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  1. Article ; Online: Lemon gum: Non-toxic arabinogalactan isolated from Citrus × latifolia with antiproliferative property against human prostate adenocarcinoma cells.

    Ribeiro, Fábio de Oliveira Silva / Oliveira, Fátima de Cássia Evangelista de / Pessoa, Claudia / Dias, Jhones do Nascimento / Albuquerque, Patrícia / Sousa, Edymilaís da Silva / Lima, Sidney Gonçalo de / Lima, Laís Ramos Monteiro de / Sombra, Venicios G / Paula, Regina Célia Monteiro de / Alves, Even Herlany Pereira / Vasconcelos, Daniel Fernando Pereira / Fontenele, Darllan Damasceno / Iles, Bruno / Medeiros, Jand Venes Rolim / Araújo, Alyne Rodrigues de / da Silva, Durcilene Alves / Leite, José Roberto de Souza de Almeida

    International journal of biological macromolecules

    2023  Volume 232, Page(s) 123058

    Abstract: Lemon gum (LG) obtained from Citrus × latifolia in Brazil was isolated and characterized. In addition, gum biocompatibility was evaluated in vitro and in vivo by Galleria mellonella and mice model. The cytotoxicity against tumor cells was also evaluated. ...

    Abstract Lemon gum (LG) obtained from Citrus × latifolia in Brazil was isolated and characterized. In addition, gum biocompatibility was evaluated in vitro and in vivo by Galleria mellonella and mice model. The cytotoxicity against tumor cells was also evaluated. The ratio of arabinose:galactose: rhamnose:4-OMe-glucuronic acid was 1:0.65:0.06:0.15. Small traces of protein were detected, emphasizing the isolate purity. Molar mass was 8.08 × 10
    MeSH term(s) Male ; Animals ; Mice ; Humans ; Citrus ; Prostate ; Galactans ; Adenocarcinoma/drug therapy
    Chemical Substances arabinogalactan (SL4SX1O487) ; Galactans
    Language English
    Publishing date 2023-01-18
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2022.12.277
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: The Antioxidant Peptide Salamandrin-I: First Bioactive Peptide Identified from Skin Secretion of Salamandra Genus (Salamandra salamandra).

    Plácido, Alexandra / Bueno, João / Barbosa, Eder A / Moreira, Daniel C / Dias, Jhones do Nascimento / Cabral, Wanessa Felix / Albuquerque, Patrícia / Bessa, Lucinda J / Freitas, Jaime / Kuckelhaus, Selma A S / Lima, Filipe C D A / Batagin-Neto, Augusto / Brand, Guilherme D / Relvas, João B / Leite, José Roberto S A / Eaton, Peter

    Biomolecules

    2020  Volume 10, Issue 4

    Abstract: Amphibian skin is a multifunctional organ that plays key roles in defense, breathing, and water balance. In this study, skin secretion samples of the fire salamander (Salamandra salamandra) were separated using RP-HPLC and de novo sequenced using MALDI- ... ...

    Abstract Amphibian skin is a multifunctional organ that plays key roles in defense, breathing, and water balance. In this study, skin secretion samples of the fire salamander (Salamandra salamandra) were separated using RP-HPLC and de novo sequenced using MALDI-TOF MS/MS. Next, we used an in silico platform to screen antioxidant molecules in the framework of density functional theory. One of the identified peptides, salamandrin-I, [M + H]
    MeSH term(s) Amphibian Proteins/chemistry ; Amphibian Proteins/isolation & purification ; Amphibian Proteins/pharmacology ; Animals ; Anti-Bacterial Agents/chemistry ; Anti-Bacterial Agents/pharmacology ; Antioxidants/chemistry ; Antioxidants/pharmacology ; Circular Dichroism ; Drug Evaluation, Preclinical ; Humans ; Microbial Sensitivity Tests ; Moths/drug effects ; Peptides/chemistry ; Peptides/pharmacology ; Salamandra ; Skin/chemistry ; Toxicity Tests
    Chemical Substances Amphibian Proteins ; Anti-Bacterial Agents ; Antioxidants ; Peptides
    Language English
    Publishing date 2020-03-27
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2701262-1
    ISSN 2218-273X ; 2218-273X
    ISSN (online) 2218-273X
    ISSN 2218-273X
    DOI 10.3390/biom10040512
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Antifungal and anti-inflammatory potential of eschweilenol C-rich fraction derived from Terminalia fagifolia Mart

    Rodrigues de Araújo, Alyne / Iles, Bruno / de Melo Nogueira, Kerolayne / Dias, Jhones do Nascimento / Plácido, Alexandra / Rodrigues, Artur / Albuquerque, Patrícia / Silva-Pereira, Ildinete / Socodatto, Renato / Portugal, Camila C / Relvas, João B / Costa Véras, Leiz Maria / Dalmatti Alves Lima, Filipe Camargo / Batagin-Neto, Augusto / Rolim Medeiros, Jand-Venes / Moreira Nunes, Paulo Humberto / Eaton, Peter / de Souza de Almeida Leite, José Roberto

    Journal of ethnopharmacology. 2019 Aug. 10, v. 240

    2019  

    Abstract: Folk knowledge transmitted between generations allows traditional populations to maintain the use of medicinal plants for the treatment of several diseases. In this context, the species Terminalia fagifolia Mart., native to Brazil, is used for the ... ...

    Abstract Folk knowledge transmitted between generations allows traditional populations to maintain the use of medicinal plants for the treatment of several diseases. In this context, the species Terminalia fagifolia Mart., native to Brazil, is used for the treatment of chronic and infectious diseases. Plants rich in secondary metabolites, such as this species and their derivatives, may represent therapeutic alternatives for the treatment of diseases that reduce the quality of life of people.The aim of this study was to evaluate the antifungal and anti-inflammatory potential of aqueous fraction from ethanolic extract of T. fagifolia, with in silico study of the major compound of the fraction.The phytochemical study of the aqueous fraction was performed by HPLC, LC/MS and NMR. The antifungal activity was evaluated against yeasts, by determination of the minimum inhibitory concentration and minimum fungicidal concentration. The effect on Candida albicans was analyzed by AFM. The antibiofilm potential against biofilms of C. albicans was also tested. The anti-inflammatory potential of the aqueous fraction was evaluated in vivo by the carrageenan-induced paw edema and peritonitis. A microglial model of LPS-induced neuroinflammation was also studied. Further insights on the activation mechanism were studied using quantum chemistry computer simulations. Toxicity was evaluated in the Galleria mellonella and human erythrocytes models.Eschweilenol C was identified as the major constituent of the aqueous fraction of the ethanolic extract of T. fagifolia. The aqueous fraction was active against all Candida strains used (sensitive and resistant to Fluconazole) with MICs ranging from 1000 to 0.4 μg/mL. By AFM it was possible to observe morphological alterations in treated Candida cells. The fraction significantly (p < 0.05) inhibited paw edema and decreased levels of malondialdehyde induced by carrageenan. In a microglial cell model, aqueous fraction demonstrated the ability to inhibit NF-κB after induction with lipopolysaccharide. The theoretical studies showed structural similarity between eschweilenol C and indomethacin and an excellent antioxidant potential. The aqueous fraction did not present toxicity in the studied models.The results indicate that the aqueous fraction of T. fagifolia has potential for biomedical applications with low toxicity. This finding can be attributed to the predominance of eschweilenol C in the aqueous fraction.
    Keywords Candida albicans ; Galleria mellonella ; Terminalia ; anti-inflammatory activity ; antifungal properties ; antioxidant activity ; biofilm ; carrageenan ; computer simulation ; edema ; erythrocytes ; fluconazole ; high performance liquid chromatography ; humans ; indomethacin ; infectious diseases ; lipopolysaccharides ; malondialdehyde ; medicinal plants ; minimum inhibitory concentration ; models ; neuroglia ; nuclear magnetic resonance spectroscopy ; peritonitis ; phytochemicals ; quality of life ; secondary metabolites ; therapeutics ; toxicity ; traditional medicine ; transcription factor NF-kappa B ; yeasts ; Brazil
    Language English
    Dates of publication 2019-0810
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 134511-4
    ISSN 1872-7573 ; 0378-8741
    ISSN (online) 1872-7573
    ISSN 0378-8741
    DOI 10.1016/j.jep.2019.111941
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 90/710: Show issues

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  4. Article ; Online: Antifungal and anti-inflammatory potential of eschweilenol C-rich fraction derived from Terminalia fagifolia Mart.

    Rodrigues de Araújo, Alyne / Iles, Bruno / de Melo Nogueira, Kerolayne / Dias, Jhones do Nascimento / Plácido, Alexandra / Rodrigues, Artur / Albuquerque, Patrícia / Silva-Pereira, Ildinete / Socodatto, Renato / Portugal, Camila C / Relvas, João B / Costa Véras, Leiz Maria / Dalmatti Alves Lima, Filipe Camargo / Batagin-Neto, Augusto / Rolim Medeiros, Jand-Venes / Moreira Nunes, Paulo Humberto / Eaton, Peter / de Souza de Almeida Leite, José Roberto

    Journal of ethnopharmacology

    2019  Volume 240, Page(s) 111941

    Abstract: Ethnopharmacological relevance: Folk knowledge transmitted between generations allows traditional populations to maintain the use of medicinal plants for the treatment of several diseases. In this context, the species Terminalia fagifolia Mart., native ... ...

    Abstract Ethnopharmacological relevance: Folk knowledge transmitted between generations allows traditional populations to maintain the use of medicinal plants for the treatment of several diseases. In this context, the species Terminalia fagifolia Mart., native to Brazil, is used for the treatment of chronic and infectious diseases. Plants rich in secondary metabolites, such as this species and their derivatives, may represent therapeutic alternatives for the treatment of diseases that reduce the quality of life of people.
    Aim of the study: The aim of this study was to evaluate the antifungal and anti-inflammatory potential of aqueous fraction from ethanolic extract of T. fagifolia, with in silico study of the major compound of the fraction.
    Material and methods: The phytochemical study of the aqueous fraction was performed by HPLC, LC/MS and NMR. The antifungal activity was evaluated against yeasts, by determination of the minimum inhibitory concentration and minimum fungicidal concentration. The effect on Candida albicans was analyzed by AFM. The antibiofilm potential against biofilms of C. albicans was also tested. The anti-inflammatory potential of the aqueous fraction was evaluated in vivo by the carrageenan-induced paw edema and peritonitis. A microglial model of LPS-induced neuroinflammation was also studied. Further insights on the activation mechanism were studied using quantum chemistry computer simulations. Toxicity was evaluated in the Galleria mellonella and human erythrocytes models.
    Results: Eschweilenol C was identified as the major constituent of the aqueous fraction of the ethanolic extract of T. fagifolia. The aqueous fraction was active against all Candida strains used (sensitive and resistant to Fluconazole) with MICs ranging from 1000 to 0.4 μg/mL. By AFM it was possible to observe morphological alterations in treated Candida cells. The fraction significantly (p < 0.05) inhibited paw edema and decreased levels of malondialdehyde induced by carrageenan. In a microglial cell model, aqueous fraction demonstrated the ability to inhibit NF-κB after induction with lipopolysaccharide. The theoretical studies showed structural similarity between eschweilenol C and indomethacin and an excellent antioxidant potential. The aqueous fraction did not present toxicity in the studied models.
    Conclusion: The results indicate that the aqueous fraction of T. fagifolia has potential for biomedical applications with low toxicity. This finding can be attributed to the predominance of eschweilenol C in the aqueous fraction.
    MeSH term(s) Animals ; Anti-Inflammatory Agents/pharmacology ; Anti-Inflammatory Agents/therapeutic use ; Antifungal Agents/pharmacology ; Antifungal Agents/therapeutic use ; Candida albicans/drug effects ; Candida albicans/growth & development ; Carrageenan ; Cryptococcus neoformans/drug effects ; Cryptococcus neoformans/growth & development ; Edema/chemically induced ; Edema/drug therapy ; Ellagic Acid/pharmacology ; Ellagic Acid/therapeutic use ; Erythrocytes/drug effects ; Female ; Heterocyclic Compounds, 4 or More Rings/pharmacology ; Heterocyclic Compounds, 4 or More Rings/therapeutic use ; Humans ; Male ; Mice ; Microbial Sensitivity Tests ; Microglia/drug effects ; Microglia/metabolism ; NF-kappa B/metabolism ; Plant Extracts/pharmacology ; Plant Extracts/therapeutic use ; Terminalia
    Chemical Substances Anti-Inflammatory Agents ; Antifungal Agents ; Heterocyclic Compounds, 4 or More Rings ; NF-kappa B ; Plant Extracts ; eschweilenol C ; Ellagic Acid (19YRN3ZS9P) ; Carrageenan (9000-07-1)
    Language English
    Publishing date 2019-05-15
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 134511-4
    ISSN 1872-7573 ; 0378-8741
    ISSN (online) 1872-7573
    ISSN 0378-8741
    DOI 10.1016/j.jep.2019.111941
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1494: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  5. Article ; Online: A hidden battle in the dirt: Soil amoebae interactions with Paracoccidioides spp.

    Albuquerque, Patrícia / Nicola, André Moraes / Magnabosco, Diogo Almeida Gomes / Derengowski, Lorena da Silveira / Crisóstomo, Luana Soares / Xavier, Luciano Costa Gomes / Frazão, Stefânia de Oliveira / Guilhelmelli, Fernanda / de Oliveira, Marco Antônio / Dias, Jhones do Nascimento / Hurtado, Fabián Andrés / Teixeira, Marcus de Melo / Guimarães, Allan Jefferson / Paes, Hugo Costa / Bagagli, Eduardo / Felipe, Maria Sueli Soares / Casadevall, Arturo / Silva-Pereira, Ildinete

    PLoS neglected tropical diseases

    2019  Volume 13, Issue 10, Page(s) e0007742

    Abstract: Paracoccidioides spp. are thermodimorphic fungi that cause a neglected tropical disease (paracoccidioidomycosis) that is endemic to Latin America. These fungi inhabit the soil, where they live as saprophytes with no need for a mammalian host to complete ... ...

    Abstract Paracoccidioides spp. are thermodimorphic fungi that cause a neglected tropical disease (paracoccidioidomycosis) that is endemic to Latin America. These fungi inhabit the soil, where they live as saprophytes with no need for a mammalian host to complete their life cycle. Despite this, they developed sophisticated virulence attributes allowing them not only to survive in host tissues but also to cause disease. A hypothesis for selective pressures driving the emergence or maintenance of virulence of soil fungi is their interaction with soil predators such as amoebae and helminths. We evaluated the presence of environmental amoeboid predators in soil from armadillo burrows where Paracoccidioides had been previously detected and tested if the interaction of Paracoccidioides with amoebae selects for fungi with increased virulence. Nematodes, ciliates, and amoebae-all potential predators of fungi-grew in cultures from soil samples. Microscopical observation and ITS sequencing identified the amoebae as Acanthamoeba spp, Allovahlkampfia spelaea, and Vermamoeba vermiformis. These three amoebae efficiently ingested, killed and digested Paracoccidioides spp. yeast cells, as did laboratory adapted axenic Acanthamoeba castellanii. Sequential co-cultivation of Paracoccidioides with A. castellanii selected for phenotypical traits related to the survival of the fungus within a natural predator as well as in murine macrophages and in vivo (Galleria mellonella and mice). These changes in virulence were linked to the accumulation of cell wall alpha-glucans, polysaccharides that mask recognition of fungal molecular patterns by host pattern recognition receptors. Altogether, our results indicate that Paracoccidioides inhabits a complex environment with multiple amoeboid predators that can exert selective pressure to guide the evolution of virulence traits.
    MeSH term(s) Acanthamoeba castellanii/physiology ; Amoeba/cytology ; Amoeba/microbiology ; Amoeba/physiology ; Animals ; Armadillos ; Ciliophora ; Coculture Techniques ; Disease Models, Animal ; Fungi ; Host-Pathogen Interactions/physiology ; Macrophages/microbiology ; Male ; Mice ; Mice, Inbred BALB C ; Nematoda ; Paracoccidioides/pathogenicity ; Paracoccidioides/physiology ; Paracoccidioidomycosis/microbiology ; Phagocytosis ; Soil ; Soil Microbiology ; Virulence ; Virulence Factors/physiology
    Chemical Substances Soil ; Virulence Factors
    Language English
    Publishing date 2019-10-07
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2429704-5
    ISSN 1935-2735 ; 1935-2727
    ISSN (online) 1935-2735
    ISSN 1935-2727
    DOI 10.1371/journal.pntd.0007742
    Database MEDical Literature Analysis and Retrieval System OnLINE

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