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  1. Article: Isolation and cultivation as well as

    Heilen, Laura Beate / Roßgardt, Jessica / Dern-Wieloch, Jutta / Vogelsberg, Jörg / Staszyk, Carsten

    Frontiers in veterinary science

    2023  Volume 10, Page(s) 1116671

    Abstract: Introduction: The lifelong eruption places a great demand on the dental pulp and periodontal ligament (PDL) of horse teeth. Cells within the pulp and PDL seem to play a key role during this remodeling.: Methods: In this study, we isolated and ... ...

    Abstract Introduction: The lifelong eruption places a great demand on the dental pulp and periodontal ligament (PDL) of horse teeth. Cells within the pulp and PDL seem to play a key role during this remodeling.
    Methods: In this study, we isolated and cultivated MSCs (medicinal signaling cells) from dental pulp, PDL and retrobulbar fat of four horses. Subsequently, we analyzed them by flow cytometry and immunohistochemistry to determine and compare their characteristics. In addition, we localized these cells within the tissue structure
    Results: The described method illustrates a feasible approach to isolate and cultivate MSCs from equine dental pulp and PDL. In the flow cytometry a vast majority of cultivated cells were positive for CD90 and CD40 and negative for CD11a/18, CD45, CD105 and MHCII suggesting that these cells feature characteristics of MSCs. Immunohistochemistry of histological pulp and PDL sections showed the localization of CD90 positive cells especially in the perivascular region and the subodontoblastic layer.
    Discussion: Our findings indicate that the isolation and cultivation of MSCs from equine dental pulp and PDL is feasible although an elaborate and complicated harvesting protocol is required. MSCs isolated from dental pulp and PDL are regarded as candidates for new therapeutical approaches in equine dental medicine like regeneration of periodontal lesions, enhancement of periodontal re-attachment after dental replantation and stimulation of pulp-obliteration and apexification in combination with endodontic therapies.
    Language English
    Publishing date 2023-03-10
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2834243-4
    ISSN 2297-1769
    ISSN 2297-1769
    DOI 10.3389/fvets.2023.1116671
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: The Equine Dental Pulp: Histomorphometric Analysis of the Equine Dental Pulp in Incisors and Cheek Teeth

    Roßgardt, Jessica / Heilen, Laura Beate / Büttner, Kathrin / Dern-Wieloch, Jutta / Vogelsberg, Jörg / Staszyk, Carsten

    Veterinary sciences. 2022 May 30, v. 9, no. 6

    2022  

    Abstract: To maintain a healthy and functional status, equine hypsodont teeth have to produce lifelong large amounts of subocclusal dentin to prevent occlusal pulp exposure, which is caused by occlusal wear. To examine the cyto- and histological components that ... ...

    Abstract To maintain a healthy and functional status, equine hypsodont teeth have to produce lifelong large amounts of subocclusal dentin to prevent occlusal pulp exposure, which is caused by occlusal wear. To examine the cyto- and histological components that guarantee the lifelong high productivity of equine pulp, a limited number of ten incisors and ten cheek teeth from seven adult horses (aged 5 to 24 years) and five foals were sampled for preliminary histomorphometric and histomorphological evaluations. Independently of age, the equine dental pulp featured constant layers of predentin and odontoblastic cells, as well as soft connective tissue, composed of a cellular fibrous matrix, in which blood vessels and nerve fibers were embedded. As a result of the progressive deposition of newly formed dentin, the layer of dentin became thicker with age, and the size of the pulp chamber decreased. In contrast to the brachydont teeth, the morphological characteristics of the odontoblastic layer and the width of the predentin layer did not change with age. Therefore, it is assumed that the equine pulp tissue retained their juvenile status, which explains its unchanged ability to produce high amounts of subocclusal dentin. These preliminary, but clinically significant, findings are worthy of further investigation in order to identify strategies for equine-specific endodontic therapies.
    Keywords adults ; blood ; functional status ; histology ; juveniles ; nerve tissue ; pulp ; tooth pulp
    Language English
    Dates of publication 2022-0530
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2768971-2
    ISSN 2306-7381
    ISSN 2306-7381
    DOI 10.3390/vetsci9060261
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: The Equine Dental Pulp: Analysis of the Stratigraphic Arrangement of the Equine Dental Pulp in Incisors and Cheek Teeth.

    Roßgardt, Jessica / Heilen, Laura Beate / Büttner, Kathrin / Dern-Wieloch, Jutta / Vogelsberg, Jörg / Staszyk, Carsten

    Veterinary sciences

    2022  Volume 9, Issue 11

    Abstract: In the crown pulp of brachydont teeth, a cell-free and a cell-rich zone are established beneath the odontoblastic layer, indicating a mature status. For the equine dental pulp, there are no descriptions which allow for a comparative analysis with regard ... ...

    Abstract In the crown pulp of brachydont teeth, a cell-free and a cell-rich zone are established beneath the odontoblastic layer, indicating a mature status. For the equine dental pulp, there are no descriptions which allow for a comparative analysis with regard to functional requirements in terms of lifelong secondary dentin production to compensate for occlusal wear. For histomorphological and immunohistological investigations, ten incisors and ten check teeth were used from seven adult horses and five foals. In the periphery of the equine dental pulp, a constant predentin and odontoblastic cell layer was present, followed by densely packed fibroblastic cells, capillary networks, and a high concentration of nerve fibers, suggesting a subodontoblastic supportive zone. Whilst the size of the equine dental pulp decreased with age, the numbers of blood vessels, nerve fibers, and fibroblastic cells increased with age. Histological analysis of the equine dental pulp did not show a cell-free and cell-rich zone as described in the brachydont crown pulp. The equine dental pulp remained in a juvenile status even in aged horses, with morphological features indicating a high capacity for dentine production.
    Language English
    Publishing date 2022-10-30
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2768971-2
    ISSN 2306-7381 ; 2306-7381
    ISSN (online) 2306-7381
    ISSN 2306-7381
    DOI 10.3390/vetsci9110602
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: The Equine Dental Pulp: Histomorphometric Analysis of the Equine Dental Pulp in Incisors and Cheek Teeth.

    Roßgardt, Jessica / Heilen, Laura Beate / Büttner, Kathrin / Dern-Wieloch, Jutta / Vogelsberg, Jörg / Staszyk, Carsten

    Veterinary sciences

    2022  Volume 9, Issue 6

    Abstract: To maintain a healthy and functional status, equine hypsodont teeth have to produce lifelong large amounts of subocclusal dentin to prevent occlusal pulp exposure, which is caused by occlusal wear. To examine the cyto- and histological components that ... ...

    Abstract To maintain a healthy and functional status, equine hypsodont teeth have to produce lifelong large amounts of subocclusal dentin to prevent occlusal pulp exposure, which is caused by occlusal wear. To examine the cyto- and histological components that guarantee the lifelong high productivity of equine pulp, a limited number of ten incisors and ten cheek teeth from seven adult horses (aged 5 to 24 years) and five foals were sampled for preliminary histomorphometric and histomorphological evaluations. Independently of age, the equine dental pulp featured constant layers of predentin and odontoblastic cells, as well as soft connective tissue, composed of a cellular fibrous matrix, in which blood vessels and nerve fibers were embedded. As a result of the progressive deposition of newly formed dentin, the layer of dentin became thicker with age, and the size of the pulp chamber decreased. In contrast to the brachydont teeth, the morphological characteristics of the odontoblastic layer and the width of the predentin layer did not change with age. Therefore, it is assumed that the equine pulp tissue retained their juvenile status, which explains its unchanged ability to produce high amounts of subocclusal dentin. These preliminary, but clinically significant, findings are worthy of further investigation in order to identify strategies for equine-specific endodontic therapies.
    Language English
    Publishing date 2022-05-30
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2768971-2
    ISSN 2306-7381 ; 2306-7381
    ISSN (online) 2306-7381
    ISSN 2306-7381
    DOI 10.3390/vetsci9060261
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Kategorien

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  5. Article ; Online: Reference gene validation for RT-qPCR, a note on different available software packages.

    De Spiegelaere, Ward / Dern-Wieloch, Jutta / Weigel, Roswitha / Schumacher, Valérie / Schorle, Hubert / Nettersheim, Daniel / Bergmann, Martin / Brehm, Ralph / Kliesch, Sabine / Vandekerckhove, Linos / Fink, Cornelia

    PloS one

    2015  Volume 10, Issue 3, Page(s) e0122515

    Abstract: Background: An appropriate normalization strategy is crucial for data analysis from real time reverse transcription polymerase chain reactions (RT-qPCR). It is widely supported to identify and validate stable reference genes, since no single biological ... ...

    Abstract Background: An appropriate normalization strategy is crucial for data analysis from real time reverse transcription polymerase chain reactions (RT-qPCR). It is widely supported to identify and validate stable reference genes, since no single biological gene is stably expressed between cell types or within cells under different conditions. Different algorithms exist to validate optimal reference genes for normalization. Applying human cells, we here compare the three main methods to the online available RefFinder tool that integrates these algorithms along with R-based software packages which include the NormFinder and GeNorm algorithms.
    Results: 14 candidate reference genes were assessed by RT-qPCR in two sample sets, i.e. a set of samples of human testicular tissue containing carcinoma in situ (CIS), and a set of samples from the human adult Sertoli cell line (FS1) either cultured alone or in co-culture with the seminoma like cell line (TCam-2) or with equine bone marrow derived mesenchymal stem cells (eBM-MSC). Expression stabilities of the reference genes were evaluated using geNorm, NormFinder, and BestKeeper. Similar results were obtained by the three approaches for the most and least stably expressed genes. The R-based packages NormqPCR, SLqPCR and the NormFinder for R script gave identical gene rankings. Interestingly, different outputs were obtained between the original software packages and the RefFinder tool, which is based on raw Cq values for input. When the raw data were reanalysed assuming 100% efficiency for all genes, then the outputs of the original software packages were similar to the RefFinder software, indicating that RefFinder outputs may be biased because PCR efficiencies are not taken into account.
    Conclusions: This report shows that assay efficiency is an important parameter for reference gene validation. New software tools that incorporate these algorithms should be carefully validated prior to use.
    MeSH term(s) Algorithms ; Cell Line ; Coculture Techniques ; Humans ; Reverse Transcriptase Polymerase Chain Reaction/methods ; Reverse Transcriptase Polymerase Chain Reaction/standards ; Software
    Language English
    Publishing date 2015-03-31
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Validation Study
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0122515
    Database MEDical Literature Analysis and Retrieval System OnLINE

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