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Article ; Online: The immune response to a Coxiella burnetii vaccine in sheep varies according to their natural pre-exposure.

Böttcher, Jens / Bauer, Benjamin U / Ambros, Christina / Alex, Michaela / Domes, Ursula / Roth, Sabine / Boll, Kerstin / Korneli, Martin / Bogner, Karl-Heinz / Randt, Andreas / Janowetz, Britta

Vaccine

2024 Volume 42, Issue 8, Page(s) 1993–2003

Abstract: Q fever in humans is caused by Coxiella (C.) burnetii. In 2008 and 2012, cases of Q fever in humans were linked to an infected flock of approximately 650 ewes. Since 2013 gimmers (G'13, G'14, G'15 etc.) were primary vaccinated (two doses) with an ... ...

Abstract	Q fever in humans is caused by Coxiella (C.) burnetii. In 2008 and 2012, cases of Q fever in humans were linked to an infected flock of approximately 650 ewes. Since 2013 gimmers (G'13, G'14, G'15 etc.) were primary vaccinated (two doses) with an inactivated C.burnetii vaccine without any revaccination. In 2013, 30 ewes were primary vaccinated (A'13). Shedding was annually monitored by qPCR-testing of vaginal and nasal swabs collected at lambing. Animals were tested for Phase I- (PhI) and PhII-antibodies (Ab) and for PhII-specific-interferon-γ (IFN-γ) before and after vaccination. The effect of a revaccination was determined in 2018 and 2023. Groups of randomly selected gimmers primary vaccinated in 2015, 2016 and 2017 and a mixed group of older animals (A'13, G'13 and G'14) were revaccinated once in 2018. The trial was repeated in 2023 on groups primary vaccinated in 2019-2023. Major shedding after the outbreak in 2012 ceased in 2014. Thereafter C.burnetii was only sporadically detected at low-level in 2018, 2021 and 2023. Sheep naturally exposed to C.burnetii during the outbreak in 2012 (A'13, G'13) mounted a strong and complete (PhI, PhII, IFN-γ) recall immune response after vaccination. A serological PhI
MeSH term(s)	Humans ; Sheep ; Animals ; Female ; Coxiella burnetii ; Q Fever/prevention & control ; Q Fever/veterinary ; Q Fever/epidemiology ; Antibodies ; Bacterial Vaccines ; Immunity
Chemical Substances	Antibodies ; Bacterial Vaccines
Language	English
Publishing date	2024-02-21
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	605674-x
ISSN	1873-2518 ; 0264-410X
ISSN (online)	1873-2518
ISSN	0264-410X
DOI	10.1016/j.vaccine.2024.02.048
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Long-term control of Coxiellosis in sheep by annual primary vaccination of gimmers

Böttcher, Jens / Bauer, Benjamin U. / Ambros, Christina / Alex, Michaela / Domes, Ursula / Roth, Sabine / Boll, Kerstin / Korneli, Martin / Bogner, Karl-Heinz / Randt, Andreas / Janowetz, Britta

Vaccine. 2022 July 21,

2022

Abstract: Coxiella (C.) burnetii, a Gram-negative intracellular bacterium, causes Q fever in humans and Coxiellosis in animals. Ruminants are a primary source of human infection with C.burnetii. In 2013, vaccination was implemented in a sheep flock with 650 ewes ... ...

Abstract	Coxiella (C.) burnetii, a Gram-negative intracellular bacterium, causes Q fever in humans and Coxiellosis in animals. Ruminants are a primary source of human infection with C.burnetii. In 2013, vaccination was implemented in a sheep flock with 650 ewes associated with two outbreaks of Q fever in humans in 2008 and 2012. Only gimmers (yearlings) received two doses of a commercial C.burnetii phase I whole cell vaccine three weeks apart (primary vaccination) without any revaccination. Vaginal and nasal swabs collected shortly after lambing were tested by qPCR. Additionally, a group of non-vaccinated sentinels was serologically monitored for phase I (PhI), II (PhII) antibodies and for Interferon γ (IFN-γ) after stimulation of whole blood cells with PhII-antigen with and without an IL-10-neutralizing monoclonal antibody. In 2021, 679 sera collected in 2014–2021 were retested retrospectively with three commercial ELISA kits and one batch of an in-house PhI/PhII-ELISA. A low-level shedding of C.burnetii (<10³ mean C.burnetii/swab) was observed until 2014. In 2021 C.burnetii was detected in two animals (<10³.¹C.burnetii/swab), but vaginal swabs collected at two subsequent lambing seasons remained negative. Seroconversion of sentinels was detected until 2017. However, the retrospective analysis of sentinels in 2021 revealed additional single seropositive animals from 2018 to 2021. IFN-γ reactivity was observed during the whole study period; it peaked in 2014 and in 2018 and decreased thereafter. The sporadic detection of C.burnetii and the immune responses of sentinels suggested that a subliminal infection persisted despite vaccination. Nevertheless, vaccination of gimmers prevented the development of a major outbreak, it controlled the infection and reduced the risk of human infection.
Keywords	Coxiella ; Q fever ; bacteria ; flocks ; human diseases ; monoclonal antibodies ; nose ; retrospective studies ; seroconversion ; seroprevalence ; vaccination ; vaccines
Language	English
Dates of publication	2022-0721
Publishing place	Elsevier Ltd
Document type	Article
Note	Pre-press version
ZDB-ID	605674-x
ISSN	1873-2518 ; 0264-410X
ISSN (online)	1873-2518
ISSN	0264-410X
DOI	10.1016/j.vaccine.2022.07.029
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Cologne/Königswinter

Zs.A 1930: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article: Isolation and Cultivation of a New Isolate of BTV-25 and Presumptive Evidence for a Potential Persistent Infection in Healthy Goats

Ries, Christina / Domes, Ursula / Janowetz, Britta / Böttcher, Jens / Burkhardt, Katinka / Miller, Thomas / Beer, Martin / Hoffmann, Bernd

Viruses. 2020 Sept. 04, v. 12, no. 9

2020

Abstract: Recently, several so-called “atypical” Bluetongue virus (BTV) serotypes were discovered, including BTV-25 (Toggenburg virus), in Switzerland. Most “atypical” BTV were identified in small ruminants without clinical signs. In 2018, two goats from a holding ...

Abstract	Recently, several so-called “atypical” Bluetongue virus (BTV) serotypes were discovered, including BTV-25 (Toggenburg virus), in Switzerland. Most “atypical” BTV were identified in small ruminants without clinical signs. In 2018, two goats from a holding in Germany tested positive for BTV-25 genome by RT-qPCR prior to export. After experimental inoculation of the two goats with the BTV-25 positive field blood samples for generation of reference materials, viremia could be observed in one animal. For the first time, the BTV-25-related virus was isolated in cell culture from EDTA-blood and the full genome of isolate “BTV-25-GER2018” could be generated. BTV-25-GER2018 was only incompletely neutralized by ELISA-positive sera. We could monitor the BTV-25 occurrence in the respective affected goat flock of approximately 120 goats over several years. EDTA blood samples were screened with RT-qPCR using a newly developed BTV-25 specific assay. For serological surveillance, serum samples were screened using a commercial cELISA. BTV-25-GER2018 was detected over 4.5 years in the goat flock with intermittent PCR-positivity in some animals, and with or without concomitantly detected antibodies since 2015. We could demonstrate the viral persistence of BTV-25-GER2018 in goats for up to 4.5 years, and the first BTV-25 isolate is now available for further characterization.
Keywords	Bluetongue virus ; EDTA (chelating agent) ; Toggenburg ; antibodies ; blood sampling ; blood serum ; cell culture ; chronic diseases ; enzyme-linked immunosorbent assay ; exports ; flocks ; genome ; goat diseases ; goats ; monitoring ; neutralization ; quantitative polymerase chain reaction ; reverse transcriptase polymerase chain reaction ; serotypes ; signs and symptoms (animals and humans) ; viremia ; viruses ; Germany ; Switzerland
Language	English
Dates of publication	2020-0904
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2516098-9
ISSN	1999-4915
ISSN	1999-4915
DOI	10.3390/v12090983
Database	NAL-Catalogue (AGRICOLA)

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Book ; Online ; Thesis: Untersuchungen über die Bestimmung der Spermaqualität - insbesondere Motilität und Membranintegrität - und Zusammenhänge zur Fertilität von Besamungshengsten

Domes, Ursula

2003

Author's details	von Ursula Domes
Language	German
Size	Online-Ressource
Document type	Book ; Online ; Thesis
Thesis / German Habilitation thesis	Univ., Diss--München, 2003
Database	Former special subject collection: coastal and deep sea fishing

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Article ; Online: Long-term control of Coxiellosis in sheep by annual primary vaccination of gimmers.

Böttcher, Jens / Bauer, Benjamin U / Ambros, Christina / Alex, Michaela / Domes, Ursula / Roth, Sabine / Boll, Kerstin / Korneli, Martin / Bogner, Karl-Heinz / Randt, Andreas / Janowetz, Britta

Vaccine

2022 Volume 40, Issue 35, Page(s) 5197–5206

Abstract	Coxiella (C.) burnetii, a Gram-negative intracellular bacterium, causes Q fever in humans and Coxiellosis in animals. Ruminants are a primary source of human infection with C.burnetii. In 2013, vaccination was implemented in a sheep flock with 650 ewes associated with two outbreaks of Q fever in humans in 2008 and 2012. Only gimmers (yearlings) received two doses of a commercial C.burnetii phase I whole cell vaccine three weeks apart (primary vaccination) without any revaccination. Vaginal and nasal swabs collected shortly after lambing were tested by qPCR. Additionally, a group of non-vaccinated sentinels was serologically monitored for phase I (PhI), II (PhII) antibodies and for Interferon γ (IFN-γ) after stimulation of whole blood cells with PhII-antigen with and without an IL-10-neutralizing monoclonal antibody. In 2021, 679 sera collected in 2014-2021 were retested retrospectively with three commercial ELISA kits and one batch of an in-house PhI/PhII-ELISA. A low-level shedding of C.burnetii (<10
MeSH term(s)	Animals ; Coxiella burnetii ; Female ; Humans ; Q Fever/epidemiology ; Q Fever/prevention & control ; Q Fever/veterinary ; Retrospective Studies ; Sheep ; Sheep Diseases/epidemiology ; Sheep Diseases/microbiology ; Sheep Diseases/prevention & control ; Vaccination/veterinary
Language	English
Publishing date	2022-07-29
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	605674-x
ISSN	1873-2518 ; 0264-410X
ISSN (online)	1873-2518
ISSN	0264-410X
DOI	10.1016/j.vaccine.2022.07.029
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1930: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 458: Show issues

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Article ; Online: Isolation and Cultivation of a New Isolate of BTV-25 and Presumptive Evidence for a Potential Persistent Infection in Healthy Goats.

Ries, Christina / Domes, Ursula / Janowetz, Britta / Böttcher, Jens / Burkhardt, Katinka / Miller, Thomas / Beer, Martin / Hoffmann, Bernd

Viruses

2020 Volume 12, Issue 9

Abstract: Recently, several so-called "atypical" Bluetongue virus (BTV) serotypes were discovered, including BTV-25 (Toggenburg virus), in Switzerland. Most "atypical" BTV were identified in small ruminants without clinical signs. In 2018, two goats from a holding ...

Abstract	Recently, several so-called "atypical" Bluetongue virus (BTV) serotypes were discovered, including BTV-25 (Toggenburg virus), in Switzerland. Most "atypical" BTV were identified in small ruminants without clinical signs. In 2018, two goats from a holding in Germany tested positive for BTV-25 genome by RT-qPCR prior to export. After experimental inoculation of the two goats with the BTV-25 positive field blood samples for generation of reference materials, viremia could be observed in one animal. For the first time, the BTV-25-related virus was isolated in cell culture from EDTA-blood and the full genome of isolate "BTV-25-GER2018" could be generated. BTV-25-GER2018 was only incompletely neutralized by ELISA-positive sera. We could monitor the BTV-25 occurrence in the respective affected goat flock of approximately 120 goats over several years. EDTA blood samples were screened with RT-qPCR using a newly developed BTV-25 specific assay. For serological surveillance, serum samples were screened using a commercial cELISA. BTV-25-GER2018 was detected over 4.5 years in the goat flock with intermittent PCR-positivity in some animals, and with or without concomitantly detected antibodies since 2015. We could demonstrate the viral persistence of BTV-25-GER2018 in goats for up to 4.5 years, and the first BTV-25 isolate is now available for further characterization.
MeSH term(s)	Animals ; Antibodies, Viral/blood ; Blood/virology ; Bluetongue/blood ; Bluetongue/virology ; Bluetongue virus/classification ; Bluetongue virus/genetics ; Bluetongue virus/growth & development ; Bluetongue virus/isolation & purification ; Genome, Viral ; Goat Diseases/blood ; Goat Diseases/virology ; Goats
Chemical Substances	Antibodies, Viral
Language	English
Publishing date	2020-09-04
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v12090983
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Isolation and Cultivation of a New Isolate of BTV-25 and Presumptive Evidence for a Potential Persistent Infection in Healthy Goats

Ries, Christina / Domes, Ursula / Janowetz, Britta / Böttcher, Jens / Burkhardt, Katinka / Miller, Thomas / Beer, Martin / Hoffmann, Bernd

2020

Abstract	Recently, several so-called “atypical” Bluetongue virus (BTV) serotypes were discovered, including BTV-25 (Toggenburg virus), in Switzerland. Most “atypical” BTV were identified in small ruminants without clinical signs. In 2018, two goats from a holding in Germany tested positive for BTV-25 genome by RT-qPCR prior to export. After experimental inoculation of the two goats with the BTV-25 positive field blood samples for generation of reference materials, viremia could be observed in one animal. For the first time, the BTV-25-related virus was isolated in cell culture from EDTA-blood and the full genome of isolate “BTV-25-GER2018” could be generated. BTV-25-GER2018 was only incompletely neutralized by ELISA-positive sera. We could monitor the BTV-25 occurrence in the respective affected goat flock of approximately 120 goats over several years. EDTA blood samples were screened with RT-qPCR using a newly developed BTV-25 specific assay. For serological surveillance, serum samples were screened using a commercial cELISA. BTV-25-GER2018 was detected over 4.5 years in the goat flock with intermittent PCR-positivity in some animals, and with or without concomitantly detected antibodies since 2015. We could demonstrate the viral persistence of BTV-25-GER2018 in goats for up to 4.5 years, and the first BTV-25 isolate is now available for further characterization.
Keywords	Text ; ddc:570 ; Bluetongue virus -- BTV -- atypical BTV -- serotype 25 -- persistent infection -- goats
Subject code	630
Language	English
Publishing date	2020-09-04
Publishing country	de
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Book ; Online ; Thesis: Untersuchungen über die Bestimmung der Spermaqualität - insbesondere Motilität und Membranintegrität - und Zusammenhänge zur Fertilität von Besamungshengsten

Domes, Ursula [Verfasser]

2003

Author's details	von Ursula Domes
Keywords	Landwirtschaft, Veterinärmedizin ; Agriculture, Veterinary Science
Subject code	sg630
Document type	Book ; Online ; Thesis
Database	Digital theses on the web

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Conference proceedings ; Online: Zuchtprogramme ohne Biotechnologie - Etablierung von Bockringen in der Ziegenzüchtung

Herold, Pera / Bürstel, Daniela / Domes, Ursula / Spengler, Dieter / Mendel, Christian / Wenzler, Johann-Georg / Rogg, Rudolf / Hamann, Henning / Valle Zárate, Anne

2013

Abstract: Within organic livestock farming, the use of AI versus natural mating is under steady discussion. However, no special organic breeding programs without AI are set in place so far. The present study takes goat breeding in Germany as an example to show how ...

Abstract	Within organic livestock farming, the use of AI versus natural mating is under steady discussion. However, no special organic breeding programs without AI are set in place so far. The present study takes goat breeding in Germany as an example to show how an organic breeding program without biotechnology could be organised. As basis, breeding planning on different breeding plans is carried out: the breeding program in place, a breeding program with buck rotation within “buck circles” and a breeding program with AI are evaluated. It can be shown that within smaller populations (here: goat breeding in South Germany) a buck rotation scheme with progeny testing is superior in genetic gain than the actual and a breeding program based on AI. In a second step, in discussions with different stakeholders and farmers the hygiene plan of the buck circle was discovered as the critical point. A detailed hygiene plan has to be worked out and agreed upon by all participants. Still, the participating farmers have to trust all members of the group, because the compliance with the regulations is only limited controllable.
Keywords	Breeding and genetics
Subject code	590
Language	German
Publishing date	2013-09-09
Publishing country	dk
Document type	Conference proceedings ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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