LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 66

Search options

  1. Article ; Online: Epigenetics in

    Wang, Yinuo / Dobreva, Gergana

    Cells

    2023  Volume 12, Issue 5

    Abstract: Mutations in the gene for lamin A/ ... ...

    Abstract Mutations in the gene for lamin A/C
    MeSH term(s) Animals ; Mice ; Cardiomyopathies/genetics ; Chromatin ; Epigenesis, Genetic ; Heart ; Lamin Type A/metabolism ; Humans
    Chemical Substances Chromatin ; Lamin Type A ; LMNA protein, human
    Language English
    Publishing date 2023-03-01
    Publishing country Switzerland
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells12050783
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: Human pluripotent stem cell-based models of heart development and disease.

    Velichkova, Gabriel / Dobreva, Gergana

    Cells & development

    2023  Volume 175, Page(s) 203857

    Abstract: The heart is a complex organ composed of distinct cell types, such as cardiomyocytes, cardiac fibroblasts, endothelial cells, smooth muscle cells, neuronal cells and immune cells. All these cell types contribute to the structural, electrical and ... ...

    Abstract The heart is a complex organ composed of distinct cell types, such as cardiomyocytes, cardiac fibroblasts, endothelial cells, smooth muscle cells, neuronal cells and immune cells. All these cell types contribute to the structural, electrical and mechanical properties of the heart. Genetic manipulation and lineage tracing studies in mice have been instrumental in gaining critical insights into the networks regulating cardiac cell lineage specification, cell fate and plasticity. Such knowledge has been of fundamental importance for the development of efficient protocols for the directed differentiation of pluripotent stem cells (PSCs) in highly specialized cardiac cell types. In this review, we summarize the evolution and current advances in protocols for cardiac subtype specification, maturation, and assembly in cardiac microtissues and organoids.
    MeSH term(s) Humans ; Mice ; Animals ; Endothelial Cells ; Pluripotent Stem Cells/metabolism ; Myocytes, Cardiac/metabolism ; Cell Differentiation/genetics ; Fibroblasts
    Language English
    Publishing date 2023-05-29
    Publishing country Netherlands
    Document type Review ; Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2667-2901
    ISSN (online) 2667-2901
    DOI 10.1016/j.cdev.2023.203857
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article: The LINC Between Mechanical Forces and Chromatin.

    Lityagina, Olga / Dobreva, Gergana

    Frontiers in physiology

    2021  Volume 12, Page(s) 710809

    Abstract: The heart continually senses and responds to mechanical stimuli that balance cardiac structure and activity. Tensile forces, compressive forces, and shear stress are sensed by the different cardiac cell types and converted into signals instructing proper ...

    Abstract The heart continually senses and responds to mechanical stimuli that balance cardiac structure and activity. Tensile forces, compressive forces, and shear stress are sensed by the different cardiac cell types and converted into signals instructing proper heart morphogenesis, postnatal growth, and function. Defects in mechanotransduction, the ability of cells to convert mechanical stimuli into biochemical signals, are implicated in cardiovascular disease development and progression. In this review, we summarize the current knowledge on how mechanical forces are transduced to chromatin through the tensed actomyosin cytoskeleton, the linker of nucleoskeleton and cytoskeleton (LINC) complex and the nuclear lamina. We also discuss the functional significance of the LINC complex in cardiovascular disease.
    Language English
    Publishing date 2021-08-02
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2564217-0
    ISSN 1664-042X
    ISSN 1664-042X
    DOI 10.3389/fphys.2021.710809
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article ; Online: Epigenetic memory of cell fate commitment.

    Elsherbiny, Adel / Dobreva, Gergana

    Current opinion in cell biology

    2021  Volume 69, Page(s) 80–87

    Abstract: During development, discrete cell fates are established in precise spatiotemporal order guided by morphogen signals. These signals converge in the nucleus to induce transcriptional and epigenetic programming that determines cell fate. Once cell identity ... ...

    Abstract During development, discrete cell fates are established in precise spatiotemporal order guided by morphogen signals. These signals converge in the nucleus to induce transcriptional and epigenetic programming that determines cell fate. Once cell identity is established, cell programs have to be accurately sustained through multiple rounds of cell division, during which DNA replication serves as a window of opportunity for altering cell fate. In this review, we summarize recent advances in understanding the molecular players that underlie epigenetic memory of cell fate decisions, with a particular focus on histone modifications and mitotic bookmarking factors. We also discuss the different mechanisms of inheritance of repressed and active chromatin states.
    MeSH term(s) Cell Differentiation ; Cell Plasticity ; Chromatin ; Chromatin Assembly and Disassembly ; Epigenesis, Genetic ; Histones/metabolism ; Humans
    Chemical Substances Chromatin ; Histones
    Language English
    Publishing date 2021-01-31
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 1026381-0
    ISSN 1879-0410 ; 0955-0674
    ISSN (online) 1879-0410
    ISSN 0955-0674
    DOI 10.1016/j.ceb.2020.12.014
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 2963: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article ; Online: S-adenosylmethionine treatment affects histone methylation in prostate cancer cells.

    Mathes, Arthur / Duman, Merve Busra / Neumann, Alexander / Dobreva, Gergana / Schmidt, Thomas

    Gene

    2023  Volume 893, Page(s) 147915

    Abstract: S-adenosylmethionine (SAM) represents a potent inhibitor of cancer cell proliferation, migration, and invasionin vitro.The underlying mechanisms remain elusive. Here, we examined, if treatment with SAM may cause alterations in the methylation of the ... ...

    Abstract S-adenosylmethionine (SAM) represents a potent inhibitor of cancer cell proliferation, migration, and invasionin vitro.The underlying mechanisms remain elusive. Here, we examined, if treatment with SAM may cause alterations in the methylation of the histone marks H3K4me3 and H3K27me3, which are both known to play important roles in the initiation and progression of prostate cancer. We treated PC-3 cells with 200 µmol SAM, a concentration known to cause anticancerogenic effects, followed by ChIP-sequencing for H3K4me3 and H3K27me3. We detected 236 differentially methylated regions for H3K27me3 and 560 differentially methylated regions for H3K4me3. GO Term enrichment showed upregulation of anticancerogenic, as well as downregulation of cancerogenic related biological processes, molecular functions, and pathways. Furthermore, we compared specific methylation profiles of SAM treated samples to gene expression changes (RNA-Seq). 35 upregulated and 56 downregulated genes (total: 604 differentially expressed genes) could be related to hypomethylated and hypermethylated regions. 17 upregulated genes could be identified as tumor suppressor genes, 45 downregulated genes in contrast are considered as oncogenes. As a conclusion it can be stated that SAM treatment of prostate cancer cells resulted in alterations of H3K4me3 and H3K27me3 methylation profiles. Gene to peak annotation, alignment with results of a transcriptome study as well as GO-term analysis underpinned the biological relevance of methylation changes.
    MeSH term(s) Male ; Humans ; Methylation ; Histones/metabolism ; S-Adenosylmethionine/pharmacology ; S-Adenosylmethionine/metabolism ; Prostatic Neoplasms/drug therapy ; Prostatic Neoplasms/genetics ; Prostatic Neoplasms/pathology ; Protein Processing, Post-Translational ; DNA Methylation
    Chemical Substances Histones ; S-Adenosylmethionine (7LP2MPO46S)
    Language English
    Publishing date 2023-10-20
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 391792-7
    ISSN 1879-0038 ; 0378-1119
    ISSN (online) 1879-0038
    ISSN 0378-1119
    DOI 10.1016/j.gene.2023.147915
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 1357: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  6. Article ; Online: Role of endothelial PDGFB in arterio-venous malformations pathogenesis.

    Lin, Yanzhu / Gahn, Johannes / Banerjee, Kuheli / Dobreva, Gergana / Singhal, Mahak / Dubrac, Alexandre / Ola, Roxana

    Angiogenesis

    2023  Volume 27, Issue 2, Page(s) 193–209

    Abstract: Arterial-venous malformations (AVMs) are direct connections between arteries and veins without an intervening capillary bed. Either familial inherited or sporadically occurring, localized pericytes (PCs) drop is among the AVMs' hallmarks. Whether ... ...

    Abstract Arterial-venous malformations (AVMs) are direct connections between arteries and veins without an intervening capillary bed. Either familial inherited or sporadically occurring, localized pericytes (PCs) drop is among the AVMs' hallmarks. Whether impaired PC coverage triggers AVMs or it is a secondary event is unclear. Here we evaluated the role of the master regulator of PC recruitment, Platelet derived growth factor B (PDGFB) in AVM pathogenesis. Using tamoxifen-inducible deletion of Pdgfb in endothelial cells (ECs), we show that disruption of EC Pdgfb-mediated PC recruitment and maintenance leads to capillary enlargement and organotypic AVM-like structures. These vascular lesions contain non-proliferative hyperplastic, hypertrophic and miss-oriented capillary ECs with an altered capillary EC fate identity. Mechanistically, we propose that PDGFB maintains capillary EC size and caliber to limit hemodynamic changes, thus restricting expression of Krüppel like factor 4 and activation of Bone morphogenic protein, Transforming growth factor β and NOTCH signaling in ECs. Furthermore, our study emphasizes that inducing or activating PDGFB signaling may be a viable therapeutic approach for treating vascular malformations.
    MeSH term(s) Humans ; Proto-Oncogene Proteins c-sis/metabolism ; Endothelial Cells/metabolism ; Vascular Diseases/metabolism ; Capillaries/metabolism ; Pericytes/metabolism
    Chemical Substances Proto-Oncogene Proteins c-sis
    Language English
    Publishing date 2023-12-09
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1484717-6
    ISSN 1573-7209 ; 0969-6970
    ISSN (online) 1573-7209
    ISSN 0969-6970
    DOI 10.1007/s10456-023-09900-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 5094: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  7. Article ; Online: A BMP4-p38 MAPK signaling axis controls ISL1 protein stability and activity during cardiogenesis.

    Jing, Yanyan / Ren, Yonggang / Witzel, Hagen Roland / Dobreva, Gergana

    Stem cell reports

    2021  Volume 16, Issue 8, Page(s) 1894–1905

    Abstract: During development, cells respond rapidly to intra- and intercellular signals, which induce signaling cascades regulating the activity of transcription factors at the transcriptional and/or post-translational level. The transcription factor ISL1 plays a ... ...

    Abstract During development, cells respond rapidly to intra- and intercellular signals, which induce signaling cascades regulating the activity of transcription factors at the transcriptional and/or post-translational level. The transcription factor ISL1 plays a key role in second heart field development and cardiac differentiation, and its mRNA levels are tightly regulated during cardiogenesis. Here, we show that a BMP-p38 MAPK signaling axis controls ISL1 protein function at the post-translational level. BMP-mediated activation of p38 MAPK leads to ISL1 phosphorylation at S269 by p38, which prevents ISL1 degradation and ensures its transcriptional activity during cardiogenesis. Interfering with p38 MAPK signaling leads to the degradation of ISL1 by the proteasome, resulting in defects in cardiomyocyte differentiation and impaired zebrafish and mouse heart morphogenesis and function. Given the critical role of the tight control of ISL1 activity during cardiac lineage diversification, modulation of BMP4-p38 MAPK signaling could direct differentiation into specific cardiac cell subpopulations.
    MeSH term(s) Animals ; Animals, Genetically Modified ; Bone Morphogenetic Protein 4/metabolism ; Cell Differentiation/genetics ; Gene Expression Regulation, Developmental ; Heart/embryology ; LIM-Homeodomain Proteins/genetics ; LIM-Homeodomain Proteins/metabolism ; Mice ; Mice, Knockout ; Myocardium/cytology ; Myocardium/metabolism ; NIH 3T3 Cells ; Organogenesis/genetics ; Protein Stability ; Signal Transduction ; Stem Cells/metabolism ; Transcription Factors/genetics ; Transcription Factors/metabolism ; Zebrafish/embryology ; Zebrafish/genetics ; Zebrafish/metabolism ; p38 Mitogen-Activated Protein Kinases/metabolism
    Chemical Substances Bone Morphogenetic Protein 4 ; LIM-Homeodomain Proteins ; Transcription Factors ; insulin gene enhancer binding protein Isl-1 ; p38 Mitogen-Activated Protein Kinases (EC 2.7.11.24)
    Language English
    Publishing date 2021-07-29
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2720528-9
    ISSN 2213-6711 ; 2213-6711
    ISSN (online) 2213-6711
    ISSN 2213-6711
    DOI 10.1016/j.stemcr.2021.06.017
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  8. Article ; Online: Publisher Correction: Nascent Ribo-Seq measures ribosomal loading time and reveals kinetic impact on ribosome density.

    Schott, Johanna / Reitter, Sonja / Lindner, Doris / Grosser, Jan / Bruer, Marius / Shenoy, Anjana / Geiger, Tamar / Mathes, Arthur / Dobreva, Gergana / Stoecklin, Georg

    Nature methods

    2023  Volume 20, Issue 2, Page(s) 316

    Language English
    Publishing date 2023-01-19
    Publishing country United States
    Document type Published Erratum
    ZDB-ID 2169522-2
    ISSN 1548-7105 ; 1548-7091
    ISSN (online) 1548-7105
    ISSN 1548-7091
    DOI 10.1038/s41592-023-01765-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 6022: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  9. Article ; Online: Lamin B1 in cancer and aging.

    Garvalov, Boyan K / Muhammad, Sajjad / Dobreva, Gergana

    Aging

    2019  Volume 11, Issue 18, Page(s) 7336–7338

    MeSH term(s) Aging/physiology ; Animals ; Enhancer of Zeste Homolog 2 Protein/genetics ; Enhancer of Zeste Homolog 2 Protein/metabolism ; Gene Deletion ; Gene Expression Regulation ; Lamin Type B/genetics ; Lamin Type B/metabolism ; Mice ; Neoplasms/metabolism ; Polycomb Repressive Complex 2/genetics ; Polycomb Repressive Complex 2/metabolism ; Proto-Oncogene Proteins c-ret/genetics ; Proto-Oncogene Proteins c-ret/metabolism
    Chemical Substances Lamin Type B ; Enhancer of Zeste Homolog 2 Protein (EC 2.1.1.43) ; Ezh1 protein, mouse (EC 2.1.1.43) ; Ezh2 protein, mouse (EC 2.1.1.43) ; Polycomb Repressive Complex 2 (EC 2.1.1.43) ; Proto-Oncogene Proteins c-ret (EC 2.7.10.1) ; Ret protein, mouse (EC 2.7.10.1)
    Language English
    Publishing date 2019-09-20
    Publishing country United States
    Document type Editorial
    ISSN 1945-4589
    ISSN (online) 1945-4589
    DOI 10.18632/aging.102306
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  10. Article ; Online: Leveraging chromatin state transitions for the identification of regulatory networks orchestrating heart regeneration.

    Cordero, Julio / Elsherbiny, Adel / Wang, Yinuo / Jürgensen, Lonny / Constanty, Florian / Günther, Stefan / Boerries, Melanie / Heineke, Joerg / Beisaw, Arica / Leuschner, Florian / Hassel, David / Dobreva, Gergana

    Nucleic acids research

    2024  Volume 52, Issue 8, Page(s) 4215–4233

    Abstract: The limited regenerative capacity of the human heart contributes to high morbidity and mortality worldwide. In contrast, zebrafish exhibit robust regenerative capacity, providing a powerful model for studying how to overcome intrinsic epigenetic barriers ...

    Abstract The limited regenerative capacity of the human heart contributes to high morbidity and mortality worldwide. In contrast, zebrafish exhibit robust regenerative capacity, providing a powerful model for studying how to overcome intrinsic epigenetic barriers maintaining cardiac homeostasis and initiate regeneration. Here, we present a comprehensive analysis of the histone modifications H3K4me1, H3K4me3, H3K27me3 and H3K27ac during various stages of zebrafish heart regeneration. We found a vast gain of repressive chromatin marks one day after myocardial injury, followed by the acquisition of active chromatin characteristics on day four and a transition to a repressive state on day 14, and identified distinct transcription factor ensembles associated with these events. The rapid transcriptional response involves the engagement of super-enhancers at genes implicated in extracellular matrix reorganization and TOR signaling, while H3K4me3 breadth highly correlates with transcriptional activity and dynamic changes at genes involved in proteolysis, cell cycle activity, and cell differentiation. Using loss- and gain-of-function approaches, we identified transcription factors in cardiomyocytes and endothelial cells influencing cardiomyocyte dedifferentiation or proliferation. Finally, we detected significant evolutionary conservation between regulatory regions that drive zebrafish and neonatal mouse heart regeneration, suggesting that reactivating transcriptional and epigenetic networks converging on these regulatory elements might unlock the regenerative potential of adult human hearts.
    MeSH term(s) Zebrafish/genetics ; Animals ; Regeneration/genetics ; Chromatin/metabolism ; Chromatin/genetics ; Histones/metabolism ; Heart ; Myocytes, Cardiac/metabolism ; Myocytes, Cardiac/cytology ; Gene Regulatory Networks ; Mice ; Humans ; Epigenesis, Genetic ; Transcription Factors/metabolism ; Transcription Factors/genetics ; Histone Code ; Cell Differentiation/genetics
    Chemical Substances Chromatin ; Histones ; Transcription Factors
    Language English
    Publishing date 2024-02-15
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
    ZDB-ID 186809-3
    ISSN 1362-4962 ; 1362-4954 ; 0301-5610 ; 0305-1048
    ISSN (online) 1362-4962 ; 1362-4954
    ISSN 0301-5610 ; 0305-1048
    DOI 10.1093/nar/gkae085
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 1067: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top