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  1. Article ; Online: The Impact of the Female Genital Microbiota on the Outcome of Assisted Reproduction Treatments

    Giovanna Cocomazzi / Silvia De Stefani / Lino Del Pup / Simone Palini / Matteo Buccheri / Mariangela Primiterra / Natale Sciannamè / Raffaele Faioli / Annamaria Maglione / Giorgio Maria Baldini / Domenico Baldini / Valerio Pazienza

    Microorganisms, Vol 11, Iss 1443, p

    2023  Volume 1443

    Abstract: The vaginal microbiota plays a critical role in the health of the female genital tract, and its composition contributes to gynecological disorders and infertility. Lactobacilli are the dominant species in the female genital tract: their production of ... ...

    Abstract The vaginal microbiota plays a critical role in the health of the female genital tract, and its composition contributes to gynecological disorders and infertility. Lactobacilli are the dominant species in the female genital tract: their production of lactic acid, hydrogen peroxide, and bacteriocins prevents the invasion and growth of pathogenic microorganisms. Several factors such as hormonal changes, age of reproduction, sexual practices, menstrual cycle, pregnancy, and antimicrobial drugs use can cause imbalance and dysbiosis of the vaginal microbiota. This review aims to highlight the impact of the vaginal microbiota in Assisted Reproductive Technology techniques (ART) and it examines the factors that influence the vaginal microbiota, the consequences of dysbiosis, and potential interventions to restore a healthy female genital tract.
    Keywords vaginal microbiota ; bacterial vaginosis ; assisted reproduction technologies ; endometriosis ; infertility ; pregnancy outcome ; Biology (General) ; QH301-705.5
    Subject code 630
    Language English
    Publishing date 2023-05-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Sperm Selection for ICSI

    Domenico Baldini / Daniele Ferri / Giorgio Maria Baldini / Dario Lot / Assunta Catino / Damiano Vizziello / Giovanni Vizziello

    Cells, Vol 10, Iss 3566, p

    Do We Have a Winner?

    2021  Volume 3566

    Abstract: In assisted reproductive technology (ART), the aim of sperm cells’ preparation is to select competent spermatozoa with the highest fertilization potential and in this context, the intracytoplasmic sperm injection (ICSI) represents the most applied ... ...

    Abstract In assisted reproductive technology (ART), the aim of sperm cells’ preparation is to select competent spermatozoa with the highest fertilization potential and in this context, the intracytoplasmic sperm injection (ICSI) represents the most applied technique for fertilization. This makes the process of identifying the perfect spermatozoa extremely important. A number of methods have now been developed to mimic some of the natural selection processes that exist in the female reproductive tract. Although many studies have been conducted to identify the election technique, many doubts and disagreements still remain. In this review, we will discuss all the sperm cell selection techniques currently available for ICSI, starting from the most basic methodologies and continuing with those techniques suitable for sperm cells with reduced motility. Furthermore, different techniques that exploit some sperm membrane characteristics and the most advanced strategy for sperm selection based on microfluidics, will be examined. Finally, a new sperm selection method based on a micro swim-up directly on the ICSI dish will be analyzed. Eventually, advantages and disadvantages of each technique will be debated, trying to draw reasonable conclusions on their efficacy in order to establish the gold standard method.
    Keywords assisted reproductive technology (ART) ; in vitro fertilization (IVF) ; intracytoplasmic sperm injection (ICSI) ; sperm selection ; density gradient (DGC) ; swim-up (SU) ; Biology (General) ; QH301-705.5
    Subject code 621
    Language English
    Publishing date 2021-12-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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