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Article ; Online: Effects of coenzyme Q10 and N-acetylcysteine on experimental poisoning by paracetamol in Wistar rats.

da Silva, Rayanne Henrique Santana / de Moura, Mariana / de Paula, Larissa / Arantes, Kelly Carolina / da Silva, Marina / de Amorim, Jaqueline / Miguel, Marina Pacheco / Martins, Danieli Brolo / de Melo E Silva, Daniela / Melo, Marília Martins / Botelho, Ana Flávia Machado

PloS one

2023 Volume 18, Issue 8, Page(s) e0290268

Abstract: Paracetamol (PAR) is a drug widely used in human and veterinary medicine as an analgesic and antipyretic, often involved in cases of intoxication. The most common clinical signs result from damage to red blood cells and hepatocytes, and this intoxication ...

Abstract	Paracetamol (PAR) is a drug widely used in human and veterinary medicine as an analgesic and antipyretic, often involved in cases of intoxication. The most common clinical signs result from damage to red blood cells and hepatocytes, and this intoxication is considered a model for the induction of acute liver failure. In the present study, the hepatoprotective effects of coenzyme Q10 (CoQ10) and N-acetylcysteine (NAC) against experimental paracetamol (PAR) poisoning were analysed. Thirty-five adult Wistar rats (Rattus novergicus albinus) were randomly assigned to five groups, and thirty-one of these survived the treatments. Negative control group (CON-) received 1mL of 0.9% NaCl orally (PO). Other groups received 1.2g/kg of PAR (PO). Positive control group (CON+) received only PAR. NAC group received 800 mg/kg intraperitoneally (IP) of NAC 1h after the administration of PAR and at 12 h received 1mL of 0.9% NaCl, IP. The fourth group (CoQ10) received 1h and 12 h after intoxication, CoQ10 (10mg/kg IP). And the fifth group (NAC+CoQ10) received NAC (800mg/kg, IP) and CoQ10 (10mg/kg, IP). After 12 hours, the rats were euthanized and necropsied to collect liver and kidney tissues for histopathological evaluation and electronic microscopy. A single dose of PAR caused severe acute hepatitis. NAC couldn't reverse the liver and kidney damages. The group that received CoQ10 and NAC had moderate liver damage, while the group that received only CoQ10 had lower values of liver enzymes and mild liver and kidney damage. Animals that received treatment with CoQ10 or NAC+CoQ10 presented normal hepatocyte mitochondria and nuclei. Although CoQ10 couldn't reverse PAR organ damage, results indicate promising hepatoprotection in Wistar rats.
MeSH term(s)	Adult ; Humans ; Rats ; Animals ; Acetylcysteine/pharmacology ; Acetylcysteine/therapeutic use ; Acetaminophen ; Rats, Wistar ; Saline Solution
Chemical Substances	Acetylcysteine (WYQ7N0BPYC) ; Acetaminophen (362O9ITL9D) ; coenzyme Q10 (EJ27X76M46) ; Saline Solution
Language	English
Publishing date	2023-08-22
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0290268
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Experiments match simulations in a multiple post reentrant cavity.

de Paula, L A N / Goryachev, M / Tobar, M E

The Review of scientific instruments

2017 Volume 88, Issue 12, Page(s) 125104

Abstract: Microwave reentrant cavities are used for many applications in science and engineering. The potential for both high mechanical tunability and high electric quality factors make them important tools in many areas. They are usually resonant cylindrical ... ...

Abstract	Microwave reentrant cavities are used for many applications in science and engineering. The potential for both high mechanical tunability and high electric quality factors make them important tools in many areas. They are usually resonant cylindrical cavities with a central post, which makes a small gap spacing with the cavity wall. By adding an arbitrary number of extra posts, they are generalized to a type of multiple post reentrant cavity. This new approach has been theoretically studied but no experimental results have been presented. The main purpose of this work was to compare experimental modes with simulated ones from a reentrant cavity made of forty nine cylindrical posts. Each post could be moved using a screw in order to make tunable gap spacing between the post top and the cavity cover. Eight different gap setups were made making it possible to investigate thirty six different reentrant modes at room temperature. The lowest frequency percentage agreement between experiment and simulation was 91.31%, and the best one was 99.92%. Taking into account all the modes, 94.44% of them agreed above 96%. Thus, we have determined an experimental procedure suitable to investigate the reentrant modes from multiple post cavities. There is a wide range of potential applications for such cavities due to their unique features compared to conventional ones.
Language	English
Publishing date	2017-12
Publishing country	United States
Document type	Journal Article
ZDB-ID	209865-9
ISSN	1089-7623 ; 0034-6748
ISSN (online)	1089-7623
ISSN	0034-6748
DOI	10.1063/1.4997626
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Article: Algorithms applied in decision-making for sustainable transport

Barcellos de Paula, Luciano / Fernando Augusto Silva Marins

Journal of cleaner production. 2018 Mar. 01, v. 176

2018

Abstract: Global warming, as many other threats, requires more activity from the government, and people inquire about this, rightfully so. However, the public and private sectors nowadays have a joint responsibility with society. Global warming has come out of the ...

Abstract	Global warming, as many other threats, requires more activity from the government, and people inquire about this, rightfully so. However, the public and private sectors nowadays have a joint responsibility with society. Global warming has come out of the scientific community and shows that climate change is an important social, economic, and political issue. Currently the carbon dioxide (CO2) emissions by transportation sector are the main polluter of the planet. For this reason, in this paper we propose a reflection on transport, which is an important sector of the economy, responsible for the generation of many jobs and wealth but, at the same time, it has negative impacts on people, companies, and the environment. Recent investigations demonstrate that mobility problems affects the life quality of the people, business productivity and gross domestic product (GDP) performance. In the face of the changes that we are experiencing, it is important to find alternatives that assistance governments in the decision-making to solve the problem of traffic in large and medium-size cities and bring well-being to the population. The goal is to provide accurate tool to help take the best decisions in situations that most of the time has a lack of economic resources and transparency. Another motivation is to increase the productivity of companies and achieve sustainable development by reducing mobility problems and CO2 emissions. As outcomes of the paper, an in-depth study is presented on the subject that indicates a gap in the literature with fuzzy logic applied to sustainability. We indicate a sustainable option for the transport sector using algorithms for decision-making, and broaden the discussion on climate change and solutions to reduce the impacts caused by humanity. When the models were applied through a conceptual study, it was possible to provide an instrument generated on the use of algorithms that can aid decision-making by getting qualitative data from a discussion with stakeholders on a topic. Finally, the document identifies the importance of the participation of stakeholder, reinforces legitimacy and transparency in decision-making processes and above all present a tool that can help governments to solve this problem. In short, this work proposes an original and valuable tool to be applied in the decision-making process for the sustainable transport sector based on the stakeholder engagement.
Keywords	algorithms ; business enterprises ; carbon dioxide ; cities ; decision making ; economic resources ; employment ; fuzzy logic ; global warming ; greenhouse gas emissions ; gross domestic product ; motivation ; politics ; society ; stakeholders ; sustainable development ; traffic
Language	English
Dates of publication	2018-0301
Size	p. 1133-1143.
Publishing place	Elsevier Ltd
Document type	Article
ISSN	0959-6526
DOI	10.1016/j.jclepro.2017.11.216
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Target selectivity of cholesterol-phosphatidylcholine liposome loaded with phthalocyanine for breast cancer diagnosis and treatment by photodynamic therapy.

Ceron Jayme, Cristiano / Rezende, Nayara / S Fernandes, Daniela / B de Paula, Leonardo / Gimenes de Castro, Bárbara / U Takahashi, Luandra Aparecida / Tedesco, Antonio Claudio

Photodiagnosis and photodynamic therapy

2022 Volume 39, Page(s) 102992

Abstract: This study investigated the ability of cholesterol-phosphatidylcholine liposomes loaded with chloride aluminum phthalocyanine (CL-AlClPc) to discriminate between healthy (MCF-10A) and neoplastic (MCF-7 and MDA-MB-231) breast cells for breast cancer ... ...

Abstract	This study investigated the ability of cholesterol-phosphatidylcholine liposomes loaded with chloride aluminum phthalocyanine (CL-AlClPc) to discriminate between healthy (MCF-10A) and neoplastic (MCF-7 and MDA-MB-231) breast cells for breast cancer diagnosis and treatment by photodynamic therapy (PDT) using a new drug delivery system consisting of CL-AlClPc. When PDT treatment was applied at an energy fluence of 700 mJ/cm², CL-AlClPc was more cytotoxic to neoplastic cells than to healthy breast cells because CL-AlClPc was better internalized by the tumor cells. An even higher fluorescence signal is expected for neoplastic cells during clinical treatment than for healthy cells, which will be useful for precise and targeted tumor cell detection. CL-AlClPc also facilitated better drug distribution and targeting of essential organelles inside the cells. This selectivity is critical for future in vivo diagnosis and treatment; it prevents side effects because it prioritizes tumor cells and tissues instead of healthy ones. The CL-AlClPc system designed herein had a small size (150 nm), low zeta potential (-6 mV), low polydispersity (0.16), high encapsulation rate efficiency (82.83%), and high shelf stability (12 months).
MeSH term(s)	Breast Neoplasms/diagnosis ; Breast Neoplasms/drug therapy ; Cell Line, Tumor ; Cholesterol ; Female ; Humans ; Isoindoles ; Liposomes ; Phosphatidylcholines ; Photochemotherapy/methods ; Photosensitizing Agents/pharmacology
Chemical Substances	Isoindoles ; Liposomes ; Phosphatidylcholines ; Photosensitizing Agents ; Cholesterol (97C5T2UQ7J) ; phthalocyanine (V5PUF4VLGY)
Language	English
Publishing date	2022-07-06
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2149918-4
ISSN	1873-1597 ; 1572-1000
ISSN (online)	1873-1597
ISSN	1572-1000
DOI	10.1016/j.pdpdt.2022.102992
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: High levels of anti-Leishmania IgG3 and low CD4

Kuschnir, Renata Caetano / Pereira, Leonardo Soares / Dutra, Maria Rita Teixeira / de Paula, Ludmila / Silva-Freitas, Maria Luciana / Corrêa-Castro, Gabriela / da Costa Cruz Silva, Simone / Cota, Glaucia / Santos-Oliveira, Joanna Reis / Da-Cruz, Alda Maria

BMC infectious diseases

2021 Volume 21, Issue 1, Page(s) 369

Abstract: Background: Visceral leishmaniasis (VL) is severe and potentially fatal. Brazil is one of the countries with the greatest endemicity for the disease in the world. The reduction of CD4: Methods: Fifteen VL patients recruited from Hospital Eduardo de ... ...

Abstract	Background: Visceral leishmaniasis (VL) is severe and potentially fatal. Brazil is one of the countries with the greatest endemicity for the disease in the world. The reduction of CD4 Methods: Fifteen VL patients recruited from Hospital Eduardo de Menezes (BH-MG) were grouped into relapsing (R-VL, n = 5) and non-relapsing (NR-VL, n = 10) and evaluated during active disease, immediately after treatment (post-treatment) and 6 months post-treatment (6mpt). Clinical and laboratory data obtained from medical records were correlated with CD4 Results: During the active phase of VL, despite similarity in the clinical symptoms, the rates of thrombocytopenia, elevated transaminases (AST and ALT) and hyperbilirubinemia were higher in the NR-VL group compared to R-VL (p < 0.05), a profile reversed during the post-treatment phase. All patients had low CD4 Conclusions: During active phase of VL, the NR-VL patients presented more severe laboratorial abnormalities compared to R-VL, probably because the latter had already received previous treatment. On the other hand, R-VL exhibited greater impairment of immune reconstitution and a high degree of B lymphocyte activation, which must be a factor that favored relapses.
MeSH term(s)	Adult ; Amphotericin B/therapeutic use ; Antibodies, Protozoan/blood ; Brazil ; CD4-Positive T-Lymphocytes/cytology ; CD4-Positive T-Lymphocytes/immunology ; CD4-Positive T-Lymphocytes/metabolism ; Deoxycholic Acid/therapeutic use ; Drug Combinations ; Female ; HIV Infections/complications ; Humans ; Immunoglobulin G/blood ; Interleukin-6/blood ; Leishmania/immunology ; Leishmaniasis, Visceral/drug therapy ; Leishmaniasis, Visceral/immunology ; Leishmaniasis, Visceral/pathology ; Male ; Middle Aged ; Recurrence
Chemical Substances	Antibodies, Protozoan ; Drug Combinations ; Immunoglobulin G ; Interleukin-6 ; Deoxycholic Acid (005990WHZZ) ; Amphotericin B (7XU7A7DROE) ; amphotericin B, deoxycholate drug combination (87687-70-5)
Language	English
Publishing date	2021-04-20
Publishing country	England
Document type	Journal Article
ISSN	1471-2334
ISSN (online)	1471-2334
DOI	10.1186/s12879-021-06051-5
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Article ; Online: Electrostatic frequency reduction: A negative stiffness mechanism for measuring dissipation in a mechanical oscillator at low frequency.

Erwin, A / Stone, K J / Shelton, D / Hahn, I / Huie, W / de Paula, L A N / Schmerr, N C / Paik, H J / Chui, T C P

The Review of scientific instruments

2021 Volume 92, Issue 1, Page(s) 15101

Abstract: Broadband seismometers and gravitational wave detectors make use of mechanical resonators with a high quality factor to reduce Brownian noise. At low frequency, Brownian noise is ultimately dominated by internal friction in the suspension, which has a 1/ ... ...

Abstract	Broadband seismometers and gravitational wave detectors make use of mechanical resonators with a high quality factor to reduce Brownian noise. At low frequency, Brownian noise is ultimately dominated by internal friction in the suspension, which has a 1/f noise compared with the white noise arising from viscous dissipation. Internal friction is typically modeled as a frequency-dependent loss and can be challenging to measure reliably through experiment. In this work, we present the physics and experimental implementation of electrostatic frequency reduction (EFR) in a mechanical oscillator-a method to measure dissipation as a function of frequency. By applying a high voltage to two parallel capacitor plates, with the center plate being a suspended mass, an electrostatic force is created that acts as a negative stiffness mechanism to reduce the system's resonance frequency. Through EFR, the loss angle can be measured as a function of frequency by measuring amplitude decay response curves for a range of applied voltages. We present experimental measurements of the loss angle for three metal helical extension springs in the nominal frequency range 0.7-2.9 Hz at 0.2 Hz intervals, demonstrating the possibility for fine adjustment of the resonance frequency for loss angle measurements. A quality factor proportional to the resonance frequency squared was measured, an indication that internal friction and other non-viscous dissipation elements, such as electrostatic damping, were the prominent loss mechanisms in our experiments. Finally, we consider the implications of Brownian noise arising from internal friction on a low 1/f noise seismometer.
Language	English
Publishing date	2021-01-27
Publishing country	United States
Document type	Journal Article
ZDB-ID	209865-9
ISSN	1089-7623 ; 0034-6748
ISSN (online)	1089-7623
ISSN	0034-6748
DOI	10.1063/5.0019351
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Article: Evaluation of a chloroaluminium phthalocyanine-loaded magnetic nanoemulsion as a drug delivery device to treat glioblastoma using hyperthermia and photodynamic therapy

de Paula, L. B / F. L. Primo / M. R. Pinto / P. C. Morais / A. C. Tedesco

RSC advances. 2017 Jan. 30, v. 7, no. 15

2017

Abstract: This study reports the production of magnetic nanoemulsions (MNEs) loaded with citrate-coated maghemite nanoparticles (0.15 × 10¹⁶ or 1.50 × 10¹⁶ particle per mL) and chloroaluminum-phthalocyanine (0.05 mg mL⁻¹). Using different cell line models (BM-MSC, ...

Abstract	This study reports the production of magnetic nanoemulsions (MNEs) loaded with citrate-coated maghemite nanoparticles (0.15 × 10¹⁶ or 1.50 × 10¹⁶ particle per mL) and chloroaluminum-phthalocyanine (0.05 mg mL⁻¹). Using different cell line models (BM-MSC, U87MG, and T98G) an in vitro test was performed to assess the cell viability while incubating the cells with the two prepared formulations, before and after performing hyperthermia (HPT: 1 MHz frequency, 40 Oe magnetic field amplitude) and photodynamic therapy (PDT at 670 nm wavelength, 700 mJ cm⁻² energy density). We found from all cell lines that under the HPT treatment the cell viability reduction has averaged 15%, regardless the magnetic nanoparticle content within the MNE. Using both MNE formulation (0.15 × 10¹⁶ or 1.54 × 10¹⁶ magnetic particle per mL) and applying only PDT light treatment we reach an average decrease of 52%. However, a total reduction of about 70% was found while combining the HPT and PDT treatments. Confocal studies clearly indicated the cytoplasm localization and active site of the drug delivery device. Therefore, the combined treatment of HPT and PDT represents a promising paradigm for brain cancer intervention, such as glioblastoma.
Keywords	active sites ; brain neoplasms ; cell lines ; cell viability ; cytoplasm ; drugs ; energy density ; fever ; glioblastoma ; in vitro studies ; maghemite ; magnetic fields ; models ; nanoemulsions ; nanoparticles ; photochemotherapy ; wavelengths
Language	English
Dates of publication	2017-0130
Size	p. 9115-9122.
Publishing place	The Royal Society of Chemistry
Document type	Article
ISSN	2046-2069
DOI	10.1039/c6ra26105a
Database	NAL-Catalogue (AGRICOLA)

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Article: Synergism of citral from Cymbopogon citratus essential oil and undecan-2-one against Meloidogyne incognita

Barros, A. F. / Campos, V. P. / de Paula, L. L. / de Oliveira, D. F. / Silva, G. H.

Nematology

2020 Volume 22, Issue 10, Page(s) 1101

Language	English
Document type	Article
ZDB-ID	1450013-9
ISSN	1388-5545
Database	Current Contents Nutrition, Environment, Agriculture

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Article ; Online: Unequal Impact of

Journal of dental research

2023 Volume 102, Issue 6, Page(s) 616–625

Abstract: Dentinogenesis imperfecta (DI) is the main orodental manifestation of osteogenesis imperfecta (OI) caused ... ...

Abstract	Dentinogenesis imperfecta (DI) is the main orodental manifestation of osteogenesis imperfecta (OI) caused by
MeSH term(s)	Humans ; Collagen Type I/genetics ; Collagen Type I, alpha 1 Chain ; Dentinogenesis Imperfecta/genetics ; Genetic Association Studies ; Mutation ; Osteogenesis Imperfecta/diagnostic imaging ; Osteogenesis Imperfecta/genetics
Chemical Substances	COL1A2 protein, human ; Collagen Type I ; Collagen Type I, alpha 1 Chain ; Collagen Type I, alpha2 Subunit ; COL1A1 protein, human
Language	English
Publishing date	2023-03-23
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80207-4
ISSN	1544-0591 ; 0022-0345
ISSN (online)	1544-0591
ISSN	0022-0345
DOI	10.1177/00220345231154569
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Ul III Zs.75: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
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Article ; Online: IFN-γ +875 microsatellite polymorphism as a potential protection marker for leprosy patients from Amazonas state, Brazil.

Silva, G A V / Santos, M P / Mota-Passos, I / Boechat, A L / Malheiro, A / Naveca, F G / de Paula, L

Cytokine

2012 Volume 60, Issue 2, Page(s) 493–497

Abstract: Polymorphisms present in the first intron of IFN-γ may have an important role in the regulation of the immune response, which could have functional consequences for gene transcription. Leprosy patients are characterized by different immune responses in ... ...

Abstract	Polymorphisms present in the first intron of IFN-γ may have an important role in the regulation of the immune response, which could have functional consequences for gene transcription. Leprosy patients are characterized by different immune responses in different clinical forms. We investigated a possible association of the +874 polymorphism and CA repeats present in the first intron of IFN-γ with susceptibility to leprosy and with the manifestation of the different clinical forms. Nucleotide sequencing was performed with samples from 108 leprosy patients and 113 controls subjects, as well as immunophenotyping of CD(4)(+), CD(8)(+) and CD(69)(+) T cells by flow cytometry. The data showed that there were no significant differences between patients and control subjects, as well as according classification of Ridley-Jopling. However, the A/A genotype was significantly increased in paucibacillary patients (p=0.028) and the microsatellite encoding 16 CA repeats were significantly associated with paucibacillary compared to multibacillary patients (p=0.019). Individuals homozygous for the +874 A allele, the mean level of CD(4)(+) and CD(69)(+) T cells was higher. Our data suggest that polymorphisms present in the first intron of IFN-γ are not associated with susceptibility to leprosy, nevertheless, the +874 polymorphism and the CA repeats number encoded in IFN-γ gene may be related to a higher cellular immune response in patients and are consistently more frequently detected in PB patients.
MeSH term(s)	Adolescent ; Adult ; Aged ; Alleles ; Biomarkers/metabolism ; Brazil ; Case-Control Studies ; Female ; Gene Frequency/genetics ; Genetic Predisposition to Disease ; Humans ; Interferon-gamma/genetics ; Leprosy/genetics ; Leprosy/prevention & control ; Lymphocyte Subsets/immunology ; Male ; Microsatellite Repeats/genetics ; Middle Aged ; Polymorphism, Single Nucleotide/genetics ; Young Adult
Chemical Substances	Biomarkers ; IFNG protein, human ; Interferon-gamma (82115-62-6)
Language	English
Publishing date	2012-11
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1018055-2
ISSN	1096-0023 ; 1043-4666
ISSN (online)	1096-0023
ISSN	1043-4666
DOI	10.1016/j.cyto.2012.04.043
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