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  1. Article: Challenges in Direct Detection of Flaviviruses: A Review.

    Dias, Bruna de Paula / Barbosa, Camila Cavadas / Ferreira, Cyntia Silva / Mayra Soares Alves Dos Santos, Samara / Arrieta, Orlando Alfredo Pineda / Malta, Wellington Carvalho / Gomes, Maria Laura Maximiano Dias / Alves E Silva, Mariela / Fonseca, Júlia de Matos / Borges, Lysandro Pinto / Silva, Breno de Mello

    Pathogens (Basel, Switzerland)

    2023  Volume 12, Issue 5

    Abstract: Arthropods transmit arboviruses via mosquito and tick bites to humans and other animals. The genus flavivirus, which causes diseases, sequelae, and thousands of deaths, mainly in developing and underdeveloped countries, is among the arboviruses of ... ...

    Abstract Arthropods transmit arboviruses via mosquito and tick bites to humans and other animals. The genus flavivirus, which causes diseases, sequelae, and thousands of deaths, mainly in developing and underdeveloped countries, is among the arboviruses of interest to public health. Given the importance of early and accurate diagnosis, this review analyzes the methods of direct detection of flaviviruses, such as reverse transcription loop-mediated isothermal amplification, microfluidics, localized surface plasmon resonance, and surface-enhanced Raman scattering, and presents the advantages, disadvantages, and detection limits identified in studies reported in the literature for each methodology. Among the different methods available, it is essential to balance four fundamental indicators to determine the ideal test: good sensitivity, high specificity, low false positive rate, and rapid results. Among the methods analyzed, reverse transcription loop-mediated isothermal amplification stands out, owing to result availability within a few minutes, with good sensitivity and specificity; in addition, it is the best-characterized methodology.
    Language English
    Publishing date 2023-04-26
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2695572-6
    ISSN 2076-0817
    ISSN 2076-0817
    DOI 10.3390/pathogens12050643
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: SARS-CoV-2 mutations and where to find them: an

    Gonçalves, Ricardo Lemes / Leite, Túlio César Rodrigues / Dias, Bruna de Paula / Caetano, Camila Carla da Silva / de Souza, Ana Clara Gomes / Batista, Ubiratan da Silva / Barbosa, Camila Cavadas / Reyes-Sandoval, Arturo / Coelho, Luiz Felipe Leomil / Silva, Breno de Mello

    Journal of biomolecular structure & dynamics

    2020  Volume 40, Issue 7, Page(s) 3336–3346

    Abstract: Communicated by Ramaswamy H. Sarma. ...

    Abstract Communicated by Ramaswamy H. Sarma.
    MeSH term(s) COVID-19 ; Humans ; Molecular Docking Simulation ; Molecular Dynamics Simulation ; Mutation ; SARS-CoV-2/genetics ; Spike Glycoprotein, Coronavirus/chemistry ; Spike Glycoprotein, Coronavirus/genetics ; Spike Glycoprotein, Coronavirus/metabolism
    Chemical Substances Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2
    Keywords covid19
    Language English
    Publishing date 2020-11-06
    Publishing country England
    Document type Letter ; Research Support, Non-U.S. Gov't
    ZDB-ID 49157-3
    ISSN 1538-0254 ; 0739-1102
    ISSN (online) 1538-0254
    ISSN 0739-1102
    DOI 10.1080/07391102.2020.1844052
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2093: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: Rapid diagnosis of COVID-19 in the first year of the pandemic: A systematic review.

    Borges, Lysandro Pinto / Martins, Aline Fagundes / Silva, Breno de Mello / Dias, Bruna de Paula / Gonçalves, Ricardo Lemes / Souza, Daniela Raguer Valadão de / Oliveira, Makson Gleydson Brito de / Jesus, Pamela Chaves de / Serafini, Mairim Russo / Quintans, Jullyana Souza Siqueira / Coutinho, Henrique Douglas Melo / Martins, Natália / Júnior, Lucindo José Quintans

    International immunopharmacology

    2021  Volume 101, Issue Pt A, Page(s) 108144

    Abstract: Background: COVID-19 caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a global health threat and remains a challenge for modern medicine. Rapid and accurate diagnosis of COVID-19 is vital for proper disease and outbreak ... ...

    Abstract Background: COVID-19 caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a global health threat and remains a challenge for modern medicine. Rapid and accurate diagnosis of COVID-19 is vital for proper disease and outbreak management. Our review aimed to analyze scientific articles published in the literature addressing the rapid tests available for COVID-19 diagnosis at the first year of the pandemic.
    Methods: A systematic review was performed from October 22 to 27, 2020, searching data published in PubMed and Google Scholar databases, using subject headings or keywords related to point of care and rapid test diagnostic for SARS-CoV-2 and COVID-19.
    Results: The first survey identified 403 articles, but only 23 met the defined criteria for the systematic analysis. The sensitivity and specificity parameters were assessed in 19 studies, and the data suggested that there was lower sensitivity in the period 1 to 7 days after the emergence of symptoms (∼38%) higher sensitivity at 8 to 14 days (∼90%), and the highest at 15 to 39 days (∼98%). Accuracy was reported in six studies, reporting values above 50%. Only three studies reported a possible cross-reaction.
    Conclusions: Our findings indicate that the rapid tests used in the first year of the pandemic were tested with a small number of samples and not adequately validated. And the studies that described them were conducted with little scientific rigor.
    MeSH term(s) Antigens, Viral/analysis ; COVID-19/diagnosis ; COVID-19 Testing/standards ; Cross Reactions ; Humans ; Pandemics ; Point-of-Care Testing/standards ; Sensitivity and Specificity
    Chemical Substances Antigens, Viral
    Language English
    Publishing date 2021-09-15
    Publishing country Netherlands
    Document type Journal Article ; Systematic Review
    ZDB-ID 2043785-7
    ISSN 1878-1705 ; 1567-5769
    ISSN (online) 1878-1705
    ISSN 1567-5769
    DOI 10.1016/j.intimp.2021.108144
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5408: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: SARS-CoV-2 mutations and where to find them: An in silico perspective of structural changes and antigenicity of the Spike protein

    Goncalves, Ricardo Lemes / Leite, Tulio Cesar Rodrigues / Dias, Bruna de Paula / Caetano, Camila Carla da Silva / Souza, Ana Clara Gomes de / Batista, Ubiratan da Silva / Barbosa, Camila Cavadas / Reyes-Sandoval, Arturo / Coelho, Luiz Felipe Leomil / Silva, Breno de Mello

    bioRxiv

    Abstract: The recent emergence of a novel coronavirus (SARS-CoV-2) is causing a severe global health threat characterized by severe acute respiratory syndrome (Covid-19). At the moment, there is no specific treatment for this disease, and vaccines are still under ... ...

    Abstract The recent emergence of a novel coronavirus (SARS-CoV-2) is causing a severe global health threat characterized by severe acute respiratory syndrome (Covid-19). At the moment, there is no specific treatment for this disease, and vaccines are still under development. The structural protein Spike is essential for virus infection and has been used as the main target for vaccine and serological diagnosis test development. We analysed 2363 sequences of the Spike protein from SARS-CoV-2 isolates and identified variability in 44 amino acid residues and their worldwide distribution in all continents. We used the three-dimensional structure of the homo-trimer model to predict conformational epitopes of B-cell, and sequence of Spike protein Wuhan-Hu-1 to predict linear epitopes of T-Cytotoxic and T-Helper cells. We identified 45 epitopes with amino acid variations. Finally, we showed the distribution of mutations within the epitopes. Our findings can help researches to identify more efficient strategies for the development of vaccines, therapies, and serological diagnostic tests based on the Spike protein of Sars-Cov-2.
    Keywords covid19
    Publisher BioRxiv; WHO
    Document type Article ; Online
    Note WHO #Covidence: #108563
    DOI 10.1101/2020.05.21.108563
    Database COVID19

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  5. Article ; Online: SARS-CoV-2 mutations and where to find them

    Gonçalves, Ricardo Lemes / Leite, Túlio César Rodrigues / Dias, Bruna de Paula / Caetano, Camila Carla da Silva / de Souza, Ana Clara Gomes / Batista, Ubiratan da Silva / Barbosa, Camila Cavadas / Reyes-Sandoval, Arturo / Coelho, Luiz Felipe Leomil / Silva, Breno de Mello

    Journal of Biomolecular Structure and Dynamics

    an in silico perspective of structural changes and antigenicity of the spike protein

    2020  , Page(s) 1–11

    Keywords Molecular Biology ; Structural Biology ; General Medicine ; covid19
    Language English
    Publisher Informa UK Limited
    Publishing country uk
    Document type Article ; Online
    ZDB-ID 49157-3
    ISSN 1538-0254 ; 0739-1102
    ISSN (online) 1538-0254
    ISSN 0739-1102
    DOI 10.1080/07391102.2020.1844052
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2093: Show issues Location:
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