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  1. Book ; Conference proceedings: XVIth International Conference on Dioxygen Binding and Sensing Proteins (O2BIP)

    Dewilde, Sylvia

    (IUBMB life ; 63.2011,5)

    2011  

    Title variant Sixteenth
    Event/congress International Conference on Dioxygen Binding and Sensing Proteins (16, 2010.08.22-26, Anwerp) ; O2BIP (16, 2010.08.22-26, Anwerp)
    Author's details guest ed.: Sylvia Dewilde
    Series title IUBMB life ; 63.2011,5
    Language English
    Size S. 287 - 371
    Publisher Wiley
    Publishing place Hoboken, NJ
    Document type Book ; Conference proceedings
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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  2. Article ; Online: Structural and dynamic characterization of the hexa-coordinated globin from Spisula solidissima.

    Pesce, Alessandra / Barmpidi, Katerina / Dewilde, Sylvia / Estarellas, Carolina / Moens, Luc / Bolognesi, Martino / Luque, Francisco Javier / Nardini, Marco

    Journal of inorganic biochemistry

    2023  Volume 246, Page(s) 112289

    Abstract: High energy consumption in the nervous system requires a continuous supply of ... ...

    Abstract High energy consumption in the nervous system requires a continuous supply of O
    MeSH term(s) Animals ; Spisula/metabolism ; Ligands ; Hemoglobins/chemistry ; Heme/chemistry ; Myoglobin ; Oxygen/metabolism
    Chemical Substances Ligands ; Hemoglobins ; Heme (42VZT0U6YR) ; Myoglobin ; Oxygen (S88TT14065)
    Language English
    Publishing date 2023-06-12
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 162843-4
    ISSN 1873-3344 ; 0162-0134
    ISSN (online) 1873-3344
    ISSN 0162-0134
    DOI 10.1016/j.jinorgbio.2023.112289
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.B 1730: Show issues Location:
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  3. Article ; Online: Interaction of nitrite with ferric protoglobin from

    Sgammato, Roberta / Van Brempt, Niels / Aerts, Roy / Van Doorslaer, Sabine / Dewilde, Sylvia / Herrebout, Wouter / Johannessen, Christian

    Dalton transactions (Cambridge, England : 2003)

    2023  Volume 52, Issue 10, Page(s) 2976–2987

    Abstract: Protoglobin ... ...

    Abstract Protoglobin from
    MeSH term(s) Heme/chemistry ; Nitrites ; Methanosarcina/chemistry ; Methanosarcina/metabolism ; Ligands ; Globins/chemistry ; Globins/metabolism ; Iron/metabolism ; Nitric Oxide/metabolism ; Electron Spin Resonance Spectroscopy
    Chemical Substances Heme (42VZT0U6YR) ; Nitrites ; Ligands ; Globins (9004-22-2) ; Iron (E1UOL152H7) ; Nitric Oxide (31C4KY9ESH)
    Language English
    Publishing date 2023-03-07
    Publishing country England
    Document type Journal Article
    ZDB-ID 1472887-4
    ISSN 1477-9234 ; 1364-5447 ; 0300-9246 ; 1477-9226
    ISSN (online) 1477-9234 ; 1364-5447
    ISSN 0300-9246 ; 1477-9226
    DOI 10.1039/d2dt03252j
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: A reliable set of reference genes to normalize oxygen-dependent cytoglobin gene expression levels in melanoma.

    De Backer, Joey / Maric, Darko / Bosman, Matthias / Dewilde, Sylvia / Hoogewijs, David

    Scientific reports

    2021  Volume 11, Issue 1, Page(s) 10879

    Abstract: Cytoglobin (CYGB) is a ubiquitously expressed protein with a protective role against oxidative stress, fibrosis and tumor growth, shown to be transcriptionally regulated under hypoxic conditions. Hypoxia-inducible CYGB expression is observed in several ... ...

    Abstract Cytoglobin (CYGB) is a ubiquitously expressed protein with a protective role against oxidative stress, fibrosis and tumor growth, shown to be transcriptionally regulated under hypoxic conditions. Hypoxia-inducible CYGB expression is observed in several cancer cell lines and particularly in various melanoma-derived cell lines. However, reliable detection of hypoxia-inducible mRNA levels by qPCR depends on the critical choice of suitable reference genes for accurate normalization. Limited evidence exists to support selection of the commonly used reference genes in hypoxic models of melanoma. This study aimed to select the optimal reference genes to study CYGB expression levels in melanoma cell lines exposed to hypoxic conditions (0.2% O
    MeSH term(s) Biomarkers, Tumor ; Cell Line, Tumor ; Cytoglobin/genetics ; Cytoglobin/metabolism ; Gene Expression Regulation ; Humans ; Hypoxia/genetics ; Hypoxia/metabolism ; Melanoma/diagnosis ; Melanoma/genetics ; Melanoma/metabolism ; Oxygen/metabolism ; Protein Stability ; RNA, Messenger/genetics
    Chemical Substances Biomarkers, Tumor ; Cytoglobin ; RNA, Messenger ; Oxygen (S88TT14065)
    Language English
    Publishing date 2021-05-25
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-021-90284-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Neuroglobin Expression in the Brain: a Story of Tissue Homeostasis Preservation.

    Van Acker, Zoë P / Luyckx, Evi / Dewilde, Sylvia

    Molecular neurobiology

    2018  Volume 56, Issue 3, Page(s) 2101–2122

    Abstract: After its discovery in 2000, the notion grew that neuroglobin, a neuronal specific heme protein, is involved in cytoprotection. To date, neuroglobin levels have been positively correlated with a beneficial outcome in a plethora of neurotoxic insults, e.g. ...

    Abstract After its discovery in 2000, the notion grew that neuroglobin, a neuronal specific heme protein, is involved in cytoprotection. To date, neuroglobin levels have been positively correlated with a beneficial outcome in a plethora of neurotoxic insults, e.g., ischemic and traumatic brain injuries and Alzheimer's disease. The first part of this review goes further into these changes of neuroglobin expression upon different neuronal insults as well as the underlying regulation. In the second part, we shed light on the mechanisms by which neuroglobin contributes to neuroprotection, being (i) the scavenging and detoxification of reactive oxygen/nitrogen species, (ii) the augmentation of the threshold for apoptosis initiation, (iii) its contribution to an anti-inflammatory milieu, and (iv) tissue regeneration. We also consider different neuroglobin models to address as yet unanswered questions. Based on the recent findings and progress in the field, we invigorate the avenues of neuroglobin in neurological ailments to increase in the coming years.
    MeSH term(s) Animals ; Apoptosis/physiology ; Brain/metabolism ; Neurogenesis/physiology ; Neuroglobin/metabolism ; Neurons/metabolism ; Neuroprotection/physiology
    Chemical Substances Neuroglobin
    Language English
    Publishing date 2018-07-10
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 645020-9
    ISSN 1559-1182 ; 0893-7648
    ISSN (online) 1559-1182
    ISSN 0893-7648
    DOI 10.1007/s12035-018-1212-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 2411: Show issues Location:
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  6. Article ; Online: The Challenging World of Biofilm Physiology.

    Donné, Joke / Dewilde, Sylvia

    Advances in microbial physiology

    2015  Volume 67, Page(s) 235–292

    Abstract: Worldwide, infectious diseases are one of the leading causes of death among children. At least 65% of all infections are caused by the biofilm mode of bacterial growth. Bacteria colonise surfaces and grow as multicellular biofilm communities surrounded ... ...

    Abstract Worldwide, infectious diseases are one of the leading causes of death among children. At least 65% of all infections are caused by the biofilm mode of bacterial growth. Bacteria colonise surfaces and grow as multicellular biofilm communities surrounded by a polymeric matrix as a common survival strategy. These sessile communities endow bacteria with high tolerance to antimicrobial agents and hence cause persistent and chronic bacterial infections, such as dental caries, periodontitis, otitis media, cystic fibrosis and pneumonia. The highly complex nature and the rapid adaptability of the biofilm population impede our understanding of the process of biofilm formation, but an important role for oxygen-binding proteins herein is clear. Much research on this bacterial lifestyle is already performed, from genome/proteome analysis to in vivo antibiotic susceptibility testing, but without significant progress in biofilm treatment or eradication. This review will present the multiple challenges of biofilm research and discuss possibilities to cross these barriers in future experimental studies.
    MeSH term(s) Bacteria/growth & development ; Bacteria/pathogenicity ; Bacterial Infections/microbiology ; Bacterial Physiological Phenomena ; Biofilms/growth & development ; Humans
    Language English
    Publishing date 2015
    Publishing country England
    Document type Journal Article
    ZDB-ID 174-0
    ISSN 2162-5468 ; 0065-2911
    ISSN (online) 2162-5468
    ISSN 0065-2911
    DOI 10.1016/bs.ampbs.2015.09.003
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 366: Show issues Location:
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  7. Article ; Online: Neuroglobin Expression Models as a Tool to Study Its Function.

    Luyckx, Evi / Van Acker, Zoë P / Ponsaerts, Peter / Dewilde, Sylvia

    Oxidative medicine and cellular longevity

    2019  Volume 2019, Page(s) 5728129

    Abstract: Neuroglobin (Ngb) is an evolutionary conserved member of the globin family with a primary expression in neurons of which the exact functions remain elusive. A plethora ... ...

    Abstract Neuroglobin (Ngb) is an evolutionary conserved member of the globin family with a primary expression in neurons of which the exact functions remain elusive. A plethora of
    MeSH term(s) Animals ; Mice, Transgenic ; Neuroglobin/metabolism
    Chemical Substances Neuroglobin
    Language English
    Publishing date 2019-06-19
    Publishing country United States
    Document type Journal Article ; Review
    ISSN 1942-0994
    ISSN (online) 1942-0994
    DOI 10.1155/2019/5728129
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Exploring three different expression systems for recombinant expression of globins: Escherichia coli, Pichia pastoris and Spodoptera frugiperda.

    Bracke, An / Hoogewijs, David / Dewilde, Sylvia

    Analytical biochemistry

    2017  Volume 543, Page(s) 62–70

    Abstract: Globins are among the best investigated proteins in biological and medical sciences and represent a prime tool for the study of the evolution of genes and the structure-function relationship of proteins. Here, we explore the recombinant expression of ... ...

    Abstract Globins are among the best investigated proteins in biological and medical sciences and represent a prime tool for the study of the evolution of genes and the structure-function relationship of proteins. Here, we explore the recombinant expression of globins in three different expression systems: Escherichia coli, Pichia pastoris and the baculovirus infected Spodoptera frugiperda. We expressed two different human globin types in these three expression systems: I) the well-characterized neuroglobin and II) the uncharacterized, circular permutated globin domain of the large chimeric globin androglobin. It is clear from the literature that E.coli is the most used expression system for expression and purification of recombinant globins. However, the major disadvantage of E. coli is the formation of insoluble aggregates. We experienced that, for more complex multi-domain globins, like the chimeric globin androglobin, it is recommended to switch to a higher eukaryotic expression system.
    MeSH term(s) Animals ; Escherichia coli/genetics ; Gene Expression Profiling ; Globins/genetics ; Humans ; Pichia/genetics ; Real-Time Polymerase Chain Reaction ; Recombinant Proteins/genetics ; Spodoptera/genetics
    Chemical Substances Recombinant Proteins ; Globins (9004-22-2)
    Language English
    Publishing date 2017-12-05
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1110-1
    ISSN 1096-0309 ; 0003-2697
    ISSN (online) 1096-0309
    ISSN 0003-2697
    DOI 10.1016/j.ab.2017.11.027
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 389: Show issues Location:
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  9. Article ; Online: The effect of pH and nitrite on the haem pocket of GLB-33, a globin-coupled neuronal transmembrane receptor of Caenorhabditis elegans.

    Van Brempt, Niels / Sgammato, Roberta / Beirinckx, Quinten / Hammerschmid, Dietmar / Sobott, Frank / Dewilde, Sylvia / Moens, Luc / Herrebout, Wouter / Johannessen, Christian / Van Doorslaer, Sabine

    Biochimica et biophysica acta. Proteins and proteomics

    2023  Volume 1871, Issue 4, Page(s) 140913

    Abstract: Out of the 34 globins in Caenorhabditis elegans, GLB-33 is a putative globin-coupled transmembrane receptor with a yet unknown function. The globin domain (GD) contains a particularly hydrophobic haem pocket, that rapidly oxidizes to a low-spin hydroxide- ...

    Abstract Out of the 34 globins in Caenorhabditis elegans, GLB-33 is a putative globin-coupled transmembrane receptor with a yet unknown function. The globin domain (GD) contains a particularly hydrophobic haem pocket, that rapidly oxidizes to a low-spin hydroxide-ligated haem state at physiological pH. Moreover, the GD has one of the fastest nitrite reductase activity ever reported for globins. Here, we use a combination of electronic circular dichroism, resonance Raman and electron paramagnetic resonance (EPR) spectroscopy with mass spectrometry to study the pH dependence of the ferric form of the recombinantly over-expressed GD in the presence and absence of nitrite. The competitive binding of nitrite and hydroxide is examined as well as nitrite-induced haem modifications at acidic pH. Comparison of the spectroscopic results with data from other haem proteins allows to deduce the important effect of Arg at position E10 in stabilization of exogenous ligands. Furthermore, continuous-wave and pulsed EPR indicate that ligation of nitrite occurs in a nitrito mode at pH 5.0 and above. At pH 4.0, an additional formation of a nitro-bound haem form is observed along with fast formation of a nitri-globin.
    MeSH term(s) Animals ; Globins ; Caenorhabditis elegans/metabolism ; Nitrites/metabolism ; Heme/metabolism ; Hydrogen-Ion Concentration
    Chemical Substances Globins (9004-22-2) ; Nitrites ; Heme (42VZT0U6YR)
    Language English
    Publishing date 2023-03-31
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2918798-9
    ISSN 1878-1454 ; 1570-9639
    ISSN (online) 1878-1454
    ISSN 1570-9639
    DOI 10.1016/j.bbapap.2023.140913
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  10. Article ; Online: Non-Methylation-Linked Mechanism of REST-Induced Neuroglobin Expression Impacts Mitochondrial Phenotypes in a Mouse Model of Amyotrophic Lateral Sclerosis.

    Van Acker, Zoë P / Declerck, Ken / Luyckx, Evi / Vanden Berghe, Wim / Dewilde, Sylvia

    Neuroscience

    2019  Volume 412, Page(s) 233–247

    Abstract: Neuroglobin (Ngb) is a REST/NRSF-regulated protein, active in reactive oxygen species detoxification and cytochrome c inhibition, which provides a beneficial outcome in pathologies as Alzheimer's disease and strokes. Considering that oxidative stress and ...

    Abstract Neuroglobin (Ngb) is a REST/NRSF-regulated protein, active in reactive oxygen species detoxification and cytochrome c inhibition, which provides a beneficial outcome in pathologies as Alzheimer's disease and strokes. Considering that oxidative stress and cell death are typical hallmarks of amyotrophic lateral sclerosis (ALS), we sought to explore Ngb's involvement along this disease progression. Ngb transcription was detected to be two-fold down-regulated in late-stage SOD
    MeSH term(s) Amyotrophic Lateral Sclerosis/genetics ; Amyotrophic Lateral Sclerosis/metabolism ; Animals ; Calcium/metabolism ; Disease Models, Animal ; Mice ; Mice, Knockout ; Mitochondria/genetics ; Mitochondria/metabolism ; Neuroglobin/genetics ; Neuroglobin/metabolism ; Oxidative Stress/physiology ; Reactive Oxygen Species/metabolism ; Spinal Cord/metabolism
    Chemical Substances Neuroglobin ; Reactive Oxygen Species ; Calcium (SY7Q814VUP)
    Language English
    Publishing date 2019-05-31
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 196739-3
    ISSN 1873-7544 ; 0306-4522
    ISSN (online) 1873-7544
    ISSN 0306-4522
    DOI 10.1016/j.neuroscience.2019.05.039
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