LIVIVO - Search results -

Search results

Result 1 - 8 of total 8

Search options

Article ; Online: A bumpy road ahead for genetic biocontainment.

George, Dalton R / Danciu, Mark / Davenport, Peter W / Lakin, Matthew R / Chappell, James / Frow, Emma K

2024 Volume 15, Issue 1, Page(s) 650

Language	English
Publishing date	2024-01-20
Publishing country	England
Document type	Journal Article
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-023-44531-1
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article ; Online: Quorum Sensing Is Accompanied by Global Metabolic Changes in the Opportunistic Human Pathogen Pseudomonas aeruginosa.

Davenport, Peter W / Griffin, Julian L / Welch, Martin

Journal of bacteriology

2015 Volume 197, Issue 12, Page(s) 2072–2082

Abstract: Unlabelled: Pseudomonas aeruginosa uses N-acyl-homoserine lactone (AHL)-dependent quorum sensing (QS) systems to control the expression of secreted effectors. These effectors can be crucial to the ecological fitness of the bacterium, playing roles in ... ...

Abstract	Unlabelled: Pseudomonas aeruginosa uses N-acyl-homoserine lactone (AHL)-dependent quorum sensing (QS) systems to control the expression of secreted effectors. These effectors can be crucial to the ecological fitness of the bacterium, playing roles in nutrient acquisition, microbial competition, and virulence. In this study, we investigated the metabolic consequences of AHL-dependent QS by monitoring the metabolic profile(s) of a lasI rhlI double mutant (unable to make QS signaling molecules) and its wild-type progenitor as they progressed through the growth curve. Analysis of culture supernatants by (1)H-nuclear magnetic resonance ((1)H-NMR) spectroscopy revealed that at the point where AHL concentrations peaked in the wild type, the metabolic footprints (i.e., extracellular metabolites) of the wild-type and lasI rhlI mutant diverged. Subsequent gas chromatography-mass spectrometry (GC-MS)-based analysis of the intracellular metabolome revealed QS-dependent perturbations in around one-third of all identified metabolites, including altered concentrations of tricarboxylic acid (TCA) cycle intermediates, amino acids, and fatty acids. Further targeted fatty acid methyl ester (FAME) GC-MS-based profiling of the cellular total fatty acid pools revealed that QS leads to changes associated with decreased membrane fluidity and higher chemical stability. However, not all of the changes we observed were necessarily a direct consequence of QS; liquid chromatography (LC)-MS analyses revealed that polyamine levels were elevated in the lasI rhlI mutant, perhaps a response to the absence of QS-dependent adaptations. Our data suggest that QS leads to a global readjustment in central metabolism and provide new insight into the metabolic changes associated with QS during stationary-phase adaptation. Importance: Quorum sensing (QS) is a transcriptional regulatory mechanism that allows bacteria to coordinate their gene expression profile with the population cell density. The opportunistic human pathogen Pseudomonas aeruginosa uses QS to control the production of secreted virulence factors. In this study, we show that QS elicits a global "metabolic rewiring" in P. aeruginosa. This metabolic rerouting of fluxes is consistent with a variety of drivers, ranging from altered QS-dependent transcription of "metabolic genes" through to the effect(s) of global "metabolic readjustment" as a consequence of QS-dependent exoproduct synthesis, as well as a general stress response, among others. To our knowledge, this is the first study of its kind to assess the global impact of QS on the metabolome.
MeSH term(s)	Acyl-Butyrolactones/metabolism ; Alanine/metabolism ; Amino Acids/metabolism ; Energy Metabolism/physiology ; Fatty Acids/metabolism ; Gene Expression Regulation, Bacterial/physiology ; Mutation ; Polyamines/metabolism ; Principal Component Analysis ; Pseudomonas aeruginosa/metabolism ; Pseudomonas aeruginosa/physiology ; Quorum Sensing/physiology
Chemical Substances	Acyl-Butyrolactones ; Amino Acids ; Fatty Acids ; Polyamines ; Alanine (OF5P57N2ZX)
Language	English
Publishing date	2015-04-13
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2968-3
ISSN	1098-5530 ; 0021-9193
ISSN (online)	1098-5530
ISSN	0021-9193
DOI	10.1128/JB.02557-14
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Ud II Zs.50: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Quorum Sensing Is Accompanied by Global Metabolic Changes in the Opportunistic Human Pathogen Pseudomonas aeruginosa

Davenport, Peter W / Griffin, Julian L / Welch, Martin

Journal of bacteriology. 2015 June 15, v. 197, no. 12

2015

Abstract: Pseudomonas aeruginosa uses N -acyl-homoserine lactone (AHL)-dependent quorum sensing (QS) systems to control the expression of secreted effectors. These effectors can be crucial to the ecological fitness of the bacterium, playing roles in nutrient ... ...

Abstract	Pseudomonas aeruginosa uses N -acyl-homoserine lactone (AHL)-dependent quorum sensing (QS) systems to control the expression of secreted effectors. These effectors can be crucial to the ecological fitness of the bacterium, playing roles in nutrient acquisition, microbial competition, and virulence. In this study, we investigated the metabolic consequences of AHL-dependent QS by monitoring the metabolic profile(s) of a lasI rhlI double mutant (unable to make QS signaling molecules) and its wild-type progenitor as they progressed through the growth curve. Analysis of culture supernatants by ¹H-nuclear magnetic resonance (¹H-NMR) spectroscopy revealed that at the point where AHL concentrations peaked in the wild type, the metabolic footprints (i.e., extracellular metabolites) of the wild-type and lasI rhlI mutant diverged. Subsequent gas chromatography-mass spectrometry (GC-MS)-based analysis of the intracellular metabolome revealed QS-dependent perturbations in around one-third of all identified metabolites, including altered concentrations of tricarboxylic acid (TCA) cycle intermediates, amino acids, and fatty acids. Further targeted fatty acid methyl ester (FAME) GC-MS-based profiling of the cellular total fatty acid pools revealed that QS leads to changes associated with decreased membrane fluidity and higher chemical stability. However, not all of the changes we observed were necessarily a direct consequence of QS; liquid chromatography (LC)-MS analyses revealed that polyamine levels were elevated in the lasI rhlI mutant, perhaps a response to the absence of QS-dependent adaptations. Our data suggest that QS leads to a global readjustment in central metabolism and provide new insight into the metabolic changes associated with QS during stationary-phase adaptation. IMPORTANCE Quorum sensing (QS) is a transcriptional regulatory mechanism that allows bacteria to coordinate their gene expression profile with the population cell density. The opportunistic human pathogen Pseudomonas aeruginosa uses QS to control the production of secreted virulence factors. In this study, we show that QS elicits a global “metabolic rewiring” in P. aeruginosa . This metabolic rerouting of fluxes is consistent with a variety of drivers, ranging from altered QS-dependent transcription of “metabolic genes” through to the effect(s) of global “metabolic readjustment” as a consequence of QS-dependent exoproduct synthesis, as well as a general stress response, among others. To our knowledge, this is the first study of its kind to assess the global impact of QS on the metabolome.
Keywords	Pseudomonas aeruginosa ; amino acids ; bacteria ; fatty acid methyl esters ; fatty acids ; gas chromatography-mass spectrometry ; gene expression ; genes ; humans ; liquid chromatography ; membrane fluidity ; metabolism ; metabolites ; metabolome ; microbial competition ; monitoring ; mutants ; nuclear magnetic resonance spectroscopy ; pathogens ; polyamines ; quorum sensing ; stress response ; transcription (genetics) ; virulence
Language	English
Dates of publication	2015-0615
Size	p. 2072-2082.
Publishing place	American Society for Microbiology
Document type	Article
ZDB-ID	2968-3
ISSN	1098-5530 ; 0021-9193
ISSN (online)	1098-5530
ISSN	0021-9193
DOI	10.1128/JB.02557-14
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 4' 62/105: Show issues
Z 487: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Analysis of chloroformate-derivatised amino acids, dipeptides and polyamines by LC-MS/MS.

Ubhi, Baljit K / Davenport, Peter W / Welch, Martin / Riley, John / Griffin, Julian L / Connor, Susan C

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

2013 Volume 934, Page(s) 79–88

Abstract: A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed which, with sample preparation using a commercially available kit, allows rapid quantitation of 39 chloroformate-derivatised amino acids (AAs), polyamines (PAs) and ... ...

Abstract	A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed which, with sample preparation using a commercially available kit, allows rapid quantitation of 39 chloroformate-derivatised amino acids (AAs), polyamines (PAs) and dipeptides (DPs) in complex biological matrices. Lower limits of quantitation (LOQ) were 20-150nM for putrescine, spermine, spermidine, cadaverine, agmatine, and below 5μM for all analytes. Responses were linear for all analytes between 0.5 and 50μM. Quantitative measurements of all 39 metabolites were achieved within a 15min runtime. The method was evaluated with a Pseudomonas aeruginosa cell extract study (n=24) and a larger human urine study (n=308). Batch effects were observed in the urine study and an investigation of instrument and sample stability showed a wave-like pattern in the MS responses. Both the run order and inter-batch variation were successfully corrected by normalising to pooled urine quality control data. Thus, this method should be suitable for diverse biological matrices and for large as well as small sample sets.
MeSH term(s)	Amino Acids/chemistry ; Amino Acids/urine ; Chromatography, Liquid/methods ; Dipeptides/chemistry ; Dipeptides/urine ; Formates/chemistry ; Humans ; Polyamines/chemistry ; Polyamines/urine ; Pseudomonas aeruginosa/chemistry ; Pseudomonas aeruginosa/metabolism ; Tandem Mass Spectrometry/methods
Chemical Substances	Amino Acids ; Dipeptides ; Formates ; Polyamines ; chlorocarbonic acid (463-73-0)
Language	English
Publishing date	2013-06-29
Publishing country	Netherlands
Document type	Evaluation Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1180823-8
ISSN	1873-376X ; 0378-4347 ; 1570-0232 ; 1387-2273
ISSN (online)	1873-376X
ISSN	0378-4347 ; 1570-0232 ; 1387-2273
DOI	10.1016/j.jchromb.2013.06.026
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 813: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Escherichia coli flagellar genes as target sites for integration and expression of genetic circuits.

Juhas, Mario / Evans, Lewis D B / Frost, Joe / Davenport, Peter W / Yarkoni, Orr / Fraser, Gillian M / Ajioka, James W

PloS one

2014 Volume 9, Issue 10, Page(s) e111451

Abstract: E. coli is a model platform for engineering microbes, so genetic circuit design and analysis will be greatly facilitated by simple and effective approaches to introduce genetic constructs into the E. coli chromosome at well-characterised loci. We ... ...

Abstract	E. coli is a model platform for engineering microbes, so genetic circuit design and analysis will be greatly facilitated by simple and effective approaches to introduce genetic constructs into the E. coli chromosome at well-characterised loci. We combined the Red recombinase system of bacteriophage λ and Isothermal Gibson Assembly for rapid integration of novel DNA constructs into the E. coli chromosome. We identified the flagellar region as a promising region for integration and expression of genetic circuits. We characterised integration and expression at four candidate loci, fliD, fliS, fliT, and fliY, of the E. coli flagellar region 3a. The integration efficiency and expression from the four integrations varied considerably. Integration into fliD and fliS significantly decreased motility, while integration into fliT and fliY had only a minor effect on the motility. None of the integrations had negative effects on the growth of the bacteria. Overall, we found that fliT was the most suitable integration site.
MeSH term(s)	Bacterial Proteins/genetics ; Bacteriophage lambda/metabolism ; Chromosomes, Bacterial ; DNA, Bacterial/metabolism ; Drug Resistance, Bacterial ; Escherichia coli/metabolism ; Escherichia coli Proteins/metabolism ; Flagella/metabolism ; Flagellin/genetics ; Gene Expression Regulation, Bacterial ; Gene Regulatory Networks ; Green Fluorescent Proteins/metabolism ; Membrane Proteins/genetics ; Molecular Chaperones/genetics ; Mutation ; Open Reading Frames ; Polymerase Chain Reaction ; Recombinases/metabolism ; Synthetic Biology
Chemical Substances	Bacterial Proteins ; DNA, Bacterial ; Escherichia coli Proteins ; Membrane Proteins ; Molecular Chaperones ; Recombinases ; Flagellin (12777-81-0) ; Green Fluorescent Proteins (147336-22-9) ; FlaD protein, Bacteria (147757-14-0) ; FliS protein, Bacteria (147757-15-1) ; fliT protein, Bacteria (147757-16-2) ; FliY protein, Bacteria (148997-36-8)
Language	English
Publishing date	2014-10-28
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0111451
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Mutation of nfxB causes global changes in the physiology and metabolism of Pseudomonas aeruginosa.

Stickland, Hannah G / Davenport, Peter W / Lilley, Kathryn S / Griffin, Julian L / Welch, Martin

Journal of proteome research

2010 Volume 9, Issue 6, Page(s) 2957–2967

Abstract: Loss-of-function mutations in nfxB lead to up-regulation of mexCD-oprJ expression and, consequently, increased resistance to fluoroquinolone antibiotics. Such nfxB mutants have also been reported to exhibit altered virulence profiles, diminished type III ...

Abstract	Loss-of-function mutations in nfxB lead to up-regulation of mexCD-oprJ expression and, consequently, increased resistance to fluoroquinolone antibiotics. Such nfxB mutants have also been reported to exhibit altered virulence profiles, diminished type III secretion system-dependent cytotoxicity, and impaired fitness. However, it is not clear whether these phenotypes are directly linked to NfxB activity or whether inappropriate expression of the MexCD-OprJ pump has pleiotropic effects, thereby impacting indirectly on the phenotype of the cells. The aim of the current work is to investigate which of these possibilities is correct. We isolated a novel type of nfxB mutant generated by a spontaneous polygenic deletion and show that this mutant is rapidly out-competed when grown in a mixed culture with the wild-type progenitor. This competitive fitness defect only manifested itself during the stationary phase of growth. The endoproteome of the nfxB mutant, assessed using 2D-DiGE (difference gel electrophoresis), showed major alterations compared with the wild-type. Consistent with this, we found that the nfxB mutant was impaired in all forms of motility (swimming, swarming, and twitching) as well as in the production of siderophores, rhamnolipid, secreted protease, and pyocyanin. Further investigation showed that the exoproteome, endometabolome, and exometabolome of the nfxB mutant were all globally different compared with the wild-type. The exometabolome of the nfxB mutant was enriched in a selection of long chain fatty acids raising the possibility that these might be substrates for the MexCD-OprJ pump. The nfxB mutant metabotype could be complemented by expression of nfxB in trans and was abolished in an nfxB mexD double mutant, suggesting that inappropriate overexpression of a functional MexCD-OprJ efflux pump causes pleiotropic changes. Taken together, our data suggest that many of the nfxB mutant phenotypes are not caused by the direct effects of the NfxB regulator, but instead by inappropriate mexCD-oprJ expression. Furthermore, the pleiotropic nature of the phenotypes indicate that these may simply reflect the globally dysregulated physiology of the strain.
MeSH term(s)	Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Bacterial Proteins/physiology ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; DNA-Binding Proteins/physiology ; Electrophoresis, Gel, Two-Dimensional ; Membrane Transport Proteins/metabolism ; Metabolome ; Mutation ; Phenotype ; Principal Component Analysis ; Proteome ; Pseudomonas aeruginosa/genetics ; Pseudomonas aeruginosa/metabolism ; Pseudomonas aeruginosa/physiology ; Transcription Factors/genetics ; Transcription Factors/metabolism ; Transcription Factors/physiology
Chemical Substances	Bacterial Proteins ; DNA-Binding Proteins ; Membrane Transport Proteins ; NfxB protein, Pseudomonas aeruginosa ; Proteome ; Transcription Factors
Language	English
Publishing date	2010-04-04
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2078618-9
ISSN	1535-3907 ; 1535-3893
ISSN (online)	1535-3907
ISSN	1535-3893
DOI	10.1021/pr9011415
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 6189: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Quorum Sensing Is Accompanied by Global Metabolic Changes in the Opportunistic Human Pathogen Pseudomonas aeruginosa

Davenport, Peter W. / Griffin, Julian L. / Welch, Martin

Journal of bacteriology

Volume v. 197,, Issue no. 1

Abstract	Pseudomonas aeruginosa uses N -acyl-homoserine lactone (AHL)-dependent quorum sensing (QS) systems to control the expression of secreted effectors. These effectors can be crucial to the ecological fitness of the bacterium, playing roles in nutrient acquisition, microbial competition, and virulence. In this study, we investigated the metabolic consequences of AHL-dependent QS by monitoring the metabolic profile(s) of a lasI rhlI double mutant (unable to make QS signaling molecules) and its wild-type progenitor as they progressed through the growth curve. Analysis of culture supernatants by ¹H-nuclear magnetic resonance (¹H-NMR) spectroscopy revealed that at the point where AHL concentrations peaked in the wild type, the metabolic footprints (i.e., extracellular metabolites) of the wild-type and lasI rhlI mutant diverged. Subsequent gas chromatography-mass spectrometry (GC-MS)-based analysis of the intracellular metabolome revealed QS-dependent perturbations in around one-third of all identified metabolites, including altered concentrations of tricarboxylic acid (TCA) cycle intermediates, amino acids, and fatty acids. Further targeted fatty acid methyl ester (FAME) GC-MS-based profiling of the cellular total fatty acid pools revealed that QS leads to changes associated with decreased membrane fluidity and higher chemical stability. However, not all of the changes we observed were necessarily a direct consequence of QS; liquid chromatography (LC)-MS analyses revealed that polyamine levels were elevated in the lasI rhlI mutant, perhaps a response to the absence of QS-dependent adaptations. Our data suggest that QS leads to a global readjustment in central metabolism and provide new insight into the metabolic changes associated with QS during stationary-phase adaptation. IMPORTANCE Quorum sensing (QS) is a transcriptional regulatory mechanism that allows bacteria to coordinate their gene expression profile with the population cell density. The opportunistic human pathogen Pseudomonas aeruginosa uses QS to control the production of secreted virulence factors. In this study, we show that QS elicits a global “metabolic rewiring” in P. aeruginosa . This metabolic rerouting of fluxes is consistent with a variety of drivers, ranging from altered QS-dependent transcription of “metabolic genes” through to the effect(s) of global “metabolic readjustment” as a consequence of QS-dependent exoproduct synthesis, as well as a general stress response, among others. To our knowledge, this is the first study of its kind to assess the global impact of QS on the metabolome.
Keywords	liquid chromatography ; metabolites ; monitoring ; fatty acids ; quorum sensing ; genes ; metabolism ; gas chromatography-mass spectrometry ; humans ; pathogens ; transcription (genetics) ; stress response ; mutants ; metabolome ; microbial competition ; polyamines ; amino acids ; bacteria ; fatty acid methyl esters ; virulence ; membrane fluidity ; gene expression ; Pseudomonas aeruginosa ; nuclear magnetic resonance spectroscopy
Language	English
Document type	Article
ISSN	0021-9193
Database	AGRIS - International Information System for the Agricultural Sciences and Technology

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Analysis of chloroformate-derivatised amino acids, dipeptides and polyamines by LC–MS/MS

Ubhi, Baljit K. / Davenport, Peter W. / Welch, Martin / Riley, John / Griffin, Julian L. / Connor, Susan C.

Journal of chromatography

Volume v. 934

Abstract: A liquid chromatography–tandem mass spectrometry (LC–MS/MS) method was developed which, with sample preparation using a commercially available kit, allows rapid quantitation of 39 chloroformate-derivatised amino acids (AAs), polyamines (PAs) and ... ...

Abstract	A liquid chromatography–tandem mass spectrometry (LC–MS/MS) method was developed which, with sample preparation using a commercially available kit, allows rapid quantitation of 39 chloroformate-derivatised amino acids (AAs), polyamines (PAs) and dipeptides (DPs) in complex biological matrices. Lower limits of quantitation (LOQ) were 20–150nM for putrescine, spermine, spermidine, cadaverine, agmatine, and below 5μM for all analytes. Responses were linear for all analytes between 0.5 and 50μM. Quantitative measurements of all 39 metabolites were achieved within a 15min runtime. The method was evaluated with a Pseudomonas aeruginosa cell extract study (n=24) and a larger human urine study (n=308). Batch effects were observed in the urine study and an investigation of instrument and sample stability showed a wave-like pattern in the MS responses. Both the run order and inter-batch variation were successfully corrected by normalising to pooled urine quality control data. Thus, this method should be suitable for diverse biological matrices and for large as well as small sample sets.
Keywords	liquid chromatography ; metabolites ; cadaverine ; spermidine ; dipeptides ; spermine ; humans ; urine ; putrescine ; tandem mass spectrometry ; quantitative analysis ; amino acids ; quality control ; Pseudomonas aeruginosa ; agmatine
Language	English
Document type	Article
ISSN	1570-0232
Database	AGRIS - International Information System for the Agricultural Sciences and Technology

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED