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Article ; Online: Liquid-liquid phase separation in plants: Advances and perspectives from model species to crops.

Liu, Qianwen / Liu, Wenxuan / Niu, Yiding / Wang, Tao / Dong, Jiangli

Plant communications

2023 Volume 5, Issue 1, Page(s) 100663

Abstract: Membraneless biomolecular condensates play important roles in both normal biological activities and responses to environmental stimuli in living organisms. Liquid‒liquid phase separation (LLPS) is an organizational mechanism that has emerged in recent ... ...

Abstract	Membraneless biomolecular condensates play important roles in both normal biological activities and responses to environmental stimuli in living organisms. Liquid‒liquid phase separation (LLPS) is an organizational mechanism that has emerged in recent years to explain the formation of biomolecular condensates. In the past decade, advances in LLPS research have contributed to breakthroughs in disease fields. By contrast, although LLPS research in plants has progressed over the past 5 years, it has been concentrated on the model plant Arabidopsis, which has limited relevance to agricultural production. In this review, we provide an overview of recently reported advances in LLPS in plants, with a particular focus on photomorphogenesis, flowering, and abiotic and biotic stress responses. We propose that many potential LLPS proteins also exist in crops and may affect crop growth, development, and stress resistance. This possibility presents a great challenge as well as an opportunity for rigorous scientific research on the biological functions and applications of LLPS in crops.
MeSH term(s)	Intrinsically Disordered Proteins/metabolism ; Phase Separation
Chemical Substances	Intrinsically Disordered Proteins
Language	English
Publishing date	2023-07-26
Publishing country	China
Document type	Journal Article ; Review
ISSN	2590-3462
ISSN (online)	2590-3462
DOI	10.1016/j.xplc.2023.100663
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Article ; Online: Autophagy in Plant Abiotic Stress Management.

Chen, Hong / Dong, Jiangli / Wang, Tao

International journal of molecular sciences

2021 Volume 22, Issue 8

Abstract: Plants can be considered an open system. Throughout their life cycle, plants need to exchange material, energy and information with the outside world. To improve their survival and complete their life cycle, plants have developed sophisticated mechanisms ...

Abstract	Plants can be considered an open system. Throughout their life cycle, plants need to exchange material, energy and information with the outside world. To improve their survival and complete their life cycle, plants have developed sophisticated mechanisms to maintain cellular homeostasis during development and in response to environmental changes. Autophagy is an evolutionarily conserved self-degradative process that occurs ubiquitously in all eukaryotic cells and plays many physiological roles in maintaining cellular homeostasis. In recent years, an increasing number of studies have shown that autophagy can be induced not only by starvation but also as a cellular response to various abiotic stresses, including oxidative, salt, drought, cold and heat stresses. This review focuses mainly on the role of autophagy in plant abiotic stress management.
MeSH term(s)	Autophagy ; Models, Biological ; Plants/metabolism ; Saccharomyces cerevisiae/cytology ; Saccharomyces cerevisiae/metabolism ; Stress, Physiological
Language	English
Publishing date	2021-04-15
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms22084075
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Article ; Online: The S-acylation cycle of transcription factor MtNAC80 influences cold stress responses in Medicago truncatula.

Ye, Qinyi / Zheng, Lihua / Liu, Peng / Liu, Qianwen / Ji, Tuo / Liu, Jinling / Gao, Yajuan / Liu, Li / Dong, Jiangli / Wang, Tao

The Plant cell

2024

Abstract: S-acylation is a reversible post-translational modification catalyzed by protein S-acyltransferases (PATs), and acyl protein thioesterases (APTs) mediate de-S-acylation. Although many proteins are S-acylated, how the S-acylation cycle modulates specific ... ...

Abstract	S-acylation is a reversible post-translational modification catalyzed by protein S-acyltransferases (PATs), and acyl protein thioesterases (APTs) mediate de-S-acylation. Although many proteins are S-acylated, how the S-acylation cycle modulates specific biological functions in plants is poorly understood. In this study, we report that the S-acylation cycle of transcription factor MtNAC80 is involved in the Medicago truncatula cold stress response. Under normal conditions, MtNAC80 localized to membranes through MtPAT9-induced S-acylation. In contrast, under cold stress conditions, MtNAC80 translocated to the nucleus through de-S-acylation mediated by thioesterases such as MtAPT1. MtNAC80 functions in the nucleus by directly binding the promoter of the glutathione S-transferase gene MtGSTU1 and promoting its expression, which enables plants to survive under cold stress by removing excess malondialdehyde and H2O2. Our findings reveal an important function of the S-acylation cycle in plants and provide insight into stress response and tolerance mechanisms.
Language	English
Publishing date	2024-03-29
Publishing country	England
Document type	Journal Article
ZDB-ID	623171-8
ISSN	1532-298X ; 1040-4651
ISSN (online)	1532-298X
ISSN	1040-4651
DOI	10.1093/plcell/koae103
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Article ; Online: Multigene editing reveals that MtCEP1/2/12 redundantly control lateral root and nodule number in Medicago truncatula.

Zhu, Fugui / Ye, Qinyi / Chen, Hong / Dong, Jiangli / Wang, Tao

Journal of experimental botany

2021 Volume 72, Issue 10, Page(s) 3661–3676

Abstract: The multimember CEP (C-terminally Encoded Peptide) gene family is a complex group that is involved in various physiological activities in plants. Previous studies demonstrated that MtCEP1 and MtCEP7 control lateral root formation or nodulation, but these ...

Abstract	The multimember CEP (C-terminally Encoded Peptide) gene family is a complex group that is involved in various physiological activities in plants. Previous studies demonstrated that MtCEP1 and MtCEP7 control lateral root formation or nodulation, but these studies were based only on gain of function or artificial miRNA (amiRNA)/RNAi approaches, never knockout mutants. Moreover, an efficient multigene editing toolkit is not currently available for Medicago truncatula. Our quantitative reverse transcription-PCR data showed that MtCEP1, 2, 4, 5, 6, 7, 8, 9, 12, and 13 were up-regulated under nitrogen starvation conditions and that MtCEP1, 2, 7, 9, and 12 were induced by rhizobial inoculation. Treatment with synthetic MtCEP peptides of MtCEP1, 2, 4, 5, 6, 8, and 12 repressed lateral root emergence and promoted nodulation in the R108 wild type but not in the cra2 mutant. We optimized CRISPR/Cas9 [clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9] genome editing system for M. truncatula, and thus created single mutants of MtCEP1, 2, 4, 6, and 12 and the double mutants Mtcep1/2C and Mtcep5/8C; however, these mutants did not exhibit significant differences from R108. Furthermore, a triple mutant Mtcep1/2/12C and a quintuple mutant Mtcep1/2/5/8/12C were generated and exhibited more lateral roots and fewer nodules than R108. Overall, MtCEP1, 2, and 12 were confirmed to be redundantly important in the control of lateral root number and nodulation. Moreover, the CRISPR/Cas9-based multigene editing protocol provides an additional tool for research on the model legume M. truncatula, which is highly efficient at multigene mutant generation.
MeSH term(s)	Gene Editing ; Medicago truncatula/genetics ; Plant Proteins/genetics ; Plant Root Nodulation/genetics ; Plant Roots/genetics ; Rhizobium ; Root Nodules, Plant/genetics ; Symbiosis
Chemical Substances	Plant Proteins
Language	English
Publishing date	2021-02-23
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2976-2
ISSN	1460-2431 ; 0022-0957
ISSN (online)	1460-2431
ISSN	0022-0957
DOI	10.1093/jxb/erab093
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Article ; Online: All-optical light manipulation based on graphene-embedded side-polished fiber.

Huang, Quandong / Zhong, Lixi / Dong, Jiangli / Xu, Ou / Zheng, Zhaoqiang / Huang, Tianxiong / Li, Jianping / Xiang, Meng / Fu, Songnian / Qin, Yuwen

Optics letters

2022 Volume 47, Issue 6, Page(s) 1478–1481

Abstract: We present a study of all-optical light manipulation arising in a graphene-embedded side-polished fiber (SPF) with a Norland Optical Adhesives (NOA)-coated structure. With the help of the Pauli blocking effect, such an all-fiber device serves to manage ... ...

Abstract	We present a study of all-optical light manipulation arising in a graphene-embedded side-polished fiber (SPF) with a Norland Optical Adhesives (NOA)-coated structure. With the help of the Pauli blocking effect, such an all-fiber device serves to manage the loss of transverse-electric-polarized light when the control light and the signal light are polarized along the direction parallel to the graphene surface. The insertion loss of this device can be effectively reduced with the NOA coating. An enhanced interaction between the graphene and the propagated light can be achieved via the strong evanescent field of the SPF and longer interaction length. This results in effective all-optical manipulation of light with a modulation depth of 10.4 dB (or modulation efficiency of ∼91%) and a modulation slope of ∼1.3, where the required control power is only about 14 dBm. The device has broadband operation wavelength. The insertion loss for both the signal light and the control light are only about 0.6 dB. The experimental results are well-fitting with the simulation study. Such an all-fiber device has the potential for all-optical signal processing.
Language	English
Publishing date	2022-03-15
Publishing country	United States
Document type	Journal Article
ISSN	1539-4794
ISSN (online)	1539-4794
DOI	10.1364/OL.452612
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Article ; Online: Ultra-broadband LP

Huang, Quandong / Wang, Xibin / Dong, Jiangli / Zheng, Zhaoqiang / Xu, Ou / Fu, Songnian / Peng, Di / Li, Jianping / Qin, Yuwen

Optics express

2022 Volume 30, Issue 8, Page(s) 12751–12759

Abstract: We report an ultra-broadband ... ...

Abstract	We report an ultra-broadband LP
Language	English
Publishing date	2022-04-26
Publishing country	United States
Document type	Journal Article
ZDB-ID	1491859-6
ISSN	1094-4087 ; 1094-4087
ISSN (online)	1094-4087
ISSN	1094-4087
DOI	10.1364/OE.454537
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Article ; Online: Dynamic Protein S-Acylation in Plants.

Zheng, Lihua / Liu, Peng / Liu, Qianwen / Wang, Tao / Dong, Jiangli

International journal of molecular sciences

2019 Volume 20, Issue 3

Abstract: Lipid modification is an important post-translational modification. S-acylation is unique among lipid modifications, as it is reversible and has thus attracted much attention. We summarize some proteins that have been shown experimentally to be S- ... ...

Abstract	Lipid modification is an important post-translational modification. S-acylation is unique among lipid modifications, as it is reversible and has thus attracted much attention. We summarize some proteins that have been shown experimentally to be S-acylated in plants. Two of these S-acylated proteins have been matched to the S-acyl transferase. More importantly, the first protein thioesterase with de-S-acylation activity has been identified in plants. This review shows that S-acylation is important for a variety of different functions in plants and that there are many unexplored aspects of S-acylation in plants.
MeSH term(s)	Acylation/physiology ; Lipid Metabolism/physiology ; Models, Biological ; Monomeric GTP-Binding Proteins/metabolism ; Plant Proteins/metabolism ; Protein Processing, Post-Translational ; Transcription Factors/metabolism
Chemical Substances	Plant Proteins ; Transcription Factors ; Monomeric GTP-Binding Proteins (EC 3.6.5.2)
Language	English
Publishing date	2019-01-29
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms20030560
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Article: From model to alfalfa: Gene editing to obtain semidwarf and prostrate growth habits

Zheng, Lihua / Wen, Jiangqi / Liu, Jinling / Meng, Xiangzhao / Liu, Peng / Cao, Na / Dong, Jiangli / Wang, Tao

Crop Science Society of China and Institute of Crop Science, CAAS crop journal. 2021 Nov. 29,

2021

Abstract: Alfalfa (Medicago sativa L.) is a nutritious forage crop with wide ecological adaptability. The molecular breeding of alfalfa is restricted by its heterozygous tetraploid genome and the difficult genetic manipulation process. Under time and resource ... ...

Abstract	Alfalfa (Medicago sativa L.) is a nutritious forage crop with wide ecological adaptability. The molecular breeding of alfalfa is restricted by its heterozygous tetraploid genome and the difficult genetic manipulation process. Under time and resource constraints, we applied a more convenient approach. We investigated two MtGA3ox genes, MtGA3ox1 and MtGA3ox2, of Medicago truncatula, a diploid legume model species, finding that MtGA3ox1 plays a major role in GA-regulated plant architecture. Mutation of neither gene affected nitrogenase activity. These results suggest that MtGA3ox1 can be used in semidwarf and prostrate alfalfa breeding. Based on the M. truncatula MtGA3ox1 sequence, MsGA3ox1 was cloned from alfalfa, and two knockout targets were designed. An efficient CRISPR/Cas9-based genome editing protocol was used to generate msga3ox1 mutants in alfalfa. We obtained three lines that carried mutations in all four alleles in the T0 generation. Fifteen clonal plants were vegetatively propagated from each transgenic line using shoot cuttings. The plant height and internode length of msga3ox1 null mutants were significantly decreased. The number of total lateral branches, leaf/stem ratio and crude protein content of aerial plant parts of msga3ox1 mutants were significantly increased. Thus, we obtained semi-dwarf and prostrate alfalfa by gene editing.
Keywords	Medicago sativa ; Medicago truncatula ; alfalfa ; crude protein ; diploidy ; forage crops ; genetically modified organisms ; heterozygosity ; internode length ; leaves ; models ; mutation ; nitrogenase ; plant architecture ; plant height ; tetraploidy
Language	English
Dates of publication	2021-1129
Publishing place	Elsevier B.V.
Document type	Article
Note	Pre-press version
ZDB-ID	2745450-2
ISSN	2214-5141
ISSN	2214-5141
DOI	10.1016/j.cj.2021.11.008
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Article: Histone Methyltransferase SUVR2 Promotes the DSB Repair via Chromatin Remodeling and Liquid–Liquid Phase Separation

Liu, Qianwen / Liu, Peng / Ji, Tuo / Zheng, Lihua / Shen, Chen / Ran, Shasha / Liu, Jinling / Zhao, Yafei / Niu, Yiding / Wang, Tao / Dong, Jiangli

Molecular plant. 2022 May 18,

2022

Abstract: Maintaining genomic integrity and stability is particularly important for stem cells, which are at the top of the cell lineage origin. Here, we discovered that the plant-specific histone methyltransferase SUVR2 maintains the integrity of the root tip ... ...

Abstract	Maintaining genomic integrity and stability is particularly important for stem cells, which are at the top of the cell lineage origin. Here, we discovered that the plant-specific histone methyltransferase SUVR2 maintains the integrity of the root tip stem cell genome through chromatin remodeling and liquid–liquid phase separation (LLPS) when facing DNA double-strand breaks (DSBs). MtSUVR2 has histone methyltransferase activity and catalyzes the conversion of H3K9me1 to H3K9me2/3 in vitro and in Medicago truncatula. Under DNA damage, the proportion of heterochromatin decreased, and the DSB damage marker γ-H2AX level increased in the suvr2 mutants, indicating that MtSUVR2 compacted the chromatin structure through H3K9 methylation modification to protect DNA from damage. More importantly, MtSUVR2 was induced by DSBs to phase separate and form droplets to localize at the damage sites, which was confirmed by immunofluorescence and fluorescence recovery after photobleaching (FRAP) experiments. The IDR1 and LCD regions of MtSUVR2 determined the phase separation in nuclei, while the IDR2 region determined the interaction with homologous recombinase MtRAD51. MtSUVR2 drove the phase separation of MtRAD51 to form “DNA repair bodies”, which enhanced the stability of MtRAD51 proteins to facilitate the error-free homologous recombination (HR) repair of stem cells. This study reveals that chromatin remodeling-associated proteins participate in DNA repair through LLPS.
Keywords	DNA ; DNA damage ; DNA repair ; Medicago truncatula ; fluorescence recovery after photobleaching ; fluorescent antibody technique ; genome ; genomics ; heterochromatin ; histones ; homologous recombination ; methylation ; methyltransferases ; recombinases ; root tips ; separation ; stem cells
Language	English
Dates of publication	2022-0518
Publishing place	Elsevier Inc.
Document type	Article
Note	Pre-press version
ZDB-ID	2393618-6
ISSN	1752-9867 ; 1674-2052
ISSN (online)	1752-9867
ISSN	1674-2052
DOI	10.1016/j.molp.2022.05.007
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Article ; Online: The thioesterase APT1 is a bidirectional-adjustment redox sensor.

Ji, Tuo / Zheng, Lihua / Wu, Jiale / Duan, Mei / Liu, Qianwen / Liu, Peng / Shen, Chen / Liu, Jinling / Ye, Qinyi / Wen, Jiangqi / Dong, Jiangli / Wang, Tao

Nature communications

2023 Volume 14, Issue 1, Page(s) 2807

Abstract: The adjustment of cellular redox homeostasis is essential in when responding to environmental perturbations, and the mechanism by which cells distinguish between normal and oxidized states through sensors is also important. In this study, we found that ... ...

Abstract	The adjustment of cellular redox homeostasis is essential in when responding to environmental perturbations, and the mechanism by which cells distinguish between normal and oxidized states through sensors is also important. In this study, we found that acyl-protein thioesterase 1 (APT1) is a redox sensor. Under normal physiological conditions, APT1 exists as a monomer through S-glutathionylation at C20, C22 and C37, which inhibits its enzymatic activity. Under oxidative conditions, APT1 senses the oxidative signal and is tetramerized, which makes it functional. Tetrameric APT1 depalmitoylates S-acetylated NAC (NACsa), and NACsa relocates to the nucleus, increases the cellular glutathione/oxidized glutathione (GSH/GSSG) ratio through the upregulation of glyoxalase I expression, and resists oxidative stress. When oxidative stress is alleviated, APT1 is found in monomeric form. Here, we describe a mechanism through which APT1 mediates a fine-tuned and balanced intracellular redox system in plant defence responses to biotic and abiotic stresses and provide insights into the design of stress-resistant crops.
MeSH term(s)	Cell Nucleus/metabolism ; Glutathione/metabolism ; Glutathione Disulfide/metabolism ; Lactoylglutathione Lyase/metabolism ; Medicago truncatula/genetics ; Medicago truncatula/metabolism ; Oxidation-Reduction ; Oxidative Stress ; Thiolester Hydrolases
Chemical Substances	Glutathione (GAN16C9B8O) ; Glutathione Disulfide (ULW86O013H) ; Lactoylglutathione Lyase (EC 4.4.1.5) ; Thiolester Hydrolases (EC 3.1.2.-)
Language	English
Publishing date	2023-05-17
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-023-38464-y
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