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  1. AU="Daymé Gonzalez Rodriguez"
  2. AU="Lou, Shuyi"
  3. AU="Figueiredo, Rodrigo S"
  4. AU=Fleet James C
  5. AU="Brohawn, David G"
  6. AU="Cho, Chun-Chieh"
  7. AU="van Raalte, Daniël H"
  8. AU="Zargarian, Loussiné"
  9. AU=Hascalovici Jacob
  10. AU="Spagnolo, Jennifer B"
  11. AU="Anderloni, Giulia"
  12. AU="Ahmad, Shoaib"
  13. AU="Du, Roujia"
  14. AU="Colmenero-Repiso, Ana"
  15. AU="Alvarez-Carbonell, David"
  16. AU="Phelippeau, Michael"
  17. AU="Lunghi, Laura"
  18. AU=Giersiepen Klaus
  19. AU="Drobyshev, Sergey"
  20. AU="Timme, Kathleen H"
  21. AU=Sfriso Paolo
  22. AU="Kim, John S"
  23. AU=Farkash Evan A AU=Farkash Evan A
  24. AU="Xia, Xueqian"

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  1. Artikel ; Online: scRNA-Seq of Cultured Human Amniotic Fluid from Fetuses with Spina Bifida Reveals the Origin and Heterogeneity of the Cellular Content

    Athanasia Dasargyri / Daymé González Rodríguez / Hubert Rehrauer / Ernst Reichmann / Thomas Biedermann / Ueli Moehrlen

    Cells, Vol 12, Iss 1577, p

    2023  Band 1577

    Abstract: Amniotic fluid has been proposed as an easily available source of cells for numerous applications in regenerative medicine and tissue engineering. The use of amniotic fluid cells in biomedical applications necessitates their unequivocal characterization; ...

    Abstract Amniotic fluid has been proposed as an easily available source of cells for numerous applications in regenerative medicine and tissue engineering. The use of amniotic fluid cells in biomedical applications necessitates their unequivocal characterization; however, the exact cellular composition of amniotic fluid and the precise tissue origins of these cells remain largely unclear. Using cells cultured from the human amniotic fluid of fetuses with spina bifida aperta and of a healthy fetus, we performed single-cell RNA sequencing to characterize the tissue origin and marker expression of cultured amniotic fluid cells at the single-cell level. Our analysis revealed nine different cell types of stromal, epithelial and immune cell phenotypes, and from various fetal tissue origins, demonstrating the heterogeneity of the cultured amniotic fluid cell population at a single-cell resolution. It also identified cell types of neural origin in amniotic fluid from fetuses with spina bifida aperta. Our data provide a comprehensive list of markers for the characterization of the various progenitor and terminally differentiated cell types in cultured amniotic fluid. This study highlights the relevance of single-cell analysis approaches for the characterization of amniotic fluid cells in order to harness their full potential in biomedical research and clinical applications.
    Schlagwörter amniotic fluid cells ; single-cell RNA sequencing ; spina bifida ; amniotic fluid stem cells ; Biology (General) ; QH301-705.5
    Thema/Rubrik (Code) 532 ; 610
    Sprache Englisch
    Erscheinungsdatum 2023-06-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  2. Artikel ; Online: Intrinsic TGF-β signaling attenuates proximal tubule mitochondrial injury and inflammation in chronic kidney disease

    Merve Kayhan / Judith Vouillamoz / Daymé Gonzalez Rodriguez / Milica Bugarski / Yasutaka Mitamura / Julia Gschwend / Christoph Schneider / Andrew Hall / David Legouis / Cezmi A. Akdis / Leary Peter / Hubert Rehrauer / Leslie Gewin / Roland H. Wenger / Stellor Nlandu Khodo

    Nature Communications, Vol 14, Iss 1, Pp 1-

    2023  Band 15

    Abstract: Abstract Excessive TGF-β signaling and mitochondrial dysfunction fuel chronic kidney disease (CKD) progression. However, inhibiting TGF-β failed to impede CKD in humans. The proximal tubule (PT), the most vulnerable renal segment, is packed with giant ... ...

    Abstract Abstract Excessive TGF-β signaling and mitochondrial dysfunction fuel chronic kidney disease (CKD) progression. However, inhibiting TGF-β failed to impede CKD in humans. The proximal tubule (PT), the most vulnerable renal segment, is packed with giant mitochondria and injured PT is pivotal in CKD progression. How TGF-β signaling affects PT mitochondria in CKD remained unknown. Here, we combine spatial transcriptomics and bulk RNAseq with biochemical analyses to depict the role of TGF-β signaling on PT mitochondrial homeostasis and tubulo-interstitial interactions in CKD. Male mice carrying specific deletion of Tgfbr2 in the PT have increased mitochondrial injury and exacerbated Th1 immune response in the aristolochic acid model of CKD, partly, through impaired complex I expression and mitochondrial quality control associated with a metabolic rewiring toward aerobic glycolysis in the PT cells. Injured S3T2 PT cells are identified as the main mediators of the maladaptive macrophage/dendritic cell activation in the absence of Tgfbr2. snRNAseq database analyses confirm decreased TGF-β receptors and a metabolic deregulation in the PT of CKD patients. This study describes the role of TGF-β signaling in PT mitochondrial homeostasis and inflammation in CKD, suggesting potential therapeutic targets that might be used to mitigate CKD progression.
    Schlagwörter Science ; Q
    Thema/Rubrik (Code) 570
    Sprache Englisch
    Erscheinungsdatum 2023-06-01T00:00:00Z
    Verlag Nature Portfolio
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  3. Artikel ; Online: Circulating miRNAs as diagnostic biomarkers for adolescent idiopathic scoliosis

    José Luis García-Giménez / Pedro Antonio Rubio-Belmar / Lorena Peiró-Chova / David Hervás / Daymé González-Rodríguez / José Santiago Ibañez-Cabellos / Paloma Bas-Hermida / Salvador Mena-Mollá / Eva María García-López / Federico V. Pallardó / Teresa Bas

    Scientific Reports, Vol 8, Iss 1, Pp 1-

    2018  Band 12

    Abstract: Abstract The aetiology of adolescent idiopathic scoliosis (AIS) has been linked to many factors, such as asymmetric growth, neuromuscular condition, bone strength and genetic background. Recently, epigenetic factors have been proposed as contributors of ... ...

    Abstract Abstract The aetiology of adolescent idiopathic scoliosis (AIS) has been linked to many factors, such as asymmetric growth, neuromuscular condition, bone strength and genetic background. Recently, epigenetic factors have been proposed as contributors of AIS physiopathology, but information about the molecular mechanisms and pathways involved is scarce. Regarding epigenetic factors, microRNAs (miRNAs) are molecules that contribute to gene expression modulation by regulating important cellular pathways. We herein used Next-Generation Sequencing to discover a series of circulating miRNAs detected in the blood samples of AIS patients, which yielded a unique miRNA biomarker signature that diagnoses AIS with high sensitivity and specificity. We propose that these miRNAs participate in the epigenetic control of signalling pathways by regulating osteoblast and osteoclast differentiation, thus modulating the genetic background of AIS patients. Our study yielded two relevant results: 1) evidence for the deregulated miRNAs that participate in osteoblast/osteoclast differentiation mechanisms in AIS; 2) this miRNA-signature can be potentially used as a clinical tool for molecular AIS diagnosis. Using miRNAs as biomarkers for AIS diagnostics is especially relevant since miRNAs can serve for early diagnoses and for evaluating the positive effects of applied therapies to therefore reduce the need of high-risk surgical interventions.
    Schlagwörter Medicine ; R ; Science ; Q
    Thema/Rubrik (Code) 500
    Sprache Englisch
    Erscheinungsdatum 2018-02-01T00:00:00Z
    Verlag Nature Publishing Group
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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