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  1. Article ; Online: Clustering of vomeronasal receptor genes is required for transcriptional stability but not for choice.

    Dietschi, Quentin / Tuberosa, Joël / Fodoulian, Leon / Boillat, Madlaina / Kan, Chenda / Codourey, Julien / Pauli, Véronique / Feinstein, Paul / Carleton, Alan / Rodriguez, Ivan

    Science advances

    2022  Volume 8, Issue 46, Page(s) eabn7450

    Abstract: Rodents perceive pheromones via vomeronasal receptors encoded by highly evolutionarily dynamic Vr and Fpr gene superfamilies. We report here that high numbers of V1r pseudogenes are scattered in mammalian genomes, contrasting with the clustered ... ...

    Abstract Rodents perceive pheromones via vomeronasal receptors encoded by highly evolutionarily dynamic Vr and Fpr gene superfamilies. We report here that high numbers of V1r pseudogenes are scattered in mammalian genomes, contrasting with the clustered organization of functional V1r and Fpr genes. We also found that V1r pseudogenes are more likely to be expressed when located in a functional V1r gene cluster than when isolated. To explore the potential regulatory role played by the association of functional vomeronasal receptor genes with their clusters, we dissociated the mouse
    Language English
    Publishing date 2022-11-16
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2810933-8
    ISSN 2375-2548 ; 2375-2548
    ISSN (online) 2375-2548
    ISSN 2375-2548
    DOI 10.1126/sciadv.abn7450
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Control of the Two-photon Visual Process in ex vivo Retinas and in Living Mice.

    Gaulier, Geoffrey / Dietschi, Quentin / Djorovic, Aleksa / La Volpe, Luca / Rodrigues, Tania / Bonacina, Luigi / Rodriguez, Ivan / Wolf, Jean-Pierre

    Chimia

    2022  Volume 76, Issue 6, Page(s) 570–574

    Abstract: Palcewska et al. first demonstrated near infrared (NIR) visual response in human volunteers upon two-photon absorption (TPA), in a seminal work of 2014, and assessed the process in terms of wavelength- and power-dependence on murine ex-vivo retinas. In ... ...

    Abstract Palcewska et al. first demonstrated near infrared (NIR) visual response in human volunteers upon two-photon absorption (TPA), in a seminal work of 2014, and assessed the process in terms of wavelength- and power-dependence on murine ex-vivo retinas. In the present study, ex-vivo electroretinography (ERG) is further developed to perform a complete characterization of the effect of NIR pulse duration, energy, and focal spot size on the response. The same set of measurements is successively tested on living mice. We discuss how the nonlinear intensity dependence of the photon absorption process is transferred to the amplitude of the visual response acquired by ERG. Finally, we show that the manipulation of the spectral phase of NIR pulses can be translated to predictable change in the two-photon induced response under physiological excitation conditions.
    Language English
    Publishing date 2022-06-29
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1516-7
    ISSN 0009-4293
    ISSN 0009-4293
    DOI 10.2533/chimia.2022.570
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

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  3. Article ; Online: Ultrafast pulse shaping modulates perceived visual brightness in living animals.

    Gaulier, Geoffrey / Dietschi, Quentin / Bhattacharyya, Swarnendu / Schmidt, Cédric / Montagnese, Matteo / Chauvet, Adrien / Hermelin, Sylvain / Chiodini, Florence / Bonacina, Luigi / Herrera, Pedro L / Rothlisberger, Ursula / Rodriguez, Ivan / Wolf, Jean-Pierre

    Science advances

    2021  Volume 7, Issue 18

    Abstract: Vision is usually assumed to be sensitive to the light intensity and spectrum but not to its spectral phase. However, experiments performed on retinal proteins in solution showed that the first step of vision consists in an ultrafast photoisomerization ... ...

    Abstract Vision is usually assumed to be sensitive to the light intensity and spectrum but not to its spectral phase. However, experiments performed on retinal proteins in solution showed that the first step of vision consists in an ultrafast photoisomerization that can be coherently controlled by shaping the phase of femtosecond laser pulses, especially in the multiphoton interaction regime. The link between these experiments in solution and the biological process allowing vision was not demonstrated. Here, we measure the electric signals fired from the retina of living mice upon femtosecond multipulse and single-pulse light stimulation. Our results show that the electrophysiological signaling is sensitive to the manipulation of the light excitation on a femtosecond time scale. The mechanism relies on multiple interactions with the light pulses close to the conical intersection, like pump-dump (photoisomerization interruption) and pump-repump (reverse isomerization) processes. This interpretation is supported both experimentally and by dynamics simulations.
    MeSH term(s) Animals ; Light ; Mice
    Language English
    Publishing date 2021-04-28
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2810933-8
    ISSN 2375-2548 ; 2375-2548
    ISSN (online) 2375-2548
    ISSN 2375-2548
    DOI 10.1126/sciadv.abe1911
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Evolution of immune chemoreceptors into sensors of the outside world.

    Dietschi, Quentin / Tuberosa, Joël / Rösingh, Lone / Loichot, Gregory / Ruedi, Manuel / Carleton, Alan / Rodriguez, Ivan

    Proceedings of the National Academy of Sciences of the United States of America

    2017  Volume 114, Issue 28, Page(s) 7397–7402

    Abstract: Changes in gene expression patterns represent an essential source of evolutionary innovation. A striking case of neofunctionalization is the acquisition of neuronal specificity by immune formyl peptide receptors (Fprs). In mammals, Fprs are expressed by ... ...

    Abstract Changes in gene expression patterns represent an essential source of evolutionary innovation. A striking case of neofunctionalization is the acquisition of neuronal specificity by immune formyl peptide receptors (Fprs). In mammals, Fprs are expressed by immune cells, where they detect pathogenic and inflammatory chemical cues. In rodents, these receptors are also expressed by sensory neurons of the vomeronasal organ, an olfactory structure mediating innate avoidance behaviors. Here we show that two gene shuffling events led to two independent acquisitions of neuronal specificity by Fprs. The first event targeted the promoter of a V1R receptor gene. This was followed some 30 million years later by a second genomic accident targeting the promoter of a V2R gene. Finally, we show that expression of a vomeronasal Fpr can reverse back to the immune system under inflammatory conditions via the production of an intergenic transcript linking neuronal and immune Fpr genes. Thus, three hijackings of regulatory elements are sufficient to explain all aspects of the complex expression patterns acquired by a receptor family that switched from sensing pathogens inside the organism to sensing the outside world through the nose.
    Language English
    Publishing date 2017-07-11
    Publishing country United States
    Document type Journal Article
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    DOI 10.1073/pnas.1704009114
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1148: Show issues Location:
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    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
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  5. Article ; Online: Convergence of FPR-rs3-expressing neurons in the mouse accessory olfactory bulb.

    Dietschi, Quentin / Assens, Alexis / Challet, Ludivine / Carleton, Alan / Rodriguez, Ivan

    Molecular and cellular neurosciences

    2013  Volume 56, Page(s) 140–147

    Abstract: In the mouse, most members of the FPR receptor family are expressed by vomeronasal sensory neurons. The neural circuitry corresponding to this class of chemical sensors is unknown. Taking advantage of the presence of FPR-rs3 on both vomeronasal dendrites ...

    Abstract In the mouse, most members of the FPR receptor family are expressed by vomeronasal sensory neurons. The neural circuitry corresponding to this class of chemical sensors is unknown. Taking advantage of the presence of FPR-rs3 on both vomeronasal dendrites and axonal fibers, we visualized the distribution of sensory cells expressing this member of the FPR family, and their corresponding axonal projections in the olfactory bulb. We found a rostrocaudal gradient of receptor choice frequency in the vomeronasal sensory neuroepithelium, and observed a convergence of FPR-rs3 axons into multiple, linked and deeply located glomeruli. These were homogenously innervated, and spatially restricted to the basal portion of the rostral accessory olfactory bulb. This organization, reminiscent of the one that characterizes axonal projections of V1R-expressing neurons, supports a role played by these receptors in the perception of semiochemicals.
    MeSH term(s) Animals ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Olfactory Bulb/cytology ; Olfactory Bulb/metabolism ; Receptors, Formyl Peptide/genetics ; Receptors, Formyl Peptide/metabolism ; Receptors, Pheromone/genetics ; Receptors, Pheromone/metabolism ; Sensory Receptor Cells/classification ; Sensory Receptor Cells/metabolism ; Vomeronasal Organ/cytology ; Vomeronasal Organ/metabolism
    Chemical Substances Receptors, Formyl Peptide ; Receptors, Pheromone ; V1rb2 protein, mouse
    Language English
    Publishing date 2013-09
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1046640-x
    ISSN 1095-9327 ; 1044-7431
    ISSN (online) 1095-9327
    ISSN 1044-7431
    DOI 10.1016/j.mcn.2013.04.008
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3035: Show issues Location:
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  6. Article ; Online: Alteration of Nrp1 signaling at different stages of olfactory neuron maturation promotes glomerular shifts along distinct axes in the olfactory bulb.

    Assens, Alexis / Dal Col, Julien A / Njoku, Anthony / Dietschi, Quentin / Kan, Chenda / Feinstein, Paul / Carleton, Alan / Rodriguez, Ivan

    Development (Cambridge, England)

    2016  Volume 143, Issue 20, Page(s) 3817–3825

    Abstract: Building the topographic map in the mammalian olfactory bulb is explained by a model based on two axes along which sensory neurons are guided: one dorsoventral and one anteroposterior. This latter axis relies on specific expression levels of Nrp1. To ... ...

    Abstract Building the topographic map in the mammalian olfactory bulb is explained by a model based on two axes along which sensory neurons are guided: one dorsoventral and one anteroposterior. This latter axis relies on specific expression levels of Nrp1. To evaluate the role of this receptor in this process, we used an in vivo genetic approach to decrease or suppress Nrp1 in specific neuronal populations and at different time points during axonal targeting. We observed, in neurons that express the M71 or M72 odorant receptors, that Nrp1 inactivation leads to two distinct wiring alterations, depending on the time at which Nrp1 expression is altered: first, a surprising dorsal shift of the M71 and M72 glomeruli, which often fuse with their contralateral counterparts, and second the formation of anteriorized glomeruli. The two phenotypes are partly recapitulated in mice lacking the Nrp1 ligand Sema3A and in mice whose sensory neurons express an Nrp1 mutant unable to bind Sema3A. Using a mosaic conditional approach, we show that M71 axonal fibers can bypass the Nrp1 signals that define their target area, since they are hijacked and coalesce with Nrp1-deficient M71-expressing axons that target elsewhere. Together, these findings show drastically different axonal targeting outcomes dependent on the timing at which Nrp1/Sema3A signaling is altered.
    MeSH term(s) Animals ; Axons/metabolism ; Gene Expression Regulation, Developmental/genetics ; Gene Expression Regulation, Developmental/physiology ; Immunohistochemistry ; Mice ; Neuropilin-1/genetics ; Neuropilin-1/metabolism ; Olfactory Bulb/cytology ; Olfactory Bulb/embryology ; Olfactory Bulb/metabolism ; Olfactory Receptor Neurons/cytology ; Olfactory Receptor Neurons/metabolism ; Semaphorin-3A/genetics ; Semaphorin-3A/metabolism
    Chemical Substances Semaphorin-3A ; Neuropilin-1 (144713-63-3)
    Language English
    Publishing date 2016--15
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 90607-4
    ISSN 1477-9129 ; 0950-1991
    ISSN (online) 1477-9129
    ISSN 0950-1991
    DOI 10.1242/dev.138941
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ub I Zs.183: Show issues Location:
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