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  1. Article: Heightened miR6024-NLR interactions facilitate necrotrophic pathogenesis in tomato

    Dey, Sayani / Sarkar, Arijita / Chowdhury, Shreya / Singh, Raghuvir / Mukherjee, Ananya / Ghosh, Zhumur / Kundu, Pallob

    Plant molecular biology. 2022 Aug., v. 109, no. 6

    2022  

    Abstract: KEY MESSAGE: miR6024 acts as a negative regulator of R genes, hence of Tomato plant immunity, and facilitates disease by the necrotrophic pathogen A. solani. Plant resistance genes or Nucleotide-binding leucine-rich repeat (NLR) genes, integral ... ...

    Abstract KEY MESSAGE: miR6024 acts as a negative regulator of R genes, hence of Tomato plant immunity, and facilitates disease by the necrotrophic pathogen A. solani. Plant resistance genes or Nucleotide-binding leucine-rich repeat (NLR) genes, integral components of plant disease stress-signaling are targeted by variable groups of miRNAs. However, the significance of miRNA-mediated regulation of NLRs during a pathogen stress response, specifically for necrotrophic fungus, is poorly understood. A thorough examination of Tomato NLRs and miRNAs could map substantial interactions of which half the annotated NLRs were targets of Solanaceae-specific and conserved miRNAs, at the NB subdomain. The Solanaceae-specific miR6024 and its NLR targets analysed in different phytopathogenic stresses revealed differential and mutually antagonistic regulation. Interestingly, miR6024-targeted cleavage of a target NLR also triggered the generation of secondary phased siRNAs which could potentially amplify the defense signal. RNA-seq analysis of leaf tissues from miR6024 overexpressing Tomato plants evidenced a perturbation in the defense transcriptome with the transgenics showing unwarranted immune response-related genes’ expression with or without infection with necrotrophic Alternaria solani, though no adverse effect could be observed in the growth and development of the transgenic plants. Transgenic plants exhibited constitutive downregulation of the target NLRs, aggravated disease phenotype with an enhanced lesion, greater ROS generation and hypersusceptibility to A. solani infection, thus establishing that miR6024 negatively impacts plant immune response during necrotrophic pathogenesis. Limited knowledge about the outcome of NLR-miRNA interaction during necrotrophic pathogenesis is a hindrance to the deployment of miRNAs in crop improvement programs. With the elucidation of the necrotrophic disease-synergistic role played by miR6024, it becomes a potent candidate for biotechnological manipulation for the rapid development of pathogen-tolerant solanaceous plants.
    Keywords Alternaria solani ; adverse effects ; fungi ; growth and development ; immune response ; leaves ; microRNA ; molecular biology ; pathogenesis ; pathogens ; phenotype ; sequence analysis ; stress response ; tomatoes ; transcriptome
    Language English
    Dates of publication 2022-08
    Size p. 717-739.
    Publishing place Springer Netherlands
    Document type Article
    ZDB-ID 778032-1
    ISSN 1573-5028 ; 0167-4412
    ISSN (online) 1573-5028
    ISSN 0167-4412
    DOI 10.1007/s11103-022-01270-z
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Einzelsign.: Show issues
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  2. Article ; Online: Heightened miR6024-NLR interactions facilitate necrotrophic pathogenesis in tomato.

    Dey, Sayani / Sarkar, Arijita / Chowdhury, Shreya / Singh, Raghuvir / Mukherjee, Ananya / Ghosh, Zhumur / Kundu, Pallob

    Plant molecular biology

    2022  Volume 109, Issue 6, Page(s) 717–739

    Abstract: Key message: miR6024 acts as a negative regulator of R genes, hence of Tomato plant immunity, and facilitates disease by the necrotrophic pathogen A. solani. Plant resistance genes or Nucleotide-binding leucine-rich repeat (NLR) genes, integral ... ...

    Abstract Key message: miR6024 acts as a negative regulator of R genes, hence of Tomato plant immunity, and facilitates disease by the necrotrophic pathogen A. solani. Plant resistance genes or Nucleotide-binding leucine-rich repeat (NLR) genes, integral components of plant disease stress-signaling are targeted by variable groups of miRNAs. However, the significance of miRNA-mediated regulation of NLRs during a pathogen stress response, specifically for necrotrophic fungus, is poorly understood. A thorough examination of Tomato NLRs and miRNAs could map substantial interactions of which half the annotated NLRs were targets of Solanaceae-specific and conserved miRNAs, at the NB subdomain. The Solanaceae-specific miR6024 and its NLR targets analysed in different phytopathogenic stresses revealed differential and mutually antagonistic regulation. Interestingly, miR6024-targeted cleavage of a target NLR also triggered the generation of secondary phased siRNAs which could potentially amplify the defense signal. RNA-seq analysis of leaf tissues from miR6024 overexpressing Tomato plants evidenced a perturbation in the defense transcriptome with the transgenics showing unwarranted immune response-related genes' expression with or without infection with necrotrophic Alternaria solani, though no adverse effect could be observed in the growth and development of the transgenic plants. Transgenic plants exhibited constitutive downregulation of the target NLRs, aggravated disease phenotype with an enhanced lesion, greater ROS generation and hypersusceptibility to A. solani infection, thus establishing that miR6024 negatively impacts plant immune response during necrotrophic pathogenesis. Limited knowledge about the outcome of NLR-miRNA interaction during necrotrophic pathogenesis is a hindrance to the deployment of miRNAs in crop improvement programs. With the elucidation of the necrotrophic disease-synergistic role played by miR6024, it becomes a potent candidate for biotechnological manipulation for the rapid development of pathogen-tolerant solanaceous plants.
    MeSH term(s) Lycopersicon esculentum/microbiology ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Plant Diseases/genetics ; Plant Diseases/microbiology ; Plant Immunity ; Plants, Genetically Modified/genetics
    Chemical Substances MicroRNAs
    Language English
    Publishing date 2022-05-02
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 778032-1
    ISSN 1573-5028 ; 0167-4412
    ISSN (online) 1573-5028
    ISSN 0167-4412
    DOI 10.1007/s11103-022-01270-z
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3458: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article: Analysis of Water Quantity and Quality Trade-Offs to Inform Selective Harvesting of Inflows in Complex Water Resource Systems

    Dey, Sayani / Barton, Andrew / Kandra, Harpreet / Bagirov, Adil / Wilson, Kym

    Water resources management. 2021 Sept., v. 35, no. 12

    2021  

    Abstract: Challenges faced by water resource systems are multi-faceted. The problem can be even more pronounced in a dry continent like Australia where the water resources can often be afflicted by high salinity and turbidity. Therefore, modern water resource ... ...

    Abstract Challenges faced by water resource systems are multi-faceted. The problem can be even more pronounced in a dry continent like Australia where the water resources can often be afflicted by high salinity and turbidity. Therefore, modern water resource systems require to appropriately manage both water quality and quantity. This study aims to illustrate the trade-offs between water quantity and quality in a reservoir, based on decisions to harvest different inflow sources. Taylors Lake of the Grampians reservoir system in Western Victoria, Australia was chosen as the case study for this research as it is sufficiently complex and includes many of the contemporary water resources challenges seen around the world. Different operational scenarios were analysed which included increasingly stringent water quality criteria before the water was harvested or otherwise allowed to by-pass the storage. The study suggests that selective harvesting of water can be an option to improve the overall and long-term water quality within a reservoir, but stringent water quality measures can lead to an associated loss of overall water quantity. This research study provides useful insight to water planners and stakeholders in similar catchment settings around the world, to identify water harvesting regimes with competing water quality constraints.
    Keywords administrative management ; case studies ; lakes ; salinity ; stakeholders ; turbidity ; water ; water analysis ; water quality ; water quantity ; watersheds
    Language English
    Dates of publication 2021-09
    Size p. 4149-4165.
    Publishing place Springer Netherlands
    Document type Article
    ZDB-ID 59924-4
    ISSN 1573-1650 ; 0920-4741
    ISSN (online) 1573-1650
    ISSN 0920-4741
    DOI 10.1007/s11269-021-02936-x
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 6155: Show issues

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  4. Article ; Online: Integrated miRNA and mRNA expression profiling reveals the response regulators of a susceptible tomato cultivar to early blight disease.

    Sarkar, Deepti / Maji, Ranjan Kumar / Dey, Sayani / Sarkar, Arijita / Ghosh, Zhumur / Kundu, Pallob

    DNA research : an international journal for rapid publication of reports on genes and genomes

    2017  Volume 24, Issue 3, Page(s) 235–250

    Abstract: Early blight, caused by the fungus Alternaria solani, is a devastating foliar disease of tomatoes, causes massive yield loss each year worldwide. Molecular basis of the compatible host-pathogen interaction was elusive. We adopted next generation ... ...

    Abstract Early blight, caused by the fungus Alternaria solani, is a devastating foliar disease of tomatoes, causes massive yield loss each year worldwide. Molecular basis of the compatible host-pathogen interaction was elusive. We adopted next generation sequencing approach to decipher miRNAs and mRNAs that are differentially expressed during Alternaria-stress in tomato. Some of the interesting findings were also validated by alternative techniques. Our analysis revealed 181 known-miRNAs, belonging to 121 miRNA families, of which 67 miRNAs showed at least 2-fold change in expression level with the majority being downregulated. Concomitantly, 5,450 mRNAs were significantly regulated in the same diseased tissues. Differentially expressed genes were most significantly associated with response to stimulus process, photosynthesis, biosynthesis of secondary metabolites, plant-pathogen interaction and plant hormone signal transduction pathways. GO term enrichment-based categorization of gene-functions further supported this observation, as terms related to pathogen perception, disease signal transduction, cellular metabolic processes including oxidoreductase and kinase activity were over represented. In addition, we have discovered 102 miRNA-mRNA pairs which were regulated antagonistically, and careful study of the targeted mRNAs depicted that multiple transcription factors, nucleotide-binding site leucine-rich repeats, receptor-like proteins and enzymes related to cellular ROS management were profoundly affected. These studies have identified key regulators of Alternaria-stress response in tomato and the subset of genes that are likely to be post-transcriptionally silenced during the infection.
    MeSH term(s) Alternariosis/genetics ; Disease Susceptibility ; Gene Expression Regulation, Plant ; Genes, Plant ; High-Throughput Nucleotide Sequencing ; Lycopersicon esculentum/genetics ; Lycopersicon esculentum/physiology ; MicroRNAs ; Plant Diseases/genetics ; RNA, Messenger ; RNA, Plant ; Transcriptome
    Chemical Substances MicroRNAs ; RNA, Messenger ; RNA, Plant
    Language English
    Publishing date 2017-06-01
    Publishing country England
    Document type Journal Article
    ZDB-ID 1212508-8
    ISSN 1756-1663 ; 1340-2838
    ISSN (online) 1756-1663
    ISSN 1340-2838
    DOI 10.1093/dnares/dsx003
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4123: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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