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  1. Article: Immunoinformatics and reverse vaccinology approach in designing a novel highly immunogenic multivalent peptide-based vaccine against the human monkeypox virus.

    Choudhury, Abhigyan / Chandra, Anshuman / Dawoud, Turki M / Nafidi, Hiba-Allah / Singh, Nagendra / Bourhia, Mohammed

    Frontiers in molecular biosciences

    2023  Volume 10, Page(s) 1295817

    Abstract: Background: ...

    Abstract Background:
    Language English
    Publishing date 2023-11-22
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2814330-9
    ISSN 2296-889X
    ISSN 2296-889X
    DOI 10.3389/fmolb.2023.1295817
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  2. Article ; Online: Integrative Taxonomy of Novel

    Monkai, Jutamart / Hongsanan, Sinang / Bhat, Darbhe J / Dawoud, Turki M / Lumyong, Saisamorn

    Journal of fungi (Basel, Switzerland)

    2023  Volume 9, Issue 6

    Abstract: During our investigations of the microfungi on medicinal plants in Thailand, five isolates ... ...

    Abstract During our investigations of the microfungi on medicinal plants in Thailand, five isolates of
    Language English
    Publishing date 2023-05-24
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2784229-0
    ISSN 2309-608X ; 2309-608X
    ISSN (online) 2309-608X
    ISSN 2309-608X
    DOI 10.3390/jof9060603
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  3. Article ; Online: Effect of glyphosate on the growth and survival of rhizobia isolated from root nodules of grass pea (Lathyrus sativus L.).

    Asrat, Atrsaw / Sitotaw, Baye / Dawoud, Turki M / Nafidi, Hiba-Allah / Bourhia, Mohammed / Mekuriaw, Animut / Wondmie, Gezahign Fentahun

    Scientific reports

    2023  Volume 13, Issue 1, Page(s) 21535

    Abstract: Grass pea (L. sativus L.) is a widely cultivated crop worldwide, forming a symbiotic relationship with nitrogen-fixing rhizobia. Glyphosate is commonly used by farmers for weed control during agricultural processes. However, the application of this ... ...

    Abstract Grass pea (L. sativus L.) is a widely cultivated crop worldwide, forming a symbiotic relationship with nitrogen-fixing rhizobia. Glyphosate is commonly used by farmers for weed control during agricultural processes. However, the application of this chemical herbicide negatively impacts soil fertility by affecting the nitrogen-fixing rhizobia. This study aimed to assess the effects of glyphosate on rhizobia isolated from healthy and robust Grass pea plants. Specifically, Grass pea plants exhibiting vigorous growth and a healthy appearance were intentionally selected to isolate rhizobia from their root nodules. The isolated rhizobia were then characterized based on their morphological features, biochemical properties, and resistance to abiotic traits. Rhizobial isolates from grass peas exhibited Gram-negative, rod-shaped morphology, milky colony color, and variable colony sizes. Additionally, the majority displayed smooth colony surfaces on yeast extract mannitol agar medium. Based on morphological and biochemical characteristics, the isolates could be grouped under the genus Rhizobium. Optimum growth conditions for these isolates were observed at temperatures between 28 and 38 °C, pH levels ranging from 5 to 8, and salt (NaCl) concentrations of 0.5% and 1%. At a concentration of 20 mL L
    MeSH term(s) Rhizobium/physiology ; Pisum sativum ; Lathyrus ; Symbiosis ; Nitrogen ; Root Nodules, Plant
    Chemical Substances Nitrogen (N762921K75)
    Language English
    Publishing date 2023-12-06
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-48424-7
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  4. Article: Prevalence of Multidrug-Resistant Bacteria in Healthcare-Associated Bloodstream Infections at Hospitals in Riyadh, Saudi Arabia.

    Banawas, Saeed S / Alobaidi, Ahmed S / Dawoud, Turki M / AlDehaimi, Abdullah / Alsubaie, Faisal M / Abdel-Hadi, Ahmed / Manikandan, Palanisamy

    Pathogens (Basel, Switzerland)

    2023  Volume 12, Issue 9

    Abstract: Bloodstream infection (BSI) prevalence in hospitalized patients has increased owing to the spread of antibiotic-resistant pathogens; moreover, antimicrobial resistance in bacteria is a global problem. Here, BSIs are investigated in several patients at a ... ...

    Abstract Bloodstream infection (BSI) prevalence in hospitalized patients has increased owing to the spread of antibiotic-resistant pathogens; moreover, antimicrobial resistance in bacteria is a global problem. Here, BSIs are investigated in several patients at a hospital in Saudi Arabia, and the resistance of bacterial isolates to widely used drugs is determined. Throughout 2020, bacteria isolated from patients were identified and subjected to antibiotic susceptibility testing. In total, 1125 bacterial isolates were obtained from 1039 patients; among them, gram-positive bacteria were significantly more abundant than gram-negative bacteria. The most prevalent bacteria were
    Language English
    Publishing date 2023-08-23
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2695572-6
    ISSN 2076-0817
    ISSN 2076-0817
    DOI 10.3390/pathogens12091075
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  5. Article: Occurrence and characterization of β-lactamase-producing bacteria in biomedical wastewater and

    Mannan, Sultana Juhara / Akash, Shopnil / Jahin, Sumaya Afnan / Saqif, Ahnaf Tahmid / Begum, Kohinur / Yasmin, Mahmuda / Ahsan, Chowdhury Rafiqul / Sitotaw, Baye / Dawoud, Turki M / Nafidi, Hiba-Allah / Bourhia, Mohammed

    Frontiers in microbiology

    2024  Volume 14, Page(s) 1292597

    Abstract: Wastewater discharged from hospitals is a recognized contributor to the dissemination of antibiotic-resistant bacteria and their associated genetic traits into the environment. This study focused on the analysis of β-lactamase-producing pathogenic ... ...

    Abstract Wastewater discharged from hospitals is a recognized contributor to the dissemination of antibiotic-resistant bacteria and their associated genetic traits into the environment. This study focused on the analysis of β-lactamase-producing pathogenic bacteria within untreated biomedical wastewater originating from various hospitals in Dhaka City, Bangladesh, as well as
    Language English
    Publishing date 2024-01-08
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2023.1292597
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  6. Article ; Online: Utilization of microalgae for agricultural runoff remediation and sustainable biofuel production through an integrated biorefinery approach.

    Rana, Qurrat Ul Ain / Latif, Saira / Perveen, Saleha / Haq, Abdul / Ali, Sidra / Irfan, Muhammad / Gauttam, Rahul / Shah, Tawaf Ali / Dawoud, Turki M / Wondmie, Gezahign Fentahun / Bourhia, Mohammed / Badshah, Malik

    Bioresources and bioprocessing

    2024  Volume 11, Issue 1, Page(s) 8

    Abstract: Generally wastewater such agricultural runoff is considered a nuisance; however, it could be harnessed as a potential source of nutrients like nitrates and phosphates in integrated biorefinery context. In the current study, microalgae Chlorella sp. S5 ... ...

    Abstract Generally wastewater such agricultural runoff is considered a nuisance; however, it could be harnessed as a potential source of nutrients like nitrates and phosphates in integrated biorefinery context. In the current study, microalgae Chlorella sp. S5 was used for bioremediation of agricultural runoff and the leftover algal biomass was used as a potential source for production of biofuels in an integrated biorefinery context. The microalgae Chlorella sp. S5 was cultivated on Blue Green (BG 11) medium and a comprehensive optimization of different parameters including phosphates, nitrates, and pH was carried out to acquire maximum algal biomass enriched with high lipids content. Dry biomass was quantified using the solvent extraction technique, while the identification of nitrates and phosphates in agricultural runoff was carried out using commercial kits. The algal extracted lipids (oils) were employed in enzymatic trans-esterification for biodiesel production using whole-cell biomass of Bacillus subtilis Q4 MZ841642. The resultant fatty acid methyl esters (FAMEs) were analyzed using Fourier transform infrared (FTIR) spectroscopy and gas chromatography coupled with mass spectrometry (GC-MS). Subsequently, both the intact algal biomass and its lipid-depleted algal biomass were used for biogas production within a batch anaerobic digestion setup. Interestingly, Chlorella sp. S5 demonstrated a substantial reduction of 95% in nitrate and 91% in phosphate from agricultural runoff. The biodiesel derived from algal biomass exhibited a noteworthy total FAME content of 98.2%, meeting the quality standards set by American Society for Testing and Materials (ASTM) and European union (EU) standards. Furthermore, the biomethane yields obtained from whole biomass and lipid-depleted biomass were 330.34 NmL/g VS
    Language English
    Publishing date 2024-01-11
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2785482-6
    ISSN 2197-4365 ; 2197-4365
    ISSN (online) 2197-4365
    ISSN 2197-4365
    DOI 10.1186/s40643-023-00720-w
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  7. Article ; Online: Exploring ascomycete diversity in Yunnan II: Introducing three novel species in the suborder Massarineae (Dothideomycetes, Pleosporales) from fern and grasses.

    Phookamsak, Rungtiwa / Hongsanan, Sinang / Bhat, Darbhe Jayarama / Wanasinghe, Dhanushka N / Promputtha, Itthayakorn / Suwannarach, Nakarin / Kumla, Jaturong / Xie, Ning / Dawoud, Turki M / Mortimer, Peter E / Xu, Jianchu / Lumyong, Saisamorn

    MycoKeys

    2024  Volume 104, Page(s) 9–50

    Abstract: This article presents the results of an ongoing inventory of Ascomycota in Yunnan, China, carried out as part of the research project series "Exploring ascomycete diversity in Yunnan". From over 100 samples collected from diverse host substrates, ... ...

    Abstract This article presents the results of an ongoing inventory of Ascomycota in Yunnan, China, carried out as part of the research project series "Exploring ascomycete diversity in Yunnan". From over 100 samples collected from diverse host substrates, microfungi have been isolated, identified and are currently being documented. The primary objective of this research is to promote the discovery of novel taxa and explore the ascomycete diversity in the region, utilising a morphology-phylogeny approach. This article represents the second series of species descriptions for the project and introduces three undocumented species found in the families Bambusicolaceae, Dictyosporiaceae and Periconiaceae, belonging to the suborder Massarineae (Pleosporales, Dothideomycetes). These novel taxa exhibit typical morphological characteristics of
    Language English
    Publishing date 2024-04-16
    Publishing country Bulgaria
    Document type Journal Article
    ZDB-ID 2630648-7
    ISSN 1314-4049 ; 1314-4049
    ISSN (online) 1314-4049
    ISSN 1314-4049
    DOI 10.3897/mycokeys.104.112149
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  8. Article ; Online: Screening and optimization of extracellular pectinase produced by Bacillus thuringiensis SH7

    Umar Maria / Rehman Aneela / Khan Ibrar / Hayat Palwasha / Hayat Azam / Rehman Mujaddad Ur / Shah Tawaf Ali / Dawoud Turki M. / Hadrach Safaa / Bourhia Mohammed

    Open Chemistry, Vol 21, Iss 1, Pp H2281-

    2023  Volume 90

    Abstract: The objective of the current research was to identify and evaluate the possibility of production of pectinase, also known as pectin degrading enzymes, from indigenous bacterial strains. Qualitative screening of isolated bacterial strains showed that ... ...

    Abstract The objective of the current research was to identify and evaluate the possibility of production of pectinase, also known as pectin degrading enzymes, from indigenous bacterial strains. Qualitative screening of isolated bacterial strains showed that among 29 bacterial strains, 5 have maximum enzymatic activity. The highest pectinase producing strains were quantitatively analyzed for enzyme production. SH7 strain was found as highest pectinase producer (0.77 IU/mL) that was further analyzed to molecular level by amplification of 16s rRNA. It was found 100% similar with other reported strains of Bacillus thuringiensis. Medium optimization was performed to optimize fermentation conditions for maximum enzyme yield. An experimental design containing 12 experimental runs was designed by Plackett–Burman design (PBD). Maximum pectinase activity was obtained at 45°C after 24 h when the growth medium was supplemented with 2.5% nitrogen, 5.0% substrate, MgSO4 as metal ion, 1% inoculum size, and pH was adjusted to 6. Factorial regression analysis of the PBD design was performed and the overall design was also found significant in terms of R square value. In PBD, the most significant factors for production were temperature, pH, metal ion concentration, and nitrogen source. Central composite design (CCD) design consisting of 26 experimental runs was employed to optimize these four significant factors. The overall model summary showed maximum pectinase activity (19.2 IU/mL) at 37°C temperature, 0.08 NaCl, 1.7% nitrogen source, and pH 8.4. In CCD, NaCl, nitrogen source, and pH were also reported as significant factors by the Pareto chart, probability plots, and 3D interactions.
    Keywords plackett–burman design ; central composite design ; pectinases ; optimization ; fermentation ; Chemistry ; QD1-999
    Subject code 500
    Language English
    Publishing date 2023-07-01T00:00:00Z
    Publisher De Gruyter
    Document type Article ; Online
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  9. Article ; Online: Decolorization of acid blue 29, disperse red 1 and congo red by different indigenous fungal strains.

    Ameen, Fuad / Dawoud, Turki M / Alshehrei, Fatimah / Alsamhary, Khawla / Almansob, Abobakr

    Chemosphere

    2021  Volume 271, Page(s) 129532

    Abstract: Azo dyes are toxic and recalcitrant environmental pollutants in wastewater and soil in many industrial sites in Asia and Arabic countries. The aim of this study was to find fungal species useful in wastewater treatment and soil remediation efforts. We ... ...

    Abstract Azo dyes are toxic and recalcitrant environmental pollutants in wastewater and soil in many industrial sites in Asia and Arabic countries. The aim of this study was to find fungal species useful in wastewater treatment and soil remediation efforts. We assessed the ability of different indigenous Aspergillus strains (i.e. A. flavus, A. fumigatus, A. niger and A. terreus) to degrade the azo dyes Acid Blue 29 (AB29), Disperse Red 1 (DR1) and Congo Red (CR). The optimal conditions for dye decolorization by the above-mentioned strains appeared to be as follows: temperature range 30-35 °C, pH 7, glucose as the carbon source (10 g/L), ammonium sulphate as the nitrogen source (1.5 g/L) and 100 mg/L initial dye concentration. The Aspergillus strains decolorized all azo dyes more than 86%. The HPLC and GC-MS analyses confirmed that aniline (retention time 9.0 min), 3-nitroaniline (retention time 15.92 min), 4-nitroanline (retention time 17.81 min), N,N' diethyl-1,4-phenylendiamine (retention time 18.184 min), and benzidine (retention time 15.07 min) were formed as the intermediate metabolites of dye degradation. All Aspergillus strains decolorized 85% of the dyes in synthetic wastewater.
    MeSH term(s) Asia ; Azo Compounds ; Biodegradation, Environmental ; Coloring Agents ; Congo Red ; Naphthalenes ; Niger
    Chemical Substances Azo Compounds ; Coloring Agents ; Naphthalenes ; acid blue 29 ; Disperse Red 1 (2872-52-8) ; Congo Red (3U05FHG59S)
    Language English
    Publishing date 2021-01-05
    Publishing country England
    Document type Journal Article
    ZDB-ID 120089-6
    ISSN 1879-1298 ; 0045-6535 ; 0366-7111
    ISSN (online) 1879-1298
    ISSN 0045-6535 ; 0366-7111
    DOI 10.1016/j.chemosphere.2021.129532
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2540: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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  10. Article ; Online: Mycodegradation of diazinon pesticide utilizing fungal strains isolated from polluted soil.

    Abdel-Fattah Mostafa, Ashraf / Yassin, Mohamed Taha / Dawoud, Turki M / Al-Otibi, Fatimah O / Sayed, Shaban Rm

    Environmental research

    2022  Volume 212, Issue Pt C, Page(s) 113421

    Abstract: The current study aimed to isolate biodegradable soil fungi capable of metabolizing diazinon. The collected soil samples were investigated for diazinon pollution to detect the pesticide level in the polluted soil samples. Food poisoning techniques were ... ...

    Abstract The current study aimed to isolate biodegradable soil fungi capable of metabolizing diazinon. The collected soil samples were investigated for diazinon pollution to detect the pesticide level in the polluted soil samples. Food poisoning techniques were utilized to preliminary investigate the biodegradation efficiency of the isolated fungal strains to diazinon pesticide using solid and liquid medium and also to detect their tolerance to different concentrations. GC-MS analysis of control and treated flasks were achieved to determine the diazinon residues for confirmation of the biodegradation efficiency. The total diazinon residues in the collected soil samples was found to be 0.106 mg/kg. Out of thirteen fungal strains isolated form diazinon polluted soils, six strains were potentially active in diazinon biodegradation. Food poisoning technique showed that A. niger, B. antennata, F. graminearum, P. digitatum, R. stolonifer and T. viride strains recorded fungal growth diameters of 65.2 ± 0.18, 57.5 ± 0.41, 47.2 ± 0.36, 56.5 ± 0.27, 85.0 ± 0.01, 85.0 ± 0.06 mm respectively in the treated group which were non significantly different compared to that of control (P > 0.05), indicating the high efficiency of these strains in diazinon degradation compared to the other isolated strains. GC-MS analysis revealed that B. antennata was the most efficient strain in diazinon degradation recording 32.24 ± 0.15 ppm concentration after 10 days incubation. Linear regression analysis confirmed that B. antennata was the most effective biodegradable strain recording the highest diazinon dissipation (83.88%) with the lowest T
    MeSH term(s) Biodegradation, Environmental ; Diazinon/analysis ; Diazinon/chemistry ; Diazinon/metabolism ; Foodborne Diseases ; Fungi ; Pesticides/analysis ; Soil/chemistry ; Soil Microbiology ; Soil Pollutants/analysis
    Chemical Substances Pesticides ; Soil ; Soil Pollutants ; Diazinon (YUS1M1Q929)
    Language English
    Publishing date 2022-05-12
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 205699-9
    ISSN 1096-0953 ; 0013-9351
    ISSN (online) 1096-0953
    ISSN 0013-9351
    DOI 10.1016/j.envres.2022.113421
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    In stock of ZB MED Cologne/Königswinter

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