaluated the effects of VIP on systemic and cardiac immune responses during experimental acute infection. C57BL/6 mice were infected with 5000 trypomastigotes of the VL-10 strain of T. cruzi and treated with intraperitoneal VIP injection every other day for one month. After 30 days, we observed no reduction in parasitemia levels. However, we observed a reduction in serum levels of IFN-gamma and IL-2 and an increase in that of IL-4. These data suggest that VIP treatment modified immune responses to favor the Th2 response, which had no impact on parasitemia levels although the serum level of IFN-gamma was reduced. However, this change in immune balance reduced heart damage, as noted by the smaller cardiac volume and the moderate inflammatory infiltrate observed in VIP-treated mice. Our results indicate that VIP treatment reduced the inflammatory response at the cardiac site of mice that were experimentally infected with T. cruzi. These data suggest a protective role for VIP in the heart of infected mice.
Keywords	acute course ; antimicrobial peptides ; bacteria ; blood serum ; Chagas disease ; fungi ; heart ; humans ; immune response ; infectious diseases ; inflammation ; innate immunity ; interferon-gamma ; interleukin-2 ; interleukin-4 ; mice ; parasitemia ; protective effect ; therapeutics ; Trypanosoma brucei ; Trypanosoma cruzi ; trypomastigotes ; vasoactive intestinal peptide
Language	English
Dates of publication	2015-12
Size	p. 72-78.
Publishing place	Elsevier Inc.
Document type	Article
ZDB-ID	391089-1
ISSN	1090-2449 ; 0014-4894
ISSN (online)	1090-2449
ISSN	0014-4894
DOI	10.1016/j.exppara.2015.09.004
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Vasoactive intestinal peptide reduces the inflammatory profile in mice infected with Trypanosoma cruzi.

Higyno, Pulchéria Maria Silva / Mendes, Priscila Fagundes / Miranda, Marina Barcelos de / Pereira, Dario Elias / Mota, Ana Paula Lucas / Nogueira, Katiane de Oliveira Pinto Coelho / Caldas, Ivo Santana / Moura, Sandra Aparecida de Lima / Menezes, Cristiane Alves da Silva

Experimental parasitology

2015 Volume 159, Page(s) 72–78

Abstract	Vasoactive intestinal peptide (VIP) has gained great prominence because of its therapeutic potential, which is ascribed to its ability to regulate innate immunity, inhibit antigen-specific Th1 cell responses, and generate T regulatory cells. Additionally, VIP may act as a natural antimicrobial peptide, killing bacteria, fungi, and infective forms of Trypanosoma brucei. Despite the possible relevance of VIP during the course of Chagas disease, studies regarding this in human and experimental Trypanosoma cruzi infections remain poorly characterized. In this work, we evaluated the effects of VIP on systemic and cardiac immune responses during experimental acute infection. C57BL/6 mice were infected with 5000 trypomastigotes of the VL-10 strain of T. cruzi and treated with intraperitoneal VIP injection every other day for one month. After 30 days, we observed no reduction in parasitemia levels. However, we observed a reduction in serum levels of IFN-gamma and IL-2 and an increase in that of IL-4. These data suggest that VIP treatment modified immune responses to favor the Th2 response, which had no impact on parasitemia levels although the serum level of IFN-gamma was reduced. However, this change in immune balance reduced heart damage, as noted by the smaller cardiac volume and the moderate inflammatory infiltrate observed in VIP-treated mice. Our results indicate that VIP treatment reduced the inflammatory response at the cardiac site of mice that were experimentally infected with T. cruzi. These data suggest a protective role for VIP in the heart of infected mice.
MeSH term(s)	Animals ; Anti-Inflammatory Agents/therapeutic use ; Chagas Cardiomyopathy/prevention & control ; Chagas Disease/drug therapy ; Chagas Disease/pathology ; Chemokines/analysis ; Cytokines/analysis ; Female ; Mice ; Mice, Inbred C57BL ; Myocardium/pathology ; Neuroprotective Agents/therapeutic use ; Parasitemia/drug therapy ; Parasitemia/parasitology ; Vasoactive Intestinal Peptide/therapeutic use
Chemical Substances	Anti-Inflammatory Agents ; Chemokines ; Cytokines ; Neuroprotective Agents ; Vasoactive Intestinal Peptide (37221-79-7)
Language	English
Publishing date	2015-12
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	391089-1
ISSN	1090-2449 ; 0014-4894
ISSN (online)	1090-2449
ISSN	0014-4894
DOI	10.1016/j.exppara.2015.09.004
Database	MEDical Literature Analysis and Retrieval System OnLINE

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