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  1. Article ; Online: Small noncoding RNAs and sperm nuclear basic proteins reflect the environmental impact on germ cells.

    Ferrero, Giulio / Festa, Rosaria / Follia, Laura / Lettieri, Gennaro / Tarallo, Sonia / Notari, Tiziana / Giarra, Antonella / Marinaro, Carmela / Pardini, Barbara / Marano, Alessandra / Piaggeschi, Giulia / Di Battista, Carla / Trifuoggi, Marco / Piscopo, Marina / Montano, Luigi / Naccarati, Alessio

    Molecular medicine (Cambridge, Mass.)

    2024  Volume 30, Issue 1, Page(s) 12

    Abstract: Background: Molecular techniques can complement conventional spermiogram analyses to provide new information on the fertilizing potential of spermatozoa and to identify early alterations due to environmental pollution.: Methods: Here, we present a ... ...

    Abstract Background: Molecular techniques can complement conventional spermiogram analyses to provide new information on the fertilizing potential of spermatozoa and to identify early alterations due to environmental pollution.
    Methods: Here, we present a multilevel molecular profiling by small RNA sequencing and sperm nuclear basic protein analysis of male germ cells from 33 healthy young subjects residing in low and high-polluted areas.
    Results: Although sperm motility and sperm concentration were comparable between samples from the two sites, those from the high-pollution area had a higher concentration of immature/immune cells, a lower protamine/histone ratio, a reduced ability of sperm nuclear basic proteins to protect DNA from oxidative damage, and an altered copper/zinc ratio in sperm. Sperm levels of 32 microRNAs involved in intraflagellar transport, oxidative stress response, and spermatogenesis were different between the two areas. In parallel, a decrease of Piwi-interacting RNA levels was observed in samples from the high-polluted area.
    Conclusions: This comprehensive analysis provides new insights into pollution-driven epigenetic alterations in sperm not detectable by spermiogram.
    MeSH term(s) Male ; Humans ; RNA, Small Untranslated/genetics ; RNA, Small Untranslated/metabolism ; Semen ; Sperm Motility ; Spermatozoa/metabolism ; Environment ; Nuclear Proteins
    Chemical Substances sperm basic nuclear proteins ; RNA, Small Untranslated ; Nuclear Proteins
    Language English
    Publishing date 2024-01-20
    Publishing country England
    Document type Journal Article
    ZDB-ID 1283676-x
    ISSN 1528-3658 ; 1076-1551
    ISSN (online) 1528-3658
    ISSN 1076-1551
    DOI 10.1186/s10020-023-00776-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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    Jg. 1995 - 2021: Lesesall (2.OG)
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  2. Article ; Online: The 8q24 region hosts miRNAs altered in biospecimens of colorectal and bladder cancer patients.

    Gagliardi, Amedeo / Francescato, Giulia / Ferrero, Giulio / Birolo, Giovanni / Tarallo, Sonia / Francavilla, Antonio / Piaggeschi, Giulia / Di Battista, Carla / Gallo, Gaetano / Realis Luc, Alberto / Sacerdote, Carlotta / Matullo, Giuseppe / Vineis, Paolo / Naccarati, Alessio / Pardini, Barbara

    Cancer medicine

    2022  Volume 12, Issue 5, Page(s) 5859–5873

    Abstract: Background: The 8q24 locus is enriched in cancer-associated polymorphisms and, despite containing relatively few protein-coding genes, it hosts the MYC oncogene and other genetic elements connected to tumorigenesis, including microRNAs (miRNAs). ... ...

    Abstract Background: The 8q24 locus is enriched in cancer-associated polymorphisms and, despite containing relatively few protein-coding genes, it hosts the MYC oncogene and other genetic elements connected to tumorigenesis, including microRNAs (miRNAs). Research on miRNAs may provide insights into the transcriptomic regulation of this multiple cancer-associated region.
    Material and methods: We profiled all miRNAs located in the 8q24 region in 120 colorectal cancer (CRC) patients and 80 controls. miRNA profiling was performed on cancer/non-malignant adjacent mucosa, stool, and plasma extracellular vesicles (EVs), and the results validated with The Cancer Genome Atlas (TCGA) data. To verify if the 8q24-annotated miRNAs altered in CRC were dysregulated in other cancers and biofluids, we evaluated their levels in bladder cancer (BC) cases from the TCGA dataset and in urine and plasma EVs from a set of BC cases and healthy controls.
    Results: Among the detected mature miRNAs in the region, 12 were altered between CRC and adjacent mucosa (adj. p < 0.05). Five and four miRNAs were confirmed as dysregulated in the CRC and BC TCGA dataset, respectively. A co-expression analysis of tumor/adjacent tissue data from the CRC group revealed a correlation between the dysregulated miRNAs and CRC-related genes (PVT1 and MYC) annotated in 8q24 region. miR-30d-5p and miR-151a-3p, altered in CRC tissue, were also dysregulated in stool of CRC patients and urine of BC cases, respectively. Functional enrichment of dysregulated miRNA target genes highlighted terms related to TP53-mediated cell cycle control.
    Conclusions: Altered expression of 8q24-annotated miRNAs may be relevant for the initiation and/or progression of cancer.
    MeSH term(s) Humans ; MicroRNAs/metabolism ; Gene Expression Profiling/methods ; Transcriptome ; Urinary Bladder Neoplasms/genetics ; Colorectal Neoplasms/pathology ; Gene Expression Regulation, Neoplastic ; Biomarkers, Tumor/metabolism
    Chemical Substances MicroRNAs ; Biomarkers, Tumor
    Language English
    Publishing date 2022-11-10
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2659751-2
    ISSN 2045-7634 ; 2045-7634
    ISSN (online) 2045-7634
    ISSN 2045-7634
    DOI 10.1002/cam4.5375
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Process analysis and global optimization for the microencapsulation of phytosterols by spray drying

    Di Battista, Carla Agustina / Diana Constenla / Juliana Piña / María Verónica Ramírez Rigo

    Powder technology. 2017 Nov., v. 321

    2017  

    Abstract: The response surface methodology (RSM) was used to optimize the microencapsulation of phytosterols by spray drying. The independent variables were drying air inlet temperature, atomization air flowrate, feed flowrate, phytosterols and total solids ... ...

    Abstract The response surface methodology (RSM) was used to optimize the microencapsulation of phytosterols by spray drying. The independent variables were drying air inlet temperature, atomization air flowrate, feed flowrate, phytosterols and total solids contents and the mass ratio between wall materials (Arabic gum and maltodextrin). The analyzed responses were process yield, mean volume particle size of product microparticles, phytosterols retention and encapsulation efficiency. Statistical analysis revealed that the selected independent variables, especially the atomization air flowrate and feed phytosterols content, significantly affect the studied responses. Taking into account the observed results and the analysis of variance, all the responses were successfully adjusted to second order models with interactions, showing good R2 values and correlating the experimental data properly. The product microparticles were also obtained by using the predicted optimal operating and formulation variables to test the validity of the quadratic models. The experimental responses were found to be in agreement with the predicted values and were within the acceptable limits, indicating the suitability of the model for predicting key parameters related to process performance and product quality. The recommended optimal formulation and operating conditions for microencapsulation of phytosterols by spray drying are: drying air temperature of 160°C, atomization air and feed flowrates of 498L/h and 2.5mL/min (equivalent to 42mm of height of rotameter and 7% pump scale, respectively), phytosterols and total solids concentrations of 2 and 15g/100mL, respectively, and mass ratio between Arabic gum and maltodextrin of 2.06. The process yield, encapsulation efficiency and phytosterols retention obtained under the optimum conditions were 84, 72 and 76%, respectively. The product microparticles had a mean volume particle size of about 5μm, well below the more restricted upper size limit of 25μm required to guarantee the incorporation of PS into the intestine micellar phase.
    Keywords air ; analysis of variance ; atomization ; gum arabic ; maltodextrins ; microencapsulation ; microparticles ; models ; particle size ; phytosterols ; powders ; prediction ; product quality ; response surface methodology ; spray drying ; temperature ; total solids
    Language English
    Dates of publication 2017-11
    Size p. 55-65.
    Publishing place Elsevier B.V.
    Document type Article
    ISSN 0032-5910
    DOI 10.1016/j.powtec.2017.08.008
    Database NAL-Catalogue (AGRICOLA)

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  4. Article: The use of arabic gum, maltodextrin and surfactants in the microencapsulation of phytosterols by spray drying

    Di Battista, Carla Agustina / Diana Constenla / Juliana Piña / María Verónica Ramírez-Rigo

    Powder technology. 2015 Dec., v. 286

    2015  

    Abstract: The addition of phytosterols in aqueous-based food matrices is challenging because of their poor physicochemical properties (non-water soluble and hydrophobic powder). By using spray drying, phytosterols microparticles were formulated and developed in ... ...

    Abstract The addition of phytosterols in aqueous-based food matrices is challenging because of their poor physicochemical properties (non-water soluble and hydrophobic powder). By using spray drying, phytosterols microparticles were formulated and developed in this work. Arabic gum, maltodextrin and one of two different surfactants were thoroughly studied as wall materials. Increasing concentrations of Tween 20 (T20) or sodium lauryl sulfate (SDS), from 0.1 to 2.65% w/v, were evaluated. The feed suspension characteristics (viscosity, interfacial properties and particle size distribution), process yield (PY), encapsulation efficiency (EE), phytosterols retention (R) and size of the microparticles were analyzed. The presence of surfactants in the suspension to be spray dried has significant effects on the studied responses. T20 led to process yields around 65% (2% w/v surfactant concentration). On the other hand, the microparticles obtained using 2% w/v of SDS were the best in terms of EE (about 50%), R (close to 40%) and particle size (5.89μm), being the PY acceptable (almost 55%). According to the open literature, which indicates that average particle sizes lower than 25μm favor the phytosterols bioavailability, the microparticles obtained in this work are promising for phytosterols delivery.
    Keywords bioavailability ; food matrix ; gum arabic ; hydrophobicity ; maltodextrins ; microencapsulation ; microparticles ; particle size ; particle size distribution ; phytosterols ; polysorbates ; powders ; sodium dodecyl sulfate ; spray drying ; surfactants ; viscosity
    Language English
    Dates of publication 2015-12
    Size p. 193-201.
    Publishing place Elsevier B.V.
    Document type Article
    ISSN 0032-5910
    DOI 10.1016/j.powtec.2015.08.016
    Database NAL-Catalogue (AGRICOLA)

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