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  1. Article ; Online: Abrus precatorius Leaf Extract Stimulates Insulin-mediated Muscle Glucose Uptake: In vitro Studies and Phytochemical Analysis.

    Lankatillake, Chintha / Huynh, Tien / Dias, Daniel A

    Planta medica

    2024  Volume 90, Issue 5, Page(s) 388–396

    Abstract: Diabetes mellitus, linked with insulin resistance and hyperglycaemia, is a leading cause of mortality. Glucose uptake through glucose transporter type 4, especially in skeletal muscle, is crucial for maintaining euglycaemia and is a key pathway targeted ... ...

    Abstract Diabetes mellitus, linked with insulin resistance and hyperglycaemia, is a leading cause of mortality. Glucose uptake through glucose transporter type 4, especially in skeletal muscle, is crucial for maintaining euglycaemia and is a key pathway targeted by antidiabetic medication.
    MeSH term(s) Animals ; Insulin/metabolism ; Proto-Oncogene Proteins c-akt/metabolism ; Abrus/chemistry ; Insulin Receptor Substrate Proteins/metabolism ; Rosiglitazone/metabolism ; Rosiglitazone/pharmacology ; Glucose Transporter Type 4 ; Phosphatidylinositol 3-Kinases ; Muscle, Skeletal/metabolism ; Diabetes Mellitus/drug therapy ; Hypoglycemic Agents/pharmacology ; Insulin Resistance ; Plant Extracts/chemistry ; Hyperglycemia ; Glucose/pharmacology
    Chemical Substances Insulin ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; Insulin Receptor Substrate Proteins ; Rosiglitazone (05V02F2KDG) ; Glucose Transporter Type 4 ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Hypoglycemic Agents ; Plant Extracts ; Glucose (IY9XDZ35W2)
    Language English
    Publishing date 2024-03-15
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 123545-x
    ISSN 1439-0221 ; 0032-0943
    ISSN (online) 1439-0221
    ISSN 0032-0943
    DOI 10.1055/a-2281-0988
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  2. Article: Abrus precatorius Leaf Extract Stimulates Insulin-mediated Muscle Glucose Uptake: In vitro Studies and Phytochemical Analysis

    Lankatillake, Chintha / Huynh, Tien / Dias, Daniel A

    Planta Medica

    2024  Volume 90, Issue 05, Page(s) 388–396

    Abstract: Diabetes mellitus, linked with insulin resistance and hyperglycaemia, is a leading cause of mortality. Glucose uptake through glucose transporter type 4, especially in skeletal muscle, is crucial for maintaining euglycaemia and is a key pathway targeted ... ...

    Abstract Diabetes mellitus, linked with insulin resistance and hyperglycaemia, is a leading cause of mortality. Glucose uptake through glucose transporter type 4, especially in skeletal muscle, is crucial for maintaining euglycaemia and is a key pathway targeted by antidiabetic medication. Abrus precatorius is a medicinal plant with demonstrated antihyperglycaemic activity in animal models, but its mechanisms are unclear. This study evaluated the effect of a 50% ethanolic ( v/v A. precatorius leaf extract on (1) insulin-stimulated glucose uptake and (2) related gene expression in differentiated C2C12 myotubes using rosiglitazone as a positive control, and (3) generated a comprehensive phytochemical profile of A. precatorius leaf extract using liquid chromatography-high resolution mass spectrometry to elucidate its antidiabetic compounds. A. precatorius leaf extract significantly increased insulin-stimulated glucose uptake, and insulin receptor substrate 1 and Akt substrate of 160 kDa gene expression; however, it had no effect on glucose transporter type 4 gene expression. At 250 µg/mL A. precatorius leaf extract, the increase in glucose uptake was significantly higher than 1 µM rosiglitazone. Fifty-five phytochemicals (primarily polyphenols, triterpenoids, saponins, and alkaloids) were putatively identified, including 24 that have not previously been reported from A. precatorius leaves. Abrusin, precatorin I, glycyrrhizin, hemiphloin, isohemiphloin, hispidulin 4′- O β -D-glucopyranoside, homoplantaginin, and cirsimaritin were putatively identified as known major compounds previously reported from A. precatorius leaf extract. A. precatorius leaves contain antidiabetic phytochemicals and enhance insulin-stimulated glucose uptake in myotubes via the protein kinase B/phosphoinositide 3-kinase pathway by regulating insulin receptor substrate 1 and Akt substrate of 160 kDa gene expression. Therefore, A. precatorius leaves may improve skeletal muscle insulin sensitivity and hyperglycaemia. Additionally, it is a valuable source of bioactive phytochemicals with potential therapeutic use for diabetes.
    Keywords Fabaceae ; diabetes mellitus ; LC-HRMS ; hyperglycaemia ; GLUT4
    Language English
    Publishing date 2024-03-15
    Publisher Georg Thieme Verlag KG
    Publishing place Stuttgart ; New York
    Document type Article
    ZDB-ID 123545-x
    ISSN 1439-0221 ; 0032-0943
    ISSN (online) 1439-0221
    ISSN 0032-0943
    DOI 10.1055/a-2281-0988
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  3. Article ; Online: Phenolic compounds-containing fruit peel extracts of Garcinia humilis exhibit anti-melanoma activity

    Poojary, Mahesha M. / Nguyen, Thi Dao / Dekiwadia, Chaitali / Dias, Daniel A. / Huynh, Tien

    Food Bioscience. 2023 Apr., v. 52 p.102428-

    2023  

    Abstract: There has been considerable interest in commercializing fruits of Garcinia humilis due to its pleasant taste and nutritional quality. However, the industrial processing of the fruit generates the peel as waste and this study focussed on upcycling of the ... ...

    Abstract There has been considerable interest in commercializing fruits of Garcinia humilis due to its pleasant taste and nutritional quality. However, the industrial processing of the fruit generates the peel as waste and this study focussed on upcycling of the peel for its anti-melanoma activity at different extraction conditions (24 °C and 37 °C for 24 h, and 70 °C for 1 h). Although the extraction yield was highest at 70 °C, the phenolics and flavonoids extracted, and anti-cancer activity was highest when extracted at 37 °C. Polyphenolic compounds including epicatechin, procyanidins, mangostanol, and gamma-mangostin were tentatively identified based on LC-Orbitrap MS/MS analysis. These extracts showed dose-dependent anti-melanoma activity on MM418C1 (BRAF mutated oncogene) and D24 (wild type BRAF oncogene) cells with IC₅₀ in the range of 130–200 μg/mL and 122–176 μg/mL. Furthermore, the extracts had little effect on control fibroblast cells, indicating selective activity on cancer cells, and displayed morphological changes indicative of apoptosis. Although these extracts were 10 fold less effective compared to anti-cancer drug controls, cisplatin and vemurafenib, the moderate anti-cancer activity of Garcinia peel provides future opportunities for upcycling of this discarded waste as a functional food ingredient.
    Keywords Garcinia humilis ; antineoplastic activity ; apoptosis ; cisplatin ; dose response ; epicatechin ; fibroblasts ; food composition ; fruit peels ; fruits ; functional foods ; nutritive value ; oncogenes ; procyanidins ; taste ; wastes ; Achacha ; Phenolics ; Anti-melanoma ; Anticancer
    Language English
    Dates of publication 2023-04
    Publishing place Elsevier Ltd
    Document type Article ; Online
    Note Use and reproduction
    ISSN 2212-4292
    DOI 10.1016/j.fbio.2023.102428
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: Utilization of GC-MS untargeted metabolomics to assess the delayed response of glufosinate treatment of transgenic herbicide resistant (HR) buffalo grasses (Stenotaphrum secundatum L.).

    Boonchaisri, Siriwat / Stevenson, Trevor / Dias, Daniel A

    Metabolomics : Official journal of the Metabolomic Society

    2020  Volume 16, Issue 2, Page(s) 22

    Abstract: Introduction: Herbicide resistant (HR) buffalo grasses were genetically engineered to resist the non-selective herbicide, glufosinate in order to facilitate a modern, 'weeding program' which is highly effective in terms of minimizing costs and labor. ... ...

    Abstract Introduction: Herbicide resistant (HR) buffalo grasses were genetically engineered to resist the non-selective herbicide, glufosinate in order to facilitate a modern, 'weeding program' which is highly effective in terms of minimizing costs and labor. The resistant trait was conferred by an insertion of the pat gene to allow for the production of the enzyme phosphinothricin acetyltransferase (PAT) to detoxify the glufosinate inhibitive effect. To date, there are only a few reports using metabolomics as well as molecular characterizations published for glufosinate-resistant crops with no reports on HR turfgrass. Therefore, for the first time, this study examines the metabolome of glufosinate-resistant buffalo grasses which not only will be useful to future growers but also the scientific community.
    Objective: A major aim of this present work is to characterize and evaluate the metabolic alterations which may arise from a genetic transformation of HR buffalo grasses by comprehensively using gas chromatography-mass spectrometry (GC-MS) based untargeted metabolomics.
    Methods: Eight-week old plants of 4 HR buffalo grasses, (93-1A, 93-2B, 93-3C and 93-5A) and 3 wild type varieties (WT 8-4A, WT 9-1B and WT 9-1B) were selected for physiological, molecular and metabolomics experiments. Plants were either sprayed with 1, 5, 10 and 15% v/v of glufosinate to evaluate the visual injuries or submerged in 5% v/v of glufosinate 3 days prior to a GC-MS based untargeted metabolomics analysis. In contrast, the control group was treated with distilled water. Leaves were extracted in 1:1 methanol:water and then analysed, using an in-house GC-MS untargeted workflow.
    Results: Results identified 199 metabolites with only 6 of them (cis-aconitic acid, allantoin, cellobiose, glyceric acid, maltose and octadecanoic acid) found to be statistically significant (p < 0.05) between the HR and wild type buffalo grass varieties compared to the control experiment. Among these metabolites, unusual accumulation of allantoin was prominent and was an unanticipated effect of the pat gene insertion. As expected, glufosinate treatment caused significant metabolic alterations in the sensitive wild type, with the up-regulation of several amino acids (e.g. phenylalanine and isoleucine) which was likely due to glufosinate-induced senescence. The aminoacyl-tRNA biosynthetic pathway was identified as the most significant enriched pathway as a result of glufosinate effects because a number of its intermediates were amino acids.
    Conclusion: HR buffalo grasses were very similar to its wild type comparator based on a comprehensive GC-MS based untargeted metabolomics and therefore, should guarantee the safe use of these HR buffalo grasses. The current metabolomics analyses not only confirmed the effects of glufosinate to up-regulate free amino acid pools in the sensitive wild type but also several alterations in sugar, sugar phosphate and organic acid metabolism have been reported.
    MeSH term(s) Aminobutyrates/metabolism ; Aminobutyrates/pharmacology ; Animals ; Buffaloes ; Delayed-Action Preparations/metabolism ; Gas Chromatography-Mass Spectrometry ; Herbicide Resistance/genetics ; Herbicides/metabolism ; Herbicides/pharmacology ; Metabolomics ; Poaceae/drug effects ; Poaceae/genetics ; Poaceae/metabolism
    Chemical Substances Aminobutyrates ; Delayed-Action Preparations ; Herbicides ; phosphinothricin (51276-47-2)
    Language English
    Publishing date 2020-01-27
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2250617-2
    ISSN 1573-3890 ; 1573-3882
    ISSN (online) 1573-3890
    ISSN 1573-3882
    DOI 10.1007/s11306-020-1644-9
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  5. Article ; Online: LC-MS untargeted metabolomics assesses the delayed response of glufosinate treatment of transgenic glufosinate resistant (GR) buffalo grasses (Stenotaphrum secundatum L.).

    Boonchaisri, Siriwat / Rochfort, Simone / Stevenson, Trevor / Dias, Daniel A

    Metabolomics : Official journal of the Metabolomic Society

    2021  Volume 17, Issue 3, Page(s) 28

    Abstract: Introduction: Glufosinate resistant (GR) buffalo grasses were genetically modified to resist the broad-spectrum herbicide, glufosinate by inserting a novel pat gene into its genome. This modification results in a production of additional ... ...

    Abstract Introduction: Glufosinate resistant (GR) buffalo grasses were genetically modified to resist the broad-spectrum herbicide, glufosinate by inserting a novel pat gene into its genome. This modification results in a production of additional phosphinothricin acetyltransferase (PAT) to detoxify the deleterious effects of glufosinate. The GR grasses and its associated herbicide form a modern, weeding program, to eradicate obnoxious weeds in turf lawn without damaging the grasses at relatively low costs and labor. As with several principal crops which are genetically modified to improve agricultural traits, biosafety of the GR buffalo grasses is inevitably expected to become a public concern. For the first time, we had previously examined the metabolome of glufosinate-resistant buffalo grasses, using a GC-MS untargeted approach to assess the risk of GR as well as identify any pleotropic effects arising from the genetically modification process. In this paper, an untargeted high-resolution LC-MS (LC-HRMS) untargeted metabolomics approach was carried out to complement our previous findings with respect to GR and wild type (WT) buffalo grasses.
    Objective: One of the major aims of this present work was to compare GR to WT buffalo grasses by including the detection of the secondary metabolome and determine any unprecedented metabolic changes.
    Methods: Eight-week old plants of 4 GR buffalo grasses, (93-1A, 93-2B, 93-3 C and 93-5A) and 3 wild type varieties (WT 8-4A, WT 9-1B and WT 9-1B) were submerged in either 5 % v/v of glufosinate or distilled water 3 days prior to a LC-HRMS based untargeted metabolomics analysis (glufosinate-treated or control, samples, respectively). An Ultra-High-Performance Liquid Chromatography (UHPLC) system coupled to a Velos Pro Orbitrap mass spectrometer system was employed to holistically measure the primary and secondary metabolome of both GR and WT buffalo grasses either treated with or without glufosinate and subsequently apply several bioinformatic tools including the automated pathway analysis algorithm, mummichog.
    Results: LC-HRMS untargeted based metabolomics clearly identified that the global metabolite pools of both GR and WT cultivars were highly similar, providing strong, supporting evidence of substantial equivalence between the GR and WT varieties. These findings indicate that if any associated risks to these GR grasses were somehow present, the risk would be within those acceptable ranges present in the WT. Additionally, mummichog-based pathway analysis indicated that phenylalanine metabolism and the TCA cycle were significantly impacted by glufosinate treatment in the WT cultivar. It was possible that alterations in the relative concentrations of several intermediates in these pathways were likely due to glufosinate-induced production of secondary metabolites to enhance plant defense mechanisms against herbicidal stress at the expense of primary metabolism.
    Conclusions: GR buffalo grasses were found to be near identical to its WT comparator based on this complementary LC-HRMS based untargeted metabolomics. Therefore, these results further support the safe use of these GR buffalo grasses with substantial evidence. Interestingly, despite protected by PAT, GR buffalo grasses still demonstrated the response to glufosinate treatment by up-regulating some secondary metabolite-related pathways.
    MeSH term(s) Agriculture ; Aminobutyrates/pharmacology ; Animals ; Buffaloes/metabolism ; Chromatography, High Pressure Liquid ; Chromatography, Liquid/methods ; Crops, Agricultural/metabolism ; Gas Chromatography-Mass Spectrometry ; Herbicide Resistance/genetics ; Herbicides/metabolism ; Herbicides/pharmacology ; Metabolome ; Metabolomics/methods ; Plant Weeds/metabolism ; Poaceae/metabolism ; Tandem Mass Spectrometry/methods
    Chemical Substances Aminobutyrates ; Herbicides ; phosphinothricin (51276-47-2)
    Language English
    Publishing date 2021-02-20
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2250617-2
    ISSN 1573-3890 ; 1573-3882
    ISSN (online) 1573-3890
    ISSN 1573-3882
    DOI 10.1007/s11306-021-01776-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Understanding glycaemic control and current approaches for screening antidiabetic natural products from evidence-based medicinal plants.

    Lankatillake, Chintha / Huynh, Tien / Dias, Daniel A

    Plant methods

    2019  Volume 15, Page(s) 105

    Abstract: Type 2 Diabetes Mellitus has reached epidemic proportions as a result of over-nutrition and increasingly sedentary lifestyles. Current therapies, although effective, are not without limitations. These limitations, the alarming increase in the prevalence ... ...

    Abstract Type 2 Diabetes Mellitus has reached epidemic proportions as a result of over-nutrition and increasingly sedentary lifestyles. Current therapies, although effective, are not without limitations. These limitations, the alarming increase in the prevalence of diabetes, and the soaring cost of managing diabetes and its complications underscores an urgent need for safer, more efficient and affordable alternative treatments. Over 1200 plant species are reported in ethnomedicine for treating diabetes and these represents an important and promising source for the identification of novel antidiabetic compounds. Evaluating medicinal plants for desirable bioactivity goes hand-in-hand with methods in analytical biochemistry for separating and identifying lead compounds. This review aims to provide a comprehensive summary of current methods used in antidiabetic plant research to form a useful resource for researchers beginning in the field. The review summarises the current understanding of blood glucose regulation and the general mechanisms of action of current antidiabetic medications, and combines knowledge on common experimental approaches for screening plant extracts for antidiabetic activity and currently available analytical methods and technologies for the separation and identification of bioactive natural products. Common in vivo animal models, in vitro models, in silico methods and biochemical assays used for testing the antidiabetic effects of plants are discussed with a particular emphasis on in vitro methods such as cell-based bioassays for screening insulin secretagogues and insulinomimetics. Enzyme inhibition assays and molecular docking are also highlighted. The role of metabolomics, metabolite profiling, and dereplication of data for the high-throughput discovery of novel antidiabetic agents is reviewed. Finally, this review also summarises sample preparation techniques such as liquid-liquid extraction, solid phase extraction, and supercritical fluid extraction, and the critical function of nuclear magnetic resonance and high resolution liquid chromatography-mass spectrometry for the dereplication, putative identification and structure elucidation of natural compounds from evidence-based medicinal plants.
    Language English
    Publishing date 2019-09-07
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2203723-8
    ISSN 1746-4811
    ISSN 1746-4811
    DOI 10.1186/s13007-019-0487-8
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  7. Article ; Online: Dead in the water - Role of relic DNA and primer choice for targeted sequencing surveys of anaerobic sewage sludge intended for biological monitoring.

    Krohn, Christian / Jansriphibul, Kraiwut / Dias, Daniel A / Rees, Catherine A / Akker, Ben van den / Boer, Jennifer C / Plebanski, Magdalena / Surapaneni, Aravind / O'Carroll, Denis / Richard, Stuetz / Batstone, Damien J / Ball, Andrew S

    Water research

    2024  Volume 253, Page(s) 121354

    Abstract: DNA-based monitoring of microbial communities that are responsible for the performance of anaerobic digestion of sewage wastes has the potential to improve resource recoveries for wastewater treatment facilities. By treating sludge with propidium ... ...

    Abstract DNA-based monitoring of microbial communities that are responsible for the performance of anaerobic digestion of sewage wastes has the potential to improve resource recoveries for wastewater treatment facilities. By treating sludge with propidium monoazide (PMA) prior to amplicon sequencing, this study explored how the presence of DNA from dead microbial biomass carried over with feed sludge may mislead process-relevant biomarkers, and whether primer choice impacts such assessments. Four common primers were selected for amplicon preparation, also to determine if universal primers have sufficient taxonomic or functional coverage for monitoring ecological performance; or whether two domain-specific primers for Bacteria and Archaea are necessary. Anaerobic sludges of three municipal continuously stirred-tank reactors in Victoria, Australia, were sampled at one time-point. A total of 240 amplicon libraries were sequenced on a Miseq using two universal and two domain-specific primer pairs. Untargeted metabolomics was chosen to complement biological interpretation of amplicon gene-based functional predictions. Diversity, taxonomy, phylogeny and functional potentials were systematically assessed using PICRUSt2, which can predict community wide pathway abundance. The two chosen universal primers provided similar diversity profiles of abundant Bacteria and Archaea, compared to the domain-specific primers. About 16 % of all detected prokaryotic genera covering 30 % of total abundances and 6 % of PICRUSt2-estimated pathway abundances were affected by PMA. This showed that dead biomass in the anaerobic digesters impacted DNA-based assessments, with implications for predicting active processes, such as methanogenesis, denitrification or the identification of organisms associated with biological foams. Hence, instead of running two sequencing runs with two different domain-specific primers, we propose conducting PMA-seq with universal primer pairs for routine performance monitoring. However, dead sludge biomass may have some predictive value. In principal component analysis the compositional variation of 239 sludge metabolites resembled that of 'dead-plus-alive' biomass, suggesting that dead organisms contributed to the potentially process-relevant sludge metabolome.
    MeSH term(s) Sewage/microbiology ; Anaerobiosis ; Biological Monitoring ; Bacteria/metabolism ; Archaea/metabolism ; DNA/metabolism ; Victoria ; Bioreactors/microbiology ; Methane/metabolism ; RNA, Ribosomal, 16S/genetics ; RNA, Ribosomal, 16S/metabolism
    Chemical Substances Sewage ; DNA (9007-49-2) ; Methane (OP0UW79H66) ; RNA, Ribosomal, 16S
    Language English
    Publishing date 2024-02-22
    Publishing country England
    Document type Journal Article
    ZDB-ID 202613-2
    ISSN 1879-2448 ; 0043-1354
    ISSN (online) 1879-2448
    ISSN 0043-1354
    DOI 10.1016/j.watres.2024.121354
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Understanding glycaemic control and current approaches for screening antidiabetic natural products from evidence-based medicinal plants

    Lankatillake, Chintha / Huynh, Tien / Dias, Daniel A

    Plant methods. 2019 Dec., v. 15, no. 1

    2019  

    Abstract: Type 2 Diabetes Mellitus has reached epidemic proportions as a result of over-nutrition and increasingly sedentary lifestyles. Current therapies, although effective, are not without limitations. These limitations, the alarming increase in the prevalence ... ...

    Abstract Type 2 Diabetes Mellitus has reached epidemic proportions as a result of over-nutrition and increasingly sedentary lifestyles. Current therapies, although effective, are not without limitations. These limitations, the alarming increase in the prevalence of diabetes, and the soaring cost of managing diabetes and its complications underscores an urgent need for safer, more efficient and affordable alternative treatments. Over 1200 plant species are reported in ethnomedicine for treating diabetes and these represents an important and promising source for the identification of novel antidiabetic compounds. Evaluating medicinal plants for desirable bioactivity goes hand-in-hand with methods in analytical biochemistry for separating and identifying lead compounds. This review aims to provide a comprehensive summary of current methods used in antidiabetic plant research to form a useful resource for researchers beginning in the field. The review summarises the current understanding of blood glucose regulation and the general mechanisms of action of current antidiabetic medications, and combines knowledge on common experimental approaches for screening plant extracts for antidiabetic activity and currently available analytical methods and technologies for the separation and identification of bioactive natural products. Common in vivo animal models, in vitro models, in silico methods and biochemical assays used for testing the antidiabetic effects of plants are discussed with a particular emphasis on in vitro methods such as cell-based bioassays for screening insulin secretagogues and insulinomimetics. Enzyme inhibition assays and molecular docking are also highlighted. The role of metabolomics, metabolite profiling, and dereplication of data for the high-throughput discovery of novel antidiabetic agents is reviewed. Finally, this review also summarises sample preparation techniques such as liquid–liquid extraction, solid phase extraction, and supercritical fluid extraction, and the critical function of nuclear magnetic resonance and high resolution liquid chromatography–mass spectrometry for the dereplication, putative identification and structure elucidation of natural compounds from evidence-based medicinal plants.
    Keywords animal models ; bioassays ; blood glucose ; computer simulation ; enzyme inhibition ; glycemic control ; glycemic effect ; hypoglycemic agents ; in vitro studies ; insulin ; lifestyle ; liquid chromatography ; liquid-liquid extraction ; mass spectrometry ; mechanism of action ; medicinal plants ; metabolites ; metabolomics ; noninsulin-dependent diabetes mellitus ; nuclear magnetic resonance spectroscopy ; plant extracts ; screening ; solid phase extraction ; supercritical fluid extraction ; traditional medicine
    Language English
    Dates of publication 2019-12
    Size p. 105.
    Publishing place BioMed Central
    Document type Article
    Note Review
    ISSN 1746-4811
    DOI 10.1186/s13007-019-0487-8
    Database NAL-Catalogue (AGRICOLA)

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  9. Article: Biomarkers associated with cheese quality uncovered by integrative multi-omic analysis

    Afshari, Roya / Pillidge, Christopher J / Dias, Daniel A / Osborn, A. Mark / Gill, Harsharn

    Food control. 2021 May, v. 123

    2021  

    Abstract: Cheese quality is determined by many dynamic complex interactions that occur between cheese microbiota, metabolites and milk substrates. Here we report that application of multi-omics and data integration (by Data Integration Analysis for Biomarker ... ...

    Abstract Cheese quality is determined by many dynamic complex interactions that occur between cheese microbiota, metabolites and milk substrates. Here we report that application of multi-omics and data integration (by Data Integration Analysis for Biomarker discovery using Latent components, DIABLO) is able to identify and rank biomarkers (taxa and metabolites) which could discriminate, at the molecular level, cheddar cheeses of different quality made by the same manufacturer and to the same specifications. Samples of high-quality mature cheddar had higher amounts of proline, histidine, isoleucine and aspartic acid but lower amounts of stearic acid and octadecanol relative to the lower-quality cheddar. Furthermore, Streptococcus species (presumably S. thermophilus) were present in higher abundance relative to Lactococcus lactis in the higher-quality cheddar. Integrative analysis also revealed significant relationships between these multi-omics biomarkers. Together these results highlight the potential for this approach for identifying biomarkers that could be used to discriminate cheeses of varying quality and as a complementary approach to the current manual sensory evaluation of cheese.
    Keywords Cheddar cheese ; Lactococcus lactis ; Streptococcus ; aspartic acid ; biomarkers ; cheese quality ; food safety ; histidine ; isoleucine ; metabolites ; microorganisms ; milk ; multiomics ; proline ; sampling ; sensory evaluation ; stearic acid
    Language English
    Dates of publication 2021-05
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 1027805-9
    ISSN 0956-7135
    ISSN 0956-7135
    DOI 10.1016/j.foodcont.2020.107752
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  10. Article: Progress in Metabolomics Standardisation and its Significance in Future Clinical Laboratory Medicine.

    Dias, Daniel A / Koal, Therese

    EJIFCC

    2016  Volume 27, Issue 4, Page(s) 331–343

    Abstract: Today, the technology of 'targeted' based metabolomics is pivotal in the clinical analysis workflow as it provides information of metabolic phenotyping (metabotypes) by enhancing our understanding of metabolism of complex diseases, biomarker discovery ... ...

    Abstract Today, the technology of 'targeted' based metabolomics is pivotal in the clinical analysis workflow as it provides information of metabolic phenotyping (metabotypes) by enhancing our understanding of metabolism of complex diseases, biomarker discovery for disease development, progression, treatment, and drug function and assessment. This review is focused on surveying and providing a gap analysis on metabolic phenotyping with a focus on targeted based metabolomics from an instrumental, technical
    Language English
    Publishing date 2016-12-01
    Publishing country Italy
    Document type Journal Article
    ISSN 1650-3414
    ISSN 1650-3414
    Database MEDical Literature Analysis and Retrieval System OnLINE

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