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Article ; Online: Villosol reverses 5-FU resistance in colorectal cancer by inhibiting the CDKN2A gene regulated TP53-PI3K/Akt signaling axis.

Chen, Han / Deng, Jiao / Hou, Tie-Wei / Shan, Yong-Qi

2024 Volume 325, Page(s) 117907

Abstract: Ethnopharmacological relevance: Patrinia villosa (Juss.) (PV) is the drug of choice in traditional Chinese medicine for the treatment of colorectal cancer (CRC) and has achieved reliable efficacy in clinic. Villosol is the active ingredient in PV. ... ...

Abstract	Ethnopharmacological relevance: Patrinia villosa (Juss.) (PV) is the drug of choice in traditional Chinese medicine for the treatment of colorectal cancer (CRC) and has achieved reliable efficacy in clinic. Villosol is the active ingredient in PV. However, the molecular mechanism by which Villosol reverses chemoresistance in CRC remains unclear. Aim of the study: Analysis of the molecular mechanism by which Villosol, the active ingredient of PV, reverses CRC/5-FU resistance through modulation of the CDKN2A gene was validated by network pharmacology techniques and experiments. Materials and methods: We identified CDKN2A as a gene associated with 5-FU resistance through gene chip analysis. Next, we conducted a series of functional analyses in cell lines, animal samples, and xenograft models to investigate the role, clinical significance, and abnormal regulatory mechanisms of CDKN2A in 5-FU resistance in CRC. In addition, we screened and obtained a raw ingredient called Villosol, which targets CDKN2A, and investigated its pharmacological effects. Results: Analysis of CRC cells and animal samples showed that the upregulation of CDKN2A expression was strongly associated with 5-FU resistance. CRC cells overexpressing CDKN2A showed reduced sensitivity to 5-FU and enhanced tumor biology in vitro. Inhibition of aberrant activation of CDKN2A enhances the expression of TP53. Mechanistically, overexpression of CDKN2A activates the PI3K/Akt pathway and induces resistance to 5-FU. Villosol inhibited CDKN2A, and CRC/5-FU cells regained sensitivity to 5-FU. Villosol effectively reverses 5-FU resistance through the CDKN2A-TP53-PI3K/Akt axis. Conclusion: Changes in CDKN2A gene expression can be used to predict the response of CRC patients to 5-FU therapy. Additionally, inhibiting CDKN2A activation with Villosol may present a new approach to overcoming 5-FU resistance in clinical settings.
MeSH term(s)	Animals ; Humans ; Proto-Oncogene Proteins c-akt/metabolism ; Phosphatidylinositol 3-Kinases/metabolism ; Fluorouracil/pharmacology ; Fluorouracil/therapeutic use ; Genes, p16 ; Cell Line, Tumor ; Apoptosis ; Xenograft Model Antitumor Assays ; Colorectal Neoplasms/drug therapy ; Colorectal Neoplasms/genetics ; Colorectal Neoplasms/pathology ; Drug Resistance, Neoplasm ; Tumor Suppressor Protein p53/genetics ; Cyclin-Dependent Kinase Inhibitor p16/genetics ; Cyclin-Dependent Kinase Inhibitor p16/metabolism ; Cyclin-Dependent Kinase Inhibitor p16/pharmacology ; Lactones
Chemical Substances	Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; villosol (60077-62-5) ; Fluorouracil (U3P01618RT) ; TP53 protein, human ; Tumor Suppressor Protein p53 ; CDKN2A protein, human ; Cyclin-Dependent Kinase Inhibitor p16 ; Lactones
Language	English
Publishing date	2024-02-10
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2024.117907
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Article ; Online: Transcutaneous electric acupoint stimulation reduced consumption of profopol in patients undergoing laparoscopic surgery: A randomized clinical trial.

Zhang, Minjuan / Cairen, Zhuoma / Liu, Xiaoyu / Deng, Jiao / Mu, Xiaoxiao / Wang, Yun / Lu, Zhihong

Medicine

2024 Volume 103, Issue 4, Page(s) e35730

Abstract: Background: Propofol is a common regimen for general anesthesia maintenance. But propofol can dose-dependently generate cardiopulmonary depression. Thus, any strategy to reduce propofol dosage during laparoscopic surgery may have underlying beneficial ... ...

Abstract	Background: Propofol is a common regimen for general anesthesia maintenance. But propofol can dose-dependently generate cardiopulmonary depression. Thus, any strategy to reduce propofol dosage during laparoscopic surgery may have underlying beneficial effect for patient prognosis. Previous studies indicated both analgesic and sedative effect of acupoint stimulation. However, its main sedative effect on patients under general anesthesia remains unclear. Objective: The aim of this study was to investigate the sedative effect of transcutaneous electrical acupoint stimulation (TEAS) on patients scheduled for laparoscopic surgery under general anesthesia. Design, setting, participants and interventions: In this randomized clinical trial, patients scheduled for laparoscopic surgery under general anesthesia in Xijing hospital were randomly assigned to 3 groups, receiving electrical stimulation at the Shenmen (HT7)/Ximen (PC4) (TEAS group), stimulation at the shoulder (non-acupoint group) or no stimulation (control group), respectively. Main outcome measures: One hundred sixty-two patients completed the study. The primary outcome was the consumption of propofol, and secondary outcomes included features of recovery after surgery, major complications after surgery and by 1 year after surgery. Results: In patients undergoing laparoscopic surgery, the doses of propofol decreased significantly in the TEAS group compared (0.10 ± 0.02 mg·kg-1·min-1) with the other 2 groups (both 0.12 ± 0.02 mg·kg-1·min-1, P < .001). The mean differences (95% confidence interval) for non-acupoint versus TEAS and control versus TEAS were 0.021 (0.012, 0.030) and 0.024 (0.013, 0.034), respectively. Time to awake and to extubation were not significantly different among the groups. The incidences of major complications after surgery and by 1 year after surgery were not significantly different among the groups. Conclusion: TEAS could induce additional sedative effect in patients during laparoscopic surgery and reduce propofol consumption.
MeSH term(s)	Humans ; Propofol ; Acupuncture Points ; Transcutaneous Electric Nerve Stimulation ; Laparoscopy ; Hypnotics and Sedatives
Chemical Substances	Propofol (YI7VU623SF) ; Hypnotics and Sedatives
Language	English
Publishing date	2024-01-26
Publishing country	United States
Document type	Randomized Controlled Trial ; Journal Article
ZDB-ID	80184-7
ISSN	1536-5964 ; 0025-7974
ISSN (online)	1536-5964
ISSN	0025-7974
DOI	10.1097/MD.0000000000035730
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Article ; Online: Revolutionizing aquatic eco-environmental monitoring: Utilizing the RPA-Cas-FQ detection platform for zooplankton.

Hu, Huan / Liu, Li / Wei, Xing-Yi / Duan, Jin-Jing / Deng, Jiao-Yun / Pei, De-Sheng

The Science of the total environment

2024 Volume 929, Page(s) 172414

Abstract: The integration of recombinase polymerase amplification (RPA) with CRISPR/Cas technology has revolutionized molecular diagnostics and pathogen detection due to its unparalleled sensitivity and trans-cleavage ability. However, its potential in the ... ...

Abstract	The integration of recombinase polymerase amplification (RPA) with CRISPR/Cas technology has revolutionized molecular diagnostics and pathogen detection due to its unparalleled sensitivity and trans-cleavage ability. However, its potential in the ecological and environmental monitoring scenarios for aquatic ecosystems remains largely unexplored, particularly in accurate qualitative/quantitative detection, and its actual performance in handling complex real environmental samples. Using zooplankton as a model, we have successfully optimized the RPA-CRISPR/Cas12a fluorescence detection platform (RPA-Cas-FQ), providing several crucial "technical tips". Our findings indicate the sensitivity of CRISPR/Cas12a alone is 5 × 10
Language	English
Publishing date	2024-04-15
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	121506-1
ISSN	1879-1026 ; 0048-9697
ISSN (online)	1879-1026
ISSN	0048-9697
DOI	10.1016/j.scitotenv.2024.172414
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Article: The high-quality genome of lotus reveals tandem duplicate genes involved in stress response and secondary metabolites biosynthesis.

Qi, Huanhuan / Yu, Feng / Deng, Jiao / Zhang, Liangsheng / Yang, Pingfang

Horticulture research

2023 Volume 10, Issue 5, Page(s) uhad040

Language	English
Publishing date	2023-02-28
Publishing country	England
Document type	Journal Article
ISSN	2662-6810
ISSN	2662-6810
DOI	10.1093/hr/uhad040
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Article ; Online: Studies on Lotus Genomics and the Contribution to Its Breeding.

Qi, Huanhuan / Yu, Feng / Deng, Jiao / Yang, Pingfang

International journal of molecular sciences

2022 Volume 23, Issue 13

Abstract: Lotus ( ...

Abstract	Lotus (
MeSH term(s)	Genome, Plant ; Genomics ; Lotus/genetics ; Nelumbo/genetics ; Plant Breeding
Language	English
Publishing date	2022-06-30
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms23137270
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Article: Morphological and molecular evidence gives insight into the taxonomic position of

Deng, Jiao-Jiao / Liu, Chang-Kun / Zhou, Song-Dong / He, Xing-Jin

PhytoKeys

2022 Volume 213, Page(s) 19–33

Abstract: In this study, morphological and molecular evidences were combined to determine the taxonomic position ... ...

Abstract	In this study, morphological and molecular evidences were combined to determine the taxonomic position of
Language	English
Publishing date	2022-11-09
Publishing country	Bulgaria
Document type	Journal Article
ZDB-ID	2579891-1
ISSN	1314-2003 ; 1314-2011
ISSN (online)	1314-2003
ISSN	1314-2011
DOI	10.3897/phytokeys.213.89784
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Article ; Online: The complete plastomes of thirteen Libanotis (Apiaceae, Apioideae) plants: comparative and phylogenetic analyses provide insights into the plastome evolution and taxonomy of Libanotis.

Liu, Li-Jia / Liu, Chang-Kun / Cai, Jing / Deng, Jiao-Jiao / He, Xing-Jin / Zhou, Song-Dong

BMC plant biology

2024 Volume 24, Issue 1, Page(s) 106

Abstract: Background: The genus Libanotis Haller ex Zinn, nom. cons., a contentious member of Apiaceae, encompasses numerous economically and medicinally significant plants, comprising approximately 30 species distributed across Eurasia. Despite many previous ... ...

Abstract	Background: The genus Libanotis Haller ex Zinn, nom. cons., a contentious member of Apiaceae, encompasses numerous economically and medicinally significant plants, comprising approximately 30 species distributed across Eurasia. Despite many previous taxonomic insights into it, phylogenetic studies of the genus are still lacking. And the establishment of a robust phylogenetic framework remains elusive, impeding advancements and revisions in the taxonomic system for this genus. Plastomes with greater variability in their genetic characteristics hold promise for building a more robust Libanotis phylogeny. Results: During our research, we sequenced, assembled, and annotated complete plastomes for twelve Libanotis species belong to three sections and two closely related taxa. We conducted a comprehensive comparative analysis through totally thirteen Libanotis plastomes for the genus, including an additional plastome that had been published. Our results suggested that Libanotis plastome was highly conserved between different subclades, while the coding regions were more conserved than the non-coding regions, and the IR regions were more conserved than the single copy regions. Nevertheless, eight mutation hotspot regions were identified among plastomes, which can be considered as candidate DNA barcodes for accurate species identification in Libanotis. The phylogenetic analyses generated a robustly framework for Libanotis and revealed that Libanotis was not a monophyletic group and their all three sections were polygenetic. Libanotis schrenkiana was sister to L. sibirica, type species of this genus, but the remainders scattered within Selineae. Conclusion: The plastomes of Libanotis exhibited a high degree of conservation and was effective in enhancing the support and resolution of phylogenetic analyses within this genus. Based on evidence from both phylogeny and morphology, we propose the recognition of "Libanotis sensu stricto" and provide taxonomic recommendations for other taxa that previously belonged to Libanotis. In conclusion, our study not only revealed the phylogenetic position and plastid evolution of Libanotis, but also provided new insights into the phylogeny of the family Apiaceae and phylogenetic relationships within the tribe Selineae.
MeSH term(s)	Phylogeny ; Apiaceae ; Evolution, Molecular ; Plastids/genetics ; Plants
Language	English
Publishing date	2024-02-12
Publishing country	England
Document type	Journal Article
ZDB-ID	2059868-3
ISSN	1471-2229 ; 1471-2229
ISSN (online)	1471-2229
ISSN	1471-2229
DOI	10.1186/s12870-024-04784-4
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Article ; Online: SPL16 and SPL23 mediate photoperiodic control of seasonal growth in Populus trees

Wei, Hongbin / Luo, Mengting / Deng, Jiao / Xiao, Yue / Yan, Huiting / Liu, Huajie / Li, Yi / Song, Qin / Xiao, Xingyue / Shen, Junlong / Kong, Hanying / Sun, Fan / Luo, Keming

New Phytologist. 2024 Feb., v. 241, no. 4 p.1646-1661

2024

Abstract: Perennial trees in boreal and temperate regions undergo growth cessation and bud set under short photoperiods, which are regulated by phytochrome B (phyB) photoreceptors and PHYTOCHROME INTERACTING FACTOR 8 (PIF8) proteins. However, the direct signaling ... ...

Abstract	Perennial trees in boreal and temperate regions undergo growth cessation and bud set under short photoperiods, which are regulated by phytochrome B (phyB) photoreceptors and PHYTOCHROME INTERACTING FACTOR 8 (PIF8) proteins. However, the direct signaling components downstream of the phyB‐PIF8 module remain unclear. We found that short photoperiods suppressed the expression of miR156, while upregulated the expression of miR156‐targeted SQUAMOSA‐PROMOTER BINDING PROTEIN‐LIKE 16 (SPL16) and SPL23 in leaves and shoot apices of Populus trees. Accordingly, either overexpression of MIR156a/c or mutagenesis of SPL16/23 resulted in the attenuation of growth cessation and bud set under short days (SD), whereas overexpression of SPL16 and SPL23 conferred early growth cessation. We further showed that SPL16 and SPL23 directly suppressed FLOWERING LOCUS T2 (FT2) expression while promoted BRANCHED1 (BRC1.1 and BRC1.2) expression. Moreover, we revealed that PIF8.1/8.2, positive regulators of growth cessation, directly bound to promoters of MIR156a and MIR156c and inhibited their expression to modulate downstream pathways. Our results reveal a connection between the phyB‐PIF8 module‐mediated photoperiod perception and the miR156‐SPL16/23‐FT2/BRC1 regulatory cascades in SD‐induced growth cessation. Our study provides insights into the rewiring of a conserved miR156‐SPL module in the regulation of seasonal growth in Populus trees.
Keywords	Populus ; bud set ; loci ; mutagenesis ; phytochrome ; seasonal growth
Language	English
Dates of publication	2024-02
Size	p. 1646-1661.
Publishing place	John Wiley & Sons, Ltd
Document type	Article ; Online
Note	JOURNAL ARTICLE
ZDB-ID	208885-x
ISSN	1469-8137 ; 0028-646X
ISSN (online)	1469-8137
ISSN	0028-646X
DOI	10.1111/nph.19485
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: QTL mapping and candidate gene analysis for yield and grain weight/size in Tartary buckwheat

Li, Ruiyuan / Chen, Zhengfeng / Zheng, Ran / Chen, Qingfu / Deng, Jiao / Li, Hongyou / Huang, Juan / Liang, Chenggang / Shi, Taoxiong

BMC Plant Biol. 2023 Dec., v. 23, no. 1 p.58-58

2023

Abstract: BACKGROUND: Grain weight/size influences not only grain yield (GY) but also nutritional and appearance quality and consumer preference in Tartary buckwheat. The identification of quantitative trait loci (QTLs)/genes for grain weight/size is an important ... ...

Abstract	BACKGROUND: Grain weight/size influences not only grain yield (GY) but also nutritional and appearance quality and consumer preference in Tartary buckwheat. The identification of quantitative trait loci (QTLs)/genes for grain weight/size is an important objective of Tartary buckwheat genetic research and breeding programs. RESULTS: Herein, we mapped the QTLs for GY, 1000-grain weight (TGW), grain length (GL), grain width (GW) and grain length–width ratio (L/W) in four environments using 221 recombinant inbred lines (XJ-RILs) derived from a cross of 'Xiaomiqiao × Jinqiaomai 2'. In total, 32 QTLs, including 7 for GY, 5 for TGW, 6 for GL, 11 for GW and 3 for L/W, were detected and distributed in 24 genomic regions. Two QTL clusters, qClu-1-3 and qClu-1-5, located on chromosome Ft1, were revealed to harbour 7 stable major QTLs for GY (qGY1.2), TGW (qTGW1.2), GL (qGL1.1 and qGL1.4), GW (qGW1.7 and qGW1.10) and L/W (qL/W1.2) repeatedly detected in three and above environments. A total of 59 homologues of 27 known plant grain weight/size genes were found within the physical intervals of qClu-1-3 and qClu-1-5. Six homologues, FtBRI1, FtAGB1, FtTGW6, FtMADS1, FtMKK4 and FtANT, were identified with both non-synonymous SNP/InDel variations and significantly differential expression levels between the two parents, which may play important roles in Tatary buckwheat grain weight/size control and were chosen as core candidate genes for further investigation. CONCLUSIONS: Two stable major QTL clusters related to grain weight/size and six potential key candidate genes were identified by homology comparison, SNP/InDel variations and qRT‒qPCR analysis between the two parents. Our research provides valuable information for improving grain weight/size and yield in Tartary buckwheat breeding.
Keywords	buckwheat ; chromosomes ; consumer preferences ; gene expression regulation ; genes ; genomics ; grain yield ; quantitative traits
Language	English
Dates of publication	2023-12
Size	p. 58.
Publishing place	BioMed Central
Document type	Article ; Online
ZDB-ID	2059868-3
ISSN	1471-2229
ISSN	1471-2229
DOI	10.1186/s12870-022-04004-x
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Article ; Online: Proteomic and metabolomic analyses showing the differentially accumulation of NnUFGT2 is involved in the petal red-white bicolor pigmentation in lotus (Nelumbo nucifera)

Deng, Jiao / Su, Mengyue / Zhang, Xinyi / Liu, Xuelian / Damaris, Rebecca Njeri / Lv, Shiyou / Yang, Pingfang

Plant Physiology and Biochemistry. 2023 May, v. 198 p.107675-

2023

Abstract: Bicolor flower lotus is rare with high ornamental value. During the long history of breeding and artificial selection, a very famous lotus cultivar 'Da Sajin' with red and white picotee bicolor petals were obtained. In order to reveal the mechanism ... ...

Abstract	Bicolor flower lotus is rare with high ornamental value. During the long history of breeding and artificial selection, a very famous lotus cultivar 'Da Sajin' with red and white picotee bicolor petals were obtained. In order to reveal the mechanism underlying the formation of its picotee bicolor pattern in the petal, an integrative metabolomics and proteomics analyses were conducted between red and white parts of its petals. The results showed that the defect of anthocyanidin 3-O-glucosyltransferases (UFGTs) accumulation resulted in the failure of the glycosylation of anthocyanidin, the last step of anthocyanin biosynthesis in white part of the petals. And proteomic data and biochemical analysis showed that the defect of UFGTs accumulation is not related to their transcription, but because of their degradation. Function of one differentially accumulated NnUFGT were proven being involved in anthocyanin biosynthesis through both in-vitro enzyme assay and in-vivo transgenic analyses. This regulation on the protein accumulation of structural genes in anthocyanin biosynthesis was not explored in any other plants, and hence supposed to be a novel mechanism for the formation of picotee bicolor pattern flower. The results not only provide some new insights into the understanding of lotus flower coloration, but also might assist the breeding of flower lotus.
Keywords	Nelumbo nucifera ; anthocyanidin 3-O-glucosyltransferase ; anthocyanidins ; anthocyanins ; artificial selection ; biosynthesis ; color ; cultivars ; flowers ; genetically modified organisms ; glycosylation ; metabolomics ; ornamental value ; pigmentation ; plant physiology ; proteomics ; Bicolor flower ; Anthocyanin ; NnUFGT
Language	English
Dates of publication	2023-05
Publishing place	Elsevier Masson SAS
Document type	Article ; Online
ZDB-ID	742978-2
ISSN	1873-2690 ; 0981-9428
ISSN (online)	1873-2690
ISSN	0981-9428
DOI	10.1016/j.plaphy.2023.107675
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