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  1. Article ; Online: Surveillance of endemic human Coronaviruses in Germany, 2019/2020

    Barbara Biere / Djin-Ye Oh / Thorsten Wolff / Ralf Dürrwald

    The Lancet Regional Health. Europe, Vol 11, Iss , Pp 100262- (2021)

    2021  

    Keywords Public aspects of medicine ; RA1-1270
    Language English
    Publishing date 2021-12-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Different populations of A(H1N1)pdm09 viruses in a patient with hemolytic-uremic syndrome

    Yuguang Fu / Marianne Wedde / Sigrun Smola / Djin-Ye Oh / Thorsten Pfuhl / Jürgen Rissland / Michael Zemlin / Fidelis A. Flockerzi / Rainer M. Bohle / Andrea Thürmer / Susanne Duwe / Barbara Biere / Janine Reiche / Brunhilde Schweiger / Christin Mache / Thorsten Wolff / Georg Herrler / Ralf Dürrwald

    International Journal of Medical Microbiology, Vol 314, Iss , Pp 151598- (2024)

    2024  

    Abstract: Respiratory viral infections may have different impacts ranging from infection without symptoms to severe disease or even death though the reasons are not well characterized.A patient (age group 5–15 years) displaying symptoms of hemolytic uremic ... ...

    Abstract Respiratory viral infections may have different impacts ranging from infection without symptoms to severe disease or even death though the reasons are not well characterized.A patient (age group 5–15 years) displaying symptoms of hemolytic uremic syndrome died one day after hospitalization. qPCR, next generation sequencing, virus isolation, antigenic characterization, resistance analysis was performed and virus replication kinetics in well-differentiated airway cells were determined.Autopsy revealed hemorrhagic pneumonia as major pathological manifestation. Lung samples harbored a large population of A(H1N1)pdm09 viruses with the polymorphism H456H/Y in PB1 polymerase. The H456H/Y viruses replicated much faster to high viral titers than upper respiratory tract viruses in vitro. H456H/Y-infected air-liquid interface cultures of differentiated airway epithelial cells did reflect a more pronounced loss of ciliated cells. A different pattern of virus quasispecies was found in the upper airway samples where substitution S263S/F (HA1) was observed.The data support the notion that viral quasispecies had evolved locally in the lung to support high replicative fitness. This change may have initiated further pathogenic processes leading to rapid dissemination of inflammatory mediators followed by development of hemorrhagic lung lesions and fatal outcome.
    Keywords A(H1N1)pdm09 virus ; Fatal influenza ; S263S/F (HA1) and H456H/Y (PB1) mutations ; Microbiology ; QR1-502 ; Other systems of medicine ; RZ201-999
    Language English
    Publishing date 2024-03-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Virological Surveillance and Molecular Characterization of Human Parainfluenzavirus Infection in Children with Acute Respiratory Illness

    Djin-Ye Oh / Barbara Biere / Markus Grenz / Thorsten Wolff / Brunhilde Schweiger / Ralf Dürrwald / Janine Reiche

    Microorganisms, Vol 9, Iss 1508, p

    Germany, 2015–2019

    2021  Volume 1508

    Abstract: Human parainfluenza viruses (HPIVs) are important causes of respiratory illness, especially in young children. However, surveillance for HPIV is rarely performed continuously, and national-level epidemiologic and genetic data are scarce. Within the ... ...

    Abstract Human parainfluenza viruses (HPIVs) are important causes of respiratory illness, especially in young children. However, surveillance for HPIV is rarely performed continuously, and national-level epidemiologic and genetic data are scarce. Within the German sentinel system, to monitor acute respiratory infections (ARI), 4463 respiratory specimens collected from outpatients < 5 years of age between October 2015 and September 2019 were retrospectively screened for HPIV 1–4 using real-time PCR. HPIV was identified in 459 (10%) samples. HPIV-3 was the most common HPIV-type, with 234 detections, followed by HPIV-1 (113), HPIV-4 (61), and HPIV-2 (49). HPIV-3 was more frequently associated with age < 2 years, and HPIV-4 was more frequently associated with pneumonia compared to other HPIV types. HPIV circulation displayed distinct seasonal patterns, which appeared to vary by type. Phylogenetic characterization clustered HPIV-1 in Clades 2 and 3. Reclassification was performed for HPIV-2, provisionally assigning two distinct HPIV-2 groups and six clades, with German HPIV-2s clustering in Clade 2.4. HPIV-3 clustered in C1, C3, C5, and, interestingly, in A. HPIV-4 clustered in Clades 2.1 and 2.2. The results of this study may serve to inform future approaches to diagnose and prevent HPIV infections, which contribute substantially to ARI in young children in Germany.
    Keywords human parainfluenza virus ; orthorubulavirus ; respirovirus ; acute respiratory infection ; influenza-like illness ; croup ; Biology (General) ; QH301-705.5
    Subject code 572
    Language English
    Publishing date 2021-07-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: SARS-CoV-2 Omicron variant is attenuated for replication in a polarized human lung epithelial cell model

    Christin Mache / Jessica Schulze / Gudrun Holland / Daniel Bourquain / Jean-Marc Gensch / Djin-Ye Oh / Andreas Nitsche / Ralf Dürrwald / Michael Laue / Thorsten Wolff

    Communications Biology, Vol 5, Iss 1, Pp 1-

    2022  Volume 8

    Abstract: Human alveolar epithelial lentivirus immortalized cells are very permissive for human coronaviruses and influenza A viruses, provided a suitable model for such infections of the lower respiratory tract as shown by reduced propagation of the SARS-CoV-2 ... ...

    Abstract Human alveolar epithelial lentivirus immortalized cells are very permissive for human coronaviruses and influenza A viruses, provided a suitable model for such infections of the lower respiratory tract as shown by reduced propagation of the SARS-CoV-2 Omicron variant.
    Keywords Biology (General) ; QH301-705.5
    Language English
    Publishing date 2022-10-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Trends in respiratory virus circulation following COVID-19-targeted nonpharmaceutical interventions in Germany, January - September 2020

    Djin-Ye Oh / Silke Buda / Barbara Biere / Janine Reiche / Frank Schlosser / Susanne Duwe / Marianne Wedde / Max von Kleist / Martin Mielke / Thorsten Wolff / Ralf Dürrwald

    The Lancet Regional Health. Europe, Vol 6, Iss , Pp 100112- (2021)

    Analysis of national surveillance data

    2021  

    Abstract: Background: During the initial COVID-19 response, Germany's Federal Government implemented several nonpharmaceutical interventions (NPIs) that were instrumental in suppressing early exponential spread of SARS-CoV-2. NPI effect on the transmission of ... ...

    Abstract Background: During the initial COVID-19 response, Germany's Federal Government implemented several nonpharmaceutical interventions (NPIs) that were instrumental in suppressing early exponential spread of SARS-CoV-2. NPI effect on the transmission of other respiratory viruses has not been examined at the national level thus far. Methods: Upper respiratory tract specimens from 3580 patients with acute respiratory infection (ARI), collected within the nationwide German ARI Sentinel, underwent RT-PCR diagnostics for multiple respiratory viruses. The observation period (weeks 1-38 of 2020) included the time before, during and after a far-reaching contact ban. Detection rates for different viruses were compared to 2017-2019 sentinel data (15350 samples; week 1-38, 11823 samples). Findings: The March 2020 contact ban, which was followed by a mask mandate, was associated with an unprecedented and sustained decline of multiple respiratory viruses. Among these, rhinovirus was the single agent that resurged to levels equalling those of previous years. Rhinovirus rebound was first observed in children, after schools and daycares had reopened. By contrast, other nonenveloped viruses (i.e. gastroenteritis viruses reported at the national level) suppressed after the shutdown did not rebound. Interpretation: Contact restrictions with a subsequent mask mandate in spring may substantially reduce respiratory virus circulation. This reduction appears sustained for most viruses, indicating that the activity of influenza and other respiratory viruses during the subsequent winter season might be low,whereas rhinovirus resurgence, potentially driven by transmission in educational institutions in a setting of waning population immunity, might signal predominance of rhinovirus-related ARIs. Funding: Robert Koch-Institute and German Ministry of Health.
    Keywords Nonpharmaceutical interventions ; SARS-CoV-2 ; Respiratory virus ; Rhinovirus ; Surveillance ; Public aspects of medicine ; RA1-1270
    Language English
    Publishing date 2021-07-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: 2549 Characterizing the expression kinetics of HIV-1 envelope protein

    Djin-Ye Oh / Lihong Liu / Benjamin Trinité / En-Wei Hu-Van Wright / Vincent Sahi / Yaoxing Huang / David N. Levy / David D. Ho

    Journal of Clinical and Translational Science, Vol 2, Pp 7-

    2018  Volume 7

    Abstract: OBJECTIVES/SPECIFIC AIMS: Characterize the expression kinetics of HIV-1 Envelope and their relationship to virus production at the cellular level. METHODS/STUDY POPULATION: In vitro and ex vivo laboratory analyses. RESULTS/ANTICIPATED RESULTS: Initial ... ...

    Abstract OBJECTIVES/SPECIFIC AIMS: Characterize the expression kinetics of HIV-1 Envelope and their relationship to virus production at the cellular level. METHODS/STUDY POPULATION: In vitro and ex vivo laboratory analyses. RESULTS/ANTICIPATED RESULTS: Initial studies addressing the kinetics of cell surface. Envelope (Env) expression reveal that Env expression to peaks on day 2 post infection. Next steps include a series of experiments to compare the kinetics of Env cell surface expression with broadly neutralizing antibody (bNAb)-mediated ADCC and the characterization of virus production kinetics in this same context. To be maximally effective, ADCC elimination of infected cells should occur before peak Env expression. DISCUSSION/SIGNIFICANCE OF IMPACT: Potent bNAbs to HIV-1 recognize vulnerable sites on the HIV-1 Envelope (Env) protein and are of great clinical interest due to their potential use in the prevention and treatment of HIV-1 infection. Their effectiveness depends not only on the neutralization of viral infectivity, but also on the elimination of productively infected cells via antibody-dependent cellular cytotoxicity (ADCC). On a cellular level, ADCC dynamics are determined by the timing and level of Env expression on the surface of HIV-infected cells. This study aims to delineate the expression kinetics of HIV-1 Envelope and their relationship to virus production. We expect that it will provide new insights into the utility of bNAb-mediated ADCC in treating and possibly curing HIV-1 infection; therefore results might have substantial impact on future HIV treatment strategies.
    Keywords Medicine ; R
    Subject code 570
    Language English
    Publishing date 2018-06-01T00:00:00Z
    Publisher Cambridge University Press
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: CCR5Delta32 genotypes in a German HIV-1 seroconverter cohort and report of HIV-1 infection in a CCR5Delta32 homozygous individual.

    Djin-Ye Oh / Heiko Jessen / Claudia Kücherer / Konrad Neumann / Nari Oh / Gabriele Poggensee / Barbara Bartmeyer / Arne Jessen / Axel Pruss / Ralf R Schumann / Osamah Hamouda

    PLoS ONE, Vol 3, Iss 7, p e

    2008  Volume 2747

    Abstract: BACKGROUND: Homozygosity (Delta32/Delta32) for the 32 bp deletion in the chemokine receptor 5 (CCR5) gene is associated with strong resistance against HIV infection. Heterozygosity is associated with protection of HIV-1 disease progression. METHODOLOGY/ ... ...

    Abstract BACKGROUND: Homozygosity (Delta32/Delta32) for the 32 bp deletion in the chemokine receptor 5 (CCR5) gene is associated with strong resistance against HIV infection. Heterozygosity is associated with protection of HIV-1 disease progression. METHODOLOGY/PRINCIPAL FINDINGS: We genotyped a population of 737 HIV-positive adults and 463 healthy controls for the CCR5Delta32 deletion and found heterozygous frequencies of 16.2% (HIV-negative) and 17.5% (HIV-positive) among Caucasian individuals. Analysis of CCR5Delta32 influence on disease progression showed notably lower viral setpoints and a longer time to a CD4 count of <200 microl(-1) in seroconverters heterozygous for the deletion. Furthermore, we identified one HIV-positive man homozygous for the Delta32 deletion. CONCLUSIONS/SIGNIFICANCE: The protective effect of CCR5 Delta32 heterozygosity is confirmed in a large cohort of German seroconverters. The HIV-infected CCR5 Delta32 homozygous individual, however, displays extremely rapid disease progression. This is the 12th case of HIV-infection in this genotype described worldwide.
    Keywords Medicine ; R ; Science ; Q
    Language English
    Publishing date 2008-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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