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  1. Article ; Online: Protocol for microbiota analysis of a murine stroke model.

    Sorbie, Adam / Delgado Jiménez, Rosa / Weiler, Monica / Benakis, Corinne

    STAR protocols

    2023  Volume 4, Issue 1, Page(s) 101969

    Abstract: Investigations on the microbiota in neurological diseases such as stroke are increasingly common; however, stroke researchers may have limited experience with designing such studies. Here, we describe a protocol to conduct a stroke microbiota study in ... ...

    Abstract Investigations on the microbiota in neurological diseases such as stroke are increasingly common; however, stroke researchers may have limited experience with designing such studies. Here, we describe a protocol to conduct a stroke microbiota study in mice, from experimental stroke surgery and sample collection to data analysis. We provide details on sample processing and sequencing and provide a reproducible data analysis pipeline. In doing so, we hope to enable researchers to conduct robust studies and facilitate identification of stroke-associated microbial signatures. For complete details on the use and execution of this protocol, please refer to Sorbie et al. (2022).
    MeSH term(s) Animals ; Mice ; Stroke ; Data Analysis ; Microbiota
    Language English
    Publishing date 2023-01-09
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2666-1667
    ISSN (online) 2666-1667
    DOI 10.1016/j.xpro.2022.101969
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Increasing transparency and reproducibility in stroke-microbiota research: A toolbox for microbiota analysis.

    Sorbie, Adam / Delgado Jiménez, Rosa / Benakis, Corinne

    iScience

    2022  Volume 25, Issue 4, Page(s) 103998

    Abstract: Homeostasis of gut microbiota is crucial in maintaining human health. Alterations, or "dysbiosis," are increasingly implicated in human diseases, such as cancer, inflammatory bowel diseases, and, more recently, neurological disorders. In ischemic stroke ... ...

    Abstract Homeostasis of gut microbiota is crucial in maintaining human health. Alterations, or "dysbiosis," are increasingly implicated in human diseases, such as cancer, inflammatory bowel diseases, and, more recently, neurological disorders. In ischemic stroke patients, gut microbial profiles are markedly different compared to healthy controls, whereas manipulation of microbiota in animal models of stroke modulates outcome, further implicating microbiota in stroke pathobiology. Despite this, evidence for the involvement of specific microbes or microbial products and microbial signatures have yet to be identified, likely owing to differences in methodology, data analysis, and confounding variables between different studies. Here, we provide a set of guidelines to enable researchers to conduct high-quality, reproducible, and transparent microbiota studies, focusing on 16S rRNA sequencing in the emerging subfield of the stroke-microbiota. In doing so, we aim to facilitate novel and reproducible associations between the microbiota and brain diseases, including stroke, and translation into clinical practice.
    Language English
    Publishing date 2022-02-26
    Publishing country United States
    Document type Journal Article
    ISSN 2589-0042
    ISSN (online) 2589-0042
    DOI 10.1016/j.isci.2022.103998
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: The Gut Ecosystem: A Critical Player in Stroke.

    Delgado Jiménez, Rosa / Benakis, Corinne

    Neuromolecular medicine

    2020  Volume 23, Issue 2, Page(s) 236–241

    Abstract: The intestinal microbiome is emerging as a critical factor in health and disease. The microbes, although spatially restricted to the gut, are communicating and modulating the function of distant organs such as the brain. Stroke and other neurological ... ...

    Abstract The intestinal microbiome is emerging as a critical factor in health and disease. The microbes, although spatially restricted to the gut, are communicating and modulating the function of distant organs such as the brain. Stroke and other neurological disorders are associated with a disrupted microbiota. In turn, stroke-induced dysbiosis has a major impact on the disease outcome by modulating the immune response. In this review, we present current knowledge on the role of the gut microbiome in stroke, one of the most devastating brain disorders worldwide with very limited therapeutic options, and we discuss novel insights into the gut-immune-brain axis after an ischemic insult. Understanding the nature of the gut bacteria-brain crosstalk may lead to microbiome-based therapeutic approaches that can improve patient recovery.
    MeSH term(s) Aging ; Animals ; Anti-Bacterial Agents/therapeutic use ; Bacterial Translocation ; Brain Damage, Chronic/etiology ; Brain Damage, Chronic/prevention & control ; Brain Ischemia/etiology ; Brain Ischemia/microbiology ; Brain Ischemia/therapy ; Brain-Gut Axis/drug effects ; Dendritic Cells/immunology ; Disease Models, Animal ; Dysbiosis/complications ; Dysbiosis/immunology ; Dysbiosis/physiopathology ; Fatty Acids, Volatile/metabolism ; Fermentation ; Gastrointestinal Microbiome/drug effects ; Gastrointestinal Motility ; Humans ; Infarction/pathology ; Inflammation ; Intestines/blood supply ; Mice ; Norepinephrine/metabolism ; Stroke/etiology ; Stroke/microbiology ; Stroke/therapy ; T-Lymphocytes, Regulatory/immunology
    Chemical Substances Anti-Bacterial Agents ; Fatty Acids, Volatile ; Norepinephrine (X4W3ENH1CV)
    Language English
    Publishing date 2020-11-18
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2077809-0
    ISSN 1559-1174 ; 1535-1084
    ISSN (online) 1559-1174
    ISSN 1535-1084
    DOI 10.1007/s12017-020-08633-z
    Database MEDical Literature Analysis and Retrieval System OnLINE

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