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  1. Article ; Online: Gradients and consequences of heterogeneity in biofilms.

    Jo, Jeanyoung / Price-Whelan, Alexa / Dietrich, Lars E P

    Nature reviews. Microbiology

    2022  Volume 20, Issue 10, Page(s) 593–607

    Abstract: Historically, appreciation for the roles of resource gradients in biology has fluctuated inversely to the popularity of genetic mechanisms. Nevertheless, in microbiology specifically, widespread recognition of the multicellular lifestyle has recently ... ...

    Abstract Historically, appreciation for the roles of resource gradients in biology has fluctuated inversely to the popularity of genetic mechanisms. Nevertheless, in microbiology specifically, widespread recognition of the multicellular lifestyle has recently brought new emphasis to the importance of resource gradients. Most microorganisms grow in assemblages such as biofilms or spatially constrained communities with gradients that influence, and are influenced by, metabolism. In this Review, we discuss examples of gradient formation and physiological differentiation in microbial assemblages growing in diverse settings. We highlight consequences of physiological heterogeneity in microbial assemblages, including division of labour and increased resistance to stress. Our impressions of microbial behaviour in various ecosystems are not complete without complementary maps of the chemical and physical geographies that influence cellular activities. A holistic view, incorporating these geographies and the genetically encoded functions that operate within them, will be essential for understanding microbial assemblages in their many roles and potential applications.
    MeSH term(s) Biofilms ; Ecosystem
    Language English
    Publishing date 2022-02-11
    Publishing country England
    Document type Journal Article ; Review ; Research Support, N.I.H., Extramural
    ZDB-ID 2139054-X
    ISSN 1740-1534 ; 1740-1526
    ISSN (online) 1740-1534
    ISSN 1740-1526
    DOI 10.1038/s41579-022-00692-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5865: Show issues Location:
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  2. Article: Cyanide-dependent control of terminal oxidase hybridization by

    Smiley, Marina K / Sekaran, Doran C / Price-Whelan, Alexa / Dietrich, Lars E P

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Pseudomonas ... ...

    Abstract Pseudomonas aeruginosa
    Language English
    Publishing date 2023-06-20
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.05.31.543164
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Light/Dark and Temperature Cycling Modulate Metabolic Electron Flow in Pseudomonas aeruginosa Biofilms.

    Kahl, Lisa Juliane / Eckartt, Kelly N / Morales, Diana K / Price-Whelan, Alexa / Dietrich, Lars E P

    mBio

    2022  Volume 13, Issue 4, Page(s) e0140722

    Abstract: Sunlight drives phototrophic metabolism, which affects redox conditions and produces substrates for nonphototrophs. These environmental parameters fluctuate daily due to Earth's rotation, and nonphototrophic organisms can therefore benefit from the ... ...

    Abstract Sunlight drives phototrophic metabolism, which affects redox conditions and produces substrates for nonphototrophs. These environmental parameters fluctuate daily due to Earth's rotation, and nonphototrophic organisms can therefore benefit from the ability to respond to, or even anticipate, such changes. Circadian rhythms, such as daily changes in body temperature, in host organisms can also affect local conditions for colonizing bacteria. Here, we investigated the effects of light/dark and temperature cycling on biofilms of the opportunistic pathogen Pseudomonas aeruginosa PA14. We grew biofilms in the presence of a respiratory indicator dye and found that enhanced dye reduction occurred in biofilm zones that formed during dark intervals and at lower temperatures. This pattern formation occurred with cycling of blue, red, or far-red light, and a screen of mutants representing potential sensory proteins identified two with defects in pattern formation, specifically under red light cycling. We also found that the physiological states of biofilm subzones formed under specific light and temperature conditions were retained during subsequent condition cycling. Light/dark and temperature cycling affected expression of genes involved in primary metabolic pathways and redox homeostasis, including those encoding electron transport chain components. Consistent with this, we found that
    MeSH term(s) Animals ; Biofilms ; Electrons ; Oxidation-Reduction ; Pseudomonas aeruginosa/metabolism ; Temperature
    Language English
    Publishing date 2022-08-08
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2557172-2
    ISSN 2150-7511 ; 2161-2129
    ISSN (online) 2150-7511
    ISSN 2161-2129
    DOI 10.1128/mbio.01407-22
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: MpaR-driven expression of an orphan terminal oxidase subunit supports

    Smiley, Marina K / Sekaran, Doran C / Forouhar, Farhad / Wolin, Erica / Jovanovic, Marko / Price-Whelan, Alexa / Dietrich, Lars E P

    mBio

    2023  Volume 15, Issue 1, Page(s) e0292623

    Abstract: Importance: Cyanide is an inhibitor of heme-copper oxidases, which are required for aerobic respiration in all eukaryotes and many prokaryotes. This fast-acting poison can arise from diverse sources, but mechanisms by which bacteria sense it are poorly ... ...

    Abstract Importance: Cyanide is an inhibitor of heme-copper oxidases, which are required for aerobic respiration in all eukaryotes and many prokaryotes. This fast-acting poison can arise from diverse sources, but mechanisms by which bacteria sense it are poorly understood. We investigated the regulatory response to cyanide in the pathogenic bacterium
    MeSH term(s) Pseudomonas aeruginosa/metabolism ; Oxidoreductases/genetics ; Oxidoreductases/metabolism ; Cyanides/metabolism ; Respiration ; Biofilms ; Heme/metabolism ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism
    Chemical Substances Oxidoreductases (EC 1.-) ; Cyanides ; Heme (42VZT0U6YR) ; Bacterial Proteins
    Language English
    Publishing date 2023-12-19
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2557172-2
    ISSN 2150-7511 ; 2161-2129
    ISSN (online) 2150-7511
    ISSN 2161-2129
    DOI 10.1128/mbio.02926-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Spatial heterogeneity in biofilm metabolism elicited by local control of phenazine methylation.

    Evans, Christopher R / Smiley, Marina K / Thio, Sean Asahara / Wei, Mian / Price-Whelan, Alexa / Min, Wei / Dietrich, Lars E P

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Within biofilms, gradients of electron acceptors such as oxygen stimulate the formation of physiological subpopulations. This heterogeneity can enable cross-feeding and promote drug resilience, features of the multicellular lifestyle that make biofilm- ... ...

    Abstract Within biofilms, gradients of electron acceptors such as oxygen stimulate the formation of physiological subpopulations. This heterogeneity can enable cross-feeding and promote drug resilience, features of the multicellular lifestyle that make biofilm-based infections difficult to treat. The pathogenic bacterium
    Language English
    Publishing date 2023-02-16
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.02.15.528762
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Charge Mapping of

    Rabinowitz, Jake / Hartel, Andreas J W / Dayton, Hannah / Fabbri, Jason D / Jo, Jeanyoung / Dietrich, Lars E P / Shepard, Kenneth L

    Analytical chemistry

    2023  Volume 95, Issue 12, Page(s) 5285–5292

    Abstract: Scanning ion conductance microscopy (SICM) is a topographic imaging technique capable of probing biological samples in electrolyte conditions. SICM enhancements have enabled surface charge detection based on voltage-dependent signals. Here, we show how ... ...

    Abstract Scanning ion conductance microscopy (SICM) is a topographic imaging technique capable of probing biological samples in electrolyte conditions. SICM enhancements have enabled surface charge detection based on voltage-dependent signals. Here, we show how the hopping mode SICM method (HP-SICM) can be used for rapid and minimally invasive surface charge mapping. We validate our method using
    MeSH term(s) Microscopy/methods ; Pseudomonas aeruginosa ; Radionuclide Imaging ; Ions ; Movement
    Chemical Substances Ions
    Language English
    Publishing date 2023-03-15
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, N.I.H., Extramural
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.2c05303
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4033: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
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  7. Article ; Online: Charge Mapping of Pseudomonas aeruginosa Using a Hopping Mode Scanning Ion Conductance Microscopy Technique

    Rabinowitz, Jake / Hartel, Andreas J. W. / Dayton, Hannah / Fabbri, Jason D. / Jo, Jeanyoung / Dietrich, Lars E. P. / Shepard, Kenneth L.

    Analytical Chemistry. 2023 Mar. 15, v. 95, no. 12 p.5285-5292

    2023  

    Abstract: Scanning ion conductance microscopy (SICM) is a topographic imaging technique capable of probing biological samples in electrolyte conditions. SICM enhancements have enabled surface charge detection based on voltage-dependent signals. Here, we show how ... ...

    Abstract Scanning ion conductance microscopy (SICM) is a topographic imaging technique capable of probing biological samples in electrolyte conditions. SICM enhancements have enabled surface charge detection based on voltage-dependent signals. Here, we show how the hopping mode SICM method (HP-SICM) can be used for rapid and minimally invasive surface charge mapping. We validate our method usingPseudomonas aeruginosaPA14 (PA) cells and observe a surface charge density of σPA = −2.0 ± 0.45 mC/m² that is homogeneous within the ∼80 nm lateral scan resolution. This biological surface charge is detected from at least 1.7 μm above the membrane (395× the Debye length), and the long-range charge detection is attributed to electroosmotic amplification. We show that imaging with a nanobubble-plugged probe reduces perturbation of the underlying sample. We extend the technique to PA biofilms and observe a charge density exceeding −20 mC/m². We use a solid-state calibration to quantify surface charge density and show that HP-SICM cannot be quantitatively described by a steady-state finite element model. This work contributes to the body of scanning probe methods that can uniquely contribute to microbiology and cellular biology.
    Keywords Pseudomonas aeruginosa ; analytical chemistry ; biofilm ; cell biology ; electrolytes ; electroosmosis ; finite element analysis ; microbiology ; microscopy ; models ; topography
    Language English
    Dates of publication 2023-0315
    Size p. 5285-5292.
    Publishing place American Chemical Society
    Document type Article ; Online
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.2c05303
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 4' 52/94: Show issues
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  8. Article ; Online: Cellular arrangement impacts metabolic activity and antibiotic tolerance in Pseudomonas aeruginosa biofilms.

    Dayton, Hannah / Kiss, Julie / Wei, Mian / Chauhan, Shradha / LaMarre, Emily / Cornell, William Cole / Morgan, Chase J / Janakiraman, Anuradha / Min, Wei / Tomer, Raju / Price-Whelan, Alexa / Nirody, Jasmine A / Dietrich, Lars E P

    PLoS biology

    2024  Volume 22, Issue 2, Page(s) e3002205

    Abstract: Cells must access resources to survive, and the anatomy of multicellular structures influences this access. In diverse multicellular eukaryotes, resources are provided by internal conduits that allow substances to travel more readily through tissue than ... ...

    Abstract Cells must access resources to survive, and the anatomy of multicellular structures influences this access. In diverse multicellular eukaryotes, resources are provided by internal conduits that allow substances to travel more readily through tissue than they would via diffusion. Microbes growing in multicellular structures, called biofilms, are also affected by differential access to resources and we hypothesized that this is influenced by the physical arrangement of the cells. In this study, we examined the microanatomy of biofilms formed by the pathogenic bacterium Pseudomonas aeruginosa and discovered that clonal cells form striations that are packed lengthwise across most of a mature biofilm's depth. We identified mutants, including those defective in pilus function and in O-antigen attachment, that show alterations to this lengthwise packing phenotype. Consistent with the notion that cellular arrangement affects access to resources within the biofilm, we found that while the wild type shows even distribution of tested substrates across depth, the mutants show accumulation of substrates at the biofilm boundaries. Furthermore, we found that altered cellular arrangement within biofilms affects the localization of metabolic activity, the survival of resident cells, and the susceptibility of subpopulations to antibiotic treatment. Our observations provide insight into cellular features that determine biofilm microanatomy, with consequences for physiological differentiation and drug sensitivity.
    MeSH term(s) Humans ; Anti-Bacterial Agents/pharmacology ; Pseudomonas aeruginosa/metabolism ; Biofilms ; Pseudomonas Infections/microbiology ; Fimbriae, Bacterial
    Chemical Substances Anti-Bacterial Agents
    Language English
    Publishing date 2024-02-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2126776-5
    ISSN 1545-7885 ; 1544-9173
    ISSN (online) 1545-7885
    ISSN 1544-9173
    DOI 10.1371/journal.pbio.3002205
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 6193: Show issues Location:
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  9. Article: The L-lactate dehydrogenases of

    Florek, Lindsey C / Lin, Xi / Lin, Yu-Cheng / Lin, Min-Han / Chakraborty, Arijit / Price-Whelan, Alexa / Tong, Liang / Rahme, Laurence / Dietrich, Lars E P

    bioRxiv : the preprint server for biology

    2024  

    Abstract: Pseudomonas ... ...

    Abstract Pseudomonas aeruginosa
    Language English
    Publishing date 2024-03-23
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2024.03.21.586142
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: The histidine kinase NahK regulates pyocyanin production through the PQS system.

    Mendoza, Alicia G / Guercio, Danielle / Smiley, Marina K / Sharma, Gaurav K / Withorn, Jason M / Hudson-Smith, Natalie V / Ndukwe, Chika / Dietrich, Lars E P / Boon, Elizabeth M

    Journal of bacteriology

    2024  Volume 206, Issue 1, Page(s) e0027623

    Abstract: Many bacterial histidine kinases work in two-component systems that combine into larger multi-kinase networks. NahK is one of the kinases in the GacS Multi-Kinase Network (MKN), which is the MKN that controls biofilm regulation in the opportunistic ... ...

    Abstract Many bacterial histidine kinases work in two-component systems that combine into larger multi-kinase networks. NahK is one of the kinases in the GacS Multi-Kinase Network (MKN), which is the MKN that controls biofilm regulation in the opportunistic pathogen
    MeSH term(s) Pyocyanine ; Histidine Kinase/genetics ; Histidine Kinase/metabolism ; Biofilms ; Quorum Sensing ; Virulence Factors/metabolism ; Bacteria/metabolism ; Pseudomonas aeruginosa/metabolism ; Bacterial Proteins/metabolism ; Anti-Bacterial Agents/pharmacology
    Chemical Substances Pyocyanine (9OQM399341) ; Histidine Kinase (EC 2.7.13.1) ; Virulence Factors ; Bacterial Proteins ; Anti-Bacterial Agents
    Language English
    Publishing date 2024-01-03
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2968-3
    ISSN 1098-5530 ; 0021-9193
    ISSN (online) 1098-5530
    ISSN 0021-9193
    DOI 10.1128/jb.00276-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.50: Show issues Location:
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