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  1. Article ; Online: DietSee: An on-hand, portable, strip-type biosensor for lipolysis monitoring via real-time amperometric determination of glycerol in blood.

    Degrelle, Séverine A / Delile, Sébastien / Moog, Sophie / Mouisel, Etienne / O'Gorman, Donal / Moro, Cédric / Denechaud, Pierre-Damien / Torre, Cyril

    Analytica chimica acta

    2021  Volume 1155, Page(s) 338358

    Abstract: Glycerol is a clinical biomarker of lipolysis that is mainly produced by adipose tissues. Blood glycerol content increases in pathological conditions such as metabolic and cardiovascular diseases or cancer cachexia, but also in response to energetic ... ...

    Abstract Glycerol is a clinical biomarker of lipolysis that is mainly produced by adipose tissues. Blood glycerol content increases in pathological conditions such as metabolic and cardiovascular diseases or cancer cachexia, but also in response to energetic stress such as physical exercise. Accurate glycerol monitoring is therefore important in a range of healthcare contexts. However, current methods available for the quantification of glycerol are expensive, time-consuming, and require the extraction of plasma from blood, from which blood glycerol content is then extrapolated. Here, we report the development of a new point-of-care glycerometer device, DietSee, based on a strip-type biosensor that enables the quantification of glycerol directly from whole blood in 6 s. The performance of the biosensor was first evaluated using buffer solutions and spiked human and mouse plasma samples, and its response was compared with that of the gold-standard colorimetric method. The results obtained using DietSee correlated strongly with those from the reference method and demonstrated a linear response to glycerol levels across a wide range of concentrations (40-750 μM) that were representative of those in the human body. Next, the biosensor was validated using spiked human blood samples over a range of 30-55% hematocrit; it also demonstrated a strong correlation with reference measurements under these conditions (R
    MeSH term(s) Animals ; Biosensing Techniques ; Colorimetry ; Glycerol ; Lipolysis ; Mice
    Chemical Substances Glycerol (PDC6A3C0OX)
    Language English
    Publishing date 2021-02-27
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1483436-4
    ISSN 1873-4324 ; 0003-2670
    ISSN (online) 1873-4324
    ISSN 0003-2670
    DOI 10.1016/j.aca.2021.338358
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Electrochemical detection of nitromethane vapors combined with a solubilization device

    Delile, Sébastien / Adeline Aussage / Michel Cassir / Pascal Palmas / Thierry Maillou / Virginie Lair

    Talanta. 2015 Jan. 15, v. 132

    2015  

    Abstract: During the past decade, the number of terrorism acts has increased and the need for efficient explosive detectors has become an urgent worldwide necessity. A prototype, NebulexTM, was recently developed in our laboratory. Basically, it couples the ... ...

    Abstract During the past decade, the number of terrorism acts has increased and the need for efficient explosive detectors has become an urgent worldwide necessity. A prototype, NebulexTM, was recently developed in our laboratory. Basically, it couples the solubilization of an analyte from the atmosphere by a nebulization process and in-situ detection. This article presents the development and integration of an electrochemical sensor for the detection of nitromethane, a common chemical product that can be used to make an improvised explosive device. A gold screen-printed electrode was used in a flow-cell and a detection limit of 4.5μM was achieved by square wave voltammetry. The detection method was also determined to be selective toward nitromethane over a large panel of interfering compounds. Detection tests with the NebulexTM were thus carried out using a custom-made calibrated nitromethane vapor generator. Detection times of less than one minute were obtained for nitromethane contents of 8 and 90ppmv. Further measurements were performed in a room-measurement configuration leading to detection times in the range of 1–2min, clearly demonstrating the system׳s efficiency under quasi-real conditions.
    Keywords atomization ; electrochemistry ; electrodes ; gold ; prototypes ; sensors (equipment) ; solubilization ; terrorism ; vapors
    Language English
    Dates of publication 2015-0115
    Size p. 334-338.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 1500969-5
    ISSN 1873-3573 ; 0039-9140
    ISSN (online) 1873-3573
    ISSN 0039-9140
    DOI 10.1016/j.talanta.2014.09.024
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: Electrochemical Sensors Based on Screen-Printed Electrodes: The Use of Phthalocyanine Derivatives for Application in VFA Detection.

    Ndiaye, Amadou L / Delile, Sébastien / Brunet, Jérôme / Varenne, Christelle / Pauly, Alain

    Biosensors

    2016  Volume 6, Issue 3

    Abstract: Here, we report on the use of electrochemical methods for the detection of volatiles fatty acids (VFAs), namely acetic acid. We used tetra-tert-butyl phthalocyanine (PcH₂-tBu) as the sensing material and investigated its electroanalytical properties by ... ...

    Abstract Here, we report on the use of electrochemical methods for the detection of volatiles fatty acids (VFAs), namely acetic acid. We used tetra-tert-butyl phthalocyanine (PcH₂-tBu) as the sensing material and investigated its electroanalytical properties by means of cyclic voltammetry (CV) and square wave voltammetry (SWV). To realize the electrochemical sensing system, the PcH₂-tBu has been dropcast-deposited on carbon (C) orgold (Au)screen-printed electrodes (SPEs) and characterized by cyclic voltammetry and scanning electron microscopy (SEM). The SEM analysis reveals that the PcH₂-tBu forms mainly aggregates on the SPEs. The modified electrodes are used for the detection of acetic acid and present a linear current increase when the acetic acid concentration increases. The Cmodified electrode presents a limit of detection (LOD) of 25.77 mM in the range of 100 mM-400 mM, while the Aumodified electrode presents an LOD averaging 40.89 mM in the range of 50 mM-300 mM. When the experiment is realized in a buffered condition, theCmodified electrode presents a lower LOD, which averagesthe 7.76 mM. A pronounced signal decay attributed to an electrode alteration is observed in the case of the gold electrode. This electrode alteration severely affects the coating stability. This alteration is less perceptible in the case of the carbon electrode.
    MeSH term(s) Acetic Acid/analysis ; Biosensing Techniques ; Electrochemical Techniques ; Electrodes ; Indoles/analysis ; Limit of Detection
    Chemical Substances Indoles ; Acetic Acid (Q40Q9N063P) ; phthalocyanine (V5PUF4VLGY)
    Language English
    Publishing date 2016-09-01
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662125-3
    ISSN 2079-6374 ; 2079-6374
    ISSN (online) 2079-6374
    ISSN 2079-6374
    DOI 10.3390/bios6030046
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Interaction of osmium(ii) redox probes with DNA: insights from theory.

    Sharma, Ashwani / Delile, Sebastien / Jabri, Mohamed / Adamo, Carlo / Fave, Claire / Marchal, Damien / Perrier, Aurélie

    Physical chemistry chemical physics : PCCP

    2016  Volume 18, Issue 43, Page(s) 30029–30039

    Abstract: In the course of developing ultrasensitive and quantitative electrochemical point-of-care analytical tools for genetic detection of infectious diseases, osmium(ii) metallointercalators were revealed to be suitable and efficient redox probes to monitor ... ...

    Abstract In the course of developing ultrasensitive and quantitative electrochemical point-of-care analytical tools for genetic detection of infectious diseases, osmium(ii) metallointercalators were revealed to be suitable and efficient redox probes to monitor the in vitro DNA amplification [Defever etal, Anal. Chem., 2011, 83, 1815-1821]. In this work, we thus propose a complete computational protocol in order to evaluate the affinity between Os(ii) complexes with double-stranded DNA. This protocol is based on molecular dynamics, with the parametrization of the GAFF force field for the Os(ii) complexes presenting an octahedral environment with polypyridine ligands, and QM/QM' calculations to evaluate the binding energy. For three Os(ii) probes and different binding sites, molecular dynamics simulations and interaction energies calculated at the QM/QM' level are successively discussed and compared to experimental data in order to identify the most stable binding sites. The computational protocol we propose should then be used to design more efficient Os(ii) metallointercalators.
    Language English
    Publishing date 2016-11-02
    Publishing country England
    Document type Journal Article
    ZDB-ID 1476244-4
    ISSN 1463-9084 ; 1463-9076
    ISSN (online) 1463-9084
    ISSN 1463-9076
    DOI 10.1039/c6cp05105g
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Electrochemical detection of nitromethane vapors combined with a solubilization device.

    Delile, Sébastien / Aussage, Adeline / Maillou, Thierry / Palmas, Pascal / Lair, Virginie / Cassir, Michel

    Talanta

    2015  Volume 132, Page(s) 334–338

    Abstract: During the past decade, the number of terrorism acts has increased and the need for efficient explosive detectors has become an urgent worldwide necessity. A prototype, Nebulex™, was recently developed in our laboratory. Basically, it couples the ... ...

    Abstract During the past decade, the number of terrorism acts has increased and the need for efficient explosive detectors has become an urgent worldwide necessity. A prototype, Nebulex™, was recently developed in our laboratory. Basically, it couples the solubilization of an analyte from the atmosphere by a nebulization process and in-situ detection. This article presents the development and integration of an electrochemical sensor for the detection of nitromethane, a common chemical product that can be used to make an improvised explosive device. A gold screen-printed electrode was used in a flow-cell and a detection limit of 4.5 µM was achieved by square wave voltammetry. The detection method was also determined to be selective toward nitromethane over a large panel of interfering compounds. Detection tests with the Nebulex™ were thus carried out using a custom-made calibrated nitromethane vapor generator. Detection times of less than one minute were obtained for nitromethane contents of 8 and 90 ppmv. Further measurements were performed in a room-measurement configuration leading to detection times in the range of 1-2 min, clearly demonstrating the system's efficiency under quasi-real conditions.
    MeSH term(s) Calibration ; Carbon ; Electrochemical Techniques ; Electrodes ; Explosive Agents/analysis ; Gold/chemistry ; Humans ; Limit of Detection ; Methane/analogs & derivatives ; Methane/analysis ; Nitroparaffins/analysis ; Terrorism/prevention & control ; Time Factors
    Chemical Substances Explosive Agents ; Nitroparaffins ; Carbon (7440-44-0) ; Gold (7440-57-5) ; Methane (OP0UW79H66) ; nitromethane (RU5WG8C3F4)
    Language English
    Publishing date 2015-01
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1500969-5
    ISSN 1873-3573 ; 0039-9140
    ISSN (online) 1873-3573
    ISSN 0039-9140
    DOI 10.1016/j.talanta.2014.09.024
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

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