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  1. AU="De, Yanyan"
  2. AU="Zheng-Yan, Gao"
  3. AU="Carvajal-Maldonado, Denisse"
  4. AU="Gallagher, Julia E"
  5. AU="Skelin, Ivan"
  6. AU="Pinarli, Faruk Güçlü"
  7. AU=Carmina E
  8. AU=Abi-Rafeh Jad
  9. AU="Jalil, Yorschua F"
  10. AU="Barber, M"
  11. AU="Ritt, Luiz Eduardo Fonteles"
  12. AU="Qiu, Jiajing"
  13. AU=Wang Heping
  14. AU="Miyazaki, Masashi"
  15. AU="R Kulkarni"
  16. AU="Braga, D."
  17. AU="Mwenda, Mulenga"
  18. AU="Li, Baohua"
  19. AU="Zhang, Nasen Jonathan"
  20. AU="Scotlandi, Katia"
  21. AU="Thomson, M A"
  22. AU=New Sophie E P
  23. AU="Fenrich, Craig A"
  24. AU="Staehelin, Cornelia"
  25. AU="Akhtar, Suraiya"
  26. AU="Georgel, Philippe"
  27. AU="Gruenewald, Leon D"
  28. AU="Charron, Morgane"
  29. AU="Leona S. Alizadeh" AU="Leona S. Alizadeh"
  30. AU="Soriano, Stéphane"
  31. AU="Lin, Pao-Yen"
  32. AU="Mudali, Gayathri"
  33. AU="McElveen, John T"
  34. AU="Kraimps, Jean-Louis"
  35. AU="Patel, Sheila K"
  36. AU="Zian, Zeineb"
  37. AU="Langley, Jonathan"
  38. AU="Bell, Thomas G."
  39. AU="Harris, Charles"
  40. AU="Lai, Renfa"
  41. AU="Sakane, Tatsuya"
  42. AU="Mirza, I."
  43. AU="Beatriz Amorim Beltrão"
  44. AU="Wildman, D"
  45. AU="Manghi, Manoel"
  46. AU="van Dinther, Maarten"
  47. AU="Adams, Ashley L"
  48. AU="Zhang, Er-Bin"
  49. AU="Diuk-Wasser, Maria A"
  50. AU="Chowdhury, Muhtamim"
  51. AU="Rivas, Manuel A"
  52. AU="Mangelis, Anastasios"
  53. AU="Simpson, Tina Y"
  54. AU="Li, Peirang"
  55. AU="Zhang, Zhao-Liang"
  56. AU="Perner, Sven"
  57. AU=Suwanwongse Kulachanya AU=Suwanwongse Kulachanya
  58. AU="Rose, Jacqueline"
  59. AU="E Lostis"

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  1. Artikel ; Online: HDGFRP3 interaction with 53BP1 promotes DNA double-strand break repair.

    Zhang, Zhen / Samsa, William E / De, Yanyan / Zhang, Fan / Reizes, Ofer / Almasan, Alexandru / Gong, Zihua

    Nucleic acids research

    2023  Band 51, Heft 5, Seite(n) 2238–2256

    Abstract: The 53BP1-dependent end-joining pathway plays a critical role in double-strand break (DSB) repair. However, the regulators of 53BP1 in chromatin remain incompletely characterized. In this study, we identified HDGFRP3 (hepatoma-derived growth factor ... ...

    Abstract The 53BP1-dependent end-joining pathway plays a critical role in double-strand break (DSB) repair. However, the regulators of 53BP1 in chromatin remain incompletely characterized. In this study, we identified HDGFRP3 (hepatoma-derived growth factor related protein 3) as a 53BP1-interacting protein. The HDGFRP3-53BP1 interaction is mediated by the PWWP domain of HDGFRP3 and the Tudor domain of 53BP1. Importantly, we observed that the HDGFRP3-53BP1 complex co-localizes with 53BP1 or γH2AX at sites of DSB and participates in the response to DNA damage repair. Loss of HDGFRP3 impairs classical non-homologous end-joining repair (NHEJ), curtails the accumulation of 53BP1 at DSB sites, and enhances DNA end-resection. Moreover, the HDGFRP3-53BP1 interaction is required for cNHEJ repair, 53BP1 recruitment at DSB sites, and inhibition of DNA end resection. In addition, loss of HDGFRP3 renders BRCA1-deficient cells resistant to PARP inhibitors by facilitating end-resection in BRCA1 deficient cells. We also found that the interaction of HDGFRP3 with methylated H4K20 was dramatically decreased; in contrast, the 53BP1-methylated H4K20 interaction was increased after ionizing radiation, which is likely regulated by protein phosphorylation and dephosphorylation. Taken together, our data reveal a dynamic 53BP1-methylated H4K20-HDGFRP3 complex that regulates 53BP1 recruitment at DSB sites, providing new insights into our understanding of the regulation of 53BP1-mediated DNA repair pathway.
    Mesh-Begriff(e) Humans ; BRCA1 Protein/genetics ; BRCA1 Protein/metabolism ; Cell Line ; DNA/genetics ; DNA/metabolism ; DNA Breaks, Double-Stranded ; DNA End-Joining Repair ; DNA Repair ; Tumor Suppressor p53-Binding Protein 1/genetics ; Tumor Suppressor p53-Binding Protein 1/metabolism
    Chemische Substanzen BRCA1 Protein ; DNA (9007-49-2) ; Tumor Suppressor p53-Binding Protein 1
    Sprache Englisch
    Erscheinungsdatum 2023-02-03
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 186809-3
    ISSN 1362-4962 ; 1362-4954 ; 0301-5610 ; 0305-1048
    ISSN (online) 1362-4962 ; 1362-4954
    ISSN 0301-5610 ; 0305-1048
    DOI 10.1093/nar/gkad073
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: Ortholog-based screening and identification of genes related to intracellular survival.

    Yang, Xiaowen / Wang, Jiawei / Bing, Guoxia / Bie, Pengfei / De, Yanyan / Lyu, Yanli / Wu, Qingmin

    Gene

    2018  Band 651, Seite(n) 134–142

    Abstract: Bioinformatics and comparative genomics analysis methods were used to predict unknown pathogen genes based on homology with identified or functionally clustered genes. In this study, the genes of common pathogens were analyzed to screen and identify ... ...

    Abstract Bioinformatics and comparative genomics analysis methods were used to predict unknown pathogen genes based on homology with identified or functionally clustered genes. In this study, the genes of common pathogens were analyzed to screen and identify genes associated with intracellular survival through sequence similarity, phylogenetic tree analysis and the λ-Red recombination system test method. The total 38,952 protein-coding genes of common pathogens were divided into 19,775 clusters. As demonstrated through a COG analysis, information storage and processing genes might play an important role intracellular survival. Only 19 clusters were present in facultative intracellular pathogens, and not all were present in extracellular pathogens. Construction of a phylogenetic tree selected 18 of these 19 clusters. Comparisons with the DEG database and previous research revealed that seven other clusters are considered essential gene clusters and that seven other clusters are associated with intracellular survival. Moreover, this study confirmed that clusters screened by orthologs with similar function could be replaced with an approved uvrY gene and its orthologs, and the results revealed that the usg gene is associated with intracellular survival. The study improves the current understanding of intracellular pathogens characteristics and allows further exploration of the intracellular survival-related gene modules in these pathogens.
    Mesh-Begriff(e) Animals ; Bacteria/genetics ; Bacterial Physiological Phenomena ; Bacterial Proteins/genetics ; Bacterial Proteins/physiology ; Cells/microbiology ; Cells, Cultured ; Genes, Bacterial ; Genes, Essential ; Host-Pathogen Interactions ; Mice ; Multigene Family ; Phylogeny
    Chemische Substanzen Bacterial Proteins
    Sprache Englisch
    Erscheinungsdatum 2018-04-20
    Erscheinungsland Netherlands
    Dokumenttyp Journal Article
    ZDB-ID 391792-7
    ISSN 1879-0038 ; 0378-1119
    ISSN (online) 1879-0038
    ISSN 0378-1119
    DOI 10.1016/j.gene.2018.01.059
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 1357: Hefte anzeigen Standort:
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  3. Artikel ; Online: Induction of potential protective immunity against enterotoxemia in calves by single or multiple recombinant Clostridium perfringens toxoids.

    Jiang, Zhigang / De, Yanyan / Chang, Jitao / Wang, Fang / Yu, Li

    Microbiology and immunology

    2014  Band 58, Heft 11, Seite(n) 621–627

    Abstract: Cattle enterotoxemia caused by Clostridium perfringens toxins is a noncontagious, sporadic, and fatal disease characterized by sudden death. Strategies for controlling and preventing cattle enterotoxemia are based on systematic vaccination of herds with ... ...

    Abstract Cattle enterotoxemia caused by Clostridium perfringens toxins is a noncontagious, sporadic, and fatal disease characterized by sudden death. Strategies for controlling and preventing cattle enterotoxemia are based on systematic vaccination of herds with toxoids. Because the process of producing conventional clostridial vaccines is dangerous, expensive, and time-consuming, the prospect of recombinant toxoid vaccines against diseases caused by C. perfringens toxins is promising. In this study, nontoxic recombinant toxoids derived from α-, β- and ε-toxins of C. perfringens, namely, rCPA247-370 , rCPB and rEtxHP, respectively, were expressed in Escherichia coli. High levels of specific IgG antibodies and neutralizing antibodies against the toxins were detected in sera from calves vaccinated with either a single recombinant toxoid or a mixed cocktail of all three recombinant toxoids, indicating the potential of these recombinant toxoids to provide calves with protective immunity against enterotoxemia caused by C. perfringens.
    Mesh-Begriff(e) Animals ; Antibodies, Bacterial/blood ; Antibodies, Neutralizing/blood ; Antitoxins/blood ; Cattle ; Cattle Diseases/prevention & control ; Clostridium Infections/prevention & control ; Clostridium Infections/veterinary ; Clostridium perfringens/genetics ; Clostridium perfringens/immunology ; Enterotoxemia/prevention & control ; Escherichia coli/genetics ; Female ; Gene Expression ; Immunoglobulin G/blood ; Recombinant Proteins/administration & dosage ; Recombinant Proteins/genetics ; Recombinant Proteins/immunology ; Recombinant Proteins/isolation & purification ; Toxoids/administration & dosage ; Toxoids/genetics ; Toxoids/immunology ; Toxoids/isolation & purification
    Chemische Substanzen Antibodies, Bacterial ; Antibodies, Neutralizing ; Antitoxins ; Clostridium perfringens toxoid ; Immunoglobulin G ; Recombinant Proteins ; Toxoids
    Sprache Englisch
    Erscheinungsdatum 2014-11
    Erscheinungsland Australia
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 224792-6
    ISSN 1348-0421 ; 0385-5600
    ISSN (online) 1348-0421
    ISSN 0385-5600
    DOI 10.1111/1348-0421.12198
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel ; Online: Genome-wide sequence transposon insertion sites and analyze the essential genes of Brucella melitensis.

    De, Yanyan / Dong, Congyue / Cao, Yanyang / Wang, Xiaolei / Yang, Xiaowen / Wang, Ning / Zhang, Cunrui / Wang, Zhen / Lyu, Yanli / Wu, Qingmin

    Microbial pathogenesis

    2017  Band 112, Seite(n) 97–102

    Abstract: A transposon mutant library of B. melitensis NI including 32,640 transposon mutants was established. By sequencing the transposon insertion sites, 10,832 mutants were successfully defined for their insertion sites. Analysis of the mutants with defined ... ...

    Abstract A transposon mutant library of B. melitensis NI including 32,640 transposon mutants was established. By sequencing the transposon insertion sites, 10,832 mutants were successfully defined for their insertion sites. Analysis of the mutants with defined transposon insertion sites (DTIS) indicated that the insertions were well spread through the two genomes. In addition, 948 genes with no detectable transposon insertions were taken as the candidate for identification of essential genes. In comparison with the Bacterial Database of Essential Genes and by using comparative genomics analysis, 183 potential essential genes of B. melitensis NI cultured in vitro were found and they were conserved in the common bacteria. This work was focused on screening of the essential genes of B. melitensis NI, which may provide a foundation for identification of the novel drug targets against brucellosis. Besides, the sequence-defined transposon library should serve as a resource for screening of different function genes of Brucella.
    Mesh-Begriff(e) Base Sequence ; Brucella melitensis/genetics ; Brucellosis/microbiology ; Chromosome Mapping ; Conjugation, Genetic ; DNA Transposable Elements/genetics ; Escherichia coli/genetics ; Gene Library ; Genes, Bacterial/genetics ; Genes, Essential/genetics ; Genome, Bacterial ; Genome-Wide Association Study ; Mutagenesis ; Mutagenesis, Insertional ; Mutation/genetics
    Chemische Substanzen DNA Transposable Elements
    Sprache Englisch
    Erscheinungsdatum 2017-11
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 632772-2
    ISSN 1096-1208 ; 0882-4010
    ISSN (online) 1096-1208
    ISSN 0882-4010
    DOI 10.1016/j.micpath.2017.09.005
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  5. Artikel: Development of an improved competitive ELISA based on a monoclonal antibody against lipopolysaccharide for the detection of bovine brucellosis

    Wang, Xiaolei / Wang, Yan / Ma, Limei / Zhang, Ran / De, Yanyan / Yang, Xiaowen / Wang, Chuanqing / Wu, Qingmin

    BMC veterinary research. 2015 Dec., v. 11, no. 1

    2015  

    Abstract: BACKGROUND: Brucellosis is the most common bacterial zoonosis, and serological tests are routinely used in brucellosis control and eradication programs. In order to improve the accuracy of serological diagnostic method used in bovine brucellosis ... ...

    Abstract BACKGROUND: Brucellosis is the most common bacterial zoonosis, and serological tests are routinely used in brucellosis control and eradication programs. In order to improve the accuracy of serological diagnostic method used in bovine brucellosis detection, this study developed an improved competitive ELISA with higher specificity and good sensitivity. RESULTS: This study prepared 12 monoclonal antibodies against smooth Brucella lipopolysaccharide. One monoclonal antibody 3 F9, presented C epitope specificity, was used to develop a competitive ELISA for the serological detection of bovine brucellosis. The competitive ELISA, a commercial competitive ELISA kit, the rose-bengal plate agglutination test, and a microplate agglutination test were all used in the detection of 6 hyperimmune antisera against other commonly cross-reacted bacterial pathogens and 110 clinical bovine serum samples. The results of the test comparisons indicated that the competitive ELISA had higher specificity than the commercial competitive ELISA kit and RBT, and comparable sensitivity with the commercial ELISA kit. CONCLUSIONS: This study provided a valuable detection tool with high specificity and good sensitivity, which prevent the wrong-culling of bovines in the eradication campaigns of bovine brucellosis.
    Schlagwörter Brucella ; agglutination tests ; antiserum ; blood serum ; bovine brucellosis ; cattle ; diagnostic techniques ; enzyme-linked immunosorbent assay ; epitopes ; lipopolysaccharides ; monoclonal antibodies ; pathogens ; zoonoses
    Sprache Englisch
    Erscheinungsverlauf 2015-12
    Umfang p. 436.
    Erscheinungsort Springer-Verlag
    Dokumenttyp Artikel
    ISSN 1746-6148
    DOI 10.1186/s12917-015-0436-3
    Datenquelle NAL Katalog (AGRICOLA)

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  6. Artikel: Genome-wide sequence transposon insertion sites and analyze the essential genes of Brucella melitensis

    De, Yanyan / Congyue Dong / Cunrui Zhang / Ning Wang / Qingmin Wu / Xiaolei Wang / Xiaowen Yang / Yanli Lyu / Yanyang Cao / Zhen Wang

    Microbial pathogenesis. 2017 Nov., v. 112

    2017  

    Abstract: A transposon mutant library of B. melitensis NI including 32,640 transposon mutants was established. By sequencing the transposon insertion sites, 10,832 mutants were successfully defined for their insertion sites. Analysis of the mutants with defined ... ...

    Abstract A transposon mutant library of B. melitensis NI including 32,640 transposon mutants was established. By sequencing the transposon insertion sites, 10,832 mutants were successfully defined for their insertion sites. Analysis of the mutants with defined transposon insertion sites (DTIS) indicated that the insertions were well spread through the two genomes. In addition, 948 genes with no detectable transposon insertions were taken as the candidate for identification of essential genes. In comparison with the Bacterial Database of Essential Genes and by using comparative genomics analysis, 183 potential essential genes of B. melitensis NI cultured in vitro were found and they were conserved in the common bacteria. This work was focused on screening of the essential genes of B. melitensis NI, which may provide a foundation for identification of the novel drug targets against brucellosis. Besides, the sequence-defined transposon library should serve as a resource for screening of different function genes of Brucella.
    Schlagwörter bacteria ; Brucella melitensis ; brucellosis ; databases ; drugs ; essential genes ; genomics ; mutants ; screening ; transposons
    Sprache Englisch
    Erscheinungsverlauf 2017-11
    Umfang p. 97-102.
    Erscheinungsort Elsevier Ltd
    Dokumenttyp Artikel
    ZDB-ID 632772-2
    ISSN 1096-1208 ; 0882-4010
    ISSN (online) 1096-1208
    ISSN 0882-4010
    DOI 10.1016/j.micpath.2017.09.005
    Datenquelle NAL Katalog (AGRICOLA)

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  7. Artikel: BASI74, a Virulence-Related sRNA in

    Dong, Hao / Peng, Xiaowei / Liu, Yufu / Wu, Tonglei / Wang, Xiaolei / De, Yanyan / Han, Tao / Yuan, Lin / Ding, Jiabo / Wang, Chuanbin / Wu, Qingmin

    Frontiers in microbiology

    2018  Band 9, Seite(n) 2173

    Abstract: ... ...

    Abstract Brucella
    Sprache Englisch
    Erscheinungsdatum 2018-09-13
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2018.02173
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  8. Artikel ; Online: Development of an improved competitive ELISA based on a monoclonal antibody against lipopolysaccharide for the detection of bovine brucellosis.

    Wang, Xiaolei / Wang, Yan / Ma, Limei / Zhang, Ran / De, Yanyan / Yang, Xiaowen / Wang, Chuanqing / Wu, Qingmin

    BMC veterinary research

    2015  Band 11, Seite(n) 118

    Abstract: Background: Brucellosis is the most common bacterial zoonosis, and serological tests are routinely used in brucellosis control and eradication programs. In order to improve the accuracy of serological diagnostic method used in bovine brucellosis ... ...

    Abstract Background: Brucellosis is the most common bacterial zoonosis, and serological tests are routinely used in brucellosis control and eradication programs. In order to improve the accuracy of serological diagnostic method used in bovine brucellosis detection, this study developed an improved competitive ELISA with higher specificity and good sensitivity.
    Results: This study prepared 12 monoclonal antibodies against smooth Brucella lipopolysaccharide. One monoclonal antibody 3 F9, presented C epitope specificity, was used to develop a competitive ELISA for the serological detection of bovine brucellosis. The competitive ELISA, a commercial competitive ELISA kit, the rose-bengal plate agglutination test, and a microplate agglutination test were all used in the detection of 6 hyperimmune antisera against other commonly cross-reacted bacterial pathogens and 110 clinical bovine serum samples. The results of the test comparisons indicated that the competitive ELISA had higher specificity than the commercial competitive ELISA kit and RBT, and comparable sensitivity with the commercial ELISA kit.
    Conclusions: This study provided a valuable detection tool with high specificity and good sensitivity, which prevent the wrong-culling of bovines in the eradication campaigns of bovine brucellosis.
    Mesh-Begriff(e) Animals ; Antibodies, Monoclonal/immunology ; Antibody Specificity ; Brucellosis, Bovine/diagnosis ; Cattle ; Enzyme-Linked Immunosorbent Assay/methods ; Enzyme-Linked Immunosorbent Assay/veterinary ; Epitopes ; Immunoglobulin G ; Immunoglobulin M ; Lipopolysaccharides/immunology ; Mice ; Sensitivity and Specificity
    Chemische Substanzen Antibodies, Monoclonal ; Epitopes ; Immunoglobulin G ; Immunoglobulin M ; Lipopolysaccharides
    Sprache Englisch
    Erscheinungsdatum 2015-05-21
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1746-6148
    ISSN (online) 1746-6148
    DOI 10.1186/s12917-015-0436-3
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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