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  1. Article ; Online: A highly antigenic fragment within the zoonotic Cryptosporidium parvum Gp900 glycoprotein (Domain 3) is absent in human restricted Cryptosporidium species.

    Dayao, Denise Ann E / Jaskiewicz, Justyna J / Sheoran, Abhineet S / Widmer, Giovanni / Tzipori, Saul

    PloS one

    2023  Volume 18, Issue 8, Page(s) e0287997

    Abstract: We identified a fragment (Domain 3-D3) of the immunodominant sporozoite surface glycoprotein of the zoonotic parasite Cryptosporidium gp900, which is absent C. hominis and C. parvum anthroponosum. The fragment is highly antigenic and is able to ... ...

    Abstract We identified a fragment (Domain 3-D3) of the immunodominant sporozoite surface glycoprotein of the zoonotic parasite Cryptosporidium gp900, which is absent C. hominis and C. parvum anthroponosum. The fragment is highly antigenic and is able to effectively differentiate between zoonotic C. parvum and species/genotypes that infect preferentially humans. D3 detection provides a serological tool to determine whether the source of human cryptosporidiosis is of animal or human origin. We demonstrate this in experimentally challenged piglets, mice, rats, and alpaca. We speculate that the absence of this fragment from the C. hominis and C. parvum anthroponosum gp900 protein may play a key role in their host restriction.
    MeSH term(s) Humans ; Animals ; Mice ; Rats ; Swine ; Cryptosporidium parvum ; Cryptosporidium ; Cryptosporidiosis ; Glycoproteins ; Membrane Glycoproteins ; Camelids, New World ; Propionibacterium acnes
    Chemical Substances Glycoproteins ; Membrane Glycoproteins
    Language English
    Publishing date 2023-08-17
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0287997
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Scalable cryopreservation of infectious Cryptosporidium hominis oocysts by vitrification.

    Jaskiewicz, Justyna J / Dayao, Denise Ann E / Girouard, Donald / Sevenler, Derin / Widmer, Giovanni / Toner, Mehmet / Tzipori, Saul / Sandlin, Rebecca D

    PLoS pathogens

    2023  Volume 19, Issue 6, Page(s) e1011425

    Abstract: Cryptosporidium hominis is a serious cause of childhood diarrhea in developing countries. The development of therapeutics is impeded by major technical roadblocks including lack of cryopreservation and simple culturing methods. This impacts the ... ...

    Abstract Cryptosporidium hominis is a serious cause of childhood diarrhea in developing countries. The development of therapeutics is impeded by major technical roadblocks including lack of cryopreservation and simple culturing methods. This impacts the availability of optimized/standardized singular sources of infectious parasite oocysts for research and human challenge studies. The human C. hominis TU502 isolate is currently propagated in gnotobiotic piglets in only one laboratory, which limits access to oocysts. Streamlined cryopreservation could enable creation of a biobank to serve as an oocyst source for research and distribution to other investigators requiring C. hominis. Here, we report cryopreservation of C. hominis TU502 oocysts by vitrification using specially designed specimen containers scaled to 100 μL volume. Thawed oocysts exhibit ~70% viability with robust excystation and 100% infection rate in gnotobiotic piglets. The availability of optimized/standardized sources of oocysts may streamline drug and vaccine evaluation by enabling wider access to biological specimens.
    MeSH term(s) Animals ; Humans ; Swine ; Cryptosporidium ; Cryptosporidiosis/parasitology ; Vitrification ; Oocysts ; Cryopreservation ; Cryptosporidium parvum
    Language English
    Publishing date 2023-06-08
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, Non-U.S. Gov't
    ZDB-ID 2205412-1
    ISSN 1553-7374 ; 1553-7374
    ISSN (online) 1553-7374
    ISSN 1553-7374
    DOI 10.1371/journal.ppat.1011425
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Antimicrobial resistance in bacteria associated with porcine respiratory disease in Australia.

    Dayao, Denise Ann E / Gibson, Justine S / Blackall, Patrick J / Turni, Conny

    Veterinary microbiology

    2014  Volume 171, Issue 1-2, Page(s) 232–235

    Abstract: The porcine respiratory disease complex greatly affects the health and production of pigs. While antimicrobial agents are used to treat the respiratory infections caused by bacterial pathogens, there is no current information on antimicrobial resistance ... ...

    Abstract The porcine respiratory disease complex greatly affects the health and production of pigs. While antimicrobial agents are used to treat the respiratory infections caused by bacterial pathogens, there is no current information on antimicrobial resistance in Australian pig respiratory bacterial isolates. The aim of this study was to determine the antimicrobial resistance profiles, by determining the minimum inhibitory concentration of nine antimicrobial agents for 71 Actinobacillus pleuropneumoniae, 51 Pasteurella multocida and 18 Bordetella bronchiseptica cultured from Australian pigs. The majority of A. pleuropneumoniae isolates were resistant to erythromycin (89%) and tetracycline (75%). Resistance to ampicillin (8.5%), penicillin (8.5%) and tilmicosin (25%) was also identified. The P. multocida isolates exhibited resistance to co-trimoxazole (2%), florfenicol (2%), ampicillin (4%), penicillin (4%), erythromycin (14%) and tetracycline (28%). While all the B. bronchiseptica isolates showed resistance to beta-lactams (ampicillin, ceftiofur and penicillin), some were resistant to erythromycin (94%), florfenicol (6%), tilmicosin (22%) and tetracycline (39%). The incidence of multiple drug resistance (MDR) varied across the species - in B. bronchiseptica, 27.8% of resistant isolates showed MDR, while 9.1% of the resistant isolates in A. pleuropneumoniae, and 4.8% in P. multocida showed MDR. This study illustrated that Australian pig strains of bacterial respiratory pathogens exhibited low levels of resistance to antimicrobial agents commonly used in the pig industry.
    MeSH term(s) Animals ; Anti-Bacterial Agents/pharmacology ; Australia ; Drug Resistance, Bacterial ; Drug Resistance, Multiple ; Gram-Negative Bacteria/drug effects ; Gram-Negative Bacterial Infections/microbiology ; Gram-Negative Bacterial Infections/veterinary ; Microbial Sensitivity Tests ; Respiratory Tract Infections/microbiology ; Respiratory Tract Infections/veterinary ; Swine ; Swine Diseases/microbiology
    Chemical Substances Anti-Bacterial Agents
    Language English
    Publishing date 2014-06-25
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 753154-0
    ISSN 1873-2542 ; 0378-1135
    ISSN (online) 1873-2542
    ISSN 0378-1135
    DOI 10.1016/j.vetmic.2014.03.014
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2917: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Use of a proposed antimicrobial susceptibility testing method for Haemophilus parasuis.

    Dayao, Denise Ann E / Kienzle, Marco / Gibson, Justine S / Blackall, Patrick J / Turni, Conny

    Veterinary microbiology

    2014  Volume 172, Issue 3-4, Page(s) 586–589

    Abstract: The aim of this study was to examine the antimicrobial susceptibility of 97 Haemophilus parasuis cultured from Australian pigs. As there is no existing standard antimicrobial susceptibility technique available for H. parasuis, methods utilising the ... ...

    Abstract The aim of this study was to examine the antimicrobial susceptibility of 97 Haemophilus parasuis cultured from Australian pigs. As there is no existing standard antimicrobial susceptibility technique available for H. parasuis, methods utilising the supplemented media, BA/SN for disc diffusion and test medium broth (TMB) for a microdilution technique, were initially evaluated with the reference strains recommended by the Clinical and Laboratory Standards Institute. The results of the media evaluation suggested that BA/SN and TMB can be used as suitable media for susceptibility testing of H. parasuis. The proposed microdilution technique was then used with 97 H. parasuis isolates and nine antimicrobial agents. The study found that Australian isolates showed elevated minimum inhibitory concentrations (MICs) for ampicillin (1%), penicillin (2%), erythromycin (7%), tulathromycin (9%), tilmicosin (22%), tetracycline (31%) and trimethoprim-sulfamethoxazole (40%). This study has described potential antimicrobial susceptibility methods for H. parasuis and has detected a low percentage of Australian H. parasuis isolates with elevated antimicrobial MICs.
    MeSH term(s) Animals ; Anti-Bacterial Agents/pharmacology ; Bacteriological Techniques/veterinary ; Drug Resistance, Bacterial ; Haemophilus parasuis/isolation & purification ; Microbial Sensitivity Tests/standards
    Chemical Substances Anti-Bacterial Agents
    Language English
    Publishing date 2014-08-27
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 753154-0
    ISSN 1873-2542 ; 0378-1135
    ISSN (online) 1873-2542
    ISSN 0378-1135
    DOI 10.1016/j.vetmic.2014.06.010
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2917: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  5. Article ; Online: Variation in the Antimicrobial Susceptibility of Actinobacillus pleuropneumoniae Isolates in a Pig, Within a Batch of Pigs, and Among Batches of Pigs from One Farm.

    Dayao, Denise Ann E / Dawson, Susan / Kienzle, Marco Jean-Paul / Gibson, Justine S / Blackall, Patrick J / Turni, Conny

    Microbial drug resistance (Larchmont, N.Y.)

    2015  Volume 21, Issue 4, Page(s) 491–496

    Abstract: Antimicrobial resistance in bacterial porcine respiratory pathogens has been shown to exist in many countries. However, little is known about the variability in antimicrobial susceptibility within a population of a single bacterial respiratory pathogen ... ...

    Abstract Antimicrobial resistance in bacterial porcine respiratory pathogens has been shown to exist in many countries. However, little is known about the variability in antimicrobial susceptibility within a population of a single bacterial respiratory pathogen on a pig farm. This study examined the antimicrobial susceptibility of Actinobacillus pleuropneumoniae using multiple isolates within a pig and across the pigs in three different slaughter batches. Initially, the isolates from the three batches were identified, serotyped, and subsample genotyped. All the 367 isolates were identified as A. pleuropneumoniae serovar 1, and only a single genetic profile was detected in the 74 examined isolates. The susceptibility of the 367 isolates of A. pleuropneumoniae to ampicillin, tetracycline and tilmicosin was determined by a disc diffusion technique. For tilmicosin, the three batches were found to consist of a mix of susceptible and resistant isolates. The zone diameters of the three antimicrobials varied considerably among isolates in the second sampling. In addition, the second sampling provided statistically significant evidence of bimodal populations in terms of zone diameters for both tilmicosin and ampicillin. The results support the hypothesis that the antimicrobial susceptibility of one population of a porcine respiratory pathogen can vary within a batch of pigs on a farm.
    MeSH term(s) Actinobacillus Infections/microbiology ; Actinobacillus Infections/veterinary ; Actinobacillus pleuropneumoniae/drug effects ; Animals ; Anti-Bacterial Agents/pharmacology ; Drug Resistance, Bacterial ; Genotype ; Lung/microbiology ; Microbial Sensitivity Tests ; Pleuropneumonia/microbiology ; Pleuropneumonia/veterinary ; Swine ; Swine Diseases/microbiology
    Chemical Substances Anti-Bacterial Agents
    Language English
    Publishing date 2015-08
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1290490-9
    ISSN 1931-8448 ; 1076-6294
    ISSN (online) 1931-8448
    ISSN 1076-6294
    DOI 10.1089/mdr.2014.0265
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4435: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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