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  1. Article ; Online: Aneurysmal 99m Tc-HMPAO Uptake in Brain Death.

    Do, Danka / Suhaj, Petr / Benes, Jiri / Matej, Radoslav / Lang, Otto

    Clinical nuclear medicine

    2024  Volume 49, Issue 4, Page(s) 348–350

    Abstract: Abstract: A 41-year-old man was admitted to hospital due to sudden loss of consciousness. A regional brain perfusion SPECT/low-dose CT showed abnormal 99m Tc-HMPAO uptake in the right hemisphere frontotemporally without any other supratentorial or ... ...

    Abstract Abstract: A 41-year-old man was admitted to hospital due to sudden loss of consciousness. A regional brain perfusion SPECT/low-dose CT showed abnormal 99m Tc-HMPAO uptake in the right hemisphere frontotemporally without any other supratentorial or infratentorial radiotracer uptake. A neuropathological examination disclosed a middle cerebral artery aneurysm. Presumably, vessel wall fibrosis prevented collapse. Multiple transmural dissections of the fibrotic aneurysmal wall were the source of the subarachnoid hemorrhage. This interesting image shows that radiotracer accumulation in cerebral artery aneurysms can be a diagnostic pitfall in brain death scintigraphy assessment.
    MeSH term(s) Male ; Humans ; Adult ; Brain Death/diagnostic imaging ; Brain/diagnostic imaging ; Biological Transport ; Neuroimaging ; Single Photon Emission Computed Tomography Computed Tomography
    Language English
    Publishing date 2024-02-05
    Publishing country United States
    Document type Case Reports ; Journal Article
    ZDB-ID 197628-x
    ISSN 1536-0229 ; 0363-9762
    ISSN (online) 1536-0229
    ISSN 0363-9762
    DOI 10.1097/RLU.0000000000005103
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  2. Article: Herbarium tale: the utility of dry specimens for DNA barcoding Juncaceae

    Do, Danka / Lenka Záveská Drábková

    Plant systematics and evolution. 2018 Feb., v. 304, no. 2

    2018  

    Abstract: Phylogenetic and barcoding studies usually use fresh plant tissues as sources of DNA and have successfully amplified DNA for various loci. The use of dried samples, however, is often necessary due to the frequent inaccessibility of fresh rare plants or ... ...

    Abstract Phylogenetic and barcoding studies usually use fresh plant tissues as sources of DNA and have successfully amplified DNA for various loci. The use of dried samples, however, is often necessary due to the frequent inaccessibility of fresh rare plants or their parts for genetic analyses or barcoding. The difficulty in obtaining amplifiable DNA is a major restriction of the use of herbarium specimens for DNA analyses. Recent study has highlighted the crucial issues for comparing herbarium and fresh plants for barcoding. We analysed the performance of samples of the family Juncaceae from various herbarium specimens of different ages with fresh plant material in PCRs and the sequences of seven loci (rbcL, rpoC1, trnL-F intergenic spacer, trnL intron, and psbA-trnH from chloroplast DNA; atp1 from mitochondrial DNA; and ITS1-5.8S-ITS2 from nuclear DNA) using a combination of 28 primers. The herbarium specimens amplified well and may thus be successfully applied for both phylogenetic analyses and barcoding for the Juncaceae family. Amplifying DNA was more difficult from dried herbarium specimens than fresh samples but could be successful in most cases when appropriate internal primers were designed or methods were optimised. Using the set of universal primers recommended by the Consortium for the Barcode of Life and designing specific primers for a particular group of interest were both useful. Specimen age and amplicon length had limited detrimental effects on amplification success for most of the Juncaceae loci tested.
    Keywords DNA barcoding ; Juncaceae ; barcoding ; chloroplast DNA ; genetic analysis ; herbaria ; intergenic DNA ; internal transcribed spacers ; introns ; loci ; mitochondrial DNA ; nuclear genome ; phylogeny ; plant tissues ; plants (botany) ; polymerase chain reaction ; rare species
    Language English
    Dates of publication 2018-02
    Size p. 281-294.
    Publishing place Springer Vienna
    Document type Article
    ZDB-ID 1463027-8
    ISSN 1615-6110 ; 0378-2697
    ISSN (online) 1615-6110
    ISSN 0378-2697
    DOI 10.1007/s00606-017-1476-x
    Database NAL-Catalogue (AGRICOLA)

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