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  1. Article ; Online: Charge Mapping of

    Rabinowitz, Jake / Hartel, Andreas J W / Dayton, Hannah / Fabbri, Jason D / Jo, Jeanyoung / Dietrich, Lars E P / Shepard, Kenneth L

    Analytical chemistry

    2023  Volume 95, Issue 12, Page(s) 5285–5292

    Abstract: Scanning ion conductance microscopy (SICM) is a topographic imaging technique capable of probing biological samples in electrolyte conditions. SICM enhancements have enabled surface charge detection based on voltage-dependent signals. Here, we show how ... ...

    Abstract Scanning ion conductance microscopy (SICM) is a topographic imaging technique capable of probing biological samples in electrolyte conditions. SICM enhancements have enabled surface charge detection based on voltage-dependent signals. Here, we show how the hopping mode SICM method (HP-SICM) can be used for rapid and minimally invasive surface charge mapping. We validate our method using
    MeSH term(s) Microscopy/methods ; Pseudomonas aeruginosa ; Radionuclide Imaging ; Ions ; Movement
    Chemical Substances Ions
    Language English
    Publishing date 2023-03-15
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, N.I.H., Extramural
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.2c05303
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4033: Show issues Location:
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  2. Article ; Online: Charge Mapping of Pseudomonas aeruginosa Using a Hopping Mode Scanning Ion Conductance Microscopy Technique

    Rabinowitz, Jake / Hartel, Andreas J. W. / Dayton, Hannah / Fabbri, Jason D. / Jo, Jeanyoung / Dietrich, Lars E. P. / Shepard, Kenneth L.

    Analytical Chemistry. 2023 Mar. 15, v. 95, no. 12 p.5285-5292

    2023  

    Abstract: Scanning ion conductance microscopy (SICM) is a topographic imaging technique capable of probing biological samples in electrolyte conditions. SICM enhancements have enabled surface charge detection based on voltage-dependent signals. Here, we show how ... ...

    Abstract Scanning ion conductance microscopy (SICM) is a topographic imaging technique capable of probing biological samples in electrolyte conditions. SICM enhancements have enabled surface charge detection based on voltage-dependent signals. Here, we show how the hopping mode SICM method (HP-SICM) can be used for rapid and minimally invasive surface charge mapping. We validate our method usingPseudomonas aeruginosaPA14 (PA) cells and observe a surface charge density of σPA = −2.0 ± 0.45 mC/m² that is homogeneous within the ∼80 nm lateral scan resolution. This biological surface charge is detected from at least 1.7 μm above the membrane (395× the Debye length), and the long-range charge detection is attributed to electroosmotic amplification. We show that imaging with a nanobubble-plugged probe reduces perturbation of the underlying sample. We extend the technique to PA biofilms and observe a charge density exceeding −20 mC/m². We use a solid-state calibration to quantify surface charge density and show that HP-SICM cannot be quantitatively described by a steady-state finite element model. This work contributes to the body of scanning probe methods that can uniquely contribute to microbiology and cellular biology.
    Keywords Pseudomonas aeruginosa ; analytical chemistry ; biofilm ; cell biology ; electrolytes ; electroosmosis ; finite element analysis ; microbiology ; microscopy ; models ; topography
    Language English
    Dates of publication 2023-0315
    Size p. 5285-5292.
    Publishing place American Chemical Society
    Document type Article ; Online
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.2c05303
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

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  3. Article ; Online: Cellular arrangement impacts metabolic activity and antibiotic tolerance in Pseudomonas aeruginosa biofilms.

    Dayton, Hannah / Kiss, Julie / Wei, Mian / Chauhan, Shradha / LaMarre, Emily / Cornell, William Cole / Morgan, Chase J / Janakiraman, Anuradha / Min, Wei / Tomer, Raju / Price-Whelan, Alexa / Nirody, Jasmine A / Dietrich, Lars E P

    PLoS biology

    2024  Volume 22, Issue 2, Page(s) e3002205

    Abstract: Cells must access resources to survive, and the anatomy of multicellular structures influences this access. In diverse multicellular eukaryotes, resources are provided by internal conduits that allow substances to travel more readily through tissue than ... ...

    Abstract Cells must access resources to survive, and the anatomy of multicellular structures influences this access. In diverse multicellular eukaryotes, resources are provided by internal conduits that allow substances to travel more readily through tissue than they would via diffusion. Microbes growing in multicellular structures, called biofilms, are also affected by differential access to resources and we hypothesized that this is influenced by the physical arrangement of the cells. In this study, we examined the microanatomy of biofilms formed by the pathogenic bacterium Pseudomonas aeruginosa and discovered that clonal cells form striations that are packed lengthwise across most of a mature biofilm's depth. We identified mutants, including those defective in pilus function and in O-antigen attachment, that show alterations to this lengthwise packing phenotype. Consistent with the notion that cellular arrangement affects access to resources within the biofilm, we found that while the wild type shows even distribution of tested substrates across depth, the mutants show accumulation of substrates at the biofilm boundaries. Furthermore, we found that altered cellular arrangement within biofilms affects the localization of metabolic activity, the survival of resident cells, and the susceptibility of subpopulations to antibiotic treatment. Our observations provide insight into cellular features that determine biofilm microanatomy, with consequences for physiological differentiation and drug sensitivity.
    MeSH term(s) Humans ; Anti-Bacterial Agents/pharmacology ; Pseudomonas aeruginosa/metabolism ; Biofilms ; Pseudomonas Infections/microbiology ; Fimbriae, Bacterial
    Chemical Substances Anti-Bacterial Agents
    Language English
    Publishing date 2024-02-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2126776-5
    ISSN 1545-7885 ; 1544-9173
    ISSN (online) 1545-7885
    ISSN 1544-9173
    DOI 10.1371/journal.pbio.3002205
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6193: Show issues Location:
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  4. Article: Scalable projected Light Sheet Microscopy for high-resolution imaging of living and cleared samples.

    Chen, Yannan / Chauhan, Shradha / Gong, Cheng / Dayton, Hannah / Xu, Cong / De La Cruz, Estanislao Daniel / Datta, Malika S / Leong, Kam W / Dietrich, Lars E P / Tomer, Raju

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Light sheet fluorescence microscopy (LSFM) is a widely used imaging technique for living and large cleared samples. However, high-performance LSFM systems are often prohibitively expensive and not easily scalable for high-throughput applications. Here, ... ...

    Abstract Light sheet fluorescence microscopy (LSFM) is a widely used imaging technique for living and large cleared samples. However, high-performance LSFM systems are often prohibitively expensive and not easily scalable for high-throughput applications. Here, we introduce a cost-effective, scalable, and versatile high-resolution imaging framework, called projected Light Sheet Microscopy (pLSM), which repurposes readily available off-the-shelf consumer-grade components and an over-the-network control architecture to achieve high-resolution imaging of living and cleared samples. We extensively characterize the pLSM framework and showcase its capabilities through high-resolution, multi-color imaging and quantitative analysis of mouse and post-mortem human brain samples cleared using various techniques. Moreover, we show the applicability of pLSM for high-throughput molecular phenotyping of human induced pluripotent cells (iPSC)-derived brain and vessel organoids. Additionally, we utilized pLSM for comprehensive live imaging of bacterial pellicle biofilms at the air-liquid interface, uncovering their intricate layered architecture and diverse cellular dynamics across different depths. Overall, the pLSM framework has the potential to further democratize LSFM by making high-resolution light sheet microscopy more accessible and scalable.
    Language English
    Publishing date 2023-07-18
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.05.31.543173
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Cell arrangement impacts metabolic activity and antibiotic tolerance in

    Dayton, Hannah / Kiss, Julie / Wei, Mian / Chauhan, Shradha / LaMarre, Emily / Cornell, William Cole / Morgan, Chase J / Janakiraman, Anuradha / Min, Wei / Tomer, Raju / Price-Whelan, Alexa / Nirody, Jasmine A / Dietrich, Lars E P

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Cells must access resources to survive, and the anatomy of multicellular structures influences this access. In diverse multicellular eukaryotes, resources are provided by internal conduits that allow substances to travel more readily through tissue than ... ...

    Abstract Cells must access resources to survive, and the anatomy of multicellular structures influences this access. In diverse multicellular eukaryotes, resources are provided by internal conduits that allow substances to travel more readily through tissue than they would via diffusion. Microbes growing in multicellular structures, called biofilms, are also affected by differential access to resources and we hypothesized that this is influenced by the physical arrangement of the cells. In this study, we examined the microanatomy of biofilms formed by the pathogenic bacterium
    Language English
    Publishing date 2023-08-28
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.06.20.545666
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Spatial heterogeneity in biofilm metabolism elicited by local control of phenazine methylation.

    Evans, Christopher R / Smiley, Marina K / Asahara Thio, Sean / Wei, Mian / Florek, Lindsey C / Dayton, Hannah / Price-Whelan, Alexa / Min, Wei / Dietrich, Lars E P

    Proceedings of the National Academy of Sciences of the United States of America

    2023  Volume 120, Issue 43, Page(s) e2313208120

    Abstract: Within biofilms, gradients of electron acceptors such as oxygen stimulate the formation of physiological subpopulations. This heterogeneity can enable cross-feeding and promote drug resilience, features of the multicellular lifestyle that make biofilm- ... ...

    Abstract Within biofilms, gradients of electron acceptors such as oxygen stimulate the formation of physiological subpopulations. This heterogeneity can enable cross-feeding and promote drug resilience, features of the multicellular lifestyle that make biofilm-based infections difficult to treat. The pathogenic bacterium
    MeSH term(s) Methylation ; Phenazines/pharmacology ; RNA/metabolism ; Biofilms ; Pseudomonas aeruginosa/metabolism ; Bacterial Proteins/metabolism
    Chemical Substances phenazine ; Phenazines ; RNA (63231-63-0) ; Bacterial Proteins
    Language English
    Publishing date 2023-10-17
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    DOI 10.1073/pnas.2313208120
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1148: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  7. Article ; Online: Drosophila

    Doupé, David P / Marshall, Owen J / Dayton, Hannah / Brand, Andrea H / Perrimon, Norbert

    Proceedings of the National Academy of Sciences of the United States of America

    2018  Volume 115, Issue 48, Page(s) 12218–12223

    Abstract: Epithelial homeostasis requires the precise balance of epithelial stem/progenitor proliferation and differentiation. While many signaling pathways that regulate epithelial stem cells have been identified, it is probable that other regulators remain ... ...

    Abstract Epithelial homeostasis requires the precise balance of epithelial stem/progenitor proliferation and differentiation. While many signaling pathways that regulate epithelial stem cells have been identified, it is probable that other regulators remain unidentified. Here, we use gene-expression profiling by targeted DamID to identify the stem/progenitor-specific transcription and signaling factors in the
    MeSH term(s) Animals ; Drosophila/cytology ; Drosophila/physiology ; Drosophila Proteins/metabolism ; Homeostasis ; Intestines/cytology ; Signal Transduction ; Stem Cell Niche ; Stem Cells/cytology ; Stem Cells/metabolism
    Chemical Substances Drosophila Proteins
    Language English
    Publishing date 2018-11-07
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    DOI 10.1073/pnas.1719169115
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1148: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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