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  1. Article ; Online: Purification of immune-active macrophage super enhancers by chemical cross-linked chromatin immune precipitation.

    Das, Sonali / Mukherjee, Sohitri / Ali, Nahid

    STAR protocols

    2023  Volume 4, Issue 1, Page(s) 102004

    Abstract: Isolation of extraordinarily long-length super-enhancers (SEs) using typical chromatin immune precipitation (ChIP) techniques can lead to DNA breakage due to uncontrolled cross-linking. We present a redefined ChIP technique for SE purification. After ... ...

    Abstract Isolation of extraordinarily long-length super-enhancers (SEs) using typical chromatin immune precipitation (ChIP) techniques can lead to DNA breakage due to uncontrolled cross-linking. We present a redefined ChIP technique for SE purification. After controlled paraformaldehyde-based cross-linking, glycine was used to quench the cross-linker followed by mild sonication. The sonication produced ideal fragment length of long-length SE chromatin. Presently, miR146a-5p SE of macrophages was pulled using BRD4 protein. Our protocol can reproducibly simplify the SE element isolation issues, in a quality-controlled manner. For complete details on the use and execution of this protocol, please refer to Das et al. (2021).
    MeSH term(s) Nuclear Proteins/metabolism ; Transcription Factors/genetics ; Transcription Factors/metabolism ; Chromatin/genetics ; Macrophages/metabolism ; Enhancer Elements, Genetic ; Immunoprecipitation
    Chemical Substances Nuclear Proteins ; Transcription Factors ; Chromatin
    Language English
    Publishing date 2023-01-11
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2666-1667
    ISSN (online) 2666-1667
    DOI 10.1016/j.xpro.2022.102004
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Limitations and Complications in Treating Femoral Neck Fractures with the Femoral Neck System: A Case Report.

    Kale, Sachin / Chalak, Ajit / Vatkar, Arvind / Dey, Joydeep K / Mehta, Nrupam / Das, Sonali

    Journal of orthopaedic case reports

    2024  Volume 14, Issue 3, Page(s) 78–82

    Abstract: Introduction: The femoral neck system (FNS) represents an emerging fixation system designed for the management of femoral neck fractures. This innovative system combines the mechanical benefits of compression and anti-rotation properties in internal ... ...

    Abstract Introduction: The femoral neck system (FNS) represents an emerging fixation system designed for the management of femoral neck fractures. This innovative system combines the mechanical benefits of compression and anti-rotation properties in internal fixation. Biomechanical studies have demonstrated the superior axial and rotational stability of the FNS implant when compared to traditional cannulated screws and dynamic hip screw. Despite these promising mechanical advantages, there is currently a limited body of literature addressing the factors contributing to FNS failure. A thorough assessment of the safety and outcomes associated with this novel implant is essential.
    Case report: In this context, we present three cases wherein FNS failure occurred, aiming to elucidate the potential causes behind these failures, and had to undergo either an implant removal or bipolar hemiarthroplasty.
    Conclusion: While considering the femoral neck system as the implant of choice, we should consider the age, injury to surgery time, and the location of the fracture line. However, we cannot conclusively ascertain whether age contributes independently to the risk of failure.
    Language English
    Publishing date 2024-03-15
    Publishing country India
    Document type Case Reports
    ZDB-ID 2658169-3
    ISSN 2250-0685
    ISSN 2250-0685
    DOI 10.13107/jocr.2024.v14.i03.4294
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: The paradigm of intracellular parasite survival and drug resistance in leishmanial parasite through genome plasticity and epigenetics: Perception and future perspective.

    Kamran, Mohd / Bhattacharjee, Rahul / Das, Sonali / Mukherjee, Sohitri / Ali, Nahid

    Frontiers in cellular and infection microbiology

    2023  Volume 13, Page(s) 1001973

    Abstract: ... ...

    Abstract Leishmania
    MeSH term(s) Animals ; Parasites ; Leishmania donovani/genetics ; DNA Copy Number Variations ; Drug Resistance ; Perception
    Language English
    Publishing date 2023-02-06
    Publishing country Switzerland
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 2619676-1
    ISSN 2235-2988 ; 2235-2988
    ISSN (online) 2235-2988
    ISSN 2235-2988
    DOI 10.3389/fcimb.2023.1001973
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Nonoxidative Coupling of Methane over Ceria-Supported Single-Atom Pt Catalysts in DBD Plasma.

    Liu, Lina / Das, Sonali / Zhang, Zhikun / Kawi, Sibudjing

    ACS applied materials & interfaces

    2022  Volume 14, Issue 4, Page(s) 5363–5375

    Abstract: Plasma-catalytic direct nonoxidative coupling of methane (NCM) into ... ...

    Abstract Plasma-catalytic direct nonoxidative coupling of methane (NCM) into C
    Language English
    Publishing date 2022-01-24
    Publishing country United States
    Document type Journal Article
    ISSN 1944-8252
    ISSN (online) 1944-8252
    DOI 10.1021/acsami.1c21550
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Nonoxidative Coupling of Methane over Ceria-Supported Single-Atom Pt Catalysts in DBD Plasma

    Liu, Lina / Das, Sonali / Zhang, Zhikun / Kawi, Sibudjing

    ACS applied materials & interfaces. 2022 Jan. 24, v. 14, no. 4

    2022  

    Abstract: Plasma-catalytic direct nonoxidative coupling of methane (NCM) into C₂ hydrocarbons was investigated over ceria-supported atomically dispersed Pt (Pt/CeO₂-SAC) and nanoparticle Pt (Pt/CeO₂-NP) catalysts in dielectric barrier discharge (DBD) plasma. ... ...

    Abstract Plasma-catalytic direct nonoxidative coupling of methane (NCM) into C₂ hydrocarbons was investigated over ceria-supported atomically dispersed Pt (Pt/CeO₂-SAC) and nanoparticle Pt (Pt/CeO₂-NP) catalysts in dielectric barrier discharge (DBD) plasma. Nonthermal plasma facilitated C–H bond dissociation in CH₄ at low temperatures (<150 °C) and atmospheric pressure. The presence of Pt/CeO₂ catalysts in plasma further enhanced CH₄ conversion and C₂ hydrocarbon selectivity by enabling the conversion of vibrationally excited methane species with high internal energy on active Pt sites. Noticeably, the Pt/CeO₂-SAC catalyst displayed a more remarkable performance, with a CH₄ conversion of 39% and a C₂ selectivity of 54% at 54 W. The enhanced CH₄ conversion was attributed to abundant coordinatively unsaturated Pt sites in Pt/CeO₂-SAC, which were more active for C–H bond scission. Meanwhile, isolated Pt atoms in Pt/CeO₂-SAC promoted C₂ hydrocarbon formation by hindering the unselective formation of coke from deep dehydrogenation of CHₓ• intermediates and higher hydrocarbons from oligomerization reactions.
    Keywords atmospheric pressure ; catalysts ; cleavage (chemistry) ; dehydrogenation ; dissociation ; energy ; methane ; oligomerization
    Language English
    Dates of publication 2022-0124
    Size p. 5363-5375.
    Publishing place American Chemical Society
    Document type Article
    ISSN 1944-8252
    DOI 10.1021/acsami.1c21550
    Database NAL-Catalogue (AGRICOLA)

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  6. Book ; Online: The effect of leverage on asset sales between financial institutions

    Das, Sonali

    (IMF working paper ; WP/17, 200)

    2017  

    Author's details by Sonali Das
    Series title IMF working paper ; WP/17, 200
    Keywords Finanzkrise ; Kapitalstruktur ; Finanzsektor ; Bilanz ; Investitionsentscheidung
    Language English
    Size 1 Online-Ressource (circa 18 Seiten)
    Publisher International Monetary Fund
    Publishing place Washington, D.C.
    Document type Book ; Online
    ISBN 9781484318171 ; 148431817X
    DOI 10.5089/9781484318171.001
    Database ECONomics Information System

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  7. Article ; Online: Super enhancer-mediated transcription of miR146a-5p drives M2 polarization during Leishmania donovani infection.

    Das, Sonali / Mukherjee, Sohitri / Ali, Nahid

    PLoS pathogens

    2021  Volume 17, Issue 2, Page(s) e1009343

    Abstract: The outcome of Leishmania donovani infection depends upon the dynamic interchanges between M1 and M2 macrophages. Information of the involvement of microRNAs (miRNAs) and epigenetic modifiers in regulating macrophage plasticity during L. donovani ... ...

    Abstract The outcome of Leishmania donovani infection depends upon the dynamic interchanges between M1 and M2 macrophages. Information of the involvement of microRNAs (miRNAs) and epigenetic modifiers in regulating macrophage plasticity during L. donovani infection is still elusive. Differential expression analysis of polarization-regulating miRNAs, revealed significant enrichment of miR146a-5p during Leishmania donovani infection. A sustained enrichment of miR146a-5p was observed in both infected bone marrow derived macrophages (BMDMs) and BALB/c mice organs. We found involvement of miR146a-5p in phagocytosis and survivability of parasites. Moreover, miR146a-5pgot enriched in interleukin 4- stimulated BMDMs, indicating its possible involvement in M2 polarization. Upon transfecting BMDMs with miRVANA anti-146a oligos, M2 markers (CCR7, YM-1, FIZZ-1, arginase-1, IL10 and IL4) and transcription factors (p-STAT6 and c/EBPβ) got depleted with concomitant augmentation of M1-polarizing transcription factors (p-STAT1, AP1 and IRF-1), miR146a target genes (TRAF6 and IRAK1), M1 cytokines (IL12 and TNFα), iNOS, nitric oxide, and nuclear translocation of phospho p-65 subunit. Neutralization of intracellular mature miR146a-5p pool in infected BALB/c mice lower organ parasite burden and expressions of M2 markers and IL10 with enrichment of M1 markers like iNOS and IL12. Additionally, we explored the novel role of super enhancer (SE), a cis-acting regulatory component, to enrich miR146a-5p expression during infection. Enhanced expression and nuclear retention of SE components like BET bromodomain 4 (BRD4) and p300 were found in infected BMDMs. Upon silencing BRD4, expressions of miR146a-5p and M2 markers were down regulated and TRAF6, IRAK1 and iNOS levels increased. STRING V.11 based predication and immune precipitation confirmed the strong interaction amongst BRD4, p300 and RNA pol II (RpbI). Chromatin immune precipitation studies suggested the recruitment of BRD4 at the enhancer loci of miR146a-5p gene during infection. Altogether, our findings revealed a novel role of BRD4/p300-depdendent super-enhancer in regulating miR146a expression during L. donovani infection which in turn mediates M2 polarization and immune-suppression.
    MeSH term(s) Animals ; Enhancer Elements, Genetic ; Interleukin-1 Receptor-Associated Kinases/genetics ; Interleukin-1 Receptor-Associated Kinases/metabolism ; Leishmania donovani/physiology ; Leishmaniasis/genetics ; Leishmaniasis/immunology ; Leishmaniasis/metabolism ; Leishmaniasis/parasitology ; Macrophages/immunology ; Macrophages/metabolism ; Mice ; Mice, Inbred BALB C ; MicroRNAs/genetics ; Nitric Oxide Synthase Type II/genetics ; Nitric Oxide Synthase Type II/metabolism ; Phagocytosis ; STAT6 Transcription Factor/genetics ; STAT6 Transcription Factor/metabolism
    Chemical Substances MicroRNAs ; Mirn146 microRNA, mouse ; STAT6 Transcription Factor ; Stat6 protein, mouse ; Nitric Oxide Synthase Type II (EC 1.14.13.39) ; Nos2 protein, mouse (EC 1.14.13.39) ; Interleukin-1 Receptor-Associated Kinases (EC 2.7.11.1) ; Irak1 protein, mouse (EC 2.7.11.1)
    Language English
    Publishing date 2021-02-25
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2205412-1
    ISSN 1553-7374 ; 1553-7374
    ISSN (online) 1553-7374
    ISSN 1553-7374
    DOI 10.1371/journal.ppat.1009343
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: COVID-19 PREDICTION IN SOUTH AFRICA: ESTIMATING THE UNASCERTAINED CASES- THE HIDDEN PART OF THE EPIDEMIOLOGICAL ICEBERG.

    Gu, Xuelin / Mukherjee, Bhramar / Das, Sonali / Datta, Jyotishka

    medRxiv : the preprint server for health sciences

    2021  

    Abstract: Understanding the impact of non-pharmaceutical interventions as well as accounting for the unascertained cases remain critical challenges for epidemiological models for understanding the transmission dynamics of COVID-19 spread. In this paper, we propose ...

    Abstract Understanding the impact of non-pharmaceutical interventions as well as accounting for the unascertained cases remain critical challenges for epidemiological models for understanding the transmission dynamics of COVID-19 spread. In this paper, we propose a new epidemiological model (eSEIRD) that extends the widely used epidemiological models such as extended Susceptible-Infected-Removed model (eSIR) and SAPHIRE (initially developed and used for analyzing data from Wuhan). We fit these models to the daily ascertained infected (and removed) cases from March 15, 2020 to Dec 31, 2020 in South Africa that reported the largest number of confirmed COVID-19 cases and deaths from the WHO African region. Using the eSEIRD model, the COVID-19 transmission dynamics in South Africa was characterized by the estimated basic reproduction number (
    Language English
    Publishing date 2021-03-05
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2020.12.10.20247361
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Long COVID? Prospects for economic scarring from the pandemic.

    Barrett, Philip / Das, Sonali / Magistretti, Giacomo / Pugacheva, Evgenia / Wingender, Philippe

    Contemporary economic policy

    2022  

    Abstract: This paper examines the potential persistent effects (scarring) of the COVID-19 pandemic on the economy and the channels through which they may occur. Our findings from a broad set of historical recessions confirm that recessions are associated with ... ...

    Abstract This paper examines the potential persistent effects (scarring) of the COVID-19 pandemic on the economy and the channels through which they may occur. Our findings from a broad set of historical recessions confirm that recessions are associated with persistent output losses and that the greatest scarring has occurred following financial crises. The amount of scarring following pandemic and epidemic recessions in the sample is in between that of typical recessions and financial crises. Results on the channels show that the productivity channel is important, as all types of recessions have been followed by persistent losses to total factor productivity.
    Language English
    Publishing date 2022-11-12
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2012949-X
    ISSN 1465-7287 ; 1074-3529
    ISSN (online) 1465-7287
    ISSN 1074-3529
    DOI 10.1111/coep.12598
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  10. Article ; Online: Ethene Hydroformylation Catalyzed by Rhodium Dispersed with Zinc or Cobalt in Silanol Nests of Dealuminated Zeolite Beta.

    Qi, Liang / Das, Sonali / Zhang, Yanfei / Nozik, Danna / Gates, Bruce C / Bell, Alexis T

    Journal of the American Chemical Society

    2023  Volume 145, Issue 5, Page(s) 2911–2929

    Abstract: Catalysts for hydroformylation of ethene were prepared by grafting Rh into nests of ≡SiOZn-OH or ≡SiOCo-OH species prepared in dealuminated BEA zeolite. X-ray absorption spectra and infrared spectra of adsorbed CO were used to characterize the dispersion ...

    Abstract Catalysts for hydroformylation of ethene were prepared by grafting Rh into nests of ≡SiOZn-OH or ≡SiOCo-OH species prepared in dealuminated BEA zeolite. X-ray absorption spectra and infrared spectra of adsorbed CO were used to characterize the dispersion of Rh. The Rh dispersion was found to increase markedly with increasing M/Rh (M = Zn or Co) ratio; further increases in Rh dispersion occurred upon use for ethene hydroformylation catalysis. The turnover frequency for ethene hydroformylation measured for a fixed set of reaction conditions increased with the fraction of atomically dispersed Rh. The ethene hydroformylation activity is 15.5-fold higher for M = Co than for M = Zn, whereas the propanal selectivity is slightly greater for the latter catalyst. The activity of the Co-containing catalyst exceeds that of all previously reported Rh-containing bimetallic catalysts. The rates of ethene hydroformylation and ethene hydrogenation exhibit positive reaction orders in ethene and hydrogen but negative orders in carbon monoxide.
    Language English
    Publishing date 2023-01-30
    Publishing country United States
    Document type Journal Article
    ZDB-ID 3155-0
    ISSN 1520-5126 ; 0002-7863
    ISSN (online) 1520-5126
    ISSN 0002-7863
    DOI 10.1021/jacs.2c11075
    Database MEDical Literature Analysis and Retrieval System OnLINE

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