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Article ; Online: Characterization of an antimicrobial pentapeptide produced by a drug-sensitive Pseudomonas aeruginosa strain PAST18.

Deb, Saikat / Dutta, Tapan K

Biochemical and biophysical research communications

2023 Volume 663, Page(s) 78–86

Abstract: The members of the genus Pseudomonas can secrete a wide range of ribosomally encoded antagonistic peptides and proteins, ranging from small microcins to large tailocins. In this study, a drug-sensitive Pseudomonas aeruginosa strain isolated from a high- ... ...

Abstract	The members of the genus Pseudomonas can secrete a wide range of ribosomally encoded antagonistic peptides and proteins, ranging from small microcins to large tailocins. In this study, a drug-sensitive Pseudomonas aeruginosa strain isolated from a high-altitude virgin soil sample showed a broad range of antibacterial activity against Gram-positive and Gram-negative bacteria. The antimicrobial compound, purified by affinity chromatography, ultrafiltration, and high-performance liquid chromatography, showed a molecular weight (M + H)
MeSH term(s)	Anti-Bacterial Agents/chemistry ; Pseudomonas aeruginosa/metabolism ; Gram-Negative Bacteria ; Gram-Positive Bacteria ; Anti-Infective Agents ; Microbial Sensitivity Tests
Chemical Substances	Anti-Bacterial Agents ; Anti-Infective Agents
Language	English
Publishing date	2023-04-21
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	205723-2
ISSN	1090-2104 ; 0006-291X ; 0006-291X
ISSN (online)	1090-2104 ; 0006-291X
ISSN	0006-291X
DOI	10.1016/j.bbrc.2023.04.069
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Article ; Online: Characterization of an antimicrobial pentapeptide produced by a drug-sensitive Pseudomonas aeruginosa strain PAST18

Deb, Saikat / Dutta, Tapan K.

Biochemical and Biophysical Research Communications.

2023

Abstract	The members of the genus Pseudomonas can secrete a wide range of ribosomally encoded antagonistic peptides and proteins, ranging from small microcins to large tailocins. In this study, a drug-sensitive Pseudomonas aeruginosa strain isolated from a high-altitude virgin soil sample showed a broad range of antibacterial activity against Gram-positive and Gram-negative bacteria. The antimicrobial compound, purified by affinity chromatography, ultrafiltration, and high-performance liquid chromatography, showed a molecular weight (M + H)⁺ of 494.7667 daltons, as revealed by ESI-MS analysis. The MS-MS analysis divulged the compound as an antimicrobial pentapeptide with the sequence NH₂-Thr-Leu-Ser-Ala-Cys-COOH (TLSAC) and was further verified by evaluating the antimicrobial activity of the chemically synthesized pentapeptide. The extracellularly released pentapeptide, which is relatively hydrophobic in nature, is encoded in a symporter protein, as appraised from the whole genome sequence analysis of strain PAST18. The influence of different environmental factors was examined to determine the stability of the antimicrobial peptide (AMP), which was also assessed for several other biological functions, including antibiofilm activity. Further, the antibacterial mechanism of the AMP was evaluated by a permeability assay. Overall, the characterised pentapeptide, as revealed in this study, may find use as a potential biocontrol agent in various commercial applications.
Keywords	Pseudomonas aeruginosa ; affinity chromatography ; altitude ; anti-infective agents ; antibacterial properties ; antimicrobial peptides ; biological control agents ; high performance liquid chromatography ; hydrophobicity ; molecular weight ; nucleotide sequences ; permeability ; research ; sequence analysis ; symporters ; ultrafiltration ; virgin soils ; Antimicrobial peptide ; Pentapeptide ; Antibiofilm activity ; ESI-MS ; Biocontrol agent
Language	English
Publishing place	Elsevier Inc.
Document type	Article ; Online
Note	Pre-press version
ZDB-ID	205723-2
ISSN	0006-291X ; 0006-291X
ISSN (online)	0006-291X
ISSN	0006-291X
DOI	10.1016/j.bbrc.2023.04.069
Database	NAL-Catalogue (AGRICOLA)

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Article: Passenger's perception about city buses in the aftermath of COVID 19: Experience from Guwahati city, India.

Deb, Saikat / Hinge, Gilbert

International journal of disaster risk reduction : IJDRR

2022 Volume 85, Page(s) 103489

Abstract: The COVID-19 epidemic has affected public transport in various cities worldwide, including Guwahati city in India, where a 59.09% decline in the ridership of city bus services occurred in autumn 2021. The present work aims to investigate the impact of ... ...

Abstract	The COVID-19 epidemic has affected public transport in various cities worldwide, including Guwahati city in India, where a 59.09% decline in the ridership of city bus services occurred in autumn 2021. The present work aims to investigate the impact of COVID-19 on travelers' perception and identify the parameters associated with the user's dissatisfaction for improving bus service in Guwahati city. In addition, the study also estimated the revenue loss of bus services due to this pandemic. This was carried out based on survey data from travelers in pre-covid (January 2019) situation, unlocked with 50% seating capacity (November 2020), and unlocked with 100% seat capacity plus some limited amount of standee (January 2021) situation. Results showed an increase in the number of users' priorities in post covid compared to pre covid conditions. Factors such as comfort, fairness, reliability & convenience, and safety are the priority areas of users' pre covid. Whereas bus service attribute such as vehicle condition & hygiene emerged as an additional priority of users post covid. Unfortunately, the bus service in the city performed poorly in terms of hygiene, leading to the shifting of the user to other modes of transport. The poor condition of the vehicles is another major factor that made the users to discard the bus service. This led to huge financial losses of the public transit authorities. The cost analysis result showed that the city bus operators faced a financial loss due to the COVID19 outbreak. The average wage of the drivers reduced from 780 Rs./day to 339 Rs./day in the first unlock phase and 476 Rs./day in the second unlock phase. The present study discusses the introduction of the new route in the city, regular cleaning, regular servicing of the buses, and other recommendations to improve the bus service in Guwahati city.
Language	English
Publishing date	2022-12-14
Publishing country	England
Document type	Journal Article
ZDB-ID	2695877-6
ISSN	2212-4209
ISSN	2212-4209
DOI	10.1016/j.ijdrr.2022.103489
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Article ; Online: A Scanning Electron Microscope Evaluation of the Efficacy of Different Fluoride-releasing Dental Restorative Materials to Prevent Enamel Demineralization: An

Dhananjaya, Kiran M / Chakraborty, Mrinmoy / Vadavadagi, Suneel V / Sinha, Garima / Verma, Tanya / Deb, Saikat

The journal of contemporary dental practice

2021 Volume 22, Issue 11, Page(s) 1292–1296

Abstract: Aim: Aim of the present research was to investigate the effectiveness of various fluoride-releasing dental restorative agents in preventing demineralization of enamel.: Materials and methods: Eighty human mandibular permanent molar teeth constituted ... ...

Abstract	Aim: Aim of the present research was to investigate the effectiveness of various fluoride-releasing dental restorative agents in preventing demineralization of enamel. Materials and methods: Eighty human mandibular permanent molar teeth constituted the study group. All samples were subjected to storage in thymol, after which they were taken out to prepare alike proximal box in each. Inductions of artificial enamel surface lesions were done by placing the teeth in demineralizing solution for 96 hours. Subsequently, all 80 molars were randomly assigned to any of the four groups (i.e., 20 in every individual group) according to the restoration as group A: giomer (composite resin containing surface pre-reacted glass-ionomer fillers), group B: compomer (polyacid-modified composite resin), group C: resin-modified glass-ionomer cement (RMGIC), group D: fluoride-releasing composite. After this, the pH cycling was performed, and the samples were subjected to examination beneath scanning electron microscope (SEM). Results: Higher mean areas of remineralization were noted when RMGIC (96.34 ± 0.06) was used followed by the compomer (109.52 ± 0.17), giomer (118.39 ± 0.82), and the fluoride-releasing composite group (129.27 ± 0.31) in that order. A statistically significant difference was seen amid the investigational groups that utilized different restorative agents ( Conclusion: This research infers that the RMGIC-treated samples exhibited significantly superior performance in preventing enamel demineralization in comparison to compomer, giomer as well as fluoride-releasing composites. Clinical significance: One among the highly frequently employed anticariogenic materials is fluorides. Owing to this characteristic, they are integrated into numerous restorative substances. Nevertheless, the quantity and speed of fluoride release differ in different agents, which translates to the efficacy of the restorative agent in avoiding demineralization about the restoration.
MeSH term(s)	Dental Enamel/pathology ; Fluorides/therapeutic use ; Glass Ionomer Cements/chemistry ; Glass Ionomer Cements/therapeutic use ; Humans ; Microscopy, Electron, Scanning ; Tooth Demineralization/prevention & control
Chemical Substances	Glass Ionomer Cements ; Fluorides (Q80VPU408O)
Language	English
Publishing date	2021-11-01
Publishing country	India
Document type	Journal Article
ISSN	1526-3711
ISSN (online)	1526-3711
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Investor attention, analyst recommendation revisions, and stock prices

Welagedara, Venura / Harminder Singh / Deb, Saikat Sovan

Pacific-Basin finance journal Vol. 45 , p. 211-223

2017 Volume 45, Page(s) 211–223

Author's details	Venura Welagedara, Saikat Sovan Deb, Harminder Singh
Keywords	Analyst recommendation ; Price reaction ; Investor attention
Language	English
Publisher	Elsevier
Publishing place	Amsterdam [u.a.]
Document type	Article
ZDB-ID	1343420-2
ISSN	0927-538X
Database	ECONomics Information System

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Article: Effect of Acidic Beverages on Color Stability and Microhardness of Various Esthetic Restorative Materials: A Comparative Study.

Rathod, Aradhana / Vadavadagi, Suneel V / Verma, Tanya / Kumar, Pawan / Deepak, Potluri Venkataratna / Deb, Saikat / Iqbal, Azhar

Journal of pharmacy & bioallied sciences

2021 Volume 13, Issue Suppl 2, Page(s) S1084–S1087

Abstract: Aim: The aim of this study was to assess the effect of acidic beverages on color stability and microhardness of various esthetic restorative materials.: Materials and methods: A total of 60 samples were included in the present study. Group I: ... ...

Abstract	Aim: The aim of this study was to assess the effect of acidic beverages on color stability and microhardness of various esthetic restorative materials. Materials and methods: A total of 60 samples were included in the present study. Group I: Microhybrid composite resin, Group II: Nanoceramic, Group III: ORMOCER (Organically Modified Ceramics). 10 mm internal diameter and 5 mm depth of cylindrical aluminum mold of were utilized to prepare the samples. All the esthetic restorative samples were submerged for 10 min in 25 ml of acidic drink (Coca-Cola) each day up to 15 days. Vickers diamond intender was used to measure the microhardness of each sample. These values were calculated with baseline, 7 Results: The mean surface microhardness of 63.20 ± 0.46 was shown by Group III which was slightly more than that in Group II (60.08 ± 0.34) and Group I (58.10 ± 1.76). Analysis of covariance did not show any statistically significant difference between the groups. The samples in Group I showed the highest reduction in surface microhardness value after immersion into acidic drink, followed by Group II and Group III on 7 Conclusion: The present study concludes that the Organically Modified Ceramics esthetic restorative material showed the finest behavior after being dipped in the acidic drink followed next by Nanoceramic and Microhybrid composite resin.
Language	English
Publishing date	2021-11-10
Publishing country	India
Document type	Journal Article
ZDB-ID	2573569-X
ISSN	0975-7406 ; 0976-4879
ISSN (online)	0975-7406
ISSN	0976-4879
DOI	10.4103/jpbs.jpbs_189_21
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Article ; Online: Evaluation of Wettability of Three Saliva Substitutes on Heat-polymerized Acrylic Resin-An

Kumar, Pawan / Vadavadagi, Suneel V / Lahari, M / Shetty, Nitesh / Deb, Saikat / Dandekeri, Savita

The journal of contemporary dental practice

2019 Volume 20, Issue 5, Page(s) 557–560

Abstract: Aim: The aim of the present study was to evaluate the wettability of three saliva substitutes on heat-polymerized acrylic resin.: Materials and methods: Heat-cured acrylic resins 150 were made and divided into three groups containing 50 samples each, ...

Abstract	Aim: The aim of the present study was to evaluate the wettability of three saliva substitutes on heat-polymerized acrylic resin. Materials and methods: Heat-cured acrylic resins 150 were made and divided into three groups containing 50 samples each, i.e., group 1 Aqwet, group 2 Biotene, and group 3 Mouthkote. The prepared wax pattern was cut using 30 × 30-mm two square glass plates along the sides using a sharp carver. The uniformity of the wax samples was checked using the wax gauge. The wax samples were invested using dental plaster in varsity flasks. The samples were prepared using conventional heat-cure denture base acrylic resin. A goniometer was used to calculate receding and advancing contact angles for dynamic contact angle analysis. Results: The least mean value of advancing and receding contact angles was seen in group 1 Aqwet (68.12 ± 1.30 and 58.56 ± 0.10) followed by group 2 Biotene (81.64 ± 0.88 and 74.89 ± 0.45), and group 3 Mouthkote (85.76 ± 1.02 and 80.63 ± 0.66). A significant difference was found between the groups statistically with a Conclusion: The lowest advancing and receding contact angle values were significantly seen in the Aqwet saliva substitute followed by Biotene and Mouthkote on heat-polymerized acrylic resin. Clinical significance: The clinical significance of the saliva substitute's good wetting property on acrylic denture base. The quality of life of the patients with xerostomia can be improved using a suitable saliva substitute.
MeSH term(s)	Acrylic Resins ; Denture Bases ; Hot Temperature ; Humans ; Quality of Life ; Saliva ; Turkey ; Wettability
Chemical Substances	Acrylic Resins
Language	English
Publishing date	2019-05-01
Publishing country	India
Document type	Journal Article
ISSN	1526-3711
ISSN (online)	1526-3711
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: The Vaginal Microbiota Composition and Genital Infections during and after Pregnancy among Women in Pemba Island, Tanzania

Juliana, Naomi C. A. / Deb, Saikat / Juma, Mohamed H. / Poort, Linda / Budding, Andries E. / Mbarouk, Abdalla / Ali, Said M. / Ouburg, Sander / Morré, Servaas A. / Sazawal, Sunil / Ambrosino, Elena

Microorganisms. 2022 Feb. 25, v. 10, no. 3

2022

Abstract: We investigated the vaginal microbiota (VMB) composition, prevalence of genital pathogens and their association among pregnant and post-delivery women in Pemba Island, Tanzania. Vaginal swabs were collected from 90 women, at two time points during ... ...

Abstract	We investigated the vaginal microbiota (VMB) composition, prevalence of genital pathogens and their association among pregnant and post-delivery women in Pemba Island, Tanzania. Vaginal swabs were collected from 90 women, at two time points during pregnancy (<20 weeks of gestational age [GA] and ≥20 weeks GA) and once after delivery, when possible. IS-pro assay was used for VMB characterization. Chlamydia trachomatis (CT), Neisseria gonorrhea (NG), Trichomonas vaginalis (TV), Mycoplasma genitalium (MG) and human papillomavirus (HPV) were detected by qPCRs. VMB were mostly Lactobacillus dominant during pregnancy and non-Lactobacillus dominant post-delivery. A significant decrease in VMB richness was observed during pregnancy among paired and unpaired samples. Shannon diversity was significantly lower during pregnancy than post-delivery among unpaired samples. Klebsiella species and Streptococcus anginosus were the most commonly identified pathobionts at all timepoints. A high abundance of pathobionts was mostly seen in women with non-Lactobacillus dominant VMB. At ≥20 weeks GA timepoint during pregnancy, 63.0% of the women carrying one or more genital pathogen (either HPV, CT, TV, or MG) had L. iners dominant VMB. NG was not detected pre-delivery. This study contributes evidence on VMB composition, its changes during pregnancy and post-delivery, and their association with pathobionts and genital pathogens.
Keywords	Chlamydia trachomatis ; Klebsiella ; Lactobacillus ; Mycoplasma genitalium ; Neisseria ; Papillomaviridae ; Streptococcus anginosus ; Trichomonas vaginalis ; gestational age ; humans ; microorganisms ; pathogens ; pregnancy ; Tanzania
Language	English
Dates of publication	2022-0225
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms10030509
Database	NAL-Catalogue (AGRICOLA)

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Article: The Vaginal Microbiota Composition and Genital Infections during and after Pregnancy among Women in Pemba Island, Tanzania.

Juliana, Naomi C A / Deb, Saikat / Juma, Mohamed H / Poort, Linda / Budding, Andries E / Mbarouk, Abdalla / Ali, Said M / Ouburg, Sander / Morré, Servaas A / Sazawal, Sunil / Ambrosino, Elena

Microorganisms

2022 Volume 10, Issue 3

Abstract	We investigated the vaginal microbiota (VMB) composition, prevalence of genital pathogens and their association among pregnant and post-delivery women in Pemba Island, Tanzania. Vaginal swabs were collected from 90 women, at two time points during pregnancy (<20 weeks of gestational age [GA] and ≥20 weeks GA) and once after delivery, when possible. IS-pro assay was used for VMB characterization. Chlamydia trachomatis (CT), Neisseria gonorrhea (NG), Trichomonas vaginalis (TV), Mycoplasma genitalium (MG) and human papillomavirus (HPV) were detected by qPCRs. VMB were mostly Lactobacillus dominant during pregnancy and non-Lactobacillus dominant post-delivery. A significant decrease in VMB richness was observed during pregnancy among paired and unpaired samples. Shannon diversity was significantly lower during pregnancy than post-delivery among unpaired samples. Klebsiella species and Streptococcus anginosus were the most commonly identified pathobionts at all timepoints. A high abundance of pathobionts was mostly seen in women with non-Lactobacillus dominant VMB. At ≥20 weeks GA timepoint during pregnancy, 63.0% of the women carrying one or more genital pathogen (either HPV, CT, TV, or MG) had L. iners dominant VMB. NG was not detected pre-delivery. This study contributes evidence on VMB composition, its changes during pregnancy and post-delivery, and their association with pathobionts and genital pathogens.
Language	English
Publishing date	2022-02-25
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms10030509
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Article: The Prevalence of

Juliana, Naomi C A / Deb, Saikat / Ouburg, Sander / Chauhan, Aishwarya / Pleijster, Jolein / Ali, Said M / Morré, Servaas A / Sazawal, Sunil / Ambrosino, Elena

Pathogens (Basel, Switzerland)

2020 Volume 9, Issue 8

Abstract: Efforts to map the burden of infections globally have shown a high prevalence of genital infections, ... ...

Abstract	Efforts to map the burden of infections globally have shown a high prevalence of genital infections, including
Language	English
Publishing date	2020-07-31
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2695572-6
ISSN	2076-0817
ISSN	2076-0817
DOI	10.3390/pathogens9080625
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