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Article: The insecticidal activity of methyl benzoate against Tribolium castaneum by transcriptomic analysis and in-silico simulation

Wang, Lei / Ding, Sai-sai / Zhang, Ning-jing / Lu, Yujie / Geng, Xueqing / Zhao, Zongpei

Journal of stored products research. 2022 May, v. 97

2022

Abstract: Plant-derived methyl benzoate (MB) is a scent and aroma released by many flowers and fruits. Recently, MB has emerged as an alternative pesticide to control insect pests. However, the molecular mechanisms of insects' responses to MB remain to be ... ...

Abstract	Plant-derived methyl benzoate (MB) is a scent and aroma released by many flowers and fruits. Recently, MB has emerged as an alternative pesticide to control insect pests. However, the molecular mechanisms of insects' responses to MB remain to be investigated. MB exhibited a dose-dependent toxicity to all the developmental stages of Tribolium castaneum (Herbst). Based on the PoloPlus analysis of the lethal median concentration (LC₅₀) of contact/fumigant toxicity for eggs, larvae, pupae, and adults, our study indicated that larvae appeared to be most resilient to MB, and eggs were the most susceptible life stage. Next, we used RNA-seq method to analyze MB-response genes in T. castaneum larvae. Total of 1061 differentially expressed genes (DEGs) were identified, and analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment. The transcriptional expressions of identified genes were validated by real-time quantitative polymerase chain reaction (RT-qPCR). RNA-seq results indicated that genes that involved chitin biosynthesis, energy metabolism, nervous action, and metabolic detoxification were shown to be differentially expressed. Notably, gene expressions of cathepsin (B and L type), and total gut proteolytic activity were inhibited in MB-contacted/fumigated larvae, suggesting that MB exposure led to the suppression of digestive activity. And molecular dynamics results indicated that OBP99b could stably bind with MB. This research will enrich our knowledge of the molecular mechanisms of beetle's response to MB, and facilitate us to develop an efficient strategy to control insect pests.
Keywords	Tribolium castaneum ; biosynthesis ; cathepsins ; chitin ; computer simulation ; digestive system ; dose response ; energy metabolism ; fumigants ; gene expression regulation ; gene ontology ; genes ; insecticidal properties ; metabolic detoxification ; molecular dynamics ; odors ; pesticides ; proteolysis ; quantitative polymerase chain reaction ; research ; sequence analysis ; toxicity ; transcription (genetics) ; transcriptomics
Language	English
Dates of publication	2022-05
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	410225-3
ISSN	0022-474X
ISSN	0022-474X
DOI	10.1016/j.jspr.2022.101972
Database	NAL-Catalogue (AGRICOLA)

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Article: Induced hepatic stem cells maintain self-renewal through the high expression of Myc coregulated by TET1 and CTCF.

Wang, Chen / Yu, Xinlu / Ding, Sai / Liu, Yang / Zhang, Hongxia / Fu, Jingbo / Yu, Bing / Zhu, Haiying

Cell & bioscience

2022 Volume 12, Issue 1, Page(s) 143

Abstract: Background: Induced hepatic stem cells (iHepSCs) with the capacities of self-renewal and bidifferentiation into hepatocytes and cholangiocytes were generated from mouse embryonic fibroblasts (MEFs) by lineage reprogramming in our previous research. ... ...

Abstract	Background: Induced hepatic stem cells (iHepSCs) with the capacities of self-renewal and bidifferentiation into hepatocytes and cholangiocytes were generated from mouse embryonic fibroblasts (MEFs) by lineage reprogramming in our previous research. However, the mechanism of iHepSC self-renewal has not been elucidated. Active demethylation regulated by Tet1 plays an important role in the self-renewal of stem cells, including pluripotent stem cells and adult stem cells. Here, we investigated the role and mechanism of Tet1-regulated demethylation in the self-renewal of iHepSCs. Methods: The methylation levels and the expression of Tet1 in iHepSCs and MEFs were analyzed by immunofluorescent staining, quantitative reverse transcription PCR and western blotting. Then, the effects of Tet1 knockdown on the proliferation and self-renewal of iHepSCs were analyzed by CCK8, colony formation, and sphere formation assays. The mechanism by which Tet1 regulates the self-renewal of iHepSCs was investigated by chromatin immunoprecipitation, bisulfite sequence PCR, and methylation-sensitive restriction endonuclease-PCR. Results: The high level of 5hmC and the low level of 5mC in iHepSCs were accompanied by high expression of Tet1. After Tet1 expression was knocked down by shRNA in iHepSCs, the proliferation and self-renewal capacities were inhibited, and the expression of Myc was also decreased. The higher expression level of Myc in iHepSCs maintained its self-renewal and was regulated by Tet1, which directly binds to CBS-1 and site A regions of the Myc promoter and demethylates the CpG cytosine. In addition, CTCF also binds to the CBS-1 and site A regions of the Myc promoter and regulates Myc expression along with TET1. Conclusion: The self-renewal of iHepSCs was maintained by the higher expression of Myc, which was coregulated by TET1 and CTCF. This study may provide new insights into the self-renewal of stem cells, which can promote the research and application of 'reprogrammed' stem cells.
Language	English
Publishing date	2022-09-02
Publishing country	England
Document type	Journal Article
ZDB-ID	2593367-X
ISSN	2045-3701
ISSN	2045-3701
DOI	10.1186/s13578-022-00883-7
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Immobilization of the extracellular recombinant Lucky9 xylanase from Bacillus subtilis enhances activity at high temperature and pH

Ding, Sai‐sai / Zhu, Jin‐peng / Wang, Yang / Wu, Bin / Zhao, Zongpei

FEBS Open Bio. 2020 Dec., v. 10, no. 12

2020

Abstract: In the paper industry, chlorine is often used to treat the pulp for bleaching. After pulping, a large amount of xylan is present in the fiber. Xylanase can be used to degrade xylan in an eco‐friendly process called biobleaching, which can help minimize ... ...

Abstract	In the paper industry, chlorine is often used to treat the pulp for bleaching. After pulping, a large amount of xylan is present in the fiber. Xylanase can be used to degrade xylan in an eco‐friendly process called biobleaching, which can help minimize the usage of chlorine in the delignification process. However, a bottleneck in the adoption of biobleaching is the cost of xylanase and the requirement that xylanase be active and stable at extreme conditions. Here, we investigated whether using sodium alginate beads to immobilize an extracellular xylanase from Bacillus subtilis (Lucky9) can reduce the potential cost of enzyme usage. The optimal pH and the activity of the immobilized enzyme were increased at optimal temperature compared with the free enzyme. In addition, immobilized xylanase was shown to be more stable than free xylanase. The results of this study suggest that the immobilized xylanase has potential applications in the biobleaching industry.
Keywords	Bacillus subtilis ; biobleaching ; chlorine ; delignification ; immobilized enzymes ; pH ; pulp ; pulp and paper industry ; sodium alginate ; temperature ; xylan ; xylanases
Language	English
Dates of publication	2020-12
Size	p. 2733-2739.
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	2651702-4
ISSN	2211-5463
ISSN	2211-5463
DOI	10.1002/2211-5463.13010
Database	NAL-Catalogue (AGRICOLA)

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Article: Unemployment and the rising number of nonworkers in urban China

Gustafsson, Björn / Ding, Sai

Rising inequality in China : challenges to a harmonious society , p. 289-331

causes and distributional consequences

2015 , Page(s) 289–331

Author's details	Björn Gustafsson and Din Sai
Keywords	Städtischer Arbeitsmarkt ; Arbeitslosigkeit ; Einkommensverteilung ; China
Language	English
Size	graph. Darst.
Publisher	Cambridge Univ Press
Publishing place	New York, NY
Document type	Article
ISBN	1-107-52926-3 ; 978-1-107-52926-7
Database	ECONomics Information System

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Article ; Online: Immobilization of the extracellular recombinant Lucky9 xylanase from Bacillus subtilis enhances activity at high temperature and pH.

Ding, Sai-Sai / Zhu, Jin-Peng / Wang, Yang / Wu, Bin / Zhao, Zongpei

FEBS open bio

2020 Volume 10, Issue 12, Page(s) 2733–2739

Abstract: In the paper industry, chlorine is often used to treat the pulp for bleaching. After pulping, a large amount of xylan is present in the fiber. Xylanase can be used to degrade xylan in an eco-friendly process called biobleaching, which can help minimize ... ...

Abstract	In the paper industry, chlorine is often used to treat the pulp for bleaching. After pulping, a large amount of xylan is present in the fiber. Xylanase can be used to degrade xylan in an eco-friendly process called biobleaching, which can help minimize the usage of chlorine in the delignification process. However, a bottleneck in the adoption of biobleaching is the cost of xylanase and the requirement that xylanase be active and stable at extreme conditions. Here, we investigated whether using sodium alginate beads to immobilize an extracellular xylanase from Bacillus subtilis (Lucky9) can reduce the potential cost of enzyme usage. The optimal pH and the activity of the immobilized enzyme were increased at optimal temperature compared with the free enzyme. In addition, immobilized xylanase was shown to be more stable than free xylanase. The results of this study suggest that the immobilized xylanase has potential applications in the biobleaching industry.
MeSH term(s)	Bacillus subtilis/enzymology ; Endo-1,4-beta Xylanases/metabolism ; Enzymes, Immobilized/metabolism ; Hydrogen-Ion Concentration ; Temperature ; Xylans/metabolism
Chemical Substances	Enzymes, Immobilized ; Xylans ; Endo-1,4-beta Xylanases (EC 3.2.1.8)
Language	English
Publishing date	2020-11-09
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2651702-4
ISSN	2211-5463 ; 2211-5463
ISSN (online)	2211-5463
ISSN	2211-5463
DOI	10.1002/2211-5463.13010
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Does affirmative action in Chinese college admissions lead to mismatch? Educational quality and the relative returns to a baccalaureate degree for minorities in China.

Ding, Sai / Myers, Samuel L / Price, Gregory N

International journal of anthropology and ethnology

2017 Volume 1

Abstract: This paper provides a unique illustration of the benefits of affirmative action in higher educaiton. It focuses on China in the early ... ...

Abstract	This paper provides a unique illustration of the benefits of affirmative action in higher educaiton. It focuses on China in the early 21
Language	English
Publishing date	2017-12-08
Publishing country	China
Document type	Journal Article
ZDB-ID	2918110-0
ISSN	2366-1003 ; 2366-1003
ISSN (online)	2366-1003
ISSN	2366-1003
DOI	10.1186/s41257-017-0006-7
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Deep Learning Model Based on Dual-Modal Ultrasound and Molecular Data for Predicting Response to Neoadjuvant Chemotherapy in Breast Cancer.

Huang, Jia-Xin / Shi, Jun / Ding, Sai-Sai / Zhang, Hui-Li / Wang, Xue-Yan / Lin, Shi-Yang / Xu, Yan-Fen / Wei, Ming-Jie / Liu, Long-Zhong / Pei, Xiao-Qing

Academic radiology

2023 Volume 30 Suppl 2, Page(s) S50–S61

Abstract: Rationale and objectives: To carry out radiomics analysis/deep convolutional neural network (CNN) based on B-mode ultrasound (BUS) and shear wave elastography (SWE) to predict response to neoadjuvant chemotherapy (NAC) in breast cancer patients.: ... ...

Abstract	Rationale and objectives: To carry out radiomics analysis/deep convolutional neural network (CNN) based on B-mode ultrasound (BUS) and shear wave elastography (SWE) to predict response to neoadjuvant chemotherapy (NAC) in breast cancer patients. Materials and methods: In this prospective study, 255 breast cancer patients who received NAC between September 2016 and December 2021 were included. Radiomics models were designed using a support vector machine classifier based on US images obtained before treatment, including BUS and SWE. And CNN models also were developed using ResNet architecture. The final predictive model was developed by combining the dual-modal US and independently associated clinicopathologic characteristics. The predictive performances of the models were assessed with five-fold cross-validation. Results: Pretreatment SWE performed better than BUS in predicting the response to NAC for breast cancer for both the CNN and radiomics models (P < 0.001). The predictive results of the CNN models were significantly better than the radiomics models, with AUCs of 0.72 versus 0.69 for BUS and 0.80 versus 0.77 for SWE, respectively (P = 0.003). The CNN model based on the dual-modal US and molecular data exhibited outstanding performance in predicting NAC response, with an accuracy of 83.60% ± 2.63%, a sensitivity of 87.76% ± 6.44%, and a specificity of 77.45% ± 4.38%. Conclusion: The pretreatment CNN model based on the dual-modal US and molecular data achieved excellent performance for predicting the response to chemotherapy in breast cancer. Therefore, this model has the potential to serve as a non-invasive objective biomarker to predict NAC response and aid clinicians with individual treatments.
MeSH term(s)	Humans ; Female ; Breast Neoplasms/diagnostic imaging ; Breast Neoplasms/drug therapy ; Breast Neoplasms/pathology ; Deep Learning ; Neoadjuvant Therapy ; Prospective Studies ; Ultrasonography/methods ; Retrospective Studies
Language	English
Publishing date	2023-06-01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1355509-1
ISSN	1878-4046 ; 1076-6332
ISSN (online)	1878-4046
ISSN	1076-6332
DOI	10.1016/j.acra.2023.03.036
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Localization and promotion of recombinant human bone morphogenetic protein-2 bioactivity on extracellular matrix mimetic chondroitin sulfate-functionalized calcium phosphate cement scaffolds.

Huang, Baolin / Wu, Zihan / Ding, Sai / Yuan, Yuan / Liu, Changsheng

Acta biomaterialia

2018 Volume 71, Page(s) 184–199

Abstract: Localization of recombinant human bone morphogenetic protein-2 (rhBMP-2) with continuous and effective osteogenic stimulation is still a great challenge in the field of bone regeneration. To achieve this aim, rhBMP-2 was tethered on chondroitin sulfate ( ... ...

Abstract	Localization of recombinant human bone morphogenetic protein-2 (rhBMP-2) with continuous and effective osteogenic stimulation is still a great challenge in the field of bone regeneration. To achieve this aim, rhBMP-2 was tethered on chondroitin sulfate (CS)-functionalized calcium phosphate cement (CPC) scaffolds through specific noncovalent interactions. CS, one of the core glycosaminoglycans, was covalently conjugated onto CPC scaffolds with the assistance of polydopamine (PDA) and further immobilized rhBMP-2 in a biomimetic form. The CPC-PDA-CS scaffolds not only controlled the release kinetics and presentation state of rhBMP-2 but also effectively increased the expression levels of bone morphogenetic protein receptors (BMPRs) and enhanced the recognitions of the remaining rhBMP-2 to BMPRs. Strikingly, the rhBMP-2-loaded CPC-PDA-CS significantly promoted the cellular surface translocation of BMPRs (especially BMPR-IA). In vivo studies demonstrated that, compared with the rhBMP-2 upon CPC and CPC-PDA, the rhBMP-2 upon CPC-PDA-CS exhibited sustained release and induced high quality and more ectopic bone formation. Collectively, these results suggest that rhBMP-2 can be localized within CS-functionalized CPC scaffolds and exert continuous, long-term, and effective osteogenic stimulation. Thus, this work could provide new avenues in mimicking bone extracellular matrix microenvironment and localizing growth factor activity for enhanced bone regeneration. Statement of significance: A bioinspired chondroitin sulfate (CS)-functionalized calcium phosphate cement (CPC) platform was developed to tether recombinant human bone morphogenetic protein-2 (rhBMP-2), which could exhibit continuous, long-term, and effective osteogenic stimulation in bone tissue engineering. Compared with rhBMP-2-loaded CPC, the rhBMP-2-loaded CPC-polydopamine-CS scaffolds induced higher expression of bone morphogenetic protein receptors (BMPRs), greater cellular surface translocation of bone morphogenetic protein receptor-IA, higher binding affinity of BMPRs/rhBMP-2, and thus higher activation of the drosophila gene mothers against decapentaplegic protein-1/5/8 (Smad1/5/8) and extracellular-regulated protein kinases-1/2 (ERK1/2) signaling. This work can provide new guidelines for the design of BMP-2-based bioactive materials for bone regeneration.
MeSH term(s)	Animals ; Bone Cements/chemistry ; Bone Morphogenetic Protein 2/chemistry ; Bone Morphogenetic Protein 2/pharmacokinetics ; Calcium Phosphates/chemistry ; Chondroitin Sulfates/chemistry ; Delayed-Action Preparations/chemistry ; Delayed-Action Preparations/pharmacokinetics ; Extracellular Matrix/chemistry ; Humans ; Male ; Rats ; Rats, Sprague-Dawley ; Recombinant Proteins/chemistry ; Recombinant Proteins/pharmacokinetics
Chemical Substances	BMP2 protein, human ; Bone Cements ; Bone Morphogenetic Protein 2 ; Calcium Phosphates ; Delayed-Action Preparations ; Recombinant Proteins ; Chondroitin Sulfates (9007-28-7) ; calcium phosphate (97Z1WI3NDX)
Language	English
Publishing date	2018-01-31
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2173841-5
ISSN	1878-7568 ; 1742-7061
ISSN (online)	1878-7568
ISSN	1742-7061
DOI	10.1016/j.actbio.2018.01.004
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Book ; Online: Why is there no income gap between the Hui Muslim minority and the Han majority in rural Ningxia, China?

Gustafsson, Björn Anders / Ding, Sai

(Discussion paper series / Forschungsinstitut zur Zukunft der Arbeit ; 7970)

2014

Abstract: Using a household sample survey for 2006 we show that the Hui population in the rural part of Ningxia autonomous region of China is disadvantaged compared to the Han majority as regards length of education and household per capita wealth. Yet there is no ...

Author's details	Björn Gustafsson; Ding Sai
Series title	Discussion paper series / Forschungsinstitut zur Zukunft der Arbeit ; 7970
Abstract	Using a household sample survey for 2006 we show that the Hui population in the rural part of Ningxia autonomous region of China is disadvantaged compared to the Han majority as regards length of education and household per capita wealth. Yet there is no gap in average disposable income between the two ethnic groups and poverty rates are very similar. This paradox is due to members of Hui households earning more income outside the farm than members of Han households. Particularly young male Hui living in poor villages have a remarkably high likelihood of migrating, thereby bringing home income to their households.
Keywords	China ; ethnic minorities ; Hui ethnicity ; income ; poverty ; migration
Language	English
Size	Online-Ressource (38 S.), graph. Darst.
Publisher	IZA
Publishing place	Bonn
Document type	Book ; Online
Database	ECONomics Information System

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Article: Biofilm-based fermentation: a novel immobilisation strategy for Saccharomyces cerevisiae cell cycle progression during ethanol production

Liang, Caice / Ding, Sai / Sun, Wenjun / Liu, Li / Zhao, Wei / Zhang, Deli / Ying, Hanjie / Liu, Dong / Chen, Yong

Applied microbiology and biotechnology. 2020 Sept., v. 104, no. 17

2020

Abstract: Biofilm-based fermentation, as a new immobilisation strategy, is beneficial for industrial fermentation due to its excellent environmental resistance, high productivity and continuous fermentation relative to calcium alginate-immobilised fermentation. ... ...

Abstract	Biofilm-based fermentation, as a new immobilisation strategy, is beneficial for industrial fermentation due to its excellent environmental resistance, high productivity and continuous fermentation relative to calcium alginate-immobilised fermentation. These two techniques differ mainly regarding cell stages. Here, we describe the cell phenotype of Saccharomyces cerevisiae biofilm-based fermentation and compare cell cycle stages with those during immobilisation in calcium alginate. Most cells in the biofilm-based fermentation adhered to the cotton-fibre carrier of the biofilm and were in the G2/M phase whereas alginate-embedded cells were in the G1/G0 phase. Deletion of the RIM15 gene, which regulates cell cycle progression according to nutritional status, hampered the cell cycle arrest observed in alginate-embedded cells, enhanced biofilm formation and improved fermentation ability. The improved biofilm formation shown by the rim15△ strain could be attributed to an increase in the expression level of the adhesion protein FLO11 and synthesis of trehalose. These findings suggest that the extracellular environment is mainly responsible for the difference between biofilm-based fermentation and alginate-embedded fermentation, and that RIM15 plays an essential role in cell cycle progression. KEY POINTS: • In the biofilm, S. cerevisiae cell populations were mostly in the G2/M phase while alginate-embedded cells were arrested in the G1/G0 phase. • The RIM15 gene partially influenced the cell cycle progression observed during ethanol fermentation. • Biofilm-based cells were actively adsorbed on the physical carrier. • Biofilm immobilisation could maintain cell division activity explaining its fermentation efficiency.
Keywords	Saccharomyces cerevisiae ; adhesion ; biofilm ; calcium ; calcium alginate ; cell cycle checkpoints ; cell structures ; continuous fermentation ; ethanol fermentation ; ethanol production ; genes ; microbiology ; nutritional status ; trehalose
Language	English
Dates of publication	2020-09
Size	p. 7495-7505.
Publishing place	Springer Berlin Heidelberg
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	392453-1
ISSN	1432-0614 ; 0171-1741 ; 0175-7598
ISSN (online)	1432-0614
ISSN	0171-1741 ; 0175-7598
DOI	10.1007/s00253-020-10770-1
Database	NAL-Catalogue (AGRICOLA)

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