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  1. Article ; Online: UPLC-QTOF MS-Based Serum Metabolomic Profiling Analysis Reveals the Molecular Perturbations Underlying Uremic Pruritus.

    Wu, Qiong / Zhang, Huan / Ding, Jia-Rong / Hong, Zhan-Ying / Wu, Hao / Zhu, Zhen-Yu / Guo, Zhi-Yong / Chai, Yi-Feng

    BioMed research international

    2018  Volume 2018, Page(s) 4351674

    Abstract: As one of the most troublesome complications in patients with chronic renal disease, the etiology of uremic pruritus remains unknown, and the current therapeutic approaches are limited and unsatisfactory. To identify potential biomarkers for improving ... ...

    Abstract As one of the most troublesome complications in patients with chronic renal disease, the etiology of uremic pruritus remains unknown, and the current therapeutic approaches are limited and unsatisfactory. To identify potential biomarkers for improving diagnosis and treatment and obtain a better understanding of the pathogenesis of uremic pruritus, we compared serum metabolome profiles of severe uremic pruritus (HUP) patients with mild uremic pruritus (LUP) patients using ultraperformance liquid chromatography-quadruple time-of-flight mass spectrometry (UPLC-QTOF MS). Partial least squares discriminant analysis (PLS-DA) showed that the metabolic profiles of HUP patients are distinguishable from those of LUP patients. Combining multivariate with univariate analysis, 22 significantly different metabolites between HUP and LUP patients were identified. Nine of the 22 metabolites in combination were characterized by a maximum area-under-receiver operating characteristic curve (AUC = 0.899) with a sensitivity of 85.1% and a specificity of 83.0% distinguishing HUP and LUP. Our results indicate that serum metabolome profiling might serve as a promising approach for the diagnosis of uremic pruritus and that the identified biomarkers may improve the understanding of pathophysiology of this disorder. Because the 9 metabolites were phospholipids, uremic toxins, and steroids, further studies may reveal their possible role in the pathogenesis of uremic pruritus.
    MeSH term(s) Adult ; Biomarkers/blood ; Chromatography, High Pressure Liquid ; Female ; Humans ; Male ; Metabolome ; Metabolomics ; Middle Aged ; Pruritus/blood ; Pruritus/pathology ; Tandem Mass Spectrometry
    Chemical Substances Biomarkers
    Language English
    Publishing date 2018-01-10
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2698540-8
    ISSN 2314-6141 ; 2314-6133
    ISSN (online) 2314-6141
    ISSN 2314-6133
    DOI 10.1155/2018/4351674
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Fu-Fang-Jin-Qian-Cao herbal granules protect against the calcium oxalate-induced renal EMT by inhibiting the TGF-β/smad pathway.

    Liu, Wen-Rui / Lu, Hong-Tao / Zhao, Ting-Ting / Ding, Jia-Rong / Si, Ya-Chen / Chen, Wei / Hou, Jie-Bin / Gao, Song-Yan / Dong, Xin / Yu, Bing / Guo, Zhi-Yong / Lu, Jian-Rao

    Pharmaceutical biology

    2020  Volume 58, Issue 1, Page(s) 1115–1122

    Abstract: Context: Nephrolithiasis is a major public health problem worldwide and Fu-Fang-Jin-Qian-Cao granules (FFJQC) is a traditional Chinese herbal formula that is used to treat nephrolithiasis. The main component of nephrolithiasis is calcium oxalate (CaOx) ... ...

    Abstract Context: Nephrolithiasis is a major public health problem worldwide and Fu-Fang-Jin-Qian-Cao granules (FFJQC) is a traditional Chinese herbal formula that is used to treat nephrolithiasis. The main component of nephrolithiasis is calcium oxalate (CaOx) and the epithelial-mesenchymal transition (EMT) shown to play a crucial role in CaOx-induced kidney injury. However, the mechanism underlying the therapeutic effect of FFJQC on the CaOx-induced renal EMT is unknown.
    Objective: This study explores the therapeutic benefits and mechanism of FFJQC in oxalate-induced kidney injury.
    Materials and methods: 60 male C57BL/6 mice were used in this experiment and divided into 6 groups. A mouse kidney stone model was created by intraperitoneal injection of glyoxylate at a dose of 100 mg/kg for 6 days. The standardized FFJQC was used to treat mouse crystal kidney injury by gavage at 1.35 and 2.7 g/kg, respectively. Western blotting and immunostaining for E-cadherin, cytokeratin 18 (CK18), vimentin, smooth muscle α-actin (α-SMA) and transforming growth factor β (TGF-β)/Smad pathway were conducted on renal tissues.
    Results: Following CaOx-induced kidney injury, the levels of E-cadherin and CK18 in kidney decreased, while vimentin and α-SMA levels increased. The FFJQC treatment increased the levels of E-cadherin and CK18 and decreased vimentin and α-SMA levels in varying degrees. What's more, the FFJQC reduced the expression of CaOx-induced fibrosis marker collagen II.
    Conclusion: FFJQC alleviated the CaOx-induced renal EMT and fibrosis by regulating TGF-β/smad pathway. Therefore, the FFJQC is an important traditional Chinese medicine for the treatment of CaOx-induced renal injury and fibrosis.
    MeSH term(s) Animals ; Cadherins/metabolism ; Calcium Oxalate/toxicity ; Disease Models, Animal ; Dose-Response Relationship, Drug ; Drugs, Chinese Herbal/administration & dosage ; Drugs, Chinese Herbal/pharmacology ; Epithelial-Mesenchymal Transition/drug effects ; Kidney Calculi/prevention & control ; Male ; Mice ; Mice, Inbred C57BL ; Nephrolithiasis/prevention & control ; Signal Transduction/drug effects ; Smad Proteins/metabolism ; Transforming Growth Factor beta/metabolism
    Chemical Substances Cadherins ; Drugs, Chinese Herbal ; Smad Proteins ; Transforming Growth Factor beta ; Calcium Oxalate (2612HC57YE)
    Language English
    Publishing date 2020-11-16
    Publishing country England
    Document type Comparative Study ; Journal Article
    ZDB-ID 1440131-9
    ISSN 1744-5116 ; 1388-0209
    ISSN (online) 1744-5116
    ISSN 1388-0209
    DOI 10.1080/13880209.2020.1844241
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Fu-Fang-Jin-Qian-Cao herbal granules protect against the calcium oxalate-induced renal EMT by inhibiting the TGF-β/smad pathway

    Liu, Wen-Rui / Lu, Hong-Tao / Zhao, Ting-Ting / Ding, Jia-Rong / Si, Ya-Chen / Chen, Wei / Hou, Jie-Bin / Gao, Song-Yan / Dong, Xin / Yu, Bing / Guo, Zhi-Yong / Lu, Jian-Rao

    Pharmaceutical biology. 2020 Jan. 1, v. 58, no. 1

    2020  

    Abstract: Nephrolithiasis is a major public health problem worldwide and Fu-Fang-Jin-Qian-Cao granules (FFJQC) is a traditional Chinese herbal formula that is used to treat nephrolithiasis. The main component of nephrolithiasis is calcium oxalate (CaOx) and the ... ...

    Abstract Nephrolithiasis is a major public health problem worldwide and Fu-Fang-Jin-Qian-Cao granules (FFJQC) is a traditional Chinese herbal formula that is used to treat nephrolithiasis. The main component of nephrolithiasis is calcium oxalate (CaOx) and the epithelial-mesenchymal transition (EMT) shown to play a crucial role in CaOx-induced kidney injury. However, the mechanism underlying the therapeutic effect of FFJQC on the CaOx-induced renal EMT is unknown. This study explores the therapeutic benefits and mechanism of FFJQC in oxalate-induced kidney injury. 60 male C57BL/6 mice were used in this experiment and divided into 6 groups. A mouse kidney stone model was created by intraperitoneal injection of glyoxylate at a dose of 100 mg/kg for 6 days. The standardized FFJQC was used to treat mouse crystal kidney injury by gavage at 1.35 and 2.7 g/kg, respectively. Western blotting and immunostaining for E-cadherin, cytokeratin 18 (CK18), vimentin, smooth muscle α-actin (α-SMA) and transforming growth factor β (TGF-β)/Smad pathway were conducted on renal tissues. Following CaOx-induced kidney injury, the levels of E-cadherin and CK18 in kidney decreased, while vimentin and α-SMA levels increased. The FFJQC treatment increased the levels of E-cadherin and CK18 and decreased vimentin and α-SMA levels in varying degrees. What’s more, the FFJQC reduced the expression of CaOx-induced fibrosis marker collagen II. FFJQC alleviated the CaOx-induced renal EMT and fibrosis by regulating TGF-β/smad pathway. Therefore, the FFJQC is an important traditional Chinese medicine for the treatment of CaOx-induced renal injury and fibrosis.
    Keywords Oriental traditional medicine ; cadherins ; calcium ; calcium oxalate ; collagen ; fibrosis ; intraperitoneal injection ; kidneys ; males ; mice ; models ; public health ; renal calculi ; smooth muscle ; therapeutics ; vimentin
    Language English
    Dates of publication 2020-0101
    Size p. 1124-1131.
    Publishing place Taylor & Francis
    Document type Article
    ZDB-ID 1440131-9
    ISSN 1744-5116 ; 1388-0209
    ISSN (online) 1744-5116
    ISSN 1388-0209
    DOI 10.1080/13880209.2020.1844241
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: Metabolomic analysis reveals a protective effect of Fu-Fang-Jin-Qian-Chao herbal granules on oxalate-induced kidney injury.

    Chen, Wei / Liu, Wen-Rui / Hou, Jie-Bin / Ding, Jia-Rong / Peng, Zhong-Jiang / Gao, Song-Yan / Dong, Xin / Ma, Jun-Hua / Lin, Qi-Shan / Lu, Jian-Rao / Guo, Zhi-Yong

    Bioscience reports

    2019  Volume 39, Issue 2

    Abstract: Nephrolithiasis is one of the world's major public health burdens with a high incidence and a risk of persistent renal dysfunction. Fu-Fang-Jin-Qian-Chao granules (FFJQC), a traditional Chinese herb formula, is commonly used in treatment of ... ...

    Abstract Nephrolithiasis is one of the world's major public health burdens with a high incidence and a risk of persistent renal dysfunction. Fu-Fang-Jin-Qian-Chao granules (FFJQC), a traditional Chinese herb formula, is commonly used in treatment of nephrolithiasis. However, the therapeutic mechanism of FFJQC on kidney stone has still been a mystery. The objective of the present study is to explore the therapeutic mechanism of FFJQC on kidney injury and identify unique metabolomics patterns using a mouse model of kidney stone induced by a calcium oxalate (CaOx) deposition. Von Kossa staining and immuno-histopathological staining of osteopontin (OPN), cluster of differentiation 44 (CD44) and calbindin-D28k were conducted on renal sections. Biochemical analysis was performed on serum, urine, and kidney tissues. A metabolomics approach based on ultra-HPLC coupled with quadrupole-TOF-MS (UHPLC-Q-TOF/MS) was used for serum metabolic profiling. The immunohistopathological and biochemical analysis showed the therapeutic benefits of FFJQC. The expression levels of OPN and CD44 were decreased while calbindin-D28k increased after the CaOx injured mice were treated with FFJQC. In addition, total of 81 serum metabolites were identified to be associated with protective effects of FFJQC on CaOx crystal injured mice. Most of these metabolites were involved in purine, amino acid, membrane lipid and energy metabolism. Potential metabolite biomarkers were found for CaOx crystal-induced renal damage. Potential metabolite biomarkers of CaOx crystal-induced renal damage were found. FFJQC shows therapeutic benefits on CaOx crystal injured mice via regulation of multiple metabolic pathways including amino acids, purine, pyrimidine, glycerolipid, arachidonic acid (AA), sphingolipid, glycerophospholipid, and fatty acid.
    MeSH term(s) Animals ; Calcium Oxalate/adverse effects ; Disease Models, Animal ; Drugs, Chinese Herbal/therapeutic use ; Kidney/drug effects ; Kidney/metabolism ; Kidney/pathology ; Kidney Calculi/drug therapy ; Kidney Calculi/etiology ; Kidney Calculi/metabolism ; Kidney Calculi/pathology ; Male ; Metabolome/drug effects ; Metabolomics ; Mice, Inbred C57BL ; Protective Agents/therapeutic use
    Chemical Substances Drugs, Chinese Herbal ; Protective Agents ; Calcium Oxalate (2612HC57YE)
    Language English
    Publishing date 2019-02-22
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 764946-0
    ISSN 1573-4935 ; 0144-8463
    ISSN (online) 1573-4935
    ISSN 0144-8463
    DOI 10.1042/BSR20181833
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 1676: Show issues Location:
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  5. Article ; Online: Gastrointestinal symptoms: a comparison between patients undergoing peritoneal dialysis and hemodialysis.

    Dong, Rui / Guo, Zhi-Yong / Ding, Jia-Rong / Zhou, Yang-Yang / Wu, Hao

    World journal of gastroenterology

    2013  Volume 20, Issue 32, Page(s) 11370–11375

    Abstract: Aim: To compare the prevalence and diversity of gastrointestinal (GI) symptoms in patients undergoing peritoneal dialysis (PD) and hemodialysis (HD).: Methods: Two hundred and ninety-four end-stage renal disease patients participated in the study, ... ...

    Abstract Aim: To compare the prevalence and diversity of gastrointestinal (GI) symptoms in patients undergoing peritoneal dialysis (PD) and hemodialysis (HD).
    Methods: Two hundred and ninety-four end-stage renal disease patients participated in the study, including 182 HD and 112 PD patients. Dimension scores were calculated from a modified gastrointestinal symptom rating scale (GSRS) 18-item questionnaire, including items concerning eating dysfunction, and were used for measuring GI symptoms. Information on patient age, condition contributing to end-stage renal disease and the most recent dialysis adequacy assessment (serum Kt/V urea value) was obtained from the follow-up database and by interviewing patients and/or reviewing the medical records. Differences between the HD and PD groups were evaluated using Student's t, Pearson's χ(2) or Fisher's exact tests.
    Results: The overall prevalence of GI symptoms, defined by a GSRS > 1, in end-stage renal disease patients was 70.7% (208/294), which differed between HD and PD patients (76.4% vs 61.6%, P < 0.01). HD patients had a higher prevalence of constipation, abdominal pain and diarrhea compared to PD patients (36.3% vs 17.9%, 32.4% vs 5.4%, 17.6% vs 4.5%, respectively, P < 0.05). PD patients had a higher prevalence of reflux compared to HD patients (32.1% vs 24.2%, P < 0.05). Additionally, reflux and eating dysfunction were more severe in PD patients (GSRS: 1.71 ± 1.15 vs 1.30 ± 0.67, 1.57 ± 0.84 vs 1.39 ± 0.61, respectively, P < 0.05), whereas HD patients had greater abdominal pain, diarrhea and constipation (GSRS: 1.22 ± 0.39 vs 1.04 ± 0.19, 1.19 ± 0.53 vs 1.07 ± 0.35, 1.51 ± 0.83 vs 1.23 ± 0.58, respectively, P < 0.05). Finally, 14.8% (27/182) of HD patients presented with more than three GI symptoms, compared to 7.2% (8/112) of PD patients (P < 0.01).
    Conclusion: HD and PD patients differ in prevalence, severity and diversity of GI symptoms.
    MeSH term(s) Adult ; Aged ; Chi-Square Distribution ; China/epidemiology ; Female ; Gastrointestinal Diseases/diagnosis ; Gastrointestinal Diseases/epidemiology ; Humans ; Kidney Failure, Chronic/diagnosis ; Kidney Failure, Chronic/epidemiology ; Kidney Failure, Chronic/therapy ; Male ; Middle Aged ; Peritoneal Dialysis/adverse effects ; Prevalence ; Renal Dialysis/adverse effects ; Retrospective Studies ; Risk Factors ; Severity of Illness Index ; Surveys and Questionnaires ; Treatment Outcome
    Language English
    Publishing date 2013-03-14
    Publishing country United States
    Document type Comparative Study ; Journal Article
    ZDB-ID 2185929-2
    ISSN 2219-2840 ; 1007-9327
    ISSN (online) 2219-2840
    ISSN 1007-9327
    DOI 10.3748/wjg.v20.i32.11370
    Database MEDical Literature Analysis and Retrieval System OnLINE

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