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Article ; Online: In Situ Quantitative Imaging of Plasma Membrane Stiffness in Live Cells Using a Genetically Encoded FRET Sensor.

Hu, Yusi / Wen, Hai-Yan / Liu, Meng-Yao / Wang, Juan-Mei / Dong, Ruo-Lan / Liu, Shu-Lin / Wang, Zhi-Gang

2024

Abstract: Cell membrane stiffness is critical for cellular function, with cholesterol and sphingomyelin as pivot contributors. Current methods for measuring membrane stiffness are often invasive, ex situ, and slow in process, prompting the need for innovative ... ...

Abstract	Cell membrane stiffness is critical for cellular function, with cholesterol and sphingomyelin as pivot contributors. Current methods for measuring membrane stiffness are often invasive, ex situ, and slow in process, prompting the need for innovative techniques. Here, we present a fluorescence resonance energy transfer (FRET)-based protein sensor designed to address these challenges. The sensor consists of two fluorescent units targeting sphingomyelin and cholesterol, connected by a linker that responds to the proximity of these lipids. In rigid membranes, cholesterol and sphingomyelin are in close proximity, leading to an increased FRET signal. We utilized this sensor in combination with confocal microscopy to explore changes in plasma membrane stiffness under various conditions, including differences in osmotic pressure, the presence of reactive oxygen species (ROS) and variations in substrate stiffness. Furthermore, we explored the impact of SARS-CoV-2 on membrane stiffness and the distribution of ACE2 after attachment to the cell membrane. This tool offers substantial potential for future investigations in the field of mechanobiology.
Language	English
Publishing date	2024-05-08
Publishing country	United States
Document type	Journal Article
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.4c00433
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Article: 11,12-EET suppressed LPS induced TF expression and thrombus formation by accelerating mRNA degradation rate via strengthening PI3K-Akt signaling pathway and inhibiting p38-TTP pathway.

Luo, Liman / Yang, Yan / Fu, Menglu / Luo, Jinlan / Li, Wenhua / Tu, Ling / Dong, Ruolan

Prostaglandins & other lipid mediators

2023 Volume 167, Page(s) 106740

Abstract: Epoxyeicosatrienoic acids (EETs), which are synthesized from arachidonic acid by cytochrome P450 epoxygenases, function primarily as autocrine and paracrine effectors in the cardiovascular system. So far, most research has focused on the vasodilatory, ... ...

Abstract	Epoxyeicosatrienoic acids (EETs), which are synthesized from arachidonic acid by cytochrome P450 epoxygenases, function primarily as autocrine and paracrine effectors in the cardiovascular system. So far, most research has focused on the vasodilatory, anti-inflammatory, anti-apoptotic and mitogenic properties of EETs in the systemic circulation. However, whether EETs could suppress tissue factor (TF) expression and prevent thrombus formation remains unknown. Here we utilized in vivo and in vitro models to investigate the effects and underlying mechanisms of exogenously EETs on LPS induced TF expression and inferior vein cava ligation induced thrombosis. We observed that the thrombus formation rate and the size of the thrombus were greatly reduced in 11,12-EET treated mice，accompanied by decreased TF and inflammatory cytokines expression. Further in vitro studies showed that by enhancing p38 MAPK activation and subsequent tristetraprolin (TTP) phosphorylation, LPS strengthened the stability of TF mRNA and induced increased TF expression. However, by strengthening PI3K-dependent Akt phosphorylation, which acted as a negative regulator of p38-TTP signaling pathway，11,12-EET reduced LPS-induced TF expression in monocytes. In addition, 11,12-EET inhibited LPS-induced NF-κB nuclear translocation by activating the PI3K/Akt pathway. Further study indicated that the inhibitory effect of 11,12-EET on TF expression was mediated by antagonizing LPS-induced activation of thromboxane prostanoid receptor. In conclusion, our study demonstrated that 11,12-EET prevented thrombosis by reducing TF expression and targeting the CYP2J2 epoxygenase pathway may represent a novel approach to mitigate thrombosis related diseases.
MeSH term(s)	Animals ; Mice ; Proto-Oncogene Proteins c-akt/metabolism ; Lipopolysaccharides/pharmacology ; Thromboplastin/pharmacology ; Phosphatidylinositol 3-Kinases/metabolism ; Cytochrome P-450 Enzyme System/metabolism ; Signal Transduction ; Cytochrome P-450 CYP2J2 ; 8,11,14-Eicosatrienoic Acid/metabolism ; Thrombosis/drug therapy ; RNA Stability
Chemical Substances	Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; Lipopolysaccharides ; Thromboplastin (9035-58-9) ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Cytochrome P-450 Enzyme System (9035-51-2) ; 11,12-epoxy-5,8,14-eicosatrienoic acid (5DOQ38R4UW) ; Cytochrome P-450 CYP2J2 (EC 1.14.14.1) ; 8,11,14-Eicosatrienoic Acid (FC398RK06S)
Language	English
Publishing date	2023-04-28
Publishing country	United States
Document type	Journal Article
ZDB-ID	1426962-4
ISSN	2212-196X ; 1098-8823 ; 0090-6980
ISSN (online)	2212-196X
ISSN	1098-8823 ; 0090-6980
DOI	10.1016/j.prostaglandins.2023.106740
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Article ; Online: Comprehensive Analysis of Aberrantly Expressed Profiles of lncRNAs and miRNAs with Associated ceRNA Network in Lung Adenocarcinoma and Lung Squamous Cell Carcinoma.

Dong, Ruolan / Liu, Jiawei / Sun, Wei / Ping, Wei

Pathology oncology research : POR

2020 Volume 26, Issue 3, Page(s) 1935–1945

Abstract: Lung cancer (LC) continues to be the leading cause of cancer-related deaths worldwide and the prognosis remains poor worldwide. At present, the long non-coding RNAs (lncRNAs) was considered as a part of competing endogenous RNA (ceRNA) network act as ... ...

Abstract	Lung cancer (LC) continues to be the leading cause of cancer-related deaths worldwide and the prognosis remains poor worldwide. At present, the long non-coding RNAs (lncRNAs) was considered as a part of competing endogenous RNA (ceRNA) network act as natural microRNA (miRNA) sponges to regulate protein-coding gene expression. However, functional roles of lncRNA-mediated ceRNAs in LC are insufficiently understood. To classify the specific mechanism of lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC), we comprehensively compared the expression profiles of mRNAs, lncRNAs and miRNAs obtained from 509 LUAD, 473 LUSC tissues and 49 adjacent non-cancerous lung tissues, based on The Cancer Genome Atlas (TCGA). After screening for differently expressed (DE) mRNAs, DEmiRNAs, DElncRNAs and weighted gene co-expression network analysis (WGCNA) (\|log2FC\| > 2.0 and an adjusted p value <0.05), a total of 4478 DEmRNAs, 526 DElncRNAs and 75 DEmiRNAs in LUAD, while 6237 DEmRNAs, 843 DElncRNAs and 117 DEmiRNAs in LUSC were discovered. Interaction (PPI) network analysis was performed to identify 656 nodes and 2987 edges (minimum required interaction score > 0.9), as well as 8 different protein-protein interactions. Gene ontology (GO) analysis mainly associated with cell proliferation. KEGG pathway enrichment analyses most partly associated with metabolism pathway and cytokine-cytokine receptor interaction. Finally, the dysregulated lncRNA-miRNA-ceRNA network was constructed based on correlation analyses and a total of 62 dysregulated lncRNAs, 28 DEmRNAs and 18 DEmiRNAs were involved. The most significant lncRNAs included DElncRNAs, LINC00641 and AC004947.2, miRNAs included miR-6860, miR-1285-3p, miR-767-3p and miR-7974, mRNAs included MAP3K3, FGD3 and ATP1B2. Then we analyzed and described the potential characteristics of biological function and pathological roles of the LUAD and LUSC ceRNA co-regulatory network. Our findings revealed ceRNA network will be beneficial for promoting the understanding of lncRNA-mediated ceRNA regulatory mechanisms in the pathogenesis of LUAD and LUSC.
MeSH term(s)	Adenocarcinoma of Lung/genetics ; Aged ; Biomarkers, Tumor/genetics ; Carcinoma, Squamous Cell/genetics ; Female ; Gene Expression Regulation, Neoplastic/genetics ; Gene Regulatory Networks/genetics ; Humans ; Lung Neoplasms/genetics ; Male ; MicroRNAs/genetics ; Middle Aged ; RNA, Long Noncoding/genetics ; Transcriptome
Chemical Substances	Biomarkers, Tumor ; MicroRNAs ; RNA, Long Noncoding
Language	English
Publishing date	2020-01-02
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1375979-6
ISSN	1532-2807 ; 1219-4956
ISSN (online)	1532-2807
ISSN	1219-4956
DOI	10.1007/s12253-019-00780-4
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Zs.A 4936: Show issues

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Article: Review on melanosis coli and anthraquinone-containing traditional Chinese herbs that cause melanosis coli.

Zhang, Ruiyuan / Huang, Cai / Wu, Fan / Fang, Ke / Jiang, Shujun / Zhao, Yan / Chen, Guang / Dong, Ruolan

Frontiers in pharmacology

2023 Volume 14, Page(s) 1160480

Abstract: Backgrounds: ...

Abstract	Backgrounds:
Language	English
Publishing date	2023-05-04
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2023.1160480
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Article ; Online: HuanglianGanjiang Tang alleviates DSS-induced colitis in mice by inhibiting necroptosis through vitamin D receptor.

Xiong, Xinyu / Cheng, Zhe / Zhou, Yi / Wu, Fan / Xie, Linglin / Lawless, Lauren / Dong, Ruolan / Zhao, Yan / Yu, Lingling / Chen, Guang

Journal of ethnopharmacology

2022 Volume 298, Page(s) 115655

Abstract: Ethnopharmacological relevance: HuanglianGanjiang Tang (HGT) is a classic prescription of traditional Chinese medicine (TCM) recorded in Dan Xi Xin Fa, which was used to alleviate manifestations like diarrhea, abdominal pain and hemafecia. In current ... ...

Abstract	Ethnopharmacological relevance: HuanglianGanjiang Tang (HGT) is a classic prescription of traditional Chinese medicine (TCM) recorded in Dan Xi Xin Fa, which was used to alleviate manifestations like diarrhea, abdominal pain and hemafecia. In current clinical practices, HGT is adopted for the treatment of ulcerative colitis (UC) and affords good curative effect. However, the underlying mechanism deserves further elucidation. Aim of the study: UC is a hard-to-curable and easy-to-recurrent inflammatory disease. This study is to evaluate the potential therapeutics and explore the molecular mechanism of HGT on UC in the mouse model. Materials and methods: The components of HGT extracts were identified by HPLC. The colitis of mice was induced by 3% (w./v.) dextran sulfate sodium (DSS). The HGT decoction was prepared through boiling and centrifuging. The mice were given HGT decoction via oral gavage (0.34 g/ml & 0.68 g/ml; 5 ml/kg b.w.). The protective role of HGT on colitis mice was evaluated by body weight change, colon length, disease activity index (DAI) and histological scores. The expressions of necroptosis-related and vitamin D receptor (VDR)-related proteins were measured by Western blot, RT-qPCR and immunofluorescence. Results: HGT could significantly reduce the loss of body weight and colon length in colitis mice, and alleviated the DAI and histological scores. Mechanically, HGT also promoted the expression of E-cadherin, Occludin, ZO-1 and VDR, and reduced the level of intestinal inflammatory cytokines, such as, IL-6, IL-1β and TNF-α. Besides, HGT downregulated the protein level of p-RIPK3, p-RIPK1 and p-MLKL while upregulated the protein level of Caspase-8 in colon tissue compared to the model group. Conclusion: Our study addressed that HGT can alleviate DSS-induced colitis of mice through inhibiting colonic necroptosis by upregulating the level of VDR.
MeSH term(s)	Animals ; Body Weight ; Colitis/chemically induced ; Colitis/drug therapy ; Colitis/metabolism ; Colitis, Ulcerative/chemically induced ; Colitis, Ulcerative/drug therapy ; Colitis, Ulcerative/pathology ; Colon ; Dextran Sulfate ; Disease Models, Animal ; Mice ; Mice, Inbred C57BL ; Necroptosis ; Receptors, Calcitriol/metabolism ; Receptors, Calcitriol/therapeutic use
Chemical Substances	Receptors, Calcitriol ; Dextran Sulfate (9042-14-2)
Language	English
Publishing date	2022-08-19
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2022.115655
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Article ; Online: Network Pharmacology Research Indicates that Wu-Mei-Wan Treats Obesity by Inhibiting Th17 Cell Differentiation and Alleviating Metabolic Inflammation.

Cheng, Zhe / Xiong, Xinyu / Wu, Fan / Zhao, Yan / Dong, Ruolan / Jiang, Shujun / Fang, Ke / Huang, Panpan / Chen, Guang

Combinatorial chemistry & high throughput screening

2022 Volume 26, Issue 1, Page(s) 30–48

Abstract: Background: Wu-Mei-Wan (WMW), a traditional Chinese medicine (TCM) formula, has a good effect on the treatment of obesity and has been proven helpful to promote the metabolism of adipose tissue. However, its underlying mechanism remains to be studied. ... ...

Abstract	Background: Wu-Mei-Wan (WMW), a traditional Chinese medicine (TCM) formula, has a good effect on the treatment of obesity and has been proven helpful to promote the metabolism of adipose tissue. However, its underlying mechanism remains to be studied. This study aims to explore the potential pharmacological mechanism of WMW in the treatment of obesity. Methods: Network pharmacology was used to sort out the relationship between WMW putative targets and obesity-related drug targets or disease targets, which indicated the mechanism of WMW in treating obesity from two aspects of clinical drugs approved by the Food and Drug Administration (FDA) and obesity-related diseases. Databases such as Traditional Chinese Medicine Systems Pharmacology (TCMSP), PubChem, DrugBank, DisGeNET, and Genecards were used to collect information about targets. String platform was used to convert the data into gene symbol of "homo sapiens", and perform gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. With the Human Protein Reference Database (HPRD) as background data, Cytoscape 3.6.0 software was used to construct a new protein-protein interaction (PPI) network. Mechanism diagrams of key pathways were obtained from the KEGG database. AutoDock Vina software was used to conduct molecular docking verification. Results: The number of targets in the overlap between WMW putative targets and obesity-related drug targets accounted for more than 50% of the latter, and HTR3A, SLC6A4, and CYP3A4 were core targets. In obesity-related disease targets-WMW putative targets PPI network, the Th17 cell differentiation pathway, and the IL-17 signaling pathway were key pathways, and the 1st module and the 7th module were central function modules that were highly associated with immunity and inflammation. Molecular docking verified that STAT3, TGFB1, MMP9, AHR, IL1B, and CCL2 were core targets in the treatment of WMW on obesity. Conclusion: WMW has similar effects on lipid and drug metabolism as the current obesity-related drugs, and is likely to treat obesity by inhibiting Th17 cell differentiation and alleviating metabolic inflammation.
MeSH term(s)	United States ; Humans ; Network Pharmacology ; Molecular Docking Simulation ; Signal Transduction ; Cell Differentiation ; Databases, Protein ; Serotonin Plasma Membrane Transport Proteins
Chemical Substances	SLC6A4 protein, human ; Serotonin Plasma Membrane Transport Proteins
Language	English
Publishing date	2022-02-16
Publishing country	United Arab Emirates
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2064785-2
ISSN	1875-5402 ; 1386-2073
ISSN (online)	1875-5402
ISSN	1386-2073
DOI	10.2174/1386207325666220221121919
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Article: HuanglianGanjiang Tang alleviates DSS-induced colitis in mice by inhibiting necroptosis through vitamin D receptor

Xiong, Xinyu / Cheng, Zhe / Zhou, Yi / Wu, Fan / Xie, Linglin / Lawless, Lauren / Dong, Ruolan / Zhao, Yan / Yu, Lingling / Chen, Guang

Journal of ethnopharmacology. 2022 Aug. 16,

2022

Abstract: HuanglianGanjiang Tang (HGT) is a classic prescription of traditional Chinese medicine (TCM) recorded in Dan Xi Xin Fa, which was used to alleviate manifestations like diarrhea, abdominal pain and hemafecia. In current clinical practices, HGT is adopted ... ...

Abstract	HuanglianGanjiang Tang (HGT) is a classic prescription of traditional Chinese medicine (TCM) recorded in Dan Xi Xin Fa, which was used to alleviate manifestations like diarrhea, abdominal pain and hemafecia. In current clinical practices, HGT is adopted for the treatment of ulcerative colitis (UC) and affords good curative effect. However, the underlying mechanism deserves further elucidation. UC is a hard-to-curable and easy-to-recurrent inflammatory disease. This study is to evaluate the potential therapeutics and explore the molecular mechanism of HGT on UC in the mouse model. The components of HGT extracts were identified by HPLC. The colitis of mice was induced by 3% (w./v.) dextran sulfate sodium (DSS). The HGT decoction was prepared through boiling and centrifuging. The mice were given HGT decoction via oral gavage (0.34g/ml & 0.68g/ml; 5ml/kg b.w.). The protective role of HGT on colitis mice was evaluated by body weight change, colon length, disease activity index (DAI) and histological scores. The expression of necroptosis-related and vitamin D receptor (VDR)-related proteins was measured by western blot, RT-qPCR and immunofluorescence. HGT could significantly reduce the loss of body weight and colon length in colitis mice, and alleviated the DAI and histological score. Mechanically, HGT also promoted the expression of E-cadherin, Occludin, ZO-1 and VDR, and reduced the level of intestinal inflammatory cytokines, such as, IL-6, IL-1β and TNF-α. Besides, HGT downregulated the protein level of p-RIPK3, p-RIPK1 and p-MLKL while upregulated the protein level of Caspase-8 in colon tissue compared to the model group. Our study addressed that HGT can alleviate DSS-induced colitis of mice through inhibiting colonic necroptosis by upregulating the level of VDR.
Keywords	Oriental traditional medicine ; Western blotting ; body weight changes ; cadherins ; caspase-8 ; colon ; dextran sulfate ; diarrhea ; fluorescent antibody technique ; histology ; interleukin-6 ; mice ; models ; necroptosis ; occludins ; pain ; protective effect ; protein content ; therapeutics ; ulcerative colitis
Language	English
Dates of publication	2022-0816
Publishing place	Elsevier B.V.
Document type	Article
Note	Pre-press version
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2022.115655
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Article ; Online: Berberine in the treatment of ulcerative colitis: A possible pathway through Tuft cells.

Xiong, Xinyu / Cheng, Zhe / Wu, Fan / Hu, Meilin / Liu, Zhimin / Dong, Ruolan / Chen, Guang

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

2020 Volume 134, Page(s) 111129

Abstract: Ulcerative colitis (UC) is an inflammatory bowel disease with complex pathogenesis, which is affected by genetic factors, intestinal immune status and intestinal microbial homeostasis. Intestinal epithelial barrier defect is crucial to the development of ...

Abstract	Ulcerative colitis (UC) is an inflammatory bowel disease with complex pathogenesis, which is affected by genetic factors, intestinal immune status and intestinal microbial homeostasis. Intestinal epithelial barrier defect is crucial to the development of UC. Berberine, extracted from Chinese medicine, can identify bitter taste receptor on intestinal Tuft cells and activate IL-25-ILC2-IL-13 immune pathway to impair damaged intestinal tract by promoting differentiation of intestinal stem cells, which might be a potential approach for the treatment of UC.
MeSH term(s)	Animals ; Anti-Inflammatory Agents/therapeutic use ; Berberine/therapeutic use ; Colitis, Ulcerative/drug therapy ; Colitis, Ulcerative/immunology ; Colitis, Ulcerative/metabolism ; Colitis, Ulcerative/pathology ; Colon/drug effects ; Colon/immunology ; Colon/metabolism ; Colon/pathology ; Cytokines/metabolism ; Humans ; Inflammation Mediators/metabolism ; Intestinal Mucosa/drug effects ; Intestinal Mucosa/metabolism ; Intestinal Mucosa/pathology ; Receptors, G-Protein-Coupled/agonists ; Receptors, G-Protein-Coupled/metabolism ; Signal Transduction ; Stem Cells/drug effects ; Stem Cells/immunology ; Stem Cells/metabolism ; Stem Cells/pathology
Chemical Substances	Anti-Inflammatory Agents ; Cytokines ; Inflammation Mediators ; Receptors, G-Protein-Coupled ; taste receptors, type 1 ; taste receptors, type 2 ; Berberine (0I8Y3P32UF)
Language	English
Publishing date	2020-12-24
Publishing country	France
Document type	Journal Article ; Review
ZDB-ID	392415-4
ISSN	1950-6007 ; 0753-3322 ; 0300-0893
ISSN (online)	1950-6007
ISSN	0753-3322 ; 0300-0893
DOI	10.1016/j.biopha.2020.111129
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Article ; Online: Traditional herbal pair Portulacae Herba and Granati Pericarpium alleviates DSS-induced colitis in mice through IL-6/STAT3/SOCS3 pathway.

Cheng, Zhe / Zhou, Yi / Xiong, Xinyu / Li, Lingli / Chen, Zekai / Wu, Fan / Dong, Ruolan / Liu, Qiong / Zhao, Yan / Jiang, Shujun / Yu, Qin / Chen, Guang

Phytomedicine : international journal of phytotherapy and phytopharmacology

2023 Volume 126, Page(s) 155283

Abstract: Background: Portulacae Herba and Granati Pericarpium pair (PGP) is a traditional Chinese herbal medicine treatment for colitis, clinically demonstrating a relatively favorable effect on relieving diarrhea and abnormal stools. However, the underlying ... ...

Abstract	Background: Portulacae Herba and Granati Pericarpium pair (PGP) is a traditional Chinese herbal medicine treatment for colitis, clinically demonstrating a relatively favorable effect on relieving diarrhea and abnormal stools. However, the underlying mechanism remain uncertain. Purpose: The present study intends to evaluate the efficacy of PGP in treating colitis in mice and investigate its underlying mechanism. Methods: The protective effect of PGP against colitis was determined by monitoring body weight, colon length, colon weight, and survival rate in mice. Colonic inflammation was assessed by serum cytokine levels, colonic H&E staining, and local neutrophil infiltration. The reversal of intestinal epithelial barrier damage by PGP was subsequently analyzed with Western blot and histological staining. Furthermore, RNA-seq analysis and molecular docking were performed to identify potential pathways recruited by PGP. Following the hints of the transcriptomic results, the role of PGP through the IL-6/STAT3/SOCS3 pathway in DSS-induced colitis mice was verified by Western blot. Results: DSS-induced colitis in mice was significantly curbed by PGP treatment. PGP treatment significantly mitigated DSS-induced colitis in mice, as evidenced by improvements in body weight, DAI severity, survival rate, and inflammatory cytokines levels in serum and colon. Moreover, PGP treatment up-regulated the level of Slc26a3, thereby increasing the expressions of the tight junction/adherens junction proteins ZO-1, occludin and E-cadherin in the colon. RNA-seq analysis revealed that PGP inhibits the IL-6/STAT3/SOCS3 pathway at the transcriptional level. Molecular docking indicated that the major components of PGP could bind tightly to the proteins of IL-6 and SOCS3. Meanwhile, the result of Western blot revealed that the IL-6/STAT3/SOCS3 pathway was inhibited at the protein level after PGP administration. Conclusion: PGP could alleviate colonic inflammation and reverse damage to the intestinal epithelial barrier in DSS-induced colitis mice. The underlying mechanism involves the inhibition of the IL-6/STAT3/SOCS3 pathway.
MeSH term(s)	Animals ; Mice ; Interleukin-6/metabolism ; Molecular Docking Simulation ; Colitis/chemically induced ; Colitis/drug therapy ; Colitis/metabolism ; Inflammation/metabolism ; Colon/pathology ; Cytokines/metabolism ; Body Weight ; Dextran Sulfate/adverse effects ; Mice, Inbred C57BL ; Disease Models, Animal ; Colitis, Ulcerative/drug therapy ; Sulfate Transporters/metabolism ; Sulfate Transporters/pharmacology ; Sulfate Transporters/therapeutic use ; Antiporters/adverse effects ; Antiporters/metabolism ; Pomegranate ; Plant Extracts
Chemical Substances	Interleukin-6 ; pomegranate fruit rind (RS999V57DU) ; Cytokines ; Dextran Sulfate (9042-14-2) ; Slc26a3 protein, mouse ; Sulfate Transporters ; Antiporters ; Plant Extracts
Language	English
Publishing date	2023-12-18
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1205240-1
ISSN	1618-095X ; 0944-7113
ISSN (online)	1618-095X
ISSN	0944-7113
DOI	10.1016/j.phymed.2023.155283
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Article: Ameliorative Effects of Osthole on Experimental Renal Fibrosis

Wu, Fan / Zhao, Yan / Shao, Qingqing / Fang, Ke / Dong, Ruolan / Jiang, Shujun / Lu, Fuer / Luo, Jinlong / Chen, Guang

Frontiers in pharmacology

2021 Volume 12, Page(s) 646331

Abstract: Objectives: ...

Abstract	Objectives:
Language	English
Publishing date	2021-05-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2021.646331
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