Article: Unveiling the mono-rhamnolipid and di-rhamnolipid mechanisms of action upon plasma membrane models
Journal of colloid and interface science. 2022 May 25,
2022
Abstract: Rhamnolipids (RLs) are biosurfactants with significant tensioactive and emulsifying properties. They are mainly composed by mono-RL and di-RL components. Although there are numerous studies concerning their molecular properties, information is scarce ... ...
| Abstract | Rhamnolipids (RLs) are biosurfactants with significant tensioactive and emulsifying properties. They are mainly composed by mono-RL and di-RL components. Although there are numerous studies concerning their molecular properties, information is scarce regarding the mechanisms by which each of the two components interacts with cell membranes. Herein, we performed phase-contrast and fluorescence microscopy experiments on plasma membrane models represented by giant-unilamellar-vesicles (GUVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 2-[[(E,2S,3R)-1,3-dihydroxy-2-(octadecanoylamino)octadec-4-enyl]peroxy-hydroxyphosphoryl]oxyethyl-trimethylazanium (sphingomyelin, SM) and (3β)-cholest-5-en-3-ol (cholesterol, CHOL) (1:1:1 molar ratio), which present liquid-order (Lo) liquid-disorder (Ld) phase coexistence, in the presence of either mono-RL or di-RL in 0.06-0.25 mM concentration range. A new method has been developed to determine area and volume of GUVs with asymmetrical shape and a kinetic model describing GUV-RL interaction in terms of two mechanisms, RL-insertion and pore formation, has been worked out. Results show that the insertion of mono-RL in the membrane outer leaflet is the dominant process with no pore formation and a negligible effect in modifying membrane permeability, but induces lipid mixing. Conversely, the di-RL-GUV interaction begins with the insertion mechanism and, as the time passes by, the pore formation process occurs. The analyses of di-RL show that the whole process is only relevant in the Ld phase with a higher extent to 0.25 mM than to 0.06 mM. |
|---|---|
| Keywords | biosurfactants ; cholesterol ; fluorescence microscopy ; kinetics ; membrane permeability ; plasma membrane ; rhamnolipids ; sphingomyelins |
| Language | English |
| Dates of publication | 2022-0525 |
| Publishing place | Elsevier Inc. |
| Document type | Article |
| Note | Pre-press version |
| ZDB-ID | 241597-5 |
| ISSN | 1095-7103 ; 0021-9797 |
| ISSN (online) | 1095-7103 |
| ISSN | 0021-9797 |
| DOI | 10.1016/j.jcis.2022.05.145 |
| Database | NAL-Catalogue (AGRICOLA) |
Full text online
More links
Kategorien
In stock of ZB MED Bonn / Germany
| Z 71/707: Show issues |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.
Inter-library loan at ZB MED
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.