LIVIVO - Search results -

Search results

Result 1 - 10 of total 176

Search options

Article: Gene Electrotransfer Efficiency in 2D and 3D Cancer Cell Models Using Different Electroporation Protocols: A Comparative Study.

de Caro, Alexia / Bellard, Elisabeth / Kolosnjaj-Tabi, Jelena / Golzio, Muriel / Rols, Marie-Pierre

2023 Volume 15, Issue 3

Abstract: Electroporation, a method relying on a pulsed electric field to induce transient cell membrane permeabilization, can be used as a non-viral method to transfer genes in vitro and in vivo. Such transfer holds great promise for cancer treatment, as it can ... ...

Abstract	Electroporation, a method relying on a pulsed electric field to induce transient cell membrane permeabilization, can be used as a non-viral method to transfer genes in vitro and in vivo. Such transfer holds great promise for cancer treatment, as it can induce or replace missing or non-functioning genes. Yet, while efficient in vitro, gene-electrotherapy remains challenging in tumors. To assess the differences of gene electrotransfer in respect to applied pulses in multi-dimensional (2D, 3D) cellular organizations, we herein compared pulsed electric field protocols applicable to electrochemotherapy and gene electrotherapy and different "High Voltage-Low Voltage" pulses. Our results show that all protocols can result in efficient permeabilization of 2D- and 3D-grown cells. However, their efficiency for gene delivery varies. The gene-electrotherapy protocol is the most efficient in cell suspensions, with a transfection rate of about 50%. Conversely, despite homogenous permeabilization of the entire 3D structure, none of the tested protocols allowed gene delivery beyond the rims of multicellular spheroids. Taken together, our findings highlight the importance of electric field intensity and the occurrence of cell permeabilization, and underline the significance of pulses' duration, impacting plasmids' electrophoretic drag. The latter is sterically hindered in 3D structures and prevents the delivery of genes into spheroids' core.
Language	English
Publishing date	2023-03-21
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2527217-2
ISSN	1999-4923
ISSN	1999-4923
DOI	10.3390/pharmaceutics15031004
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article ; Online: Therapeutic perspectives of high pulse repetition rate electroporation.

de Caro, Alexia / Talmont, Franck / Rols, Marie-Pierre / Golzio, Muriel / Kolosnjaj-Tabi, Jelena

Bioelectrochemistry (Amsterdam, Netherlands)

2023 Volume 156, Page(s) 108629

Abstract: Electroporation, a technique that uses electrical pulses to temporarily or permanently destabilize cell membranes, is increasingly used in cancer treatment, gene therapy, and cardiac tissue ablation. Although the technique is efficient, patients report ... ...

Abstract	Electroporation, a technique that uses electrical pulses to temporarily or permanently destabilize cell membranes, is increasingly used in cancer treatment, gene therapy, and cardiac tissue ablation. Although the technique is efficient, patients report discomfort and pain. Current strategies that aim to minimize pain and muscle contraction rely on the use of pharmacological agents. Nevertheless, technical improvements might be a valuable tool to minimize adverse events, which occur during the application of standard electroporation protocols. One recent technological strategy involves the use of high pulse repetition rate. The emerging technique, also referred as "high frequency" electroporation, employs short (micro to nanosecond) mono or bipolar pulses at repetition rate ranging from a few kHz to a few MHz. This review provides an overview of the historical background of electric field use and its development in therapies over time. With the aim to understand the rationale for novel electroporation protocols development, we briefly describe the physiological background of neuromuscular stimulation and pain caused by exposure to pulsed electric fields. Then, we summarize the current knowledge on electroporation protocols based on high pulse repetition rates. The advantages and limitations of these protocols are described from the perspective of their therapeutic application.
MeSH term(s)	Humans ; Electroporation/methods ; Cell Membrane/metabolism ; Cell Membrane Permeability ; Pain/metabolism ; Electricity
Language	English
Publishing date	2023-12-21
Publishing country	Netherlands
Document type	Review ; Journal Article
ZDB-ID	2010650-6
ISSN	1878-562X ; 0302-4598 ; 1567-5394
ISSN (online)	1878-562X
ISSN	0302-4598 ; 1567-5394
DOI	10.1016/j.bioelechem.2023.108629
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1178: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Micro-fragmented adipose tissue injection associated with arthroscopic procedures in patients with symptomatic knee osteoarthritis.

Cattaneo, G / De Caro, A / Napoli, F / Chiapale, D / Trada, P / Camera, A

BMC musculoskeletal disorders

2018 Volume 19, Issue 1, Page(s) 176

Abstract: Background: The social impact of degenerative diseases is steadily increasing, because of the continued rise in the mean age of the active population. Articular cartilage lesions are generally associated with disability and symptoms such as joint pain ... ...

Abstract	Background: The social impact of degenerative diseases is steadily increasing, because of the continued rise in the mean age of the active population. Articular cartilage lesions are generally associated with disability and symptoms such as joint pain and reduced function, and remain a challenge for the orthopaedic surgeon. Several non-invasive solution have been proposed, but the results achieved to date are far from being completely satisfactory. Recently, new therapeutic approaches, such as the use of mesenchymal stem cells, have been developed. Among the many sources, the adipose tissue is nowadays considered one of the smartest, due to its abundance and easy access. The aim of this retrospective study is to explore whether patients affected by symptomatic knee osteoarthritis treated with micro-fragmented adipose tissue associated with a chondral shaving procedure experience an improvement in symptoms and function. Methods: Thirty-eight patients affected by symptomatic knee osteoarthritis were treated in 2015 with an arthroscopic procedure associated with an injection of autologous and micro-fragmented adipose tissue. Micro-fragmented adipose tissue was obtained using a minimal manipulation technique in a closed system. Clinical outcomes were determined at 1, 3, 6, and 12 months follow-up using Knee Injury and Osteoarthritis Outcome Score questionnaire and direct physical examination. Safety of the procedure, recording type and incidence of any adverse event, was also assessed. Results: A steady and statistically significant improvement of all the clinical scores from pre-operative evaluation to 1, 3, 6, and 12 months follow-up was observed, with KOOS sport and quality of life being the most improved scores. On average, 92% of the patients clinically improved and 100% of them were satisfied with the treatment. No adverse events nor relevant complications were recorded. Conclusion: The result of the study pointed to micro-fragmented adipose tissue as a safe and beneficial adjuvant in the surgical treatment of degenerative knee chondropathy. The procedure is simple, sustainable, quick, minimally invasive, one-step, and safe. After one year, the results are very satisfactory and promising. A longer follow-up is needed to draw definitive conclusions and enlarge the indications. Trial registration: Registered at clinicaltrials.gov as NCT03527693 on 27 April 2018 (retrospectively registered).
MeSH term(s)	Adipose Tissue/transplantation ; Adult ; Arthroscopy/methods ; Female ; Humans ; Injections, Intra-Articular/methods ; Male ; Middle Aged ; Osteoarthritis, Knee/diagnosis ; Osteoarthritis, Knee/therapy ; Retrospective Studies ; Transplantation, Autologous/methods
Language	English
Publishing date	2018-05-30
Publishing country	England
Document type	Journal Article
ISSN	1471-2474
ISSN (online)	1471-2474
DOI	10.1186/s12891-018-2105-8
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Single-Molecule Sandwich Aptasensing on Nanoarrays by Tethered Particle Motion Analysis.

Soukarié, Diana / Rousseau, Philippe / Salhi, Maya / de Caro, Alexia / Escudier, Jean-Marc / Tardin, Catherine / Ecochard, Vincent / Salomé, Laurence

Analytical chemistry

2022 Volume 94, Issue 10, Page(s) 4319–4327

Abstract: High-throughput single-molecule techniques are expected to challenge the demand for rapid, simple, and sensitive detection methods in health and environmental fields. Based on a single-DNA-molecule biochip for the parallelization of tethered particle ... ...

Abstract	High-throughput single-molecule techniques are expected to challenge the demand for rapid, simple, and sensitive detection methods in health and environmental fields. Based on a single-DNA-molecule biochip for the parallelization of tethered particle motion analyses by videomicroscopy coupled to image analysis and its smart combination with aptamers, we successfully developed an aptasensor enabling the detection of single target molecules by a sandwich assay. One aptamer is grafted to the nanoparticles tethered to the surface by a long DNA molecule bearing the second aptamer in its middle. The detection and quantification of the target are direct. The recognition of the target by a pair of aptamers leads to a looped configuration of the DNA-particle complex associated with a restricted motion of the particles, which is monitored in real time. An analytical range extending over 3 orders of magnitude of target concentration with a limit of detection in the picomolar range was obtained for thrombin.
MeSH term(s)	Aptamers, Nucleotide ; Biosensing Techniques/methods ; DNA ; Limit of Detection ; Microarray Analysis ; Thrombin/analysis
Chemical Substances	Aptamers, Nucleotide ; DNA (9007-49-2) ; Thrombin (EC 3.4.21.5)
Language	English
Publishing date	2022-02-28
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.1c04995
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 4033: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Correlation between epicardial adipose tissue and atrial fibrillation burden in coronary artery bypass graft surgery.

Bolzan, Bruna / Morani, Giovanni / Rizzati, Vanni / Zamboni, Mauro / Mazzali, Gloria / Franzese, Ilaria / Tomasi, Luca / Mugnai, Giacomo / De Manna, Davide / Benfari, Giovanni / De Caro, Annamaria / Cortinovis, Matteo / Faggian, Giuseppe / Luciani, Giovanni Battista / Ribichini, Flavio Luciano

Journal of cardiovascular medicine (Hagerstown, Md.)

2023 Volume 24, Issue 4, Page(s) 253–260

Abstract: Aims: Recent studies suggest an association between epicardial adipose tissue (EAT) and atrial fibrillation. The aim of the study is to evaluate the quantitative and qualitative characteristics of EAT in relation to atrial fibrillation burden after ... ...

Abstract	Aims: Recent studies suggest an association between epicardial adipose tissue (EAT) and atrial fibrillation. The aim of the study is to evaluate the quantitative and qualitative characteristics of EAT in relation to atrial fibrillation burden after coronary artery bypass graft (CABG). Methods: This prospective single-centre study included patients undergoing CABG. The patients underwent transthoracic echocardiography and collection of a bioptic sample containing right appendage and EAT during CABG for histological characterization. After surgery, clinical and telemetry data were collected. Results: Fifty-six consecutive patients were enrolled. The mean postsurgical hospitalization was 7.9 ± 3.7 days. Twenty-two patients had at least one episode of atrial fibrillation. In the atrial fibrillation group, there was a bigger atrial volume, a higher degree of diastolic disfunction, a thicker layer of EAT and an older median age in comparison with the group that did not develop it. EAT with a cut-off of 4 mm was a predictor of atrial fibrillation with an odds ratio (OR) of 1.49 (confidence interval (CI) 1.09-2.04), 73% of sensibility and 89% of specificity. From the histological analyses, the patients with atrial fibrillation had a significantly higher percentage of fibrosis. At univariate analysis, atrial volume [OR 1.05, CI 1.01-1.09, P = 0.022], E/A rate (OR 0.04, CI 0.02-0.72 P = 0.29), the percentage of fibrosis (OR 1.12, CI 1.00-1.25, P = 0.045) and age (OR 1.17, CI 1.07-1.28, P = 0.001) were predictors of atrial fibrillation. At multivariate analysis, atrial volume (P = 0.027), fibrosis (P = 0.003) and age (P = 0.039) were independent predictors of atrial fibrillation. Conclusion: Postcardiac surgical atrial fibrillation is frequent. EAT thickness, atrial volume, fibrosis and age are predictors of postcardiac surgical atrial fibrillation.
MeSH term(s)	Humans ; Atrial Fibrillation/diagnosis ; Atrial Fibrillation/etiology ; Prospective Studies ; Coronary Artery Bypass/adverse effects ; Fibrosis ; Adipose Tissue/diagnostic imaging
Language	English
Publishing date	2023-03-23
Publishing country	United States
Document type	Journal Article
ZDB-ID	2223461-5
ISSN	1558-2035 ; 1558-2027
ISSN (online)	1558-2035
ISSN	1558-2027
DOI	10.2459/JCM.0000000000001455
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 5210: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 559: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: What nursing home environment can maximise eating independence among residents with cognitive impairment? Findings from a secondary analysis.

Palese, Alvisa / Gonella, Silvia / Grassetti, Luca / Longobardi, Melania / De Caro, Alessandro / Achil, Illarj / Hayter, Mark / Watson, Roger

Geriatric nursing (New York, N.Y.)

2020 Volume 41, Issue 6, Page(s) 709–716

Abstract: To explore the influence of the Nursing Home (NH) environment on eating independence while taking into account individual and nursing care factors, was the aim of the study. A secondary analysis was performed based on data collected in a multicentre ... ...

Abstract	To explore the influence of the Nursing Home (NH) environment on eating independence while taking into account individual and nursing care factors, was the aim of the study. A secondary analysis was performed based on data collected in a multicentre prospective observational study involving 13 NHs. Residents aged >65 were included (n = 1,027). Dependence in eating was measured using the Edinburgh Feeding Evaluation in Dementia scale (EdFED, range 0-20). In addition to individual and nursing care variables, the NHs environments were assessed with the Therapeutic Environment Screening Survey for Nursing Homes (TESS-NH, range 0-149). The mean EdFED score was 2.48 (95% Confidence Interval [CI]=2.22-2.73) and the TESS-NH score was 122.19 (95% CI=115.89-128.49). A linear regression analysis explained 30.8% of the total variance in eating dependence. Alongside individual and nursing care factors, in poor NH unit environments, residents with severe cognitive impairment showed increased eating dependence; in contrast, in better environments, similar residents showed maximal eating performance.
MeSH term(s)	Aged ; Cognitive Dysfunction ; Dementia ; Humans ; Nursing Homes ; Skilled Nursing Facilities ; Surveys and Questionnaires
Language	English
Publishing date	2020-05-13
Publishing country	United States
Document type	Journal Article ; Multicenter Study ; Observational Study
ZDB-ID	632559-2
ISSN	1528-3984 ; 0197-4572
ISSN (online)	1528-3984
ISSN	0197-4572
DOI	10.1016/j.gerinurse.2020.03.020
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 2789: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Pentoxifylline in prosthetic valve: a case report.

Disegna, E / De Caro, A / Nori, N / Taylor, M / Fantin, F / Mazzali, G / Zoico, E / Marchiotto, S / Zamboni, M / Gattazzo, S / Rossi, A P

Aging clinical and experimental research

2018 Volume 31, Issue 3, Page(s) 431–434

MeSH term(s)	Aged ; Female ; Heart Valve Prosthesis/adverse effects ; Hemolysis/drug effects ; Humans ; Pentoxifylline/administration & dosage
Chemical Substances	Pentoxifylline (SD6QCT3TSU)
Language	English
Publishing date	2018-06-11
Publishing country	Germany
Document type	Case Reports ; Journal Article
ZDB-ID	2104785-6
ISSN	1720-8319 ; 1594-0667
ISSN (online)	1720-8319
ISSN	1594-0667
DOI	10.1007/s40520-018-0983-0
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 3343: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article: Brown and Beige Adipose Tissue and Aging.

Zoico, Elena / Rubele, Sofia / De Caro, Annamaria / Nori, Nicole / Mazzali, Gloria / Fantin, Francesco / Rossi, Andrea / Zamboni, Mauro

Frontiers in endocrinology

2019 Volume 10, Page(s) 368

Abstract: Across aging, adipose tissue (AT) changes its quantity and distribution: AT becomes dysfunctional with an increase in production of inflammatory peptides, a decline of those with anti-inflammatory activity and infiltration of macrophages. Adipose organ ... ...

Abstract	Across aging, adipose tissue (AT) changes its quantity and distribution: AT becomes dysfunctional with an increase in production of inflammatory peptides, a decline of those with anti-inflammatory activity and infiltration of macrophages. Adipose organ dysfunction may lead to age-related metabolic alterations. Aging is characterized by an increase in adiposity and a decline in brown adipose tissue (BAT) depots and activity, and UCP1 expression. There are many possible links to age-associated involution of BAT, including the loss of mitochondrial function, impairment of the sympathetic nervous system, age-induced alteration of brown adipogenic stem/progenitor cell function and changes in endocrine signals. Aging is also associated with a reduction in beige adipocyte formation. Beige adipocytes are known to differentiate from a sub-population of progenitors resident in white adipose tissue (WAT); a defective ability of progenitor cells to proliferate and differentiate has been hypothesized with aging. The loss of beige adipocytes with age may be caused by changes in trophic factors in the adipose tissue microenvironment, which regulate progenitor cell proliferation and differentiation. This review focuses on possible mechanisms involved in the reduction of BAT and beige activity with aging, along with possible targets for age-related metabolic disease therapy.
Language	English
Publishing date	2019-06-20
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2592084-4
ISSN	1664-2392
ISSN	1664-2392
DOI	10.3389/fendo.2019.00368
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Diode Laser Photocoagulation of Intraoral and Perioral Venous Malformations After Tridimensional Staging by High Definition Ultrasonography.

Limongelli, Luisa / Tempesta, Angela / De Caro, Alessio / Maiorano, Eugenio / Angelelli, Giuseppe / Capodiferro, Saverio / Favia, Gianfranco

Photobiomodulation, photomedicine, and laser surgery

2019 Volume 37, Issue 11, Page(s) 722–728

Abstract: Objective: ...

Abstract	Objective:
MeSH term(s)	Adult ; Anesthesia, Local ; Female ; Humans ; Laser Coagulation/methods ; Lasers, Semiconductor ; Male ; Mouth/surgery ; Ultrasonography ; Vascular Malformations/diagnostic imaging ; Vascular Malformations/surgery
Language	English
Publishing date	2019-09-27
Publishing country	United States
Document type	Journal Article
ISSN	2578-5478
ISSN (online)	2578-5478
DOI	10.1089/photob.2019.4635
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Senolytic effects of quercetin in an in vitro model of pre-adipocytes and adipocytes induced senescence.

Zoico, Elena / Nori, Nicole / Darra, Elena / Tebon, Maela / Rizzatti, Vanni / Policastro, Gabriella / De Caro, Annamaria / Rossi, Andrea Petronio / Fantin, Francesco / Zamboni, Mauro

Scientific reports

2021 Volume 11, Issue 1, Page(s) 23237

Abstract: The dysfunction of adipose tissue with aging and the accumulation of senescent cells has been implicated in the pathophysiology of chronic diseases. Recently interventions capable of reducing the burden of senescent cells and in particular the ... ...

Abstract	The dysfunction of adipose tissue with aging and the accumulation of senescent cells has been implicated in the pathophysiology of chronic diseases. Recently interventions capable of reducing the burden of senescent cells and in particular the identification of a new class of drugs termed senolytics have been object of extensive investigation. We used an in vitro model of induced senescence by treating both pre-adipocytes as well as mature adipocytes with hydrogen peroxide (H
MeSH term(s)	3T3-L1 Cells ; Adipocytes/drug effects ; Aging/drug effects ; Animals ; Cellular Senescence/drug effects ; Hydrogen Peroxide ; Mice ; MicroRNAs/metabolism ; Oxidative Stress/drug effects ; Quercetin/pharmacology ; Senotherapeutics/pharmacology
Chemical Substances	MicroRNAs ; Senotherapeutics ; Quercetin (9IKM0I5T1E) ; Hydrogen Peroxide (BBX060AN9V)
Language	English
Publishing date	2021-12-01
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-021-02544-0
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

To top

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito