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Article ; Online: Isolation and Maintenance of In Vitro Cell Cultures from the Ctenophore Mnemiopsis leidyi.

Dieter, Abigail C / Vandepas, Lauren E / Browne, William E

Methods in molecular biology (Clifton, N.J.)

2022  Volume 2450, Page(s) 347–358

Abstract: The ability to isolate, monitor, and examine specific cells of interest enables targeted experimental manipulations that would otherwise be difficult to perform and interpret in the context of the whole organism. In vitro primary cell cultures derived ... ...

Abstract The ability to isolate, monitor, and examine specific cells of interest enables targeted experimental manipulations that would otherwise be difficult to perform and interpret in the context of the whole organism. In vitro primary cell cultures derived from ctenophores thus serve as an important tool for understanding complex cellular and molecular interactions that take place both within and between various ctenophore cell types. Here we describe methods for reliably generating and maintaining primary cell cultures derived from the lobate ctenophore Mnemiopsis leidyi that can be used for a wide variety of experimental applications.
MeSH term(s) Animals ; Cell Culture Techniques ; Ctenophora
Language English
Publishing date 2022-04-28
Publishing country United States
Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, Non-U.S. Gov't
ISSN 1940-6029
ISSN (online) 1940-6029
DOI 10.1007/978-1-0716-2172-1_18
Database MEDical Literature Analysis and Retrieval System OnLINE

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