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Article ; Online: An improved electrochemical aptasensor for chloramphenicol detection based on aptamer incorporated gelatine.

Hamidi-Asl, Ezat / Dardenne, Freddy / Blust, Ronny / De Wael, Karolien

2015 Volume 15, Issue 4, Page(s) 7605–7618

Abstract: Because of the biocompatible properties of gelatine and the good afﬁnity of aptamers for their targets, the combination of aptamer and gelatine type B is reported as promising for the development of biosensing devices. Here, an aptamer for ... ...

Abstract	Because of the biocompatible properties of gelatine and the good afﬁnity of aptamers for their targets, the combination of aptamer and gelatine type B is reported as promising for the development of biosensing devices. Here, an aptamer for chloramphenicol (CAP) is mixed with different types of gelatine and dropped on the surface of disposable gold screen printed electrodes. The signal of the CAP reduction is investigated using differential pulse voltammetry. The diagnostic performance of the sensor is described and a detection limit of 1.83 × 10(-10) M is found. The selectivity and the stability of the aptasensor are studied and compared to those of other CAP sensors described in literature.
MeSH term(s)	Aptamers, Nucleotide/chemistry ; Biosensing Techniques/methods ; Chloramphenicol/chemistry ; Electrochemical Techniques/methods ; Electrodes ; Gelatin/chemistry
Chemical Substances	Aptamers, Nucleotide ; Chloramphenicol (66974FR9Q1) ; Gelatin (9000-70-8)
Language	English
Publishing date	2015-03-27
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2052857-7
ISSN	1424-8220 ; 1424-8220
ISSN (online)	1424-8220
ISSN	1424-8220
DOI	10.3390/s150407605
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Field application of a novel active-passive sampling technique for the simultaneous measurement of a wide range of contaminants in water.

Amato, Elvio D / Pfeiffer, Fabienne / Estoppey, Nicolas / Subotic, Dragan / Herweyers, Laure / Breugelmans, Tom / Weyn, Maarten / Du Bois, Els / Dardenne, Freddy / Covaci, Adrian / Town, Raewyn M / Blust, Ronny

Chemosphere

2021 Volume 279, Page(s) 130598

Abstract: A first test of the field capabilities of a novel in situ sampling technique combining active and passive sampling (APS) was conducted in the sea. The proof-of-concept device uses a pump to draw water into a diffusion cell where dissolved target ... ...

Abstract	A first test of the field capabilities of a novel in situ sampling technique combining active and passive sampling (APS) was conducted in the sea. The proof-of-concept device uses a pump to draw water into a diffusion cell where dissolved target substances are accumulated onto sorbents which are selective for different classes of contaminants (i.e., metal cations, polar and non-polar organic compounds), simultaneously. A controlled laminar flow established in the diffusion cell enables measurements of contaminant concentrations that are fully independent from the hydrodynamic conditions in the bulk solution. APS measurements were consistent with those obtained using conventional passive sampling techniques such as organic diffusive gradients in thin films (o-DGT) and silicone rubber (SR) samplers (generally < 40% difference), taking into account the prevailing hydrodynamic conditions. The use of performance reference compounds (PRC) for hydrophobic contaminants provided additional information. Field measurements of metal ions in seawater showed large variability due to issues related to the device configuration. An improved field set-up deployed in supplementary freshwater mesocosm experiments provided metal speciation data that was consistent with passive sampling measurements (DGT), taking into account the hydrodynamic conditions. Overall, the results indicate that the APS technique provides a promising approach for the determination of a wide range of contaminants simultaneously, and independently from the hydrodynamic conditions in the bulk solution.
MeSH term(s)	Diffusion ; Environmental Monitoring ; Organic Chemicals ; Waste Water/analysis ; Water ; Water Pollutants, Chemical/analysis
Chemical Substances	Organic Chemicals ; Waste Water ; Water Pollutants, Chemical ; Water (059QF0KO0R)
Language	English
Publishing date	2021-04-17
Publishing country	England
Document type	Journal Article
ZDB-ID	120089-6
ISSN	1879-1298 ; 0045-6535 ; 0366-7111
ISSN (online)	1879-1298
ISSN	0045-6535 ; 0366-7111
DOI	10.1016/j.chemosphere.2021.130598
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Article: Field application of a novel active-passive sampling technique for the simultaneous measurement of a wide range of contaminants in water

Chemosphere. 2021 Sept., v. 279

2021

Abstract	A first test of the field capabilities of a novel in situ sampling technique combining active and passive sampling (APS) was conducted in the sea. The proof-of-concept device uses a pump to draw water into a diffusion cell where dissolved target substances are accumulated onto sorbents which are selective for different classes of contaminants (i.e., metal cations, polar and non-polar organic compounds), simultaneously. A controlled laminar flow established in the diffusion cell enables measurements of contaminant concentrations that are fully independent from the hydrodynamic conditions in the bulk solution. APS measurements were consistent with those obtained using conventional passive sampling techniques such as organic diffusive gradients in thin films (o-DGT) and silicone rubber (SR) samplers (generally < 40% difference), taking into account the prevailing hydrodynamic conditions. The use of performance reference compounds (PRC) for hydrophobic contaminants provided additional information. Field measurements of metal ions in seawater showed large variability due to issues related to the device configuration. An improved field set-up deployed in supplementary freshwater mesocosm experiments provided metal speciation data that was consistent with passive sampling measurements (DGT), taking into account the hydrodynamic conditions. Overall, the results indicate that the APS technique provides a promising approach for the determination of a wide range of contaminants simultaneously, and independently from the hydrodynamic conditions in the bulk solution.
Keywords	freshwater ; hydrodynamics ; hydrophobicity ; laminar flow ; rubber ; seawater ; silicone ; sorbents ; water pollution
Language	English
Dates of publication	2021-09
Publishing place	Elsevier Ltd
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	120089-6
ISSN	1879-1298 ; 0045-6535 ; 0366-7111
ISSN (online)	1879-1298
ISSN	0045-6535 ; 0366-7111
DOI	10.1016/j.chemosphere.2021.130598
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Article: Characterization of the accumulation of metals and organic contaminants on a novel active-passive sampling device under controlled water flow conditions

Amato, Elvio D / Bervoets, Lieven / Blust, Ronny / Breugelmans, Tom / Covaci, Adrian / Dardenne, Freddy / Hereijgers, Jonas / Nguyen, Dung T / Subotic, Dragan / Town, Raewyn M / Weyn, Maarten

Chemosphere. 2019 Dec., v. 236

2019

Abstract: Recently, a new sampling device combining active and passive sampling (APS) was developed for the measurement of time-averaged concentrations of metal species and both polar and non-polar organic contaminants in water. By coupling a diffusion cell ( ... ...

Abstract	Recently, a new sampling device combining active and passive sampling (APS) was developed for the measurement of time-averaged concentrations of metal species and both polar and non-polar organic contaminants in water. By coupling a diffusion cell (loaded with a set of sorbents selective for different substances) with a small pump and a flow meter, the APS device is able to perform in situ measurements that are independent of the hydrodynamic conditions in the exposure medium. In the present study, the diffusion layer thickness (δ) at the sorbent/solution interface within the diffusion cell was characterised under controlled flow conditions. Laboratory tests indicated that, in the range of flow rates investigated, the average diffusion layer thickness (δ ̄) varied from ∼60 to ∼110 μm, depending on the type of substance measured and the position of the sorbent with respect to the flow direction. Due to its ability to maintain an approximately constant δ ̄, good to excellent agreement was found between measurements performed with the APS device in non-complexing media and concentrations measured in discrete water samples for all the substances investigated. These results suggest that the APS device could overcome issues affecting the quantitative interpretation of measurements by conventional passive sampling devices and serve as a useful tool for simultaneously monitoring a wide range of contaminants in water.
Keywords	hydrodynamics ; laboratory experimentation ; metals ; monitoring ; sorbents ; water flow ; water pollution
Language	English
Dates of publication	2019-12
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	120089-6
ISSN	1879-1298 ; 0045-6535 ; 0366-7111
ISSN (online)	1879-1298
ISSN	0045-6535 ; 0366-7111
DOI	10.1016/j.chemosphere.2019.124400
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Article ; Online: Assessing in-vitro estrogenic effects of currently-used flame retardants.

Krivoshiev, Boris V / Dardenne, Freddy / Covaci, Adrian / Blust, Ronny / Husson, Steven J

Toxicology in vitro : an international journal published in association with BIBRA

2016 Volume 33, Page(s) 153–162

Abstract: Flame retardants are chemicals that are added to nearly all manufactured materials. Additionally, there has been a steady increase in diseases resulting from endocrine-disruption with an aligned increase in use of chemicals. Given the persistence, ... ...

Abstract	Flame retardants are chemicals that are added to nearly all manufactured materials. Additionally, there has been a steady increase in diseases resulting from endocrine-disruption with an aligned increase in use of chemicals. Given the persistence, potential bioaccumulation, limited toxicological understanding, and vast use of flame retardants, there is a need to investigate potential endocrine-disruptive activity associated with these compounds in an effort for better risk assessment. We therefore used the MCF-7 flow-cytometric proliferation assay in an effort to establish potential estrogen-disrupting effects of twelve currently-used flame retardants. Triphenyl phosphate, tris(1,3-dichloro-2-propyl) phosphate, tris(butyl) phosphate, hexabromocyclododecane, and tetrabromobisphenol A showed statistically significant estrogenic activity, with hexabromocyclododecane being the most potent of the five (EC20 of 5.5 μM). Tris(2-butoxyethyl) phosphate, tris(1,3-dichloro-2-propyl) phosphate, tri(2-chloroethyl) phosphate, tris(butyl) phosphate, hexabromocyclododecane, tetrabromobisphenol A, and tris(2,3,-dibromopropyl) isocyanurate harboured anti-estrogenic activity when co-treating with 17β-estradiol, with hexabromocyclododecane showing the highest potency (IC20 of 17.6 μM). Interestingly, some compounds showed both estrogenic and anti-estrogenic effects, indicating both receptor-dependant and -independent mechanisms attributed to some of these compounds, in line with other studies. Multiple currently-used flame retardants may therefore act as xenoestrogens and anti-estrogens, or alter estrogen homeostasis, which could affect endocrine function.
MeSH term(s)	Cell Proliferation/drug effects ; Cell Survival/drug effects ; Estrogen Antagonists/toxicity ; Estrogens/toxicity ; Flame Retardants/toxicity ; Humans ; Hydrocarbons, Brominated/toxicity ; MCF-7 Cells ; Organophosphates/toxicity
Chemical Substances	Estrogen Antagonists ; Estrogens ; Flame Retardants ; Hydrocarbons, Brominated ; Organophosphates
Language	English
Publishing date	2016-06
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	639064-x
ISSN	1879-3177 ; 0887-2333
ISSN (online)	1879-3177
ISSN	0887-2333
DOI	10.1016/j.tiv.2016.03.006
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Article: A novel active-passive sampling approach for measuring time-averaged concentrations of pollutants in water

Amato, Elvio D / Covaci, Adrian / Town, Raewyn M / Hereijgers, Jonas / Bellekens, Ben / Giacometti, Valentina / Breugelmans, Tom / Weyn, Maarten / Dardenne, Freddy / Bervoets, Lieven / Blust, Ronny

Chemosphere. 2018 Oct., v. 209

2018

Abstract: Passive sampling with in situ devices offers several advantages over traditional sampling methods (i.e., discrete spot sampling), however, data interpretation from conventional passive samplers is hampered by difficulties in estimating the thickness of ... ...

Abstract	Passive sampling with in situ devices offers several advantages over traditional sampling methods (i.e., discrete spot sampling), however, data interpretation from conventional passive samplers is hampered by difficulties in estimating the thickness of the diffusion layer at the sampler/medium interface (δ), often leading to inaccurate determinations of target analyte concentrations. In this study, the performance of a novel device combining active and passive sampling was investigated in the laboratory. The active-passive sampling (APS) device is comprised of a diffusion cell fitted with a pump and a flowmeter. Three receiving phases traditionally used in passive sampling devices (i.e., chelex resin, Oasis HLB, and silicone rubber), were incorporated in the diffusion cell and allowed the simultaneous accumulation of cationic metals, polar, and non-polar organic compounds, respectively. The flow within the diffusion cell was accurately controlled and monitored, and, combined with diffusion coefficients measurements, enabled the average δ to be estimated. Strong agreement between APS and time-averaged total concentrations measured in discrete water samples was found for most of the substances investigated. Accuracies for metals ranged between 87 and 116%, except Cu and Pb (∼50%), whilst accuracies between 64 and 101%, and 92 and 151% were achieved for polar and non-polar organic compounds, respectively. These results indicate that, via a well-defined in situ preconcentration step, the proposed APS approach shows promise for monitoring the concentration of a range of pollutants in water.
Keywords	copper ; diffusivity ; lead ; monitoring ; pollutants ; rubber ; samplers ; sampling ; silicone ; technology
Language	English
Dates of publication	2018-10
Size	p. 363-372.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	120089-6
ISSN	1879-1298 ; 0045-6535 ; 0366-7111
ISSN (online)	1879-1298
ISSN	0045-6535 ; 0366-7111
DOI	10.1016/j.chemosphere.2018.06.079
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Article ; Online: (Electro)Sensing of Phenicol Antibiotics-A Review.

Pilehvar, Sanaz / Gielkens, Kristoffer / Trashin, Stanislav A / Dardenne, Freddy / Blust, Ronny / De Wael, Karolien

Critical reviews in food science and nutrition

2015 Volume 56, Issue 14, Page(s) 2416–2429

Abstract: The presence of residues from frequent antibiotic use in animal feed can cause serious health risks by contaminating products meant for human consumption such as meat and milk. The present paper gives an overview of the electrochemical methods developed ... ...

Abstract	The presence of residues from frequent antibiotic use in animal feed can cause serious health risks by contaminating products meant for human consumption such as meat and milk. The present paper gives an overview of the electrochemical methods developed for the detection of phenicol antibiotic residues (chloramphenicol, thiamphenicol, and florfenicol) in different kinds of foodstuffs. Electrochemical sensors based on different biomolecules and nanomaterials are described. The detection limit of various developed methods with their advantages and disadvantages will be highlighted.
MeSH term(s)	Anti-Bacterial Agents/chemistry ; Anti-Bacterial Agents/classification ; Drug Residues ; Electrochemical Techniques ; Food Analysis ; Humans
Chemical Substances	Anti-Bacterial Agents
Language	English
Publishing date	2015-04-01
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	1037504-1
ISSN	1549-7852 ; 1040-8398
ISSN (online)	1549-7852
ISSN	1040-8398
DOI	10.1080/10408398.2013.845140
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Article ; Online: Elucidating toxicological mechanisms of current flame retardants using a bacterial gene profiling assay.

Krivoshiev, Boris V / Dardenne, Freddy / Blust, Ronny / Covaci, Adrian / Husson, Steven J

Toxicology in vitro : an international journal published in association with BIBRA

2015 Volume 29, Issue 8, Page(s) 2124–2132

Abstract: Flame retardants are ubiquitously used chemicals that have been shown to contaminate environments. Toxicological data is largely limited, with little insight into their molecular modes of action that may give rise to their toxic phenotypes. Such insight ... ...

Abstract	Flame retardants are ubiquitously used chemicals that have been shown to contaminate environments. Toxicological data is largely limited, with little insight into their molecular modes of action that may give rise to their toxic phenotypes. Such insight would aid more effective risk assessments concerning these compounds, while also improving molecular design. We therefore used a bacterial stress-gene profiling assay to screen twelve currently-used flame retardants to obtain mechanistic insights of toxicity. Both brominated and organophosphate flame retardants were tested. All compounds showed statistically significant inductions of several stress genes when compared to control treatments. Triphenyl phosphate, tris(2-butoxyethyl) phosphate, tris(1,3-dichloro-2-propyl)phosphate, tris(butyl)phosphate, and tetrabromobisphenol A elicited (at least) two-fold inductions for any of the stress genes. When looking at absolute induction levels, the promoters induced are indicative of protein perturbation, DNA integrity and membrane integrity. However, normalising for the different induction potentials of the different stress genes and clustering using hierarchical and k-means algorithms indicated that in addition to protein and DNA damage, some compounds also resulted in growth arrest and oxidative damage. This research shows that this assay allows for the determination of toxicological modes-of-action while clustering and accounting for induction potentials of the different genes aids better risk assessment.
MeSH term(s)	Biosensing Techniques/methods ; Cell Proliferation/drug effects ; Escherichia coli/drug effects ; Escherichia coli/metabolism ; Flame Retardants/toxicity ; Gene Expression Regulation, Bacterial/drug effects ; Stress, Physiological/drug effects ; Transcriptome/drug effects
Chemical Substances	Flame Retardants
Language	English
Publishing date	2015-12
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	639064-x
ISSN	1879-3177 ; 0887-2333
ISSN (online)	1879-3177
ISSN	0887-2333
DOI	10.1016/j.tiv.2015.09.001
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Article ; Online: The impact of metal pollution on soil faunal and microbial activity in two grassland ecosystems.

Boshoff, Magdalena / De Jonge, Maarten / Dardenne, Freddy / Blust, Ronny / Bervoets, Lieven

Environmental research

2014 Volume 134, Page(s) 169–180

Abstract: In this study the influence of metal pollution on soil functional activity was evaluated by means of Bait lamina and BIOLOG(®) EcoPlates™ assays. The in situ bait lamina assay investigates the feeding activity of macrofauna, mesofauna and microarthropods ...

Abstract	In this study the influence of metal pollution on soil functional activity was evaluated by means of Bait lamina and BIOLOG(®) EcoPlates™ assays. The in situ bait lamina assay investigates the feeding activity of macrofauna, mesofauna and microarthropods while the BIOLOG(®) EcoPlate™ assay measures the metabolic fingerprint of a selectively extracted microbial community. Both assays proved sensitive enough to reveal changes in the soil community between the plots nearest to and further away from a metal pollution source. Feeding activity (FA) at the less polluted plots reached percentages of 90% while plots nearer to the source of pollution reached percentages as low as 10%. After 2 and 6 days of incubation average well color development (AWCD) and functional richness (R') were significantly lower at the plots closest to the source of pollution. While the Shannon Wiener diversity index (H') decreased significantly at sites nearer to the source of pollution after 2 days but not after 6 days of incubation. Arsenic, Cu and Pb correlated significantly and negatively with feeding activity and functional indices while the role of changing environmental factors such as moisture percentage could not be ruled out completely. Compared to the Bait lamina method that is used in situ and which is therefore more affected by site specific variation, the BIOLOG assay, which excludes confounding factors such as low moisture percentage, may be a more reliable assay to measure soil functional activity.
MeSH term(s)	Ecosystem ; Metals/toxicity ; Poaceae ; Soil Microbiology ; Soil Pollutants/toxicity
Chemical Substances	Metals ; Soil Pollutants
Language	English
Publishing date	2014-10
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	205699-9
ISSN	1096-0953 ; 0013-9351
ISSN (online)	1096-0953
ISSN	0013-9351
DOI	10.1016/j.envres.2014.06.024
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Article ; Online: Carbon nanotubes based electrochemical aptasensing platform for the detection of hydroxylated polychlorinated biphenyl in human blood serum.

Pilehvar, Sanaz / Ahmad Rather, Jahangir / Dardenne, Freddy / Robbens, Johan / Blust, Ronny / De Wael, Karolien

Biosensors & bioelectronics

2014 Volume 54, Page(s) 78–84

Abstract: A novel strategy to sense target molecules in human blood serum is achieved by immobilizing aptamers (APTs) on multi-walled carbon nanotubes (MWCNT) modified electrodes. In this work, the aminated aptamer selected for hydroxylated polychlorinated ... ...

Abstract	A novel strategy to sense target molecules in human blood serum is achieved by immobilizing aptamers (APTs) on multi-walled carbon nanotubes (MWCNT) modified electrodes. In this work, the aminated aptamer selected for hydroxylated polychlorinated biphenyl (OH-PCB) was covalently immobilized on the surface of the MWCNT-COOH modified glassy carbon electrode through amide linkage. The aptamers function as recognition probes for OH-PCB by the binding induced folding of the aptamer. The developed aptasensing device was characterized by electrochemical impedance spectroscopy (EIS), atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR). The aptasensor displayed excellent performance for OH-PCB detection with a linear range from 0.16 to 7.5 μM. The sensitivity of the developed aptasensing platform is improved (1×10(-8) M) compared to the published report (1×10(-6) M) for the determination of OH-PCB (Turner et al., 2007). The better performance of the sensor is due to the unique platform, i.e. the presence of APTs onto electrodes and the combination with nanomaterials. The aptamer density on the electrode surface was estimated by chronocoulometry and was found to be 1.4×10(13) molecules cm(-2). The validity of the method and applicability of the aptasensor was successfully evaluated by the detection of OH-PCB in a blood serum sample. The described approach for aptasensing opens up new perspectives in the field of biomonitoring providing a device with acceptable stability, high sensitivity, good accuracy and precision.
MeSH term(s)	Aptamers, Nucleotide/chemistry ; Biosensing Techniques/instrumentation ; Dielectric Spectroscopy ; Electrochemical Techniques ; Electrodes ; Humans ; Hydroxylation ; Limit of Detection ; Microscopy, Atomic Force ; Nanotubes, Carbon/chemistry ; Polychlorinated Biphenyls/blood
Chemical Substances	Aptamers, Nucleotide ; Nanotubes, Carbon ; Polychlorinated Biphenyls (DFC2HB4I0K)
Language	English
Publishing date	2014-04-15
Publishing country	England
Document type	Evaluation Studies ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1011023-9
ISSN	1873-4235 ; 0956-5663
ISSN (online)	1873-4235
ISSN	0956-5663
DOI	10.1016/j.bios.2013.10.018
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